Course Name : Parasitology

Course (Credit) : CUTM1748(3-2-0)

Course Objectives

Describe basic morphology, life cycle, pathogenesis, lab diagnosis and treatment of parasites (Protozoa, metazoa and Helminth)

Perform appropriate laboratory techniques used in the processing of specimens and identification of parasites.

Perform sample collection , transportation and preservation of sample.

Course outcome

• COs	Course Outcomes	Mapping COs with POs (High-3, Medium-2, Low- 1)
  CO1	Understand the general characteristics and classification of protozoa and helminths. Learn the methods of collection, transport, processing, and preservation of samples for parasitological investigations.	PO1(3)
  CO2	Acquire knowledge about the morphology, life cycle, and laboratory diagnosis of specific parasites,	PO1(3), PO2(3)
  CO3	Gain knowledge about laboratory diagnosis methods for various parasites, including direct and indirect methods, rapid diagnostic tests (RDTs), Quantitative Buffy Coat Assay (QBC), Malaria Rapid Diagnostic Test (MRDT), and Leishmanin test.	PO1(3)

          Course Outline

• Module-I
  Introduction to Medical Parasitology, General characteristics and classification of protozoa and helminthes. Collection, Transport, processing and preservation of samples for routine parasitological investigations.Practice: Method of sample Collection, Transport, processing and preservation of samples for routine parasitological investigations.

  Module II

   

  Morphology, life cycle and lab diagnosis of Giardia and Entamoeba, T.solium, T.saginata, malaria parasite with special reference to P. vivax and P. falciparum and Leishmania donovani (Kala azar). Morphology, life cycle and lab diagnosis of hook worm and round worm ( Ascaris lumbricoides and Anchylostoma duodenale ).Most common symptoms of parasitic infection and diagnosis and treatment methods. Procedures used in microscopic examination of stool from a parasitic infected individual.

  Practice: Routine Stool examination for detection of intestinal parasites. Concentration techniques for demonstration of Ova (Principles and applications). Identification of adult worms from model’s or slide’s method.

   

  Module III

   

  Laboratory Diagnosis of Various Parasites: Direct method, Indirect method. Rapid Diagnostic Tests (RDTs), Quantitative buffy coat assay (QBC), Malaria Rapid diagnostic test (MRDT), Leishmanin test.

  Practice: Demonstration of some rapid diagnostic method used for parasite identification.

   

  Suggested Readings:

  1. Textbook of medical Parasitology. (e-book link-https://www.pdfdrive.com/textbook-of- medical-parasitology-textbook-of-medical-parasitology-e128716897.html

  Parasitology    book    by        K.D.    Chatterjee.      (e-book            link- https://sites.google.com/site/bkthrtrpazg/atahrgiwu.https://www.goodreads.com/book/show/243 66965-parasitology-protozoology-and- helminthology-with-two-hundred-fourteen

  Ebook Link- https://www.pdfdrive.com/textbook-of-medical-parasitology-textbook-of-medical-parasitology-e128716897.html

  Online Tutorial Links

  Stool Examination.

  ELink- https://www.youtube.com/watch?v=-iI2PxmHxuo

  https://www.youtube.com/watch?v=MRzUXg8kFiY

  https://www.youtube.com/watch?v=_ePqcdDKCe0

  Malaria thick smear preparation.

  ELink- https://www.youtube.com/watch?v=WPP7AjmStBg

  Malaria thin smear preparation.

  ELink- https://www.youtube.com/watch?v=acoALifVvb8

   

   

  Session Plan

  Session 1

  Lecture Topic- Laboratory Organization (Parasitology Lab)

