SUBJECT: MODERN PHARMACEUTICAL ANALYTICAL TECHNIQUES
Course Code: CUTM2524
Course Type: Core Course
Modern Pharmaceutical Analytical Techniques (CUTM2424)
Teacher
Dr. BIKASH RANJAN JENA, M.Pharm., Ph.D
Category
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Course Reviews
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Course Objectives:
After completion of course student is able to know
- The analysis of various drugs in single and combination dosage forms
- Theoretical and practical skills of the instruments
Course Outcome:
| CO | Statement | Mapping with POs |
| CO-1 | Understand the interaction of matter with electromagnetic radiations and its applications in drug analysis | PO1, PO2, PO8 |
| CO-2 | Understand the chromatographic separation and analysis of drugs. | PO1, PO6, PO9 |
| CO-3 | Perform quantitative & qualitative analysis of drugs using various analytical instruments. | PO1, PO2, PO8 |
| CO-4 | Able to separate and analyses drugs by chromatography Radioimmunoassay and Electrophoresis | PO1, PO3, PO6, PO8 |
| CO-5 | student interpretation skills will be improve by the course content in terms of choice of analytical techniques to perform the estimation of different category drugs. | PO1, PO2, PO6 |
Scope
This subject deals with various advanced analytical instrumental techniques for identification, characterization and quantification of drugs. Instruments dealt are NMR, Mass spectrometer, IR, HPLC, GC etc.
THEORY
1. UV-Visible spectroscopy: Introduction, Theory, Laws, Instrumentation associated with UV-Visible spectroscopy, Choice of solvents and solvent effect and Applications of UV-Visible spectroscopy.
IR spectroscopy: Theory, Modes of Molecular vibrations, Sample handling, Instrumentation of Dispersive and Fourier - Transform IR Spectrometer, Factors affecting vibrational frequencies and Applications of IR spectroscopy
Spectroflourimetry: Theory of Fluorescence, Factors affecting fluorescence, Quenchers, Instrumentation and Applications of fluorescence spectrophotometer.
Flame emission spectroscopy and Atomic absorption spectroscopy: Principle, Instrumentation, Interferences and Applications.
- NMR spectroscopy: Quantum numbers and their role in NMR, Principle, Instrumentation, Solvent requirement in NMR, Relaxation process, NMR signals in various compounds, Chemical shift, Factors influencing chemical shift, Spin-Spin coupling, Coupling constant, Nuclear magnetic double resonance, Brief outline of principles of FT-NMR and 13C NMR. Applications of NMR spectroscopy.
- Mass Spectroscopy: Principle, Theory, Instrumentation of Mass Spectroscopy, Different types of ionization like electron impact, chemical, field, FAB and MALDI, APCI, ESI, APPI Analyzers of Quadrupole and Time of Flight, Mass fragmentation and its rules, Meta stable ions, Isotopic peaks and Applications of Mass spectroscopy
- Chromatography: Principle, apparatus, instrumentation, chromatographic parameters, factors affecting resolution and applications of the following:
- a) Paper chromatography b) Thin Layer chromatography
- c) Ion exchange chromatography d) Column chromatography
- e) Gas chromatography f) High Performance Liquid chromatography
- g) Affinity chromatography
5.Electrophoresis: Principle, Instrumentation, Working conditions, factors affecting separation and applications of the following:
a) Paper electrophoresis b) Gel electrophoresis c) Capillary electrophoresis d) Zone electrophoresis e) Moving boundary electrophoresis f) Iso electric focusing .
b) X ray Crystallography: Production of X rays, Different X ray methods, Bragg‘ s law, Rotating crystal technique, X ray powder technique, Types of crystals and applications of X-ray diffraction.
6.Potentiometry: Principle, working, Ion selective Electrodes and Application of potentiometry. Thermal Techniques: Principle, thermal transitions and Instrumentation (Heat flux and power-compensation and designs), Modulated DSC, Hyper DSC, experimental parameters (sample preparation, experimental conditions, calibration, heating and cooling rates, resolution, source of errors) and their influence, advantage and disadvantages, pharmaceutical applications. Differential Thermal Analysis (DTA): Principle, instrumentation 99 10 Hrs and advantage and disadvantages, pharmaceutical applications, derivative differential thermal analysis (DDTA). TGA: Principle, instrumentation, factors affecting results, advantage and disadvantages, pharmaceutical applications.
