Nano-pharmaceuticals and Bio-Medical science
Teacher
Dr. Srikanta Sahu
Category
Core Courses
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Course Reviews
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Course Name : Nano-pharmaceuticals and Bio-Medical science
Code(Credit) : CUTM1422(3-0-1)
Course Objectives
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To obtain an hands-on experience in converting a small novel idea / technique into a working model / prototype involving multi-disciplinary skills and / or knowledge and working in at team.
Course Outcome
| COs | Course outcomes | Mapping COs with POs (High-3, Medium-2, Low-1) |
| CO1 | Able to gain Knowledge on implementing chemistry, biology and nano-science to solve the biomedical problems. | PO1 (3) |
| CO2 | Research and problem Solving | PO2(3), PO3(2) |
| CO3 | Design and Development | PO3(3) |
| CO6 | Individual and Team work | PO6(3) |
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To conceptualize a novel idea / technique into a product or paper
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To think in terms of multi-disciplinary environment
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To understand the management techniques of implementing a project
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To take on the challenges of teamwork, prepare a presentation in a professional manner, and document all aspects of design work
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To bridge the gap between industry and academia and they can pursue their dream in either directions.
Course Syllabus
Module I (6) hours
Nanotechnology in Food and pharmaceutical industry: Nano particle based drug delivery systems, Regenerative medicine, nano-immuno conjugates, Bio-availability and delivery of nutraceuticals and functional foods using nanotechnology, Polymer-based nano-composites for food packaging, nano-composites for food packaging, Toxicity and environmental risks of nano-materials
Assignment 1: Nano-composites for food packaging, Toxicity and environmental risks of nano-materials
Module II (6) hours
Nanotechnology in Biomedical and Pharmaceutical Industry: Tissue engineering/regenerative medicine, Nano-robotics in surgery, Nano-tools for early detection diseases, Nano-medicine for cancer treatment
Assignment 2 : Tissue engineering/regenerative medicine
Module III (6) hours
Nanotechnology in Agriculture: Introduction of Nanotechnology in agriculture, Potential of nano-fertilizers, Precision farming, smart delivery system, Insecticides using nanotechnology
Assignment 3 : Smart delivery system
Module IV (6) hours
Molecular Cell Biology: Cell- Structure & Function of Cell Membrane, Different cell types and their Functions, Sub-cellular Organelles and their Functions, Nucleotide- Structure and Functions of DNA & RNA. Biologically important nucleotide, protein synthesis, unnatural amino acid, Mechanistic understanding of various diseases and target identification for early detection
Assignment 4: Cell culture techniques and Visualizing cell organelles using fluorescent probes
Module V (6) hours
Optical and non-optical probes: Introduction to Fluorescence, Designing of Organic and in-organic Fluorophores and study of photo-physical properties, Synthesis of Nano and bio-probe, study of sensing properties, Chemistry of Non-optical probe: PET and MRI probe
Assignment 5 : Uderstand the concept of fluorescence using laser light and Synthesis of Fluorescein dye
Module VI (6) hours
Protein Labeling and bio-medical applications: Affinity based protein labeling, Labeling of protein through unnatural amino acid, Diagnostic Probe for early detection of diseases and bio-analytes
Assignment 6: Fluorescent Labeling of proteins for Live Cell Imaging
Module VII (8) hours
Target Identification and Drug designing: High-Throughput Screenings, Affinity matrix approaches: On-bead affinity matrix, Biotin tags in affinity matrix, Fluorescent tags in affinity matrix, Photo-affinity tags in affinity matrix, Drug western approaches, Three-hybrid system approaches, Three-hybrid system approaches: Yeast three-hybrid systems, Mammalian three-hybrid systems, Phage display approaches, mRNA display approaches, Protein micro-array approaches, Drug affinity responsive target stability (DARTS)
Assignment 7 : A practical approach to antibody labeling
Books Recommended:
- Berg, J.M., Tymoczko, J. L. and Stryer, L. (2006) Biochemistry. VIth Edition. W.H. Freeman and Co.
- Nelson, D. L., Cox, M. M. and Lehninger, A. L. (2009) Principles of Biochemistry. IV Edition. W.H. Freeman and Co.
- Murray, R. K., Granner, D. K., Mayes, P. A. and Rodwell, V.W. (2009) Harper’s Illustrated Biochemistry. XXVIII edition. Lange Medical Books/ McGraw-Hill.
Session Plan
Session 1 (1 hour) Module-I
Nanotechnology in Food and pharmaceutical industry, Nano particle based drug delivery systems
https://www.youtube.com/watch?v=aFU5Qx-cLu8
https://www.youtube.com/watch?v=emEua2eJp1U
References:
Session 2 (1 hour)
Regenerative medicine, nano-immuno conjugates
https://www.youtube.com/watch?v=MdM0afV7bn4
https://www.youtube.com/watch?v=7Q3S6q97FiU
https://www.youtube.com/watch?v=tcPpBNwkU54&t=3s
References:
https://nanomedicine.mit.edu/nanotechnology/cancer-nanomedicine
Session 3 (1 hour)
Bio-availability and delivery of nutraceuticals and functional foods using nanotechnology
https://library.wur.nl/WebQuery/file/cogem/cogem_t45017dfd_001.pdf
https://www.teknoscienze.com/Contents/Riviste/PDF/AF4_2013_RGB_22-25.pdf
https://analyticalscience.wiley.com/do/10.1002/gitlab.906/full/
Session 4 (1 hour)
Polymer-based nano-composites for food packaging
https://www.youtube.com/watch?v=K__Hi-AmEVg
Reference:
Session 5 (2 hour)
Assignment 1: Nano-composites for food packaging, Toxicity and environmental risks of nano-materials
Session 6 (1 hour), Module II
Nanotechnology in Biomedical and Pharmaceutical Industry
https://www.youtube.com/watch?v=Q3Nu225bZoc
References:
https://nanomedicine.mit.edu/nanotechnology/cancer-nanomedicine
Session 7 (1 hour), Module II
Nano-robotics in surgery
https://www.youtube.com/watch?v=ayteOA5VDRI
References:
https://nanomedicine.mit.edu/nanotechnology/cancer-nanomedicine
Session 8 (1 hour), Module II
Session 9 (1 hour), Module II
Session 10 (1 hour), Module II
Assignment 2 : Tissue engineering/regenerative medicine
https://www.youtube.com/watch?v=7Q3S6q97FiU
References:
https://nanomedicine.mit.edu/nanotechnology/cancer-nanomedicine
Session 11 (1 hour), Module III
Introduction of Nanotechnology in agriculture
https://www.youtube.com/watch?v=hdDBvC7kop8
Reference:
Session 12 (1 hour), Module III
Potential of nano-fertilizers
https://www.youtube.com/watch?v=GDe0XxRtx0Q
References:
Session 13 (1 hour), Module III
Session 14 (1 hour), Module III
Insecticides using nanotechnology
Session 15 (2 hour), Module III
Assignment 3 : Smart delivery system
Session 16 (1 hour), Module IV
Molecular Cell Biology, Cell- Structure & Function of Cell Membrane, Different cell types and their Functions
Session 17 (1 hour), Module IV
Sub-cellular Organelles and their Functions
Session 18 (1 hour), Module IV
Mechanistic understanding of various diseases and target identification for early detection
https://www.coursera.org/learn/cancer/lecture/XsshR/incidence-and-etiology-of-cancer
Reference:
Session 19 (1 hour), Module IV
Nucleotide- Structure and Functions of DNA & RNA. Biologically important nucleotide, protein synthesis, unnatural amino acid
https://www.youtube.com/watch?v=gG7uCskUOrA&list=RDQM1TXXAdELXtU&start_radio=1
Session 20 (2 hour), Module IV
Assignment 4: Cell culture techniques and Visualizing cell organelles using fluorescent probes
References:
https://www.youtube.com/watch?v=o35ZQq0Uvzs&list=PL5Aj2t2VGlCGfOzyMScFOZvbY_AI2mO0f
https://www.youtube.com/watch?v=nr1tV_LuqJk
Session 21 (1 hour), Module V
Optical and non-optical probes, Introduction to Fluorescence
https://www.youtube.com/watch?v=W9p6Y8IW89A
Reference:
Session 22 (1 hour), Module V
Designing of Organic and in-organic Fluorophores and study of photo-physical properties
https://www.youtube.com/watch?v=Ok__iaEx_Hc
Reference:
Session 23 (1 hour), Module V
Synthesis of Nano and bio-probe, study of sensing properties
https://www.youtube.com/watch?v=G-MHYyWgmV0
https://www.youtube.com/watch?v=xDZP-p-_Ibw
Reference:
Session 24 (1 hour), Module V
Chemistry of Non-optical probe : PET and MRI probe
https://www.youtube.com/watch?v=k2jnSmpHqzg
https://www.youtube.com/watch?v=oySvkmezdo0
https://www.youtube.com/watch?v=E44W54z_Ykw
References:
Session 25 (2 hour), Module V
Assignment 5 : Uderstand the concept of fluorescence using laser light and Synthesis of Fluorescein dye
References:
https://www.youtube.com/watch?v=CcssdJf0pKQ
Session 26 (1 hour), Module VI
Protein Labeling strategy and development of probe for bio-medical application, Bio-Orthogonal chemistry for Protein labeling
References:
Session 27 (1 hour), Module VI
Affinity based protein labeling
https://www.youtube.com/watch?v=AL764rkcZSo
References:
Session 28 (1 hour), Module VI
Labeling of protein through unnatural amino acid
Session 29 (1 hour), Module VI
Diagnostic Probe for early detection of diseases and bio-analytes
https://www.youtube.com/watch?v=ZlMwUYWnBCA
https://www.slideshare.net/namarta28/factors-affecting-enzyme-activity-92404667
Session 30 (2 hour), Module VI
Assignment 6: Fluorescent Labeling of proteins for Live Cell Imaging
Session 31 (1 hour), Module VII
Target Identification and Drug designing, High-Throughput Screenings
Session 32 (1 hour), Module VII
Affinity matrix approaches: On-bead affinity matrix, Biotin tags in affinity matrix
Session 33 (1 hour), Module VII
Fluorescent tags in affinity matrix, Photo-affinity tags in affinity matrix
References:
Session 34 (1 hour), Module VII
Drug western approaches, Three-hybrid system approaches
Session 35 (1 hour), Module VII
Three-hybrid system approaches: Yeast three-hybrid systems, Mammalian three-hybrid systems
References:
Session 36 (1 hour), Module VII
Phage display approaches, mRNA display approaches, Protein micro-array approaches, Drug affinity responsive target stability (DARTS)
References:
https://www.youtube.com/watch?v=JjbOBbr4q0s
Session 37 (1 hour), Module VII
Phage display approaches, mRNA display approaches, Protein micro-array approaches, Drug affinity responsive target stability (DARTS)
References:
https://www.youtube.com/watch?v=JjbOBbr4q0s
Session 38 (2 hour), Module VII
Assignment 7: A practical approach to antibody labeling
https://www.youtube.com/watch?v=kUnBoOmfh1o
References:
Case Studies
Case Studies
Our Main Teachers
Srikanta Sahu completed his Ph.D. from IIT Delhi in Bio-organic chemistry under the supervision of Prof. V. Haridas. After his PhD, he moved for postdoctoral work at National University of Singapore (NUS) in the field of Bio-medical science and chemical biology under the guidance of Prof. Young Tae Chang. Later, he joined as a core […]