  Lecture Video- https://www.youtube.com/watch?v=U8mLe8xh5yM

  Lecture Notes-http://whqlibdoc.who.int/publications/9241544104_(part1).pdf?ua=1

• https://www.slideshare.net/MosabNouraldein/introduction-to-medical-parasitology-98787516
• https://www.google.com/imgres?imgurl=https://img.brainkart.com/imagebk42/nvwWq3z.jpg&imgrefurl=https://www.brainkart.com/article/Parasite-and-Host_41024/&h=923&w=657&tbnid=Xmqa0SuJeOWW1M&tbnh=266&tbnw=189&usg=AI4_-kRQAmKI8S_y-XGPecZ_KYc3p6w6yw&vet=1&docid=D8EyqP4VqwtpdM
• Session 2Lecture Topic-Selection, Collection, Perseveration and Transportation of Samples.Lecture Video- https://www.youtube.com/watch?v=heTQcEMDSGULecture Notes- http://whqlibdoc.who.int/publications/9241544104_(part1).pdf?ua=1Session 3Practice Topic- Safety measures in parasitology lab and laboratory organization.

  Practice Video- https://www.youtube.com/watch?v=U8mLe8xh5yM

  Session 4

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Entamoeba histolytica.

  Lecture Video- https://www.youtube.com/watch?v=Klx0uDfCIRA

  Lecture Presentation-https://www.slideshare.net/RaNaMB1/entamoeba-histolytica-95813165

  Lecture Readings- Parasitology by K D Chatterjee(13^th Ed), pg.18-41-chapter II.

  Session 5

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Giardia lamblia.

  Lecture Video- https://www.youtube.com/watch?v=cpu9PRIEYWY

  Lecture Presentation- https://www.slideshare.net/azizkhan1995/giardia-lamblia-63763763

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.47-48-chapter II

  Session 6

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Trichomonas spp.

  Lecture Video- https://www.youtube.com/watch?v=5t6sHgRqavw

  Lecture Presentation- https://www.slideshare.net/PakRose1/trichomonas-31612943

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.49-51-chapter II

  Session 7

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Cyrptosporidium.

  Lecture Video- https://www.youtube.com/watch?v=RIFVlIrd8L0

  Lecture Presentation- https://www.slideshare.net/alisaqlain111/cryptosporidium-39089694

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.134-chapter III

  Session 8

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Cyclospora.

  Lecture Video- https://www.youtube.com/watch?v=dVFRgmZpTfQ

  Lecture Notes-  https://www.cdc.gov/parasites/cyclosporiasis/index.html

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.135-chapter III

  Session 9

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Isospora.

  Lecture Video- https://www.youtube.com/watch?v=CyMvK1dumb8

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.128-chapter III

  Session 10

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Ascaries lumbricoides.

  Lecture Video- https://www.youtube.com/watch?v=4U3bIXaosh0

  https://youtu.be/UIXZYF1dZQ4

  Lecture Presentation- https://www.slideshare.net/sakuramarnati/ascaris-lumbricoides-14172492

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.229-232-chapter VIII

  Session 11

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Hook worm: Anacyclostoma.

  Lecture Video- https://www.youtube.com/watch?v=QIWc-55IQzk

  https://www.youtube.com/watch?v=HXAiDBiZ7FA

  Lecture Presentation- https://www.slideshare.net/RaNaMB1/ancylostoma-duodenale-hookwormi2015

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.212-220-chapter VII

  Session 12

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Enterobius vermicularis.

  Lecture Presentation- https://www.slideshare.net/drmoiz/enterobius-vermicularispinworm

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.225-chapter VII

  Session 13

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Trichuris trichuira.

  Lecture Video- https://www.youtube.com/watch?v=ZDIHQ_FG_68

  Lecture Presentation- https://www.slideshare.net/anady28/trichuris-trichiura-11658065

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.205-206-chapter VII

  Session 14

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Strongloides spp.

  Lecture Video- https://www.youtube.com/watch?v=5MuXsdIZSEc

  Lecture Presentation- https://www.slideshare.net/MizanRahman9/strongyloides-stercoralis-63214733

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.208-212chapter VII

  Session 15

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Taenia spp.

  Lecture Video- https://www.youtube.com/watch?v=kNEYwXi-5_4

  Lecture Presentation- https://www.slideshare.net/doctorrao/tinea-solium

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.151-chapter VI

  Session 16

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Echinococcus spp.