REFERENCES
- Spectrometric Identification of Organic compounds - Robert M Silverstein, 6th edition, John Wiley & Sons, 2004.
- Principles of Instrumental Analysis Doglas A Skoog, F. James Holler, Timothy A. Nieman, 5th edition, Eastern press, Bangalore, 1998.
- Instrumental methods of analysis – Willards, 7th edition, CBS publishers.
- Practical Pharmaceutical Chemistry – Beckett and Stenlake, Vol II, 4th edition, CBS Publishers, New Delhi, 1997.
- Organic Spectroscopy - William Kemp, 3rd edition, ELBS, 1991.
- Quantitative Analysis of Drugs in Pharmaceutical formulation - P D Sethi, 3rd Edition, CBS Publishers, New Delhi, 1997.
- Pharmaceutical Analysis- Modern methods – Part B - J W Munson, Volume 11, Marcel Dekker Series
- Spectroscopy of Organic Compounds, 2nd edn., P.S/Kalsi, Wiley estern Ltd., Delhi. 9. Textbook of Pharmaceutical Analysis, KA.Connors, 3rd Edition, John Wiley & Sons, 1982.
Session 1: Introduction to UV visibile spectroscoopy
Session 2: UV visibile spectroscoopy, instrumentation and Applications
Session 3: Theory, Modes of Molecular vibrations,
Sample handling,
Session 4: Instrumentation and Applications of IR spectroscopy
Infrared spectroscopy _ (Instrumentation, Different types of Sample handlings)
Session 5: Molecular vibrations and Applications of IR spectroscopy
IR spectroscopy (Multivariate Applications)
Session 6: Flame emission spectroscopy: Principle, Instrumentation, Interferences
Session 7: Spectroflourimetry: Theory of Fluorescence, Factors
affecting fluorescence, Quenchers,
Spectro-flourimetry (Principle, Theory, Instrumentations, Applications)
Session 8: Instrumentation, Interferences and
Applications.
Session 9:
NMR spectroscopy: Quantum numbers and their role in NMR,
Principle, Instrumentation, Solvent requirement in NMR,
Relaxation process.
Session 10:
NMR spectroscopy: Nuclear magnetic double resonance,
Brief outline of principles of FT-NMR and 13C NMR. Applications
of NMR spectroscopy.
Session 11:
Chemical shift, Factors influencing chemical shift, Spin-Spin coupling, Coupling constant in NMR spectroscopy
Session 14
Instrumentation and Application's of C13 NMR
Session 15
Mass Spectroscopy: Principle, Theory of Mass spectroscopy.
https://yunus.hacettepe.edu.tr/~selis/teaching/WEBkmu396/ppt/KMU396Presentations2011/MS.pdf
Session 16
Instrumentation of Mass Spectroscopy
Session 17
Different types of ionization like electron impact, chemical ionization.
Session 18
FAB and APCI, ESI, APPI Analyzers of Quadrupole and Time of Flight, Mass fragmentation and its rules, Meta stable ions, Isotopic peaks and Applications of Mass spectroscopy.
Electrospray_Ionisation_Mass_Spectrometry_Principl
Session 21
Principle and instrumentation of Ion exchange chromatography .
Session 22
Principle and instrumentation of High Performance Liquid chromatography. (HPLC).
Session 27
Principle and instrumentation and Application's of Moving boundary electrophoresis and Iso electric focusing.
Session 28
Principle and instrumentation and Application's of X ray crystallography
Session 29
Application's of X ray crystallography and Bragg's law of X ray diffraction
Our Main Teachers
About Me My self Dr.Bikash Ranjan Jena currently working in Pharmaceutical Analysis & Research Division, School of Pharmacy and Life Sciences (SoPLS), Centurion University, Bhubaneswar since Jan 2021. I have successfully awarded my Ph.D degree at KLEF Deemed to be University, Vaddeswaram, Guntur, A.P on 22nd June 2023. Expertised in Analytical Quality by Design (AQbD), […]