Course Name: Phytochemistry and Toxicology
Code (Credit): CUTM2380 (3-1-0)
Objective:
To know the techniques of isolation, purification and identification of phyto-molecules.
To evaluate the toxicity of a substance.
Course outcome
COs |
Course outcomes |
Mapping COs with POs (High-3, Medium-2, Low-1) |
CO1 |
Able to gain Knowledge on Toxicology. It helps in assessing the fate of foreign chemicals in the environment and of their effects on different biological organization levels. |
PO1 (3) |
CO2 |
Research and problem Solving |
PO2(3), PO7(3) |
CO7 |
Environment and Sustainability |
PO7(3) |
CO8 |
Ethics and Responsibility |
PO8(3), PO7(3) |
After completion of this course a student will be able
To do the extraction of phyto-molecules.
To identify the phyto-molecules
To purify the phyto-molecules
To asses the toxicity level in a substance
Course Syllabus
Module I (8 h)
Basics of Phytochemistry, isolation, purification and identification techniques of phyto-molecules.
Practice 1: Isolation of curcumin from Turmeric
Practice 1: Isolation of caffeine from coffe
Module-II
Modern methods of extraction, application of latest techniques like Spectroscopy, chromatography and electrophoresis
Practice 3: Monitoring the isolation of curcumin from turmeric using TLC
Practice 4: Monitoring the isolation of caffeine from coffe using TLC
Module III
Isolation, Identification and Analysis of Phytoconstituents
- Terpenoids: Menthol, Citral, Artemisin
- Glycosides: Glycyrhetinic acid & Rutin
Practice 5: Purification crude curcumin using column chromatography
Module IV
Isolation, Identification and Analysis of Phytoconstituents
- Alkaloids: Atropine,Quinine,Reserpine,Caffeine
- Resins: Podophyllotoxin, Curcumin
Practice 5: Purification crude caffeine using column chromatography
Module V
Industrial production, estimation and utilization of the following phytoconstituents: Curcumin, Atropine, Podophyllotoxin, Caffeine, Taxol, Vincristine and Vinblastine
Module- VI
Introduction to toxicology, Background of Toxicology, Subdisciplines of toxicology, Acute, Sub acute and chronic toxicology,
Module-VII
Toxicity Pathways, Mechanism of toxicology, Adverse Outcome Pathways, Dose response relationship
Session Plan
Session 1
Basics of Phyto-chemistry
Session 2
Isolation Techniques
Session 3
Purification Techniques
Session 4
Identification Techniques
Session 5
Modern methods of extraction
Session 6
Application of latest techniques
Session 7
chromatography and electrophoresis
Session 8
Isolation of Terpinoids
Session 9
Isolation of Menthol, Citral, Artemisin
Session 10:
Isolation of Menthol, Citral, Artemisin
Isolation of Glycosides: Glycyrhetinic acid & Rutin
Session 11
Identification of Terpinoids
Session 12
Identification of Glycosides
Session 13
Analysis of Phyto-constituents
Session 14
Industrial production, estimation and utilization of Curcumin, Atropine, Podophyllotoxin
Session 15
Industrial production, estimation and utilization of Caffeine, Taxol, Vincristine and Vinblastine
Session 16
Introduction to toxicology
Session 17
Background of Toxicology
Session 18
Subdisciplines of toxicology
Session 19
Acute, Sub acute and chronic toxicology
Session 20
Toxicity Pathways, Mechanism of toxicology
Session 21
Adverse Outcome Pathways,
Session 22
Dose response relationship