Plant Tissue Culture Engineering
Teacher
Debanjana Saha
Category
Core Courses
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Course Name : Plant Tissue Culture Engineering
Code(Credit) : CUTM2363(3-1-0)
Course Objectives
- To understand a procedure that is often used to propagate many plants of the same genetic background.
- To make the students understand the concept of this techniques and its application
Learning Outcomes
- To grasp the information of transgenic plants in addition to industrial and agricultural applications of plant biotechnology.
Course Syllabus
Module I: Plant genomes and plant tissue culture (4hrs)
Introduction-gene structure and genome size and organization, Brief history of plant tissue culture, Scope of plant tissue culture,Laboratory instructions and maintenance of sterilization, composition of media, nutrient and hormone requirement, mode of action of auxin and cytokinin. Plant tissue culture plasticity and totipotency
Practice: Laboratory set up and instrumentation
Module II: Types of tissue culture(4hrs)
Criteria of cell selection, factor affecting the culture of cells. Cell, Callus & Suspension cultures. Somatic Embryogenesis and Hybridization.Haploid Production through Anther and ovule culture; Embryo culture.
Practice: Sterilization Technique
Module III: In-vitro mutagenesis and germplasm Conservation(4hrs)
Somaclonal variation; In vitro mutation methods; Micropropagation somatic hybrid and cybrid,Plant regeneration. Germ plasm conservation, production of secondary metabolites; Sources of plant secondary metabolite
Practice: Media preparations: MS media, and rooting media
Module IV: Biotransformation(5hrs)
Gene constructs, Vectors for the production of transgenic plants, Introduction- Agrobacterium mediated gene transfer –Ti-plasmid-process of T-DNA transfer and integration, transformation in plant, Direct and indirect gene transfer methods. Binary vectors- basic features of vectors-optimization-clean gene technology.
Practice: Explant preparation, inoculation, and initiation of tissue culture
Module V: Transgenic Plants (5hrs)
Herbicide and Pest resistance, Herbicide resistance-use of herbicide in modern agriculture strategies for engineering herbicide resistance environment impact, pest resistance-nature and scale of insect/pest damage to crop. GM strategies-Bt approach to insect resistance-copy nature strategy-insect resistant crops and food safety.
Practice:Callus formation, multiplication and organogenesis
Module VI: Molecular farming (4hrs)
Introduction –carbohydrates and lipid production, molecular farming of proteins, economic considerations for molecular farming.GM crops- current status concerns about GM crops-regulations on GM crops and products, Greener genetic engineering.
Practice:.suspension culture
Module VII: Plant molecular biology(4Hrs)
Genome organization in prokaryotes and eukaryotes. Transcription and post-transcriptional regulation. Translation and post-translational process. Molecular basis of mutations.
Practice: Meristem Culture, Culture types:Anther Culture,Ovary culture
Text Books:
- Bhojwani,Sant Saran,Dantu,Prem Kumar, Plant tissue culture: An introductory text,Springer publication,3rd Edition
- Slater.A., Nigel W.S,Flower. R.Mark , Plant Biotechnology: The Genetic Manipulation of Plants, 2009, Oxford Univesity Press.
Reference Books:
- S Chawla,Introduction to Plant Biotechnology oxford and ibh publishing,third edition
- Robert H.Smith,Plant tissue culture techniques and experiments,third edition,Aademic Press
Session 2
Brief history of plant tissue culture, Scope of plant tissue culture, Laboratory instructions and maintenance of sterilization
Session 3
composition of media, nutrient and hormone requirement
Session 4( Project)
EPIGENETICS IN PLANT TISSUE CULTURE
Session 6
Session 9
Session 11
Session 12(Project)
Biotransformations With Plant Tissue Cultures
Session 15
Session 18(Project)
Tissue culture as a plant production technique for horticultural crops
Session 19
production of secondary metabolites; Sources of plant secondary metabolite
Session 20(Practice)
Explant preparation, inoculation and initiation of tissue culture
Session 22
Introduction- Agrobacterium mediated gene transfer –Ti-plasmid-process of T-DNA transfer and integration, transformation in plant,
T-DNA transfer
Session 23(Project)
Effects of ionizing radiation on plant tissue culture
Session 23
Session 25
Binary vectors- basic features of vectors-optimization-clean gene technology.
Session 26
Herbicide and Pest resistance,Herbicide resistance-use of herbicide in modern agriculture
Session 27(Project)
WATER RETENTION CAPACITY OF TISSUE CULTURED PLANTS
Session 28
strategies for engineering herbicideResistance environment impact
Session 29
pest resistance-nature and scale of insect / pest damage to crop
Session 30
GM strategies-Bt approach to insect resistance-copy nature strategy-insect resistant crops and food safety.
Session 35(Project)
Plant regeneration through tissue culture
Session 39(Project)
Plant cell culture: A potential source of chemicals
Session 42
Role of activated charcoal in plant tissue culture
Session 45(Project)
Application of Plant Cell and Tissue Culture for the Production of Phytochemicals in Medicinal Plants
Case Studies
Case Studies
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