Plant Biotechnology

Teacher

Dr. Shantanu Bhattacharyya

Category

Core Courses

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Course Name : Plant Biotechnology

Code(Credit) : CUTM1468(3-2-1)

Course Objectives

The objective of the course is to give students new knowledge by handling of classical and modern plant biotechnology processes

Understanding of biotechnological processes has also applicative value in pharmaceutical and food industry, in agriculture and in ecology.

Course Outcomes

COs Course outcomes Mapping COs with POs (High-3, Medium-2, Low-1)
CO1 Develop fundamental knowledge in Plant Molecular Biotechnology and its application in laboratory and industry settings PO1-3, PO2-3, PO3-2, PO4-3, PO7-2
CO2 Laboratory skills on integrative approach for biotechnological experiments specifically on basic, techniques in micro-propagation PO1-3, PO2-3, PO3-3, PO4-3, PO7-2
CO3 Design of experiments for complex scientific  problems towards conservation of plant species PO1-2, PO2-3, PO3-3, PO4-3, PO7-3
CO4 Experimental skills to conserve  plants for sustainability PO1-2, PO2-3, PO3-3, PO4-3, PO7-3

Course Syllabus

Module-I

Plant Tissue Culture Historical perspective; Composition of media; Nutrient and hormone requirements (role of vitamins and hormones); Totipotency; Organogenesis; Embryogenesis (somatic and zygotic); Protoplast isolation, culture and fusion;

 

Practice1: Preparation of MS medium.

Practice2: Demonstration of in vitro sterilization and inoculation methods using leaf explants of tobacco, Datura, Brassica etc.

Practice3: Demonstration of in vitro sterilization and inoculation methods using nodal explants of tobacco, Datura, Brassica etc.

Practice4: Isolation of protoplasts.

 

Module-II

 Tissue culture applications (micropropagation, and regenesis, virus elimination, secondary metabolite production, haploids, triploids and hybrids; Cryopreservation, Germplasm Conservation).

Practice5: Study of anther, embryo and endosperm culture micro propagation, micropropagation, somatic embryogenesis & artificial seeds through photographs

 

Module-III

 Recombinant DNA technology Restriction Endonucleases (History, Types I-IV, biological role and application) Restriction Mapping (Linear and Circular); Cloning Vectors: Prokaryotic (pUC 18 and pUC19, pBR322, Ti plasmid, BAC) Lambda phage, M13 phagemid, Cosmid, Shuttle vector; Eukaryotic Vectors (YAC).

 Practice6: Restriction digestion and gel electrophoresis of plasmid DNA.

Practice7: Construction of restriction map of circular and linear DNA from the data provided

 

Module-IV

 Gene Cloning Recombinant DNA, Bacterial Transformation and selection of recombinant clones, PCR-mediated gene cloning; Gene Construct; construction of genomic and cDNA libraries, screening DNA libraries to obtain gene of interest by genetic selection; complementation, colony hybridization; PCR

 

Practice 8: Isolation of plasmid DNA.

 

Module-V

 Methods of gene transfer Agrobacterium-mediated, Direct gene transfer By Electroporation, Microinjection, Microprojectile bombardment; Selection of transgenics– selectable marker and reporter genes (Luciferase, GUS, GFP).

 

Practice 9: Study of methods of Agrobacterium-mediated gene transfer through photographs

Practice 10: Study of methods of gene transfer through photographs: direct gene transfer by electroporation, microinjection, microprojectile bombardment.

 

 Module-VI

 Applications of Biotechnology Pest resistant (Bt-cotton); herbicide resistant plants (Round Up Ready soybean); Transgenic crops with improved quality traits (Flavr Savr tomato, Golden rice); Improved horticultural varieties (Moondust carnations)

Practice11:Study of steps of genetic engineering for production of Bt cotton, Golden rice through photographs.

Practice12: Study of steps of genetic engineering for production of Flavr Savr tomato through photographs

 

Module-VII

 Role of transgenics in bioremediation (Superbug); edible vaccines; Industrial enzymes (Aspergillase, Protease, Lipase); Genetically Engineered Products–Human Growth Hormone; Humulin; Biosafety concerns.

