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The objective of the course is to give students new knowledge by handling of classical and modern plant biotechnology processes
Understanding of biotechnological processes has also applicative value in pharmaceutical and food industry, in agriculture and in ecology.
Upon successful completion of this course, the student will be able to:
Develop fundamental knowledge in Plant Molecular Biotechnology and its application in laboratory and industry settings.
Get hands on training with some of the most basic, techniques in micropropagation.
Plant Tissue Culture Historical perspective; Composition of media; Nutrient and hormone requirements (role of vitamins and hormones); Totipotency; Organogenesis; Embryogenesis (somatic and zygotic); Protoplast isolation, culture and fusion;
Practice1: Preparation of MS medium.
Practice2: Demonstration of in vitro sterilization and inoculation methods using leaf explants of tobacco, Datura, Brassica etc.
Practice3: Demonstration of in vitro sterilization and inoculation methods using nodal explants of tobacco, Datura, Brassica etc.
Practice4: Isolation of protoplasts.
Tissue culture applications (micropropagation, and regenesis, virus elimination, secondary metabolite production, haploids, triploids and hybrids; Cryopreservation, Germplasm Conservation).
Practice5: Study of anther, embryo and endosperm culture micro propagation, micropropagation, somatic embryogenesis & artificial seeds through photographs
Recombinant DNA technology Restriction Endonucleases (History, Types I-IV, biological role and application) Restriction Mapping (Linear and Circular); Cloning Vectors: Prokaryotic (pUC 18 and pUC19, pBR322, Ti plasmid, BAC) Lambda phage, M13 phagemid, Cosmid, Shuttle vector; Eukaryotic Vectors (YAC).
Practice6: Restriction digestion and gel electrophoresis of plasmid DNA.
Practice7: Construction of restriction map of circular and linear DNA from the data provided
Gene Cloning Recombinant DNA, Bacterial Transformation and selection of recombinant clones, PCR-mediated gene cloning; Gene Construct; construction of genomic and cDNA libraries, screening DNA libraries to obtain gene of interest by genetic selection; complementation, colony hybridization; PCR
Practice 8: Isolation of plasmid DNA.
Methods of gene transfer Agrobacterium-mediated, Direct gene transfer By Electroporation, Microinjection, Microprojectile bombardment; Selection of transgenics– selectable marker and reporter genes (Luciferase, GUS, GFP).
Practice 9: Study of methods of Agrobacterium-mediated gene transfer through photographs
Practice 10: Study of methods of gene transfer through photographs: direct gene transfer by electroporation, microinjection, microprojectile bombardment.
Applications of Biotechnology Pest resistant (Bt-cotton); herbicide resistant plants (Round Up Ready soybean); Transgenic crops with improved quality traits (Flavr Savr tomato, Golden rice); Improved horticultural varieties (Moondust carnations)
Practice11:Study of steps of genetic engineering for production of Bt cotton, Golden rice through photographs.
Practice12: Study of steps of genetic engineering for production of Flavr Savr tomato through photographs
Role of transgenics in bioremediation (Superbug); edible vaccines; Industrial enzymes (Aspergillase, Protease, Lipase); Genetically Engineered Products–Human Growth Hormone; Humulin; Biosafety concerns.
Bhojwani, S.S. and Razdan, M.K., (1996). Plant Tissue Culture: Theory and Practice. Elsevier Science Amsterdam.The Netherlands.
Glick, B.R., Pasternak, J.J. (2003). MolecularBiotechnology- Principles and Applications of recombinant DNA. ASM Press, Washington.
Bhojwani, S.S. and Bhatnagar, S.P. (2011). The Embryology of Angiosperms.Vikas Publication House Pvt. Ltd., NewDelhi. 5thedition.
Snustad, D.P. and Simmons, M.J. (2010). Principles of Genetics. John Wiley and Sons, U.K. 5thedition.
Stewart, C.N. Jr. (2008). Plant Biotechnology & Genetics: Principles, Techniques and Applications.John Wiley & Sons Inc. U.S.A.
Study of anther, embryo and endosperm culture micro propagation, micropropagation, somatic embryogenesis & artificial seeds through photographs.
Dr. Shantanu Bhattacharyya works as an Assistant Professor, Department of Botany, at the Centurion University of Technology and Management, Bolangir Campus, Odisha, India. He has received his M.Sc. (Life Science- Botany) and M.Phil (Life Sciences) from Sambalpur University, Orissa, India and B.Ed. from Singhania University, Rajasthan India. He qualified GATE-2007, CSIR-UGC NET LS -2008. He […]
Dr Rukmini Mishra is presently working as an Assistant Professor (Botany), School of Applied Science, Centurion University of Technology and Management. She has a PhD in Agricultural Biotechnology from ICAR-National Rice Research Institute, India. After PhD, she received the prestigious Young Scientist Award from the Dept. of Science & Technology, SERB, Govt. of India to […]