Pharmaceutical Microbiology
Teacher
Dr. Soumya Jal
Category
Core Courses
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Course Reviews
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Course Name : Pharmaceutical Microbiology
Code(Credit) : CUTM1739 (3-1-0)
Course Objectives
- Understanding of types & synthesis of antimicrobial agents
- Manufacture of antibiotics
- To understand the mechanism of action of antibiotics
- To study how microorganisms are known to develop resistance to antibiotics
Learning Outcomes
- With the completion of the course, the students will acquire detailed knowledge of antimicrobial agents, their mechanism of action and basis of resistance of microbes to these antimicrobials, formulations.
- They will develop an understanding of different types of disinfectants/antiseptics and their uses, evaluation of their bactericidal and bacteriostatic action.
- The conceptual understanding of the subject provides opportunities for employbility and scopes for higher education.
Course Syllabus
Module 1
Pharmaceutical Microbiology: Chemical disinfectants, Antiseptic, Antibiotics, static and cidal activity. Types of Antibiotics. Synthetic Antimicrobial Agents, Antifungal drug, Antiviral drug.
Module 2
Manufacture of Antibiotics: Production of penicillin, Production of Streptomycin.
Assessment of New Antibiotics: Parameters for determination of the usefulness of antibiotics (in vitro and in vivo).
Pharmacokinetics and Pharmacodynamics of Antimicrobial agents.
Antibiotic Assay: Microbiological methods: Disc diffusion technique, Dilution technique.
Microbial Spoilage and Preservation of Pharmaceutical Products.
Module 3
Mechanisms of Action of Antibiotics: Cell wall synthesis inhibitors, Inhibitors of protein biosynthesis, Inhibitors of tetrahydrofolate, Disorganize the cytoplasm membrane. Mode of action of Antibiotics (Cell wall synthesis inhibitors, Inhibitors of protein biosynthesis, Inhibitors of tetrahydrofolate, Disorganize the cytoplasm membrane). Bacterial Resistance to Antibiotics. Biochemical mechanisms of resistance, Genetic basis of antibiotic resistance. Problems in antibiotic therapy due to resistance.
Suggested Readings:
- W B Hugo and A D Russel, Pharmaceutical Microbiology, 2nd Edition
(e-Book link: https://www.pdfdrive.com/hugo-and-russells-pharmaceutical-microbiology-e34745384.html )
- T H Sandal Pharmaceutical Microbiology: Essentials for quality assurance and quality control. Woodhead Publishing Series.
(e-Book link: https://www.pdfdrive.com/pharmaceutical-microbiology-essentials-for-quality-assurance-and-quality-control-e157918748.html )
- Willey MJ, Sherwood LM & Woolverton C J. (2013). Prescott, Harley and Klein’s Microbiology. 7thEd., McGraw Hill.
(e-Book link: https://www.pdfdrive.com/prescott-harley-and-kleins-microbiology-7th-ed-e188166539.html )
Online tutorial links:
- Lecturio link
(https://app.lecturio.com/#/course/s/6956 )
Simulation links for labs:
All the practice components can be performed by simulation as well as virtual labs.
Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).
labsforlife (http://labsforlife.in/)
Online Source: Lecturio, Udemy, Coursera, article library, NCBI, Study.com, You tube, unacademy, academia
Session Plan
Session 1
Topic: Chemical disinfectants
Video on Chemical disinfectants
https://www.youtube.com/watch?v=_8MouGg-sr0
https://www.youtube.com/watch?v=U08CFAJXKW0
https://www.youtube.com/watch?v=wzQ8NJg98lo
https://www.youtube.com/watch?v=ujG6hSk7eEk
Slideshare link to Chemical disinfectants
Session 2
Topic: Antiseptic
Video on Antiseptic
https://www.youtube.com/watch?v=wzQ8NJg98lo
Slideshare link to Antiseptic
https://www.slideshare.net/nasertadvi/antiseptics-and-disinfectants-15555738
https://www.slideshare.net/arkam741/antiseptic-and-disinfectant-88221452
Session 3
Topic: Antibiotics
Video on Antibiotics
https://www.youtube.com/watch?v=k8xat-XzgEA
Slideshare link to Antibiotics
https://www.slideshare.net/phmosarrof/presentation-on-antibiotics
Session 4
Topic: static and cidal activity
Video on static and cidal activity
Session 5
Topic: Types of Antibiotics
Video on Types of Antibiotics
Session 6
Topic: Synthetic Antimicrobial Agents
Video on Synthetic Antimicrobial Agents
https://www.youtube.com/watch?v=k8xat-XzgEA
Slideshare link to Antimicrobial Agents
https://www.slideshare.net/minhazahmed21/min-antibac
