OCULAR MICROBIOLOGY & PATHOLOGY. CUTM -1789

Teacher

Syamala Alana Teja

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COURSE OBJECTIVES

This course covers the basic biological characteristics of pathogenic organisms
Biochemical
Pathogenic characteristics of the various microorganisms

COURSE OUTCOMES

T prepare the students to gain essential knowledge about the characteristics of bacteria ,viruses, fungi and parasites .
To acquire knowledge of the principle of sterilisation and disinfection in hospital and ophthalmic practice
To understand the pathogenesis of the disease caused by the organisms in the human body with particular reference to the eye infections
To understand the basic principles of diagnostic ocular Microbiology

course syllabus :

Module-1

Introduction to Microbiology :Types of Microorganisms, Physiology of Microorganisms –
Nutrition, Enzymes, Metabolism and energy, Microbial Growth ,Sterilization and disinfection
in the laboratory ,Control of Microbial Growth – Antimicrobial methods and Chemotherapy,
Microbes versus Humans- The development of Infection, the disease process, pathogenicity
and virulence.
Practice: Handling of microscope

Module-2

General Pathology : Principles ,Pathophysiology of Ocular Angiogenesis , Ocular Infections,
Pathology of cornea and Conjunctiva , Pathology of Uvea, Glaucoma Retina

Module-3

Ocular Bacteriology-Gram positive,(Staphylococcus auras, Staphylococcus epidermis,
Streptococcus, propionic bacterium, actinomycesNocardia) Bacteria including acid fast bacilli,

Mycobacterium tuberculosis, Mycobacterium Leprae)

Practice: Gram staining procedures

Module-4

Ocular Bacteriology - Gram negative Bacteria (pseudomonas, haemophiilus, Brucella,
Neisseria, Moraxella) .

Module-5

Pathology of retina in systemic disease/disorders , Pathology of eyelids and adnexa ,Pathology
of orbital space occupying lesions
Practice: Slit-lamp Examination

Module-6

Spirochetes (Treponema, Leptospiraceae),Virology: Classification of Viruses in Ocular
Disease, Rubella, Adenovirus, Oncogenic Viruses (HPV, HBV, EBV, Retroviruses), HIV. ,
Fungi : Yeasts, Filamentous, Dimorphic ,Intracellular parasites - Chlamydia, Protozoa
(Taxoplasmosis, Acanthamoeba,) Helminths ( Toxocariasis , Filariasis , Onchocerciasis ,
Trematodes )
Module-7

Pathology of the optic nerve, Retinoblastoma,Pathology of Lens .

Text Books:

1. Microbiology : M J Pelczaretal., 1999
2. BURTON G.R.W : Microbiology for the Health Sciences, St. Louis, J.P.
Lippincott Co.,3 rd Edn.,1988..
3. Pathology : CORTON KUMAR AND ROBINS (V EDITION) Pathological Basis
of the Disease, 2004.
4. Pathology of the eye & orbit : K S Ratnagar, 1997

Reference Books:
1. MACKIE &Mc CARTNEY Practical Medical Microbiology
2. SYDNEY M. FINEGOLD & ELLEN JO BARON :Diagnostic Microbiology (DM)

SESSION 1

Introduction to Microbiology : Types of Microorganisms
Physiology of Microorganisms-Nutrition ,Enzymes ,Metabolism and Energy

. Presentation B OM & P- PHYSIOLOGY OF MICROORGANISMS

Physiology of Microorganisms –Nutrition, Enzymes, Metabolism and energy:

https://www.youtube.com/watch?v=H3xIOvoevU4

https://www.youtube.com/watch?v=W5soSpVWMT8

Introduction to Microbiology :

Types of Microorganisms:

https://www.youtube.com/watch?v=ei6Z7orCpPk

https://www.youtube.com/watch?v=fU0XO1X1tAE&t=17s

https://www.slideshare.net/RuchikaMaurya4/general-and-ocular-microbiologypptx

SESSION 2

Microbial Growth
Control of Microbial Growth - Antimicrobial Methods and Chemotherapy

Microbial Growth:

https://www.youtube.com/watch?v=JIZ7lj3y4MQ&t=16s

https://www.youtube.com/watch?v=DfA-MHgi2ws

Presentation

https://www.slideshare.net/vinayawarad/growth-of-bacteria-172092302

SESSION 3

Microbes versus humans - Development of infection
Disease Process

Microbes versus Humans- The development of Infection, the disease process, pathogenicity and virulence.

