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Plant Metabolism
Teacher
Dr. Shantanu Bhattacharyya
Category
Core Courses
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Course Name : Plant Metabolism
Code(Credit) : CUTM1467(3-2-1)
Course Objectives
Explain the purposes of and relationship between photosynthesis and respiration in plants.
Explain the significance of plant mass gain and loss to larger-scale ecosystem processes, such as the global carbon cycle.
Describe the sources and sinks involved in the acquisition and utilization of carbon in plant systems..
Learning Outcomes
Upon successful completion of this course, the student will be able to:
Understand on the kingdoms of biomolecules, metabolites and pathways that are the prerequisites and consequences of physiological phenomenon for further manipulations.
Develop integrative approach for visions in biological problems
Have a comprehensive understanding of the field of plant metabolism at a level that allows experimental implementation, including available methodologies and the historical development and current understanding of the field such that the interactions between various plant metabolic processes can be understood and appreciated
Course Syllabus
Module-I Concept of metabolism
Introduction,
Anabolic and Catabolic pathways,
Regulation of metabolism,
Role of regulatory enzymes (allosteric, covalent modulation and Isozymes).
Module-II Carbon assimilation
Historical background photosynthetic pigments,
Role of photosynthetic pigments (chlorophylls and accessory pigments), antenna molecules and reaction centres,
Photochemical reactions, photosynthetic electron transport, PSI, PSII, Qcycle,
CO2 reduction,
Photorespiration,
C4 pathways;
Crassulacean acid metabolism;
Factors affecting CO2 reduction.
Practice:
Chemical separation of photosynthetic pigments.
Experimental demonstration of Hill’s reaction.
To study the effect of light intensity on the rate of photosynthesis.
Effect of carbon dioxide on the rate of photosynthesis.
Demonstration of fluorescence by isolated chlorophyll pigments.
Demonstration of absorption spectrum of photosynthetic pigments
Module-III Carbohydrate metabolism
Synthesis and catabolism of sucrose and starch.
Carbon Oxidation
Glycolysis :
Fate of pyruvate,
Regulation of glycolysis,
Oxidative pentose phosphate pathway,
Oxidative Decarboxylation of Pyruvate,
Regulation of PDH, NADH shuttle;
TCA cycle,
Amphibolic role, Anaplerotic Reactions,
Regulation of the Cycle,
Mitochondrial Electron Transport,
Oxidative Phosphorylation,
Cyanide-Resistant Respiration,
Factors affecting respiration.
Practice
To compare the rate of respiration in different parts of a plant.
To compare the rate of respiration in different germinating Seed.
Module-IV ATP-Synthesis
Mechanism of ATP synthesis,
Substrate level phosphorylation,
Chemiosmotic mechanism(oxidative and photophosphorylation),
ATP synthase,
Boyers conformational model,
Role of uncouplers.
Module-V Lipid metabolism
Synthesis and breakdown of triglycerides,
β-oxidation,
glyoxylate cycle,
gluconeogenesis and its role in mobilisation of lipids during seed germination,
α oxidation.
Practice:
To study the activity of lipases in germinating oilseeds
Demonstration of mobilization of lipids during germination.
Module-VI Nitrogen metabolism
Nitrate assimilation,
Biological nitrogen fixation (examples of legumes and non-legumes);
Physiology and biochemistry of nitrogen fixation;
Ammonia assimilation and transamination.
Practice:
To demonstrate activity of Nitrate reductase in germinating leaves of Gram, Pea, Mung
To demonstrate activity of Nitrate reductase in germinating leaves of Rice, Wheat
Module-VII Mechanisms of signal transduction
Receptor-ligand interactions;
Second messenger concept,
Calcium calmodulin,
MAP kinase cascade
Text Book:
1. Hopkins, W.G. and Huner, A. (2008). Introduction to Plant Physiology.John Wiley and Sons. U.S.A. 4thedition.
2.Taiz, L., Zeiger, E., MØler, I.M. and Murphy, A (2015). Plant Physiology and Development.Sinauer Associates Inc. USA. 6thedition.
Reference Book:
Harborne, J.B. (1973). Phytochemical Methods.John Wiley & Sons. New York
Session Plan
Session 1
Session 2 (Assignment 1)
Introduction, anabolic and catabolic pathways,
https://cnx.org/contents/GFy_h8cu@9.85:7V33N3f_@8/Energy-and-Metabolism
Session 3
Session 4
Role of regulatory enzymes (allosteric, covalent modulation and Isozymes).
Session 6 ( Assignment 2)
Historical background photosynthetic pigments,Role of photosynthetic pigments(chlorophylls and accessory pigments), antenna molecules and reaction centres,
http://www.life.illinois.edu/govindjee/photosynBook/Chapter9.pdf
Session 7 (Practice 1)
Chemical separation of photosynthetic pigments.
Session 8 (Practice 2)
Demonstration of absorption spectrum of photosynthetic pigments
Session 9
Photochemical reactions, photosynthetic electron transport,PSI, PSII,Qcycle,
https://www.youtube.com/watch?v=OC677e1DpUc
Session 10 (Practice 3)
Experimental demonstration of Hill’s reaction.
http://people.hsc.edu/faculty-staff/edevlin/edsweb01/courses/Cellbiology/labmanual/new_page_4.htm
Session 11 (Practice 4)
To study the effect of light intensity on the rate of photosynthesis.
http://brilliantbiologystudent.weebly.com/effect-of-light-intensity.html#:~:text=Observation%2FResults,photosynthesis%20increases%20with%20light%20intensity. https://www.youtube.com/watch?v=XvScJqzRR24
Session 12 ( Assignment 3)
CO2 Reduction, Factors affecting CO2 reduction.
Factors Affecting Co2 reduction
https://www.youtube.com/watch?v=PQ1zZ9G5d7M
Session 13 (Practice 5)
Effect of carbon dioxide on the rate of photosynthesis.
Session 14
Session 16 (Practice 6)
Demonstration of fluorescence by isolated chlorophyll pigments
Session 17 (Assignment 4)
Session 18
Synthesis and catabolism of sucrose and starch.
http://coursecontent.gvltec.edu/gollwcegweb/bio211/211%20Lecture/Lec%2025%20FA12/Lec%2025%20FA12.ppt
Session 20
Glycolysis :Fate of pyruvate,Regulation of glycolysis,
Session 22
Oxidative decarboxylation of pyruvate,Regulation of PDH,NADH shuttle
Session 23
TCA cycle, amphibolic role, anaplerotic reactions,Regulation of the cycle
Session 24
Mitochondrial electron transport,Oxidative phosphorylation,, Cyanide-resistant respiration,
Session 25 (Assignment 6)
Factors affecting respiration.
Session 26 (Practice 7)
To compare the rate of respiration in different parts of a plant.
Session 27
ATP Synthesis, Mechanism of ATP synthesis,
Session 28 (Assignment 7)
Substrate Level Phosphorylation,
Session 29 (Assignment 8)
Session 30
Session 31
ATP synthase, Boyers conformational model, role of uncouplers.
https://www.nobelprize.org/prizes/chemistry/1997/boyer/25946-the-binding-change-mechanism/
Session 32 (Practice 8)
To compare the rate of respiration in different germinating Seed.
Session 33 (Assignment 9)
Session 36 (Practice 9)
To study the activity of lipases in germinating oilseeds and demonstrate mobilization of lipids during germination
Session 39
Role of gluconeogenesis in mobilisation of lipids during seed germination,
Session 40 (Practice 10)
Demonstration of mobilization of lipids during germination
Session 42 (Practice 11)
To demonstrate activity of Nitrate reductase in germinating leaves of Gram, Pea, Mung
Session 44
biological nitrogen fixation (examples of legumes and non-legumes);
Biological Nitrogen FixationPDF
Session 46 (Practice 12)
To demonstrate activity of Nitrate reductase in germinating leaves of Rice, Wheat
Session 47
Ammonia assimilation and transamination.
ammonia assimilation and transamination PDF
Case Studies
Our Main Teachers
Dr. Shantanu Bhattacharyya
Assistant Professor, Department of Botany, School of Applied Science ( Bolangir Campus)
VIEW PROFILEDr. Shantanu Bhattacharyya works as an Assistant Professor, Department of Botany, at the Centurion University of Technology and Management, Bolangir Campus, Odisha, India. He has received his M.Sc. (Life Science- Botany) and M.Phil (Life Sciences) from Sambalpur University, Orissa, India and B.Ed. from Singhania University, Rajasthan India. He qualified GATE-2007, CSIR-UGC NET LS -2008. He […]