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•To understand the basics principles of plant sciences and molecular biology and their integration towards trait improvement in plants.
•To have a thorough knowledge of laboratory techniques used in plant biotechnology.
•To understand the industrial applications of biotechnology in developing new products.
•To undertake research in plant biotechnology.
•The students will understand the concepts and techniques of plant biotechnology and their applications in crop plants.
•They can get job opportunities in agribusiness, private companies, industries, universities and research laboratories.
•They can pursue higher studies and research in the field of plant biotechnology.
Module I: Basics of Tissue Culture
History, scope, concept of cellular differentiation and Totipotency, Cell culture media and sterilization techniques, Callus culture, nodal and tip culture, Protoplast and Embryo culture, Embryo culture and embryo rescue, protoplast isolation, culture and plant regeneration
Practice: 1. Preparation of tissue culture media
2. Direct Organogenesis: Shoot tip culture
Module II: Applications of Plant Tissue Culture
Somatic embryogenesis, Somaclonal variation and crop improvement, Germplasm conservation, Production of Secondary metabolites through Tissue culture, Industrial applications.
Practice: Micropogation techniques
Module III: Recombinant DNA technology
Genomic DNA & plasmid DNA isolation and purification, construction of
recombinant DNA and expression cassettes, Transformation (mobilization of vectors into competent bacteria), selection and analysis of recombinant clones, genomic DNA and cDNA libraries.
Practice: DNA isolation and purification
Module IV: Genetic Engineering in Plants
Vector mediated Gene transfer, Molecular basis of crown gall and hairy root diseases, features of Ti and Ri plasmids, mechanism of T-DNA transfer, role of virulence genes, vectors based on PTi & PRi, binary and co-integrate vectors, optimized protocols for Agrobacterium-mediated genetic transformation, physical and chemical methods of gene transfer.
Practice: 1. Callus induction
2. Agrobacterium mediated gene transfer in plants
Module V: Methods of Gene transfer in plants
Direct gene transfer methods (particle bombardment/ micro projectile / biolistic, electroporation, microinjection, liposome mediated, silicon carbide fibers), chemical methods (PEG - mediated, calcium phosphate co-precipitation), transgenic monocots and dicots via direct gene transfer, in plant transformation. Integration and fate of transgene, precision of transgene integration by site-specific.
Module VI: Applications of Genetic Engineering
Transgenic plants for disease resistance, nutritional improvement, herbicide tolerance,
Long shelf life, edible vaccines.
Module VII: Gene silencing in plants
Antisense RNA technology: Antisense RNA, construction of antisense vectors, applications of antisense technology. Gene silencing: causes (DNA methylation, homology-dependent suppression by antisense gene), strategies for avoiding gene silencing, methods of inducing gene silencing and its application. Regulatory RNA molecules (si RNA and miRNA), RNAi technology and its applications in plants.
Glick, B. R. and Pasternak (2003). Molecular Biotechnology: Principles and Applications of Recombinant DNA. ASM Press, Washington, D. C., USA.
Kyte, L. and Kleyn, J. (1996). Plants from Test Tube to: an Introduction to Micro propagation, 3rd Ed. Timber press, Port land, USA.
Pollard, W. J. and Walker (1990).Plant Cell and Tissue Culture Vol VI. Humana press Clifton, USA.
Dr Rukmini Mishra is presently working as an Assistant Professor (Botany), School of Applied Science, Centurion University of Technology and Management. She has a PhD in Agricultural Biotechnology from ICAR-National Rice Research Institute, India. After PhD, she received the prestigious Young Scientist Award from the Dept. of Science & Technology, SERB, Govt. of India to […]