PHARMACEUTICAL BIOTECHNOLOGY (Theory)

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SUMAN KUMAR MEKAP

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Course Name : PHARMACEUTICAL BIOTECHNOLOGY (Theory)

Code(Credit) : BPHT3205 (4-0-0)

Course Objectives

Biotechnology has a long promise to revolutionize the biological sciences and technology.

Scientific application of biotechnology in the field of genetic engineering, medicine and fermentation technology makes the subject interesting.
Biotechnology is leading to new biological revolutions in diagnosis, prevention and cure of diseases, new and cheaper pharmaceutical drugs.
Biotechnology has already produced transgenic crops and animals and the future promises lot more.

Course Outcomes

CO	Statements	Mapping with POs
CO-1	Understanding the importance of Immobilized enzymes in Pharmaceutical Industries	PO1, PO7, PO11
CO-2	Genetic engineering applications in relation to production of pharmaceuticals	PO1, PO2, PO5, PO7
CO-3	Importance of Monoclonal antibodies in Industries	PO1, PO5, PO6, PO10
CO-4	To imparts a comprehension of advanced skills necessary for developing novelty work in the field of biotechnology	PO1, PO5, PO7, PO10
CO-5	Able to apply the concepts of Immunology, Enzyme Immobilization, Microbial Transformation to Prepare Vaccines, Diagnostic Agents, Enzymes and fermentation technology	PO1, PO5, PO10, PO11

Course Syllabus

Unit I: Brief introduction to Biotechnology
a) Biotechnology with reference to Pharmaceutical Sciences.
b) Enzyme Biotechnology- Methods of enzyme immobilization and applications.
c) Biosensors- Working and applications of biosensors in Pharmaceutical Industries.
d) Brief introduction to Protein Engineering.
e) Use of microbes in industry. Production of Enzymes- General consideration - Amylase, Catalase, Peroxidase, Lipase, Protease, Penicillinase.
f) Basic principles of genetic engineering.

Unit II: DNA technology
a) Study of cloning vectors, restriction endonucleases and DNA ligase.
b) Recombinant DNA technology. Application of genetic engineering in medicine.
c) Application of r DNA technology and genetic engineering in the production of: i) Interferon ii) Vaccines- hepatitis- B iii) Hormones-Insulin.
d) Brief introduction to PCR

Unit III: Immunity
Types of immunity- humoral immunity, cellular immunity
a) Structure of Immunoglobulins
b) Structure and Function of MHC
c) Hypersensitivity reactions, Immune stimulation and Immune suppressions.
d) General method of the preparation of bacterial vaccines, toxoids, viral vaccine, antitoxins, serum-immune blood derivatives and other products relative to immunity.
e) Storage conditions and stability of official vaccines
f) Hybridoma technology- Production, Purification and Applications
g) Blood products and Plasma Substitutes.

Unit IV Immunological techniques
a) Immuno blotting techniques- ELISA, Western blotting, Southern blotting.
b) Genetic organization of Eukaryotes and Prokaryotes
c) Microbial genetics including transformation, transduction, conjugation, plasmids and transposons.
d) Introduction to Microbial biotransformation and applications.
e) Mutation: Types of mutation/mutants.

Unit V: Fermentation
a) Fermentation methods and general requirements, study of media, equipments, sterilization methods, aeration process, stirring.
b) Large scale production fermenter design and its various controls.
c) Study of the production of - penicillins, citric acid, Vitamin B12, Glutamic acid, Griseofulvin
d) Blood Products: Collection, Processing and Storage of whole human blood, dried human plasma, plasma Substitutes.

Recommended Books :
1. B.R. Glick and J.J. Pasternak: Molecular Biotechnology: Principles and Applications of Recombinant DNA: ASM Press Washington D.C.
2. RA Goldshy et. al., : Kuby Immunology.
3. J.W. Goding: Monoclonal Antibodies.
4. J.M. Walker and E.B. Gingold: Molecular Biology and Biotechnology by Royal Society of Chemistry.
5. Zaborsky: Immobilized Enzymes, CRC Press, Degraland, Ohio.
6. S.B. Primrose: Molecular Biotechnology (Second Edition) Blackwell Scientific Publication.