  Lecture Video- https://www.youtube.com/watch?v=_rdb5j7jDhQ

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.165-chapter VI

  Session 17

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Malaria (Plasmodium) spp.

  Lecture Video- https://www.youtube.com/watch?v=1v55yg0RfoY

  Lecture Presentation- https://www.slideshare.net/AbhishekJoshi151/malaria-72552248

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.90-102-chapter III

  Session 18

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Leishmania spp.

  Lecture Video- https://www.youtube.com/watch?v=wzjSmxThgJ0

  Lecture Notes- https://www.who.int/news-room/fact-sheets/detail/leishmaniasis

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.64-68chapter II

  Session 19

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Wuchereria spp.

  Lecture Video- https://www.youtube.com/watch?v=9zMdg9PFCW0

  Lecture Presentation- https://www.slideshare.net/alubajessabeth/wuchereria-bancrofti-39245224

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.238-247chapter VIII

  Session 20

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Oncocerca.

  Lecture Presentation- https://www.slideshare.net/luvmatyns/onchocerciasis

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.249-251-chapter VIII

  Session 21

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Dracuhculus.

  Lecture Presentation- https://www.slideshare.net/don.clark/dracunculiasis-dracunculus-medinensis-guinea-worm-disease

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.254-258chapter VII

  Session 22

  Lecture Topic- Habitat, morphology, life cycle, pathogenesis, lab diagnosis and treatment of Paragonimus westermani.

  Lecture Video- https://www.youtube.com/watch?v=en1bKReS0bA

  Lecture Presentation- https://www.slideshare.net/amin7ma/paragonimiasis-lung-fluke-disease

  Lecture Readings-Parasitology by K D Chatterjee(13^th Ed), pg.196-198 chapter VII

  Session 23

  Practice Topic- Examination and observation of Stool.

  Practice Video- https://www.youtube.com/watch?v=MRzUXg8kFiY

  https://www.youtube.com/watch?v=-iI2PxmHxuo

  https://www.youtube.com/watch?v=_ePqcdDKCe0

  Session 24

  Lecture Topic- Laboratory diagnosis method (Direct method).

  Lecture Video- https://www.youtube.com/watch?v=r69QmZQc0Qc

  https://www.youtube.com/watch?v=-iI2PxmHxuo

  Lecture Presentation- https://www.slideshare.net/Prbn/lab-dia-of-parasite

  Session 25

  Lecture Topic- Laboratory diagnosis method (Indirect Method)

  Lecture Video- https://www.youtube.com/watch?v=WTelVO4ywUc

  Lecture Presentation- https://www.slideshare.net/Prbn/lab-dia-of-parasite

  Session 26

  Lecture Topic- Laboratory diagnosis method (Rapid Methods)

  Lecture Video- https://www.youtube.com/watch?v=hft79S__C_8

  Lecture Notes- https://www.oie.int/doc/ged/D8270.PDF

  Session 27

  Lecture Topic- Laboratory diagnosis method (Molecular Technique)

  Lecture Video- https://www.youtube.com/watch?v=nbYGjO5fDA8

  Lecture Notes- https://www.hindawi.com/journals/ipid/2009/278246/

  Session 29

  Lecture Topic- Parasite Culture methods.

  Lecture Video- https://www.youtube.com/watch?v=1LdYQ5Utu3Y

  Lecture Presentation- https://www.slideshare.net/prdiphamal/parasite-culture

  Session 30

  Lecture Topic- Different Stains used in Diagnostic Parasitology.

  Lecture video- https://www.youtube.com/watch?v=acoALifVvb8

  https://www.youtube.com/watch?v=WPP7AjmStBg

  Lecture Presentation- https://www.slideshare.net/doctortvrao/parasitology-basic-identification-methods

  Session 31

  Practice Topic- Identification of parasite and morphology study by slides study. ( Malaria Thick smear preparation)

  Practice Video- https://www.youtube.com/watch?v=WPP7AjmStBg

  Session 32

  Practice Topic- Malaria Thin smear Preparation.

  Practice Video- https://www.youtube.com/watch?v=acoALifVvb8