https://www.slideshare.net/ShariqHabib/antimicrobial-agents-79357537
Session 7
Topic: Antifungal drug
Video on Antifungal drug
https://www.youtube.com/watch?v=qkmF-kA0AR8
https://www.youtube.com/watch?v=bBqouzQktx8
https://www.youtube.com/watch?v=YhQu-ZgNs8g
Slideshare link to Antifungal drug
https://www.slideshare.net/samghany/antifungal-drugs-89498931
https://www.slideshare.net/Vijayanarasimha1/antifungal-agents-64899496
Session 8
Topic: Antiviral drug
Video on Antiviral drug
https://www.youtube.com/watch?v=R0tp26mTVz4
https://www.youtube.com/watch?v=VQLRbChcd3c
https://www.youtube.com/watch?v=67Qjv88Z52w
Slideshare link to Antiviral drug
https://www.slideshare.net/doctorrao/antiviral-drugs-15231072
https://www.slideshare.net/KrushangiShah233/anti-viral-22060253
Session 9
Topic: Production of penicillin
Video on Production of penicillin
https://www.youtube.com/watch?v=896YNlWxZI0
https://www.youtube.com/watch?v=vw6zOT9wXHw
https://www.youtube.com/watch?v=RgQBwZxTt64
Slideshare link to Production of penicillin
https://www.slideshare.net/AmitSahoo16/penicillin-production-73393680
Session 10
Practice session
Topic: Sterilization methods used in Microbiology Lab
Video on Sterilization methods used in Microbiology Lab
https://www.youtube.com/watch?v=bRadiLXkqoU
https://www.youtube.com/watch?v=MreCMjgONo0
Procedure to perform Sterilization methods used in Microbiology Lab
https://800ezmicro.com/proper-sterilization-in-the-modern-laboratory.html
https://ehs.colorado.edu/resources/disinfectants-and-sterilization-methods/
Session 11
Practice session
Topic: Isolation of human pathogens in sterile condition
Video on Isolation of human pathogens in sterile condition
https://www.youtube.com/watch?v=949qQtAnjIc
http://labsforlife.in/InstructionalVideo.aspx
Procedure to perform Isolation of human pathogens in sterile condition
Session 12
Topic: Production of Streptomycin
Video on Production of Streptomycin
https://www.youtube.com/watch?v=PsksmAXwb10
Slideshare link to Production of Streptomycin
https://www.slideshare.net/SoodShipra/streptomycin-production
Session 13
Topic: Assessment of New Antibiotics
Video on Assessment of New Antibiotics
Session 14
Topic: Parameters for determination of the usefulness of antibiotics (in vitro and in vivo)
Video on Parameters for determination of the usefulness of antibiotics
Session 15
Topic: Pharmacokinetics of Antimicrobial agents
Video on Pharmacokinetics of Antimicrobial agents
https://www.youtube.com/watch?v=8-Qtd6RhfVA
https://www.youtube.com/watch?v=pWW-aq7iSa0
https://www.youtube.com/watch?v=6erefsWCVxg
https://www.youtube.com/watch?v=ztsBn8gsfHw
Slideshare link to Pharmacokinetics of Antimicrobial agents
https://www.slideshare.net/birhanugeta/pharmacology-of-antibacterial-agents
Session 16
Topic: Pharmacodynamics of Antimicrobial agents
Video on Pharmacodynamics of Antimicrobial agents
https://www.youtube.com/watch?v=01WrflemxK0
https://www.youtube.com/watch?v=tobx537kFaI
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3675903/
Slideshare link to Pharmacodynamics of Antimicrobial agents
https://www.slideshare.net/hwrain/pharmacodynamics-of-antibiotics
Session 17
Topic: Antibiotic Assay: Microbiological methods
Video on Antibiotic Assay: Microbiological methods
https://www.youtube.com/watch?v=sx1uDYSfINA
Slideshare link to Antibiotic Assay: Microbiological methods
https://www.slideshare.net/SonamkzBhutia/microbiological-assay-186636779
Session 18
Topic: Disc diffusion technique
Video on Disc diffusion technique
https://www.youtube.com/watch?v=BXr_kcki4Ag
https://www.youtube.com/watch?v=XhyMUHVbrlI
Slideshare link to Disc diffusion technique
Session 19
Session 20
Topic: Microbial Spoilage of Pharmaceutical Products
Video on Microbial Spoilage of Pharmaceutical Products
https://www.youtube.com/watch?v=DtHj396-3rI
https://www.youtube.com/watch?v=YIvXn-JInmc
https://www.youtube.com/watch?v=easKaFoVhLs
Slideshare link to Microbial Spoilage of Pharmaceutical Products
https://www.slideshare.net/SonamkzBhutia/pharmaceutical-spoilage
Session 21
Topic: Preservation of Pharmaceutical Products
Video on Preservation of Pharmaceutical Products
https://www.youtube.com/watch?v=I_1jNtkVWH4
Slideshare link to Preservation of Pharmaceutical Products
https://www.slideshare.net/SonamkzBhutia/preservation-of-pharmaceutical-products-191653694
https://www.slideshare.net/SoniaNazir/pharmaceutical-preservatives
