Principles of Biotechnology

Course Objectives-

1. To understand the basics of Molecular biology, plant and microbial Biotechnology.
2. Importance and applications in agriculture, case studies and success stories.
3. Public education, perception, IPR and related issues

CO-PSO mapping table-

PSO1 : Impart knowledge and understanding of fundamental concepts and techniques of maintaining and enhancing soil fertility, crop production, crop management, crop improvement, biodiversity and sustainability of agriculture worldwide.

PSO2 : Develop the ability to know farming practices and their scope to improve in the rural areas and the environments.

PSO3 : Apply knowledge of basic science through Agricultural microbiology, Plant biochemistry and Biotechnology.

PSO4 : Apply knowledge of Agri-business management and finance to develop profitable agricultural system.

Course Syllabus-

Unit I

History, scope and importance of Biotechnology; Specializations in Agricultural Biotechnology: Genomics, Genetic engineering, Tissue Culture, Bio-fuel, Microbial Biotechnology, Food Biotechnology etc. Basics of Biotechnology, Primary metabolic pathways, Enzymes and its activities.

Unit II

Structure of DNA, RNA and protein, their physical and chemical properties. DNA function: Expression, exchange of genetic material, mutation. DNA modifying enzymes and vectors; Methods of recombinant DNA technology; Nucleic acid hybridization; DNA/RNA libraries; Applications of gene cloning in basic and applied research, Plant transformation: Gene transfer methods and applications of GM crops.

Unit III

Molecular analysis of nucleic acids -PCR and its application in agriculture and industry, Introduction to Molecular markers: RFLP, RAPD, SSR, SNP etc, and their applications; DNA sequencing, different methods; Plant cell and tissue culture techniques and their applications.Introduction to genomics, transcriptomics, ionomics, metabolomics and proteomics. Plant cell and tissue culture techniques and their applications.

Unit IV

Introduction to Emerging topics: Genome editing, gene silencing, Plant microbial interactions,Success stories in Biotechnology, Careers and employment in biotechnology. Public perception of biotechnology; Bio-safety and bioethics issues; Intellectual property rights in biotechnology.

Suggested Readings-

1.Watson, J. D., Baker, T. A., Bell, S. P., Gann, A., Levine, M & Losick R (2014) Molecular Biology of the Gene, 7th edition, Cold Spring Harbor Laboratory Press, New York.

2. Brown, T. A. (2010) Gene Cloning and DNA analysis an Introduction 6th edition, Wiley Blackwell.

3. Primrose, S. B. and Twyman, R. (2006) Principles of gene Manipulation 7th edition, Wiley Blackwell.

4. Singh, B. D, Biotechnology: Expanding Horizons (2012) 4th edition, Kalyani publisher