Molecular Biology

Teacher

Miss Sonali Dash

Category

Core Courses

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Course Name : Molecular Biology

Code(Credit) :  CUTM1737(3-0-1)

Course Objectives

  • Understanding of chemical and  molecular processes that occur in and between cells.   Understanding will become such that , can be able to describe and explain processes and their meaning for the characteristics of living organisms.
  • To provide depth knowledge of biological and medicinal processes through the investigation of the underlying molecular mechanisms.

Learning Outcomes

  • Conduct independent work in a laboratory.
  • Read scientific articles and gain a critical understanding of their contents.
  • Give a spoken and written presentation of scientific topics and research results.
  • Present hypotheses and select, adapt and conduct molecular and cell-based experiments to either confirm or reject the hypotheses.

Course Syllabus

Module I

Introduction: a. Introduction to molecular biology, b. Molecular biology of cell.Evolution and Molecular structure of cell and its organelles. Types of cells. Including different kinds of Prokaryotic and eukaryotic cells, Cell growth, Cell adhesion, cell junctions and extra cellular matrix organelles, Cell cycle, Cell membrane and its structure (fluid-mosaic model). Factors influencing on membrane fluidity, asymmetry of membrane and membrane transport (active and passive)

Project Topic

Causes, types, and molecular mechanism of human cancer.

Module II

Molecular Nature of the Genetic Material in Prokaryotic and Eukaryotic Cells: Molecular biology of Genes, DNA: Molecular structure, types: Primary, secondary and tertiary, Double helix, types,  Transferring information from DNA to RNA, Synthesis of RNA, Translation RNA: Molecular structure, types. Evolution of DNA and RNA, Gene and genetic codes.

Project Topic

Tumor suppressor gene and oncogene.

Module III

General Concept on: a. Regulation of the Gene Expression b. Regulating the Metabolism: The Lac- Operon system, Catabolic repression, Trp Operon system: regulating the biosynthesis of the tryptophan, Gene expression in Eukaryotic cells, Plasmids: types, maintenance and functions.

Project Topic

Human Genome project.

Module IV

DNA Replication and Gene Expression: DNA Replication: Semi conservative Nature of DNA Replication,DNA Replication in prokaryotic Cells,DNA Replication in Eukaryotic cell, Enzymes involved in DNA Replication: DNA polymerases, Proofreading, post-replication Modification of DNA. Transferring information from DNA to RNA, Synthesis of RNA (Transcription), RNA polymerase, Initiation and Termination of Transcription, Post and co- transcription modification of the RNA. Protein Biosynthesis: Translation of the genetic code, Translation of m RNA, Role of r-RNA in protein synthesis, Forming the polypeptides- elongation,Termination of the protein biosynthesis.

Project Topic

Molecular basis, types, causes and a case study of the effects of DNA mutation.

Suggested Readings

1.Watson JD, Baker TA, Bell SP, Gann A, Levine M and Losick R (2008) Molecular Biology of the Gene, 7th edition, Cold Spring Harbour Lab. Press, Pearson Publication.

Ebook Link- https://www.pdfdrive.com/molecular-biology-of-the-gene-e158278674.html

2.Molecular cell biology (5th Ed) by Lodish H.

Ebook Link- https://www.pdfdrive.com/molecular-cell-biology-lodish-5th-ed-e15674865.html

3. Genes XII by Lewin's.

Ebook Link- https://www.pdfdrive.com/lewins-genes-xii-e168024578.html

Online Tutorial Links

DNA Replication.

Elink- https://app.lecturio.com/#/course/s/8020

Transcription Process in eukaryotes and prokaryotes.

Elink-https://app.lecturio.com/#/lecture/s/5990/35832

Session Plan

Session 1

Lecture Topic- Introduction to molecular biology,

Lecture Video- https://www.youtube.com/watch?v=sZnK6rz4Iks

Lecture Notes- https://www.slideshare.net/abhijedi123/molecular-biology-introduction

Lecture Readings-

Watson pg.21- Chapter 2

Session 2

Lecture Topic- Molecular biology of cell. Evolution and Molecular structure of cell and its organelles.

Lecture Video- https://www.youtube.com/watch?v=URUJD5NEXC8

Lecture Notes- http://www.uou.ac.in/sites/default/files/slm/BSCZO-102.pdf

Session 3

Lecture Topic- Types of cells. Including different kinds of Prokaryotic and eukaryotic cell.

Lecture Video- https://www.youtube.com/watch?v=QSfEA_Wxl7g.

https://www.youtube.com/watch?v=zZtcMBTQaS4

Lecture Presentation- https://www.slideshare.net/EasyBiologyClassEBC/difference-between-prokaryotes-and-eukaryotes-ppt-by-easybiologyclass

Session 5

Lecture Topic- Cell junctions.

Lecture Video- https://www.youtube.com/watch?v=Sr_Ok-DaWXg

Lecture Presentation- https://www.slideshare.net/elsavonlicy/cell-junctions-2013

Session 8

Lecture Topic- Cell membrane and its structure (fluid-mosaic model) and Factors influencing on membrane fluidity.

Lecture Video- https://www.youtube.com/watch?v=Qqsf_UJcfBc

https://www.youtube.com/watch?v=CNbZDcibegY

Lecture Presentation- https://www.slideshare.net/jayak1/the-fluid-mosaic-model-of-membrane-structure

Lecture Readings- Lodish pg .148- chapter 5.

Session 9

Lecture Topic-Asymmetry of membrane and membrane transport (active and passive transport).

Lecture Video- https://www.youtube.com/watch?v=ufCiGz75DAk

Lecture Presentation- https://www.slideshare.net/bethgombert/passive-and-active-transport

Lecture Readings-

Lodish pg .245- chapter 7.

Session 10

Project Topic- Causes, types, and molecular mechanism of human cancer.

Session 11

Lecture Topic- Molecular Nature of the Genetic Material in Prokaryotic and Eukaryotic Cells.

Lecture Video- https://www.youtube.com/watch?v=X6KXBZ9je2o

Lecture Notes- https://www.slideshare.net/biruthapa/dna-organization-or-genetic-makeup-in-prokaryotic-and-eukaryotic-systems

Session 12

Lecture Topic- Molecular biology of Genes, DNA and chromosome.

Lecture Video- https://www.youtube.com/watch?v=mcEV3m9SG9M

https://www.youtube.com/watch?v=exDNUzKf9tU

Lecture Presentation- https://www.slideshare.net/aqsa123456/dna-chromosomes-genes-23918366

Lecture Readings- Watson pg .77- chapter 4.

Session 13

Lecture Topic- DNA: Molecular structure, types: Primary, secondary and tertiary, Double helix.

Lecture Video- https://www.youtube.com/watch?v=LCMc8_xSlkQ

https://www.youtube.com/watch?v=MCKI0PJJ4uA

LecturePresentation- https://www.slideshare.net/ganeshgaonkar3/watson-and-crick-model-of-dna

Lecture Readings-Watson pg .78- chapter 4.

Session 14

Lecture Topic- RNA: Molecular structure, Types of RNA and Evolution of DNA and RNA.

Lecture Video-https://youtu.be/D3ta4dObwg8

https://www.youtube.com/watch?v=K1xnYFCZ9Yg

Lecture Presentation-. https://youtu.be/D3ta4dObwg8 https://www.slideshare.net/namarta28/rna

Lecture Readings- Watson pg .107-chapter 5.

Session 15

Lecture Topic- Gene and genetic codes.

Lecture Video- https://www.youtube.com/watch?v=a48GfC0ygpg

Lecture Presentation- https://www.genome.gov/genetics-glossary/Genetic-Code

Lecture Readings-Watson pg .573- chapter 16.

Session 16

Project Topic- Tumor suppressor gene and oncogene.

Session 17

Lecture Topic- General Concept on: a. Regulation of the Gene Expression.

Lecture Video- https://www.coursera.org/lecture/papers-molecular-genetics/part-7-8Pv2U

Lecture Presentation- https://www.slideshare.net/gohilsanjay3/regulation-of-gene-expression-in-prokaryotes-122874320

Lecture Readings- Watson pg.418-Chapter 18.

Session 18

Lecture Topic- Regulating the Metabolism:The Lac- Operon system.

Lecture Video- https://www.coursera.org/lecture/papers-molecular-genetics/part-6-DtVas

Lecture Presentation- https://www.slideshare.net/TapeshwarYadav1/lac-operon-56015047

Lecture Readings- Watson Pg.421-Chapter 18.

Session 19

Lecture Topic- Catabolic repression, Trp Operon system.

Lecture Video-https://www.coursera.org/lecture/papers-molecular-genetics/part-1-E13k9

Lecture Notes- https://www.slideshare.net/georgeoajr/trp-operon-107712378

Session 20

Lecture Topic- Gene expression in Eukaryotic cells.

Lecture Video- https://www.coursera.org/lecture/genes/gene-expression-Ob3FQ

Lecture Presentations- https://www.slideshare.net/gangadharchatterjee/regulation-of-gene-expression-in-eukaryotes-66391915

Lecture Readings- Watson pg. 657- Chapter 19.

Session 21

Session 22

Project Topic-Human Genome project.

Session 23

Lecture Topic- DNA Replication: Semi conservative Nature of DNA Replication,DNA Replication in prokaryotic Cells.

Lecture Video-https://www.coursera.org/lecture/dna-decoded/dna-replication-f2DcN

https://pt.coursera.org/lecture/dna-decoded/stop-copying-me-dna-replication-Z9jkE

Lecture Presentations- https://www.slideshare.net/RIZWANABBAS3/replication-in-prokaryotes

Lecture Readings-Watson pg .257- chapter 9.

Session 24

Lecture Topic- DNA Replication in Eukaryotic cell and Enzymes involved in DNA Replication.

Lecture Video- https://www.coursera.org/lecture/bioinformatics/optional-where-in-the-genome-does-dna-replication-begin-part-3-mA25X

https://www.udemy.com/course/dna-molecular-structure-replication

Lecture Presentation- https://www.slideshare.net/MVignesh2/dna-replication-in-eukaryotes

Lecture Readings- Watson pg .257- chapter 9.

Session 25

Lecture Topic- DNA polymerases and proof reading.

Lecture Video-https://www.youtube.com/watch?v=1ZuomrTCOOo

Lecture Presentation- https://courses.lumenlearning.com/wm-biology1/chapter/reading-proofreading-dna/

Session 26

Lecture Topic- Post-replication Modification of DNA.

Lecture Video- https://www.coursera.org/lecture/toxicology-21/microrna-and-epigenetics-N2Ng6

Lecture Readings-Watson pg .467- chapter 14.

Session 27

Lecture Topic- Transferring information from DNA to RNA( transcription) in prokaryotes.

Lecture Video- https://www.coursera.org/lecture/data-genes-medicine/transcription-process-KoaKd

Lecture Notes- https://www.slideshare.net/gohilsanjay3/rna-polymerase-transcription-in-prokaryotes

Lecture Readings-Watson pg .265- chapter 9.

Session 28

Lecture Topic- RNA polymerase, Initiation and Termination of Transcription.

Lecture Video- https://www.youtube.com/watch?v=nJK-l7ByQAs

Lecture Presentation-https://www.slideshare.net/Hemantkrdu/transcription-in-eukaryotes-60971263

Lecture Readings-Watson pg .302- chapter 9.

Session 29

Lecture Topic- Post and co- transcription modification of the RNA.

Lecture Video- https://www.coursera.org/lecture/introduction-genomics/why-genomics-wsP6i

Lecture Notes- https://www.slideshare.net/narasimhareddy3954546/post-transcriptional-modifications

Lecture Readings-Watson pg .467- chapter 14.

Session 31

Lecture Topic- Forming the polypeptides- Initiation, elongation,Termination.

Lecture Video-https://www.udemy.com/course/rna-structure-and-protein-synthesis-mcq-bio-chemistry/

Lecture Presentation- https://teachmephysiology.com/basics/protein-synthesis/dna-translation/

Lecture Readings- Watson pg .531- chapter 15.

Session 32

Project Topic-Molecular basis, types, causes and a case study of the effects of DNA mutation.

Session 33

Revision and Discussion Class

Case Studies

Case Studies

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