Session 22
Practice session
Topic: Disc Test for Bacteriostatic and Bactericidal activity
Video on Disc Test for Bacteriostatic and Bactericidal activity
https://www.youtube.com/watch?v=sx1uDYSfINA&t=28s
https://www.youtube.com/watch?v=BXr_kcki4Ag&t=11s
Procedure to perform Disc Test for Bacteriostatic and Bactericidal activity
https://courses.lumenlearning.com/microbiology/chapter/testing-the-effectiveness-of-antimicrobials/
Session 23
Practice session
Topic: Dilution Test for Bacteriostatic and Bactericidal activity
Video on Dilution Test for Bacteriostatic and Bactericidal activity
https://www.youtube.com/watch?v=XhyMUHVbrlI
Procedure to perform Dilution Test for Bacteriostatic and Bactericidal activity
https://courses.lumenlearning.com/microbiology/chapter/testing-the-effectiveness-of-antimicrobials/
Session 24
Topic: Mode of action of Antibiotics on cell wall
Video on Mode of action of Antibiotics on cell wall
https://app.lecturio.com/#/lecture/s/6956/23490
https://www.youtube.com/watch?v=IVBCrzjOl40
https://www.youtube.com/watch?v=NGwP471sehI
Slideshare link to Mode of action of Antibiotics on cell wall
https://www.slideshare.net/YazanKherallah/antibiotic-mechanisms-of-action
Session 25
Topic: Mode of action of Antibiotic on cytoplasmic membrane
Video on Mode of action of Antibiotic on cytoplasmic membrane
https://www.youtube.com/watch?v=IVBCrzjOl40
https://www.youtube.com/watch?v=mMk6VWVpRpo&t=9s
Slideshare link to Mode of action of Antibiotic on cytoplasmic membrane
https://www.slideshare.net/YazanKherallah/antibiotic-mechanisms-of-action
Session 26
Topic: Mode of action of Antibiotic on cytoplasm
Video on Mode of action of Antibiotic on cytoplasm
https://www.youtube.com/watch?v=IVBCrzjOl40
Slideshare link to Mode of action of Antibiotic on cytoplasm
https://www.slideshare.net/blackbold14/antibiotics-mechanisms-of-actions
Session 27
Topic: Mode of action of Antibiotics on cell compounds
Video on Mode of action of Antibiotics on cell compounds
https://app.lecturio.com/#/lecture/s/6956/23504
Slideshare link to Mode of action of Antibiotics on cell compounds
Session 28
Topic: Bacterial Resistance to Antibiotics
Video on Bacterial Resistance to Antibiotics
https://www.youtube.com/watch?v=057phDG4mKU&t=12s
https://www.youtube.com/watch?v=057phDG4mKU&t=7s
https://www.youtube.com/watch?v=mMk6VWVpRpo&t=4s
Slideshare link to Bacterial Resistance to Antibiotics
https://www.slideshare.net/nasertadvi/antibiotic-resistance-14709382
Session 29
Topic: Biochemical mechanisms of resistance
Video on Biochemical mechanisms of resistance
https://www.youtube.com/watch?v=057phDG4mKU&t=12s
Slideshare link to Biochemical mechanisms of resistance
https://www.slideshare.net/amerali6/antimicrobial-resistance-amr-187806112
Session 30
Topic: Genetic basis of antibiotic resistance
Video on Genetic basis of antibiotic resistance
Session 31
Topic: Problems in antibiotic therapy due to resistance
Video on Problems in antibiotic therapy due to resistance
Session 32
Practice session
Topic: Screening of herbal plants for Antibiotic activity
Video on Screening of herbal plants for Antibiotic activity
https://www.youtube.com/watch?v=hejV-wmuZio
https://www.youtube.com/watch?v=HpIuwQHGoDI
Procedure to perform Screening of herbal plants for Antibiotic activity
Session 33
Practice session
Topic: Sterility testing of pharmaceuticals
Procedure to perform Sterility testing of pharmaceuticals
https://gibraltarlabsinc.com/services/microbiology/sterility-testing/
https://www.pharmaguideline.com/2011/11/sop-for-procedure-for-sterility-testing.html#gsc.tab=0
Session 34
Practice session
Topic: Bacteriological analysis of water
Video on Bacteriological analysis of water
https://www.youtube.com/watch?v=LpDjGCsmxZI
Procedure to perform Bacteriological analysis of water
https://www.researchgate.net/publication/330401314_Bacteriological_water_analysis
https://www.who.int/water_sanitation_health/resourcesquality/wqmchap10.pdf
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Dr. Soumya Jal is working at Centurion University of Technology and Management since 2018, prior to which she was associated with Rama Devi Women’s University as a visiting faculty. She has completed her Ph. D. in Medical Biotechnology from VIT University, Vellore (2016). Her research area includes fields such as, Applied and Clinical Microbiology, Molecular […]