https://www.youtube.com/watch?v=pMH0nNITX3U

Presentation

https://www.slideshare.net/jinx11/microbe-human-interaction

SESSION 4

Pathogenicity and Virulence of the Micro-organisms
The development of Infection, the disease process, pathogenicity and virulence. https://www.youtube.com/watch?v=pMH0nNITX3U

SESSION 5

General Pathology
Principles ,Pathophysiology of the Angiogenesis

Principles:

https://www.youtube.com/watch?v=1vaEVcMfa1E

Pathophysiology of Ocular Angiogenesis:

https://www.youtube.com/watch?v=Ep_nCSEDeAE

https://www.youtube.com/watch?v=95n4uIS06Qc

SESSION 6

Ocular Infections

Ocular Infections:Pathology of cornea and Conjunctiva :

https://www.youtube.com/watch?v=ydKbESsC3es

https://www.youtube.com/watch?v=Mtk8ooQPI9U

SESSION 7

Pathology of Uvea and its characteristics

Pathology of Uvea:

https://www.youtube.com/watch?v=BCkak69A2kE

SESSION 8

Pathology of Glaucoma /retina with different features
Glaucoma Retina: https://www.youtube.com/watch?v=hASPt3hksbA https://www.youtube.com/watch?v=TgjdPgSxeYg

SESSION 9

Pathology of Retina with its relevant differential features
Glaucoma Retina: https://www.youtube.com/watch?v=hASPt3hksbA https://www.youtube.com/watch?v=TgjdPgSxeYg

SESSION 10

Ocular Bacteriology introduction and differentiation

Ocular Bacteriology-Gram positive:

https://www.youtube.com/watch?v=AZS2wb7pMo4

SESSION 11

Gram Positive Bacteria example : Staphylococcus auras
Staphylococcus epidermis

Staphylococcus auras, Staphylococcus epidermis:

https://www.youtube.com/watch?v=0D2mKmUvxPc

SESSION 12

Streptococcus
Propionic bacterium

Streptococcus:

https://www.youtube.com/watch?v=HAtu6uNUZyY

propionic bacterium:

https://www.youtube.com/watch?v=-lfKbjDctF0

SESSION 13

Bacteria including acid fast Bacilli - with the staining procedure and identification .

Bacteria including acid fast bacilli: Mycobacterium tuberculosis

https://www.youtube.com/watch?v=jyTekFxyVeY

SESSION 14

Mycobacterium tuberculosis structure and characteristics

Bacteria including acid fast bacilli: Mycobacterium tuberculosis

https://www.youtube.com/watch?v=jyTekFxyVeY

SESSION 15

Mycobacterium leprae structure and characteristics

Myobacterium leprae:

https://www.youtube.com/watch?v=oXxWvHCdx7E

SESSION 16

Gram negative bacteria- Pseudomonas , Haemophilus

Ocular Bacteriology - Gram negative Bacteria

https://www.youtube.com/watch?v=NWASSXDzHRs

haemophiilus

https://www.youtube.com/watch?v=ug-M1nIhfIA

pseudomonas,

https://www.youtube.com/watch?v=Dv6J-8Vi2t4

SESSION 17

Gram negative bacteria- Brucella
Neisseria
Moraxella
Brucella https://www.youtube.com/watch?v=pixZUrdn-wY Neisseria https://www.youtube.com/watch?v=OayQEFV0vJA

SESSION 18

Pathology of Retina in systemic diseases/disorders

Pathology of retina in systemic disease/disorders

https://www.youtube.com/watch?v=HHefZIQzj8Y

SESSION 19

Pathology of Eyelids

SESSION 20

Pathology of Adnexa

SESSION 21

Pathology of orbital space occupying lesions

Pathology of orbital space occupying lesions

https://www.youtube.com/watch?v=HKEA4p5k66U

SESSION 22

Spirochetes ( Treponema ,Leptospiraceae)

Spirochetes (Treponema, Leptospiraceae),

https://www.youtube.com/watch?v=XG1gEZD3_L8

SESSION 23

Virology : Classification of Viruses in ocular Diseases