 

Text Book:

 Bhojwani, S.S. and Razdan, M.K., (1996). Plant Tissue Culture: Theory and Practice. Elsevier Science Amsterdam.The Netherlands.

Glick, B.R., Pasternak, J.J. (2003). MolecularBiotechnology- Principles and Applications of recombinant DNA. ASM Press, Washington.

Bhojwani, S.S. and Bhatnagar, S.P. (2011). The Embryology of Angiosperms.Vikas Publication House Pvt. Ltd., NewDelhi. 5thedition.

Snustad, D.P. and Simmons, M.J. (2010). Principles of Genetics. John Wiley and Sons, U.K. 5thedition.

 Reference Book:

Stewart, C.N. Jr. (2008). Plant Biotechnology & Genetics: Principles, Techniques and Applications.John Wiley & Sons Inc. U.S.A.

Session Plan

Session 1

Plant Tissue Culture, Historical perspective

https://youtu.be/xuwV3ywCxW8

Session 2

Session 3

Nutrient and hormone requirements (role of vitamins and hormones)

https://www.youtube.com/watch?v=LB6aJLJ5JmE

Session 5

Embryogenesis (somatic and zygotic)

https://www.youtube.com/watch?v=xjN1d7dfM94

Session 6

Protoplast isolation, culture and fusion

https://www.youtube.com/watch?v=utlKKvqxbWw

Session 7

Practice

Preparation of MS medium

https://www.youtube.com/watch?v=ltbdM3boWmU

Session 8

Practice

Demonstration of in vitro sterilization and inoculation methods using leaf explants of tobacco, Datura, Brassica etc.

https://www.youtube.com/watch?v=VrTBAQ3TbjQ.

Session 9

Practice

Demonstration of in vitro sterilization and inoculation methods using nodal explants of tobacco, Datura, Brassica etc.

https://www.youtube.com/watch?v=bRadiLXkqoU

Session 10

Practice

Isolation of protoplasts.

https://www.youtube.com/watch?v=8grwL8daO1Q.

Session 11

Tissue culture applications -micropropagation and regenesis

https://www.youtube.com/watch?v=IXM7UmvLKQ4

Session 12

Tissue culture applications:  virus elimination

https://www.youtube.com/watch?v=j2UFEK86x5U,

Session 13

Tissue culture applications:  secondary metabolite production.

https://www.youtube.com/watch?v=7rI-Lyftpd0.

Session 14

Tissue culture applications:  haploids, triploids and hybrids

https://www.youtube.com/watch?v=uEwH1M0bqb4

Session 15

Tissue culture applications:  Cryopreservation, Germplasm Conservation.

https://www.youtube.com/watch?v=5qXpvcaKFz4

https://www.youtube.com/watch?v=oEIHkChrRcg

Session 16

Practice

Study of anther, embryo and endosperm culture micro propagation, micropropagation, somatic embryogenesis & artificial seeds through photographs.

https://www.youtube.com/watch?v=IXM7UmvLKQ4

Session 18

Restriction Endonucleases (biological role and application)

https://www.youtube.com/watch?v=I7F4Xbjn73c

Session 19

Restriction Mapping (Linear and Circular)

https://www.youtube.com/watch?v=UUiRaOU1BNI

Session 20

Cloning Vectors: Prokaryotic (pUC 18 and pUC19)

https://www.youtube.com/watch?v=LP_7_7J4ZmU

Session 21

Cloning Vectors: Prokaryotic (pBR322, Ti plasmid, BAC)

pBR322

Ti plasmid

BAC

Session 22

Cloning Vectors: Lambda phage, M13 phagemid

Lambda phage

M13

Session 23

Cloning Vectors: Cosmid,  Shuttle vector

cosmid

shuttle vector

Session 24

Session 25

Practice

Restriction digestion and gel electrophoresis of plasmid DNA.

https://www.youtube.com/watch?v=3esTzddSgX8

Session 26

Practice

Construction of restriction map of circular and linear DNA from the data provided

circular dna

linear dna

Session 28

Bacterial Transformation and selection of recombinant clones

Bacterial Transformation

Session 29

Session 30

Gene Construct; construction of genomic libraries

Gene construct

construction of genomic libraries

Session 31

Construction of cDNA libraries

https://www.youtube.com/watch?v=URtcaoNEIrI

Session 32

Screening DNA libraries to obtain gene of interest by genetic selection

https://www.youtube.com/watch?v=3-Z5AkSdNpM

Session 33

Complementation, colony hybridization

complementation

colony hybridization

 

Session 34

Session 35

Practice

Study of anther, embryo and endosperm culture micro propagation, micropropagation, Isolation of plasmid DNA.

https://www.youtube.com/watch?v=R5EraRP3V-o

Session 36

Methods of gene transfer Agrobacterium-mediated gene transfer

https://www.youtube.com/watch?v=yesNHd9h8k0

Session 37

Direct gene transfer By Electroporation, Microinjection, Microprojectile bombardment

electroporation

microprojectile

Session 38

Selection of transgenics– selectable marker and reporter genes (Luciferase)

Reporter genes

Session 39

Selection of transgenics - reporter genes (GUS, GFP).

GFP

https://www.youtube.com/watch?v=x5ox71qIa-0

Session 40

Practice

Study of methods of Agrobacterium-mediated gene transfer through photographs.

https://www.youtube.com/watch?v=NXNFR4cj68U

Session 41

Practice

Study of methods of gene transfer through photographs:

Direct gene transfer by electroporation, microinjection, microprojectile bombardment.

https://www.youtube.com/watch?v=VqklR_8YRfA

https://www.youtube.com/watch?v=ulA8xsVji80

Session 43

Herbicide resistant plants (Round Up Ready soybean)

https://www.youtube.com/watch?v=l43P-Jauw6I&t=35s

Session 44

Transgenic crops with improved quality traits (Flavr Savr tomato, Golden rice)

flavr savr tomato

https://www.youtube.com/watch?v=0c-IjNqy-RU&t=15s

golden rice

Session 45

Improved horticultural varieties (Moondust carnations)

https://www.youtube.com/watch?v=WTLGkQoMJvo

Session 46

Practice

Study of steps of genetic engineering for production of Bt cotton, Golden rice through photographs.

https://www.youtube.com/watch?v=JtkhHIG3nx4

Session 47

Practice

Study of steps of genetic engineering for production of Flavr Savr tomato through photographs

https://www.youtube.com/watch?v=1jVAiPk1PCU

Session 48

Role of transgenics in bioremediation (Superbug)

https://www.youtube.com/watch?v=ByEjq7L5Ue4

Session 50

Session 51

Genetically Engineered Products–Human Growth Hormone

https://www.youtube.com/watch?v=4GmJ-KZOTZM

Session 52

Case Studies

Case Studies

Our Main Teachers

Dr. Shantanu Bhattacharyya

Assistant Professor, Department of Botany, School of Applied Science ( Bolangir Campus)
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Dr. Shantanu Bhattacharyya works as an Assistant Professor, Department of Botany, at the Centurion University of Technology and Management, Bolangir Campus, Odisha, India. He has received his M.Sc. (Life Science- Botany) and M.Phil (Life Sciences) from Sambalpur University, Orissa, India and B.Ed. from Singhania University, Rajasthan India. He qualified GATE-2007, CSIR-UGC NET LS -2008. He […]

Dr Rukmini Mishra

Assistant Professor
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Dr Rukmini Mishra is presently working as an Assistant Professor (Botany), School of Applied Science, Centurion University of Technology and Management. She has a PhD in Agricultural Biotechnology from ICAR-National Rice Research Institute, India. After PhD, she received the prestigious Young Scientist Award from the Dept. of Science & Technology, SERB, Govt. of India to […]