Modern Pharmaceutical Analytical Techniques (CUTM1583)
Teacher
Dr. BIKASH RANJAN JENA, M.Pharm., Ph.D
Category
Core Courses
Course Attendees
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Course Reviews
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Scope
This subject deals with various advanced analytical instrumental techniques for
identification, characterization and quantification of drugs. Instruments dealt are
NMR, Mass spectrometer, IR, HPLC, GC etc.
Course Objectives
After completion of course student is able to know,
Chemicals and Excipients
The analysis of various drugs in single and combination dosage forms
Theoretical and practical skills of the instruments
Modern Pharmaceutical Analytical Techniques
Course Outcome:
| CO | Statement | Mapping with POs |
| CO-1 | Understand the interaction of matter with electromagnetic radiations and its applications in drug analysis | PO1, PO2, PO8 |
| CO-2 | Understand the chromatographic separation and analysis of drugs. | PO1, PO6, PO9 |
| CO-3 | Perform quantitative & qualitative analysis of drugs using various analytical instruments. | PO1, PO2, PO8 |
| CO-4 | Able to separate and analyses drugs by chromatography Radioimmunoassay and Electrophoresis | PO1, PO3, PO6, PO8 |
| CO-5 | student interpretation skills will be improve by the course content in terms of choice of analytical techniques to perform the estimation of different category drugs. | PO1, PO2, PO6 |
SYLLABUS [THEORY]
1. a. UV-Visible spectroscopy: Introduction, Theory, Laws,
Instrumentation associated with UV-Visible spectroscopy, Choice of solvents and solvent effect and Applications of UV-Visible spectroscopy.
b. IR spectroscopy: Theory, Modes of Molecular vibrations,
Sample handling, Instrumentation of Dispersive and Fourier - Transform IR Spectrometer, Factors affecting vibrational frequencies and Applications of IR spectroscopy
c. Spectroflourimetry: Theory of Fluorescence, Factors affecting fluorescence, Quenchers, Instrumentation and Applications of fluorescence spectrophotometer.
d. Flame emission spectroscopy and Atomic absorption
spectroscopy: Principle, Instrumentation, Interferences and Applications.
2. NMR spectroscopy: Quantum numbers and their role in NMR,
Principle, Instrumentation, Solvent requirement in NMR, Relaxation process, NMR signals in various compounds,
Chemical shift, Factors influencing chemical shift, Spin-Spin coupling, Coupling constant, Nuclear magnetic double resonance, Brief outline of principles of FT-NMR and 13C NMR. Applications of NMR spectroscopy.
3. Mass Spectroscopy: Principle, Theory, Instrumentation of Mass
Spectroscopy, Different types of ionization like electron impact, chemical, field, FAB and MALDI, APCI, ESI, APPI Analyzers of Quadrupole and Time of Flight, Mass fragmentation and its rules, Meta stable ions, Isotopic peaks and Applications of Mass
spectroscopy
4. Chromatography: Principle, apparatus, instrumentation, chromatographic parameters, factors affecting resolution and applications of the following:
a) Paper chromatography b) Thin Layer chromatography
c) Ion exchange chromatography d) Column chromatography
e) Gas chromatography f) High Performance Liquid
chromatography
g) Affinity chromatography
5 a. Electrophoresis: Principle, Instrumentation, Working
conditions, factors affecting separation and applications of the
following:
a) Paper electrophoresis b) Gel electrophoresis c) Capillary
electrophoresis d) Zone electrophoresis e) Moving boundary
electrophoresis f) Iso electric focusing
b. X ray Crystallography: Production of X rays, Different X ray
diffraction methods, Bragg‘s law, Rotating crystal technique, X
ray powder technique, Types of crystals and applications of Xray
diffraction.
6. Immunological assays: RIA (Radio immuno assay), ELISA,
Bioluminescence assays.
REFERENCES:
1. Spectrometric Identification of Organic compounds - Robert M Silverstein,
Sixth edition, John Wiley & Sons, 2004.
2. Principles of Instrumental Analysis - Doglas A Skoog, F. James Holler,
Timothy A. Nieman, 5th edition, Eastern press, Bangalore, 1998.
3. Instrumental methods of analysis – Willards, 7th edition, CBS publishers.
4. Practical Pharmaceutical Chemistry – Beckett and Stenlake, Vol II, 4th
edition, CBS Publishers, New Delhi, 1997.
5. Organic Spectroscopy - William Kemp, 3rd edition, ELBS, 1991.
6. Quantitative Analysis of Drugs in Pharmaceutical formulation - P D Sethi, 3rd
Edition, CBS Publishers, New Delhi, 1997.
7. Pharmaceutical Analysis- Modern methods – Part B - J W Munson, Volume
11, Marcel Dekker Series
Session 1: Concept of UV visible spectroscopy, theory and Laws of UV spectroscopy
Session 2: Instrumentation, effect of Solvents and App;ications of UV
Session 3: Introduction to IR spectroscopy
Infrared spectroscopy _ HiQ (Introduction, theory)
Session 4: Instrumentation of IR spectroscopy
Session 5: Types of vibrations to IR spectroscopy and its Applications
Session 6: Spectroflourimetry: Theory of Fluorescence, Factors
affecting fluorescence, Quenchers, Instrumentation
Session 7: Flame emission spectroscopy and Atomic absorption
spectroscopy: Principle, Instrumentation and Applications
flame phtometry (Principle, theory)
Session 8: Flame Photometry Instrumentation and Applications
Session 9: NMR spectroscopy: Quantum numbers and their role in NMR,
Principle, Instrumentation, Solvent requirement in NMR,
Relaxation process, Applications
Session 10: Nuclear magnetic double resonance,
Brief outline of principles of FT-NMR and 13C NMR. Applications
of NMR spectroscopy.
Session 11:
Chemical shift, Factors influencing chemical shift, Spin-Spin coupling, Coupling constant in NMR spectroscopy
Session 14
Instrumentation and Application's of C13 NMR
Session 15
Mass Spectroscopy: Principle, Theory of Mass spectroscopy.
https://yunus.hacettepe.edu.tr/~selis/teaching/WEBkmu396/ppt/KMU396Presentations2011/MS.pdf
Session 16
Instrumentation of Mass Spectroscopy
Session 17
Different types of ionization like electron impact, chemical ionization.
Session 18
FAB and APCI, ESI, APPI Analyzers of Quadrupole and Time of Flight, Mass fragmentation and its rules, Meta stable ions, Isotopic peaks and Applications of Mass spectroscopy.
Electrospray_Ionisation_Mass_Spectrometry_Principl
Session 21
Principle and instrumentation of Ion exchange chromatography .
Session 22
Principle and instrumentation of High Performance Liquid chromatography. (HPLC).
Session 27
Application's of X ray crystallography and Bragg's law of X ray diffraction
Session 28
Principle and instrumentation and Application's of X ray crystallography
Session 30
Application's of X ray crystallography and Bragg's law of X ray diffraction
Our Main Teachers
About Me My self Dr.Bikash Ranjan Jena currently working in Pharmaceutical Analysis & Research Division, School of Pharmacy and Life Sciences (SoPLS), Centurion University, Bhubaneswar since Jan 2021. I have successfully awarded my Ph.D degree at KLEF Deemed to be University, Vaddeswaram, Guntur, A.P on 22nd June 2023. Expertised in Analytical Quality by Design (AQbD), […]