MYCOLOGY AND PHYTOPATHOLOGY

Teacher

Dr. Pratibha Rani Deep

Category

Core Courses

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Course Name : Mycology and Phytopathology

Code(Credit) : CUTM1457 (3-2-1)

Course Objectives

Understanding the principles of plant pathology and the application of principles in the control of plant disease.

 

To understand of how human as well plant life is highly dependent upon the microbes present in environment.

 

Gain knowledge on Fungi, their structure and function, their effect on living world, economic importance etc.

Learning Outcomes

Will have thorough knowledge on the science of plant pathology such as causal agents of plant diseases, symptoms and diagnoses, modes of infection and spread, effects of the environment on disease development, and methods of disease control.

 

Knowledge of the strategies used for conventional and novel control of plant diseases management with the help of molecular tools to understand these mechanisms and phenomena.

 

Know about plant’s defense mechanisms against this diseases.

Course Syllabus

Module-I

Introduction to true fungi: General characteristics; Affinities with plants and animals; Thallus organization; Cell wall composition; Nutrition; Classification.

Chytridiomycota and Zygomycota: Characteristic features; Ecology and significance; Thallus organisation; Reproduction; Life cycle with reference to Synchytrium, Rhizopus.

Practice 1: Introduction to the world of fungi (Unicellular, coenocytic / septate mycelium, ascocarps & basidiocarps).

Practice 2:Rhizopus: study of asexual stage from temporary mounts and sexual structures through permanent slides.

 

Module-II

Ascomycota: General characteristics (asexual and sexual fruiting bodies); Ecology; Life cycle, Heterokaryosis and parasexuality; Life cycle and classification with reference to Saccharomyces, Aspergillus, Penicillium, Alternaria, Neurospora and Peziza.

Basidiomycota : General characteristics; Ecology; Life cycle and Classification with reference to black stem rust on wheat Puccinia (Physiological Specialization), loose and covered smut (symptoms only), Agaricus; Bioluminescence, Fairy Rings and Mushroom Cultivation.

Practice 3: Aspergillus and Penicillium: study of asexual stage from temporary mounts. Study of Sexual stage from permanent slides/photographs.

Practice 4: Alternaria: Specimens/photographs and temporary mounts

Practice 5:Peziza: sectioning through ascocarp.

Practice 6Puccinia: Herbarium specimens of Black Stem Rust of Wheat and infected Barberry leaves; sections/mounts of spores on wheat and permanent slides of both the hosts.

Practice 7: Agaricus: Specimens of button stage and full grown mushroom; sectioning of gills of Agaricus, fairy ringsand bioluminescent mushrooms to be shown.

 

Module-III

Allied Fungi: General characteristics; Status of Slime molds, Classification; Occurrence; Types of plasmodia; Types of fruiting bodies.

Oomycota: General characteristics; Ecology; Life cycle and classification with reference to

Phytophthora, Albugo.

Practice 8: Study of phaneroplasmodium from actual specimens and/or photograph. Study of Stemonitis sporangia.

Practice 9: Albugo: Study of symptoms of plants infected with Albugo; asexual phase study through section/ temporary mounts and sexual structures through permanent slides

 

Module-IV

Symbiotic associations: Lichen–Occurrence; General characteristics; Growth forms and range of thallus organization; Nature of associations of algal and fungal partners; Reproduction; Mycorrhiza- Ectomycorrhiza, Endomycorrhiza and their significance.

Practice 10:Lichens: Study of growth forms of lichens (crustose, foliose and fruticose) on different substrates. Study of thallus and reproductive structures (soredia and apothecium) through permanent slides. Mycorrhizae: ectomycorrhiza and endomycorrhiza (Photographs)

Practice 11: Study of Mycorrhizae: ectomycorrhiza and endomycorrhiza (Photographs)

 

Module-V

Applied Mycology: Role of fungi in biotechnology; Application of fungi in food industry (Flavour & texture, Fermentation, Baking, Organic acids, Enzymes, Mycoproteins); Secondary metabolites(Pharmaceutical preparations); Agriculture (Bio-fertilizers); Mycotoxins; Biological control (Mycofungicides, Mycoherbicides, Mycoinsecticides, Myconematicides).

 

Module-VI

Phytopathology: Terms and concepts; General symptoms of diseases; Etiology; Host-Pathogen relationships; Disease cycle and environmental relation; prevention and control of plant diseases, and role of quarantine.

 

Module-VII

Bacterial diseases: Citrus canker and angular leaf spot of cotton. Viral diseases – Tobacco, Mosaic viruses. Fungal diseases – Early blight of potato, Black stem rust of wheat, White rust of crucifers.

Practice 12:Phytopathology: Herbarium specimens of bacterial diseases; Citrus Canker; Angular leaf spot of cotton, Viral diseases: TMV, Vein clearing, Fungal diseases: Early blight of potato,    Black stem rust of wheat and White rust of crucifers.

 

Text Book: 

Agrios, G.N. (1997) Plant Pathology, 4th edition, Academic Press, U.K.

Alexopoulos, C.J., Mims, C.W., Blackwell, M. (1996). Introductory Mycology, John Wiley & Sons (Asia) Singapore. 4th edition.

Reference Book:

Webster, J. and Weber, R. (2007). Introduction to Fungi, Cambridge University Press, Cambridge. 3rd edition.

Sethi, I.K. and Walia, S.K. (2011). Text book of Fungi and Their Allies, Macmillan Publishers India Ltd.

Sharma, P.D. (2011). Plant Pathology, Rastogi Publication, Meerut, India.

Session 2 (Practice 1)

Introduction to the world of fungi (Unicellular, coenocytic / septate mycelium, ascocarps & basidiocarps).

https://www.youtube.com/watch?v=FcW5tHrbAos

https://www.youtube.com/watch?v=FINafQGTHC0

Session 3 (Assessment 1)

Affinities with plants and animals;

https://www.stuartsumida.com/NATSCI360/360Lect6.PDF

Session 7 (Practice 2)

Rhizopus: study of asexual stage from temporary mounts and sexual structures through permanent slides.

http://cyberspaceandtime.com/OZuFzbNIVUQ.video +related

https://www.youtube.com/watch?v=zRMD8rmd3l8

Session 12 (Practice 3)

Aspergillus and Penicillium: study of asexual stage from temporary mounts. Study of Sexual stage from permanent slides/photographs.

http://cyberspaceandtime.com/OZuFzbNIVUQ.video+related

https://www.youtube.com/watch?v=ffP5guiELY0

Session 14 (Practice 4)

Alternaria: Specimens/photographs and temporary mounts

https://www.youtube.com/watch?v=nrMRPptvFz0

https://www.youtube.com/watch?v=1jmNMCTdODM

Session 16 (Practice 5)

Peziza: sectioning through ascocarp.

https://www.youtube.com/watch?v=jFPJvDzGe8A

Session 18 (Practice 6)

Puccinia: Herbarium specimens of Black Stem Rust of Wheat and infected Barberry leaves; sections/mounts of spores on wheat and permanent slides of both the hosts.

https://www.youtube.com/watch?v=FcW5tHrbAos

Session 23 (Practice 7)

Agaricus: Specimens of button stage and full grown mushroom; sectioning of gills of Agaricus, fairy ringsand bioluminescent mushrooms to be shown.

https://www.youtube.com/watch?v=f__ZbEGkdKI

https://www.youtube.com/watch?v=iQsJGKpYoWM

Session 27 (Practice 8)

Study of phaneroplasmodium from actual specimens and /or photograph. Study of  Stemonitis sporangia.

https://www.youtube.com/watch?v=B8dl_CuwQhk&t=43s

Session 30 (Practice 9)

Albugo: Study of symptoms of plants infected with Albugo; asexual phase study through section/ temporary mounts and sexual structures through permanent slides.

https://www.youtube.com/watch?v=n9MgZ14G6kY

Session 33 (Practice 10)

Lichens: Study of growth forms of lichens (crustose, foliose and fruticose) on different substrates. Study of thallus and reproductive structures (soredia and apothecium) through permanent slides.

https://www.youtube.com/watch?v=koWdimb1Dck

Session 36 (Assignment 6)

Session 49 (Practice 11)

Mycorrhizae: ectomycorrhiza and endomycorrhiza (Photographs)

https://www.youtube.com/watch?v=y_8vHJ2YTzA

Session 52 (Practice 12)

Herbarium specimens of bacterial diseases; Citrus Canker; Angular leaf spot of cotton.

https://www.youtube.com/watch?v=AEoblNLr9fU

https://www.youtube.com/watch?v=FQVglUr1tic

Herbarium specimens of Viral diseases: TMV, Vein clearing, Early blight of potato

https://www.youtube.com/watch?v=vBg3jEbKeNQ

https://www.youtube.com/watch?v=pfBIZAs3STo

https://www.youtube.com/watch?v=pa2FvsH7my8

Fungal diseases: Black stem rust of wheat; White rust of crucifers.

https://www.youtube.com/watch?v=pkL8H2tQtBs

https://www.youtube.com/watch?v=ZMpvmmz37CM&t=35s

Our Main Teachers

Dr. Pratibha Rani Deep

Assisstant Professor in Botany, School of Applied Science, ( Bolangir Campus)
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Dr. Pratibha R. Deep works as an Assistant Professor, Department of Botany, at the Centurion University of Technology and Management, Bolangir Campus, Odisha India. She has received her M.Sc. (Botany) from Gangadhar Meher College (presently University), Sambalpur, India. She qualified GATE twice 2007 and 2012. She did her MTech in Applied Botany from IIT Kharagpur, […]