Plant Tissue Culture Engineering

Teacher

Debanjana Saha

Category

Core Courses

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Course Name : Plant Tissue Culture Engineering

Code(Credit) : CUTM2363(3-1-0)

Course Objectives

  • To understand a procedure that is often used to propagate many plants of the same genetic background.
  • To make the students understand the concept of this techniques and its application

Learning Outcomes

  • To grasp the information of transgenic plants in addition to industrial and agricultural applications of plant biotechnology.

Course Syllabus

 Module I: Plant genomes and plant tissue culture (4hrs)

Introduction-gene structure and genome size and organization, Brief history of plant tissue culture, Scope of plant tissue culture,Laboratory instructions and maintenance of sterilization, composition of media, nutrient and hormone requirement, mode of action of auxin and cytokinin. Plant tissue culture plasticity and totipotency 

Practice: Laboratory set up and instrumentation

 Module II: Types of tissue culture(4hrs)

Criteria of cell selection, factor affecting the culture of cells. Cell, Callus & Suspension cultures. Somatic Embryogenesis and Hybridization.Haploid Production through Anther and ovule culture; Embryo culture.

Practice: Sterilization Technique

Module III: In-vitro mutagenesis and germplasm Conservation(4hrs)

Somaclonal variation; In vitro mutation methods; Micropropagation somatic hybrid and cybrid,Plant regeneration. Germ plasm conservation, production of secondary metabolites; Sources of plant secondary metabolite

Practice: Media preparations: MS media, and rooting media

  Module IV: Biotransformation(5hrs)

Gene constructs, Vectors for the production of transgenic plants, Introduction- Agrobacterium mediated gene transfer –Ti-plasmid-process of T-DNA transfer and integration, transformation in plant, Direct and indirect gene transfer methods. Binary vectors- basic features of vectors-optimization-clean gene technology.

Practice: Explant preparation, inoculation, and initiation of tissue culture

Module V: Transgenic Plants (5hrs)

Herbicide and Pest resistance, Herbicide resistance-use of herbicide in modern agriculture strategies for engineering herbicide resistance environment impact, pest resistance-nature and scale of insect/pest damage to crop. GM strategies-Bt approach to insect resistance-copy nature strategy-insect resistant crops and food safety.

Practice:Callus formation, multiplication and organogenesis

Module VI:   Molecular farming  (4hrs)

Introduction –carbohydrates and lipid production, molecular farming of proteins, economic considerations for molecular farming.GM crops- current status concerns about GM crops-regulations on GM crops and products, Greener genetic engineering.

Practice:.suspension culture

Module VII: Plant molecular biology(4Hrs)  

Genome organization in prokaryotes and eukaryotes. Transcription and post-transcriptional regulation. Translation and post-translational process. Molecular basis of mutations.

Practice: Meristem Culture, Culture types:Anther Culture,Ovary culture

 Text Books:

  1. Bhojwani,Sant Saran,Dantu,Prem Kumar, Plant tissue culture: An introductory text,Springer publication,3rd Edition
  1. Slater.A., Nigel W.S,Flower. R.Mark , Plant Biotechnology: The Genetic Manipulation of Plants, 2009, Oxford Univesity Press.

 

Reference Books:

  1. S Chawla,Introduction to Plant Biotechnology oxford and ibh publishing,third edition
  2. Robert H.Smith,Plant tissue culture techniques and experiments,third edition,Aademic Press

Session Plan

Session 1

Introduction-gene structure and genome size and organization

gene structure

Session 2

Brief history of plant tissue culture, Scope of plant tissue culture, Laboratory instructions and maintenance of sterilization

Plant Tissue culture introduction

introduction to Plant tissue culture

Session 3

composition of media, nutrient and hormone requirement

Media preparation

introduction to Plant tissue culture

Session 4( Project)

EPIGENETICS IN PLANT TISSUE CULTURE

Session 5

mode of action of auxin and cytokinin.,

plant hormone in tissue culture 

Session 6

Plant tissue culture plasticity and totipotency

totipotency

introduction to Plant tissue culture

Session 7( Practice)

Laboratory setup and instrumentation

Plant tissue culture laboratory 

Session 8

Criteria of cell selection,factor affecting the culture of cells

cell selection

Session 9

Cell, Callus & Suspension cultures

callus culture

suspension culture

Types of tissue culture

Session 10

Somatic Embryogenesis and Hybridization

somatic embryogenesis

Types of tissue culture

Session 11

Haploid Production through Anther and ovule culture

anther culture

Types of tissue culture

Session 12(Project)

Biotransformations With Plant Tissue Cultures

Session 13

Embryo culture
embryo culture

Session 14(Practice)

Sterilization Technique

sterilization 

Session 15

Somaclonal variation; In vitro mutation methods

somaclonal variation

somaclonal variation

Session 16

Micropropagation somatic hybrid and cybrid,Plant regeneration.

somatic hybridizationn

Session 17

Session 18(Project)

Tissue culture as a plant production technique for horticultural crops

Session 19

production of secondary metabolites; Sources of plant secondary metabolite

secondary metabolite

secondarymetabolites

Session 20(Practice)

Explant preparation, inoculation and initiation of tissue culture

Tissue culture process

Session 21

Gene constructs, Vectors for the production of transgenic plants,
transgenic

genetransformationmethods

Session 22

Introduction- Agrobacterium mediated gene transfer –Ti-plasmid-process of T-DNA transfer and integration, transformation in plant,
T-DNA transfer

genetransformationmethods

Session 23(Project)

Effects of  ionizing radiation on plant tissue culture

Session 23

Direct and indirect gene transfer methods.

gene transfer methods

genetransformationmethods

Session 24( Practice)

Media preparations: MS media,and rooting media

Media preparation 

Session 25

Binary vectors- basic features of vectors-optimization-clean gene technology.

Binary vector

  cleangenetechnology

Session 26

Herbicide and Pest resistance,Herbicide resistance-use of herbicide in modern agriculture

herbicide resistance

https://www.youtube.com/watch?v=A5p8EoIX7AQ

pest and herbicide resistance

Session 27(Project)

WATER RETENTION CAPACITY OF TISSUE CULTURED PLANTS

Session 28

strategies for engineering herbicideResistance environment impact

stratagies

genetransformationmethodspest and herbicide resistance

Session 29

pest resistance-nature and scale of insect / pest damage to crop

insect resistance strategies

pest and herbicide resistance

Session 30

GM strategies-Bt approach to insect resistance-copy nature strategy-insect resistant crops and food safety.

Bt crops                                                                                                               

Session 31(practice)

Callus formation, multiplication and organogenesis

organogenesis

Session 32

Session 33

Molecular farming of proteins

biopharming

molecularfarming

Session 35(Project)

Plant regeneration through tissue culture

Session 34

Session 36

regulations on GM crops and products,

GMOs regulation

Session 37

suspension culture, Meristem Culture

cell suspension culture
meristem culture

Session 39(Project)

Plant cell culture: A potential source of chemicals

Session 38

Genome organization in prokaryotes and eukaryotes

genome organization 

Session 40

Transcription and post-transcriptional regulation.

Trnascriptional regualtion

Session 42

Role of activated charcoal in plant tissue culture

Session 41

Translation and post-translational process

post translational modifications 

Session 43

Molecular basis of mutations.

mutation

molecular basis of mutation

Session 44

Culture types:Anther Culture, Ovary culture

anther culture

Session 45(Project)

Application of Plant Cell and Tissue Culture for the Production of Phytochemicals in Medicinal Plants

Case Studies

Case Studies

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