Course Objectives

• To perform basic laboratory techniques in mycology, to isolate fungus from clinical samples for disease diagnosis.
• To describe the characteristics and diseases caused by pathogenic virus and fungi.
• Understanding different methods of virus cultivation.
• Understanding collection, transportation and preservation method of clinical specimen.

Learning Outcomes

• Students will be able to understand the structure, characteristics and classification of virus and fungus.
• Plan, write and implement research projects in virology and mycology analyze their results and publish these in peer-reviewed journals.
• Coordinate with concerned agencies regarding viral and fungal diseases and their outbreaks.
• Plan and execute epidemiological studies and provide advice in relation to viral diseases.

Course Syllabus

Module I

Introduction to medical mycology,  Basic concepts about superficial and deep Mycoses. Taxonomy and classification and general characteristics of various medically important fungi. Normal fungal flora. Morphological, cultural characteristics of common fungal laboratory contaminants, Culture media used in mycology and staining process.

Practice

To prepare culture media used routinely in mycology. Isolation of fungus from different clinical specimen( microscopical and macroscopical characteristics). To perform all the staining techniques for identification of fungi as mentioned in theory syllabus.

Module II

Direct microscopy in Medical mycology laboratory. Processing of clinical samples for diagnosis of fungal infections i.e. Skin, nail, hair, pus, sputum, CSF and other body fluids. Techniques used for isolation and identification of medically important fungi. Methods for identification of yeast and mould.

Practice 

To identify given yeast culture (By performing various identification techniques studied in theory).To identify given mould culture (By performing various identification techniques studied in theory).To demonstrate dimorphism in fungi. To process clinical samples for laboratory diagnosis of fungal infections i.e. skin, hair, nail and body fluids.

Module III

Introduction to medical virology, Classification of viruses. Introduction to medically important viruses (Both DNA and RNA). Collection, transportation and storage of sample for viral diagnosis .Staining techniques used in Virology. Processing of samples for viral diagnosis (Egg inoculation and tissue culture). Antiviral drugs.

Practice

Demonstration of fertilized hen egg. Demonstration of various inoculation routes in fertilized hen egg.

Reference

Bailey & Scott’s Diagnostic Microbiology.

Ebook link- https://www.pdfdrive.com/bailey-scotts-diagnostic-microbiology-e187863782.html

Basic virology by Edward K. Wagner.

Ebook link-https://www.pdfdrive.com/basic-virology-e18900518.html

Essential in clinical microbiology by C A Kauffman and J D Sobel, 2^{nd Ed.}

Ebook link-https://www.pdfdrive.com/essentials-of-clinical-mycology-second-edition-e39564930.html

Guidelines on Establishment of Virology Laboratory by WHO.

Emanual link-https://apps.who.int/iris/rest/bitstreams/911047/retrieve

Online Tutorial Links

1. Antiviral drugs.

Elink-https://www.coursera.org/lecture/aids-fear-hope/05-07-the-drugs-dr-riddell-describes-antiretroviral-therapy-Zeb6F

2. Egg Inoculation and tissue culture methods.

Elink-https://vlab.amrita.edu/?sub=3&brch=76&sim=1223&cnt=2

Simulation Links for Lab

1. Demonstration of various routes in fertilized hen egg.

Elink-https://vlab.amrita.edu/?sub=3&brch=76&sim=1223&cnt=2

2. Identification of yeast culture by various identification technique.

Elink-https://vlab.amrita.edu/?sub=3&brch=76

3. To prepare culture media used routinely in mycology.

Elink-https://vlab.amrita.edu/?sub=3&brch=76&sim=693&cnt=2

Session Plan

Session 1

Lecture Topic-Introduction to medical mycology.

Lecture Video-https://www.youtube.com/watch?v=VVuYGkk_I8s

Lecture Notes-

https://jamanetwork.com/journals/jamadermatology/fullarticle/542184

Session 2

Lecture Topic-Basic concepts about superficial Mycoses.

Lecture video-

https://www.coursera.org/lecture/dermatology/fungal-infections-ZMX7J

Lecture Presentation-https://www.slideshare.net/rachnatewari/superficial-mycoses-79009092

Session 3

Lecture Topic-Basic concepts about deep Mycoses.

Lecture Video-https://www.coursera.org/lecture/stories-of-infection/fungal-infections-PposM

https://www.youtube.com/watch?v=qAjfOuTIWCI

Lecture Presentation-https://www.slideshare.net/doctortvrao/deep-fungal-infections

Session 4

Lecture Topic-Classification and general characteristics of various medically important fungi.

Lecture Video- https://www.coursera.org/lecture/antibiotic-stewardship/candidemia-63OUW

https://www.coursera.org/lecture/epidemic-pandemic-outbreak/week-2-video-1-what-causes-infection-what-is-an-infectious-disease-2385C

Lecture Notes-

https://www.coursera.org/lecture/hkuepidemics/video-2-2-emerging-infectious-diseases-at-the-human-animal-interface-onPdf

https://slideplayer.com/slide/6567469/

Session 5

Lecture Topic-Normal fungal flora.

Lecture Video-https://www.youtube.com/watch?v=JYRkXhT1XEs

Lecture Presentation- https://www.slideshare.net/doctorrao/normal-human-flora

Session 6

Lecture Topic-Morphological characteristics of common fungal laboratory contaminants.

Lecture Notes-https://nios.ac.in/media/documents/dmlt/Microbiology/Lesson-51.pdf

Session 7

Lecture Topic-Cultural characteristics of common fungal laboratory contaminants.

Lecture Video-https://vlab.amrita.edu/?sub=3&brch=76&sim=693&cnt=1

Session 8

Lecture Topic-Culture media used in mycology.

Lecture Video-https://vlab.amrita.edu/?sub=3&brch=76&sim=693&cnt=2

Lecture Presentation- https://www.slideshare.net/viveksanjumt/culture-of-fungus

Session 9

Lecture Topic-Fungal Staining.

Lecture Video- https://www.youtube.com/watch?v=OMIF1Elr9i4

https://www.youtube.com/watch?v=3ntWnf08P8Q

Lecture Presentation-https://microbeonline.com/lactophenol-cotton-blue-lpcb-mounts-principle-staining-protocol/#:~:text=Lactophenol%20cotton%20blue%20solution%20is,elements%20are%20stained%20intensely%20blue.

Session 10

Practice Topic- To prepare culture media used routinely in mycology.

Elink-https://vlab.amrita.edu/?sub=3&brch=76&sim=693&cnt=2

Session 11

Practice Topic- To perform all the staining techniques for identification of fungi.

Elink- https://www.youtube.com/watch?v=OMIF1Elr9i4

Session 12

Practice Topic- Microscopic and macroscopic characteristics of fungus.

Elink-https://www.youtube.com/watch?v=3ntWnf08P8Q

Session 13

Lecture Topic-Direct microscopy in Medical mycology laboratory.

Lecture Video-https://www.youtube.com/watch?v=sOLf5cDJPeI

Lecture Presentation-https://www.slideshare.net/NaveenParvathareddy/lab-diagnosis-of-fungal-infections

Session 14

Lecture Topic-Processing of clinical samples for diagnosis of fungal infections from Skin.

Lecture Video-https://www.coursera.org/lecture/dermatology/fungal-infections-ZMX7J

Lecture Presentation- https://www.slideshare.net/DineshJain4/laboratory-diagnosis-of-fungal-infections

Session 15

Lecture Topic-Processing of clinical samples for diagnosis of fungal infections from nail and hair.

Lecture Video-https://www.youtube.com/watch?v=GV840R4Pyi4

Lecture Notes-https://www.cdc.gov/fungal/features/fungal-infections.html

Session 16

Lecture Topic-Processing of clinical samples for diagnosis of fungal infections from-pus and sputum.

Lecture Video-https://www.youtube.com/watch?v=40BtYb_4zkE

Lecture Notes-https://www.brmc-cares.com/Uploads/Public/Documents/Division%202/Brownwood%20Regional%20Medical%20Center/New%20Lab%20Files/Fungus%20Culture%20Collection.pdf

Session 17

Lecture Topic-Processing of clinical samples for diagnosis of fungal infections from- CSF and other body fluids.

Lecture Video- https://www.youtube.com/watch?v=KSCFYLUJc7U

Lecture Presentation- https://www.slideshare.net/GauravShelgaonkar/csf-examination-53071410

Session 18

Lecture Topic-Techniques used for isolation and identification of medical important fungi.

Lecture Video-https://vlab.amrita.edu/?sub=3&brch=76

Lecture Presentation-http://www.journalarrb.com/index.php/ARRB/article/download/26618/49934/

Session 19

Lecture Topic-Techniques used for isolation and identification of medical important mould and yeast.

Lecture Video- https://vlab.amrita.edu/?sub=3&brch=76

Lecture Presentation-https://www.slideshare.net/SaifulIslam750/fungimolds-and-yeasts-86022186

Session 20

Practice Topic-Identify given yeast culture (By performing various identification technique)

Elink- https://vlab.amrita.edu/?sub=3&brch=76

Session 21

Practice Topic-Identify given mould culture (By performing various identification technique)

Elink- https://www.youtube.com/watch?v=GV840R4Pyi4

Session 22

Practice Topic- To demonstrate dimorphism in fungi.

Elink-https://www.youtube.com/watch?v=C8vwxm5GJwU

Session 23

Practice Topic- Processing of  skin samples for laboratory diagnosis of fungal infection.

Elink- https://www.youtube.com/watch?v=FohwEA5byYM

Session 24

Practice Topic-Processing of  nail samples for laboratory diagnosis of fungal infection.

Elink-https://www.youtube.com/watch?v=WyyjhA5gG7M

Session 25

Practice Topic- Processing of  hair samples for laboratory diagnosis of fungal infection.

Elink-https://www.youtube.com/watch?v=4J1T2mQMxXM

Session 26

Practice Topic-Processing of  body fluids samples for laboratory diagnosis of fungal infection.

Elink-https://www.youtube.com/watch?v=sOLf5cDJPeI

https://www.youtube.com/watch?v=SOiee63UaCs

Session 27

Practice Topic- Microscopic and macroscopic observation of fungi.

Elink-

https://www.youtube.com/watch?v=-uyBJ0nv4oI

https://www.youtube.com/watch?v=VuOUbEYkxdg

https://www.youtube.com/watch?v=rl2iQZ91mVg

Session 28

Lecture Topic-Introduction to medical virology.

Lecture Video - https://www.virology.ws/2013/12/03/virology-at-coursera/

Lecture Presentation-https://www.slideshare.net/doctorrao/medical-virology-introduction

Session 29

Lecture Topic- Classification of viruses.

Lecture Notes-https://www.coursera.org/lecture/aids-fear-hope/05-07-the-drugs-dr-riddell-describes-antiretroviral-therapy-Zeb6F

Session 30

Lecture Topic-Introduction to medically important DNA viruses.

Lecture Notes-https://www.coursera.org/lecture/stories-of-infection/hepatitis-b-77bsj

Lecture Presentation- https://www.slideshare.net/amanullah9803150/classification-of-virus

Session 31

Lecture Topic-Introduction to medically important RNA viruses.

Lecture Notes-https://www.coursera.org/learn/covid19clinicalupdate

http://apps.searo.who.int/pds_docs/B4416.pdf

Session 32

Lecture Topic-Collection of sample for viral diagnosis .

Lecture Video- https://www.youtube.com/watch?v=Jmhp9C15iK0

https://www.youtube.com/watch?v=syXd7kgLSN8

https://www.youtube.com/watch?v=ZJ4RzDGRnEI

Lecture Notes- https://apps.who.int/iris/rest/bitstreams/911047/retrieve

Pg. No- 27-32

Session 33

Lecture Topic-Transportation and storage of sample for viral diagnosis.

Lecture Video-https://www.youtube.com/watch?v=FatYUa_HNx8

Lecture Notes-https://apps.who.int/iris/rest/bitstreams/911047/retrieve

Pg. No.32-36

Session 34

Lecture Topic-Staining techniques used in Virology.

Lecture Notes-https://agrilife.org/vetmed/files/2012/10/LS_5_4_sample_lesson.pdf

Session 35

Lecture Topic-Processing of samples for viral diagnosis -Egg inoculation method.

Lecture Video- https://vlab.amrita.edu/?sub=3&brch=76&sim=1223&cnt=2

Lecture Presentation-https://www.slideshare.net/nomanalam186/egg-inoculation-technique-complete-1

Session 36

Lecture Topic-Processing of samples for viral diagnosis -  tissue culture method.

Lecture Video- https://www.youtube.com/watch?v=Yi13OAshhSk

Lecture Presentation-https://www.slideshare.net/vivekaiden/cultivation-of-virus-120166509

Session 37

Lecture Topic-Antiviral drugs.

Lecture Notes-https://www.coursera.org/lecture/aids-fear-hope/05-07-the-drugs-dr-riddell-describes-antiretroviral-therapy-Zeb6F

Session 38

Practice Topic- Demonstration of various roots of fertilized hen egg.

Practice video-https://vlab.amrita.edu/?sub=3&brch=76&sim=1223&cnt=2

Session 39

Practice Topic- Animal inoculation method.

Practice Video-

https://www.youtube.com/watch?v=41P3hJEa96w

Session 40

Practice Topic-Viral growth study.

Practice Video-

https://youtu.be/oIyF8PcOOes

Session 41

Revision  and discussion class.

Session 42

Revision and discussion class

Case Studies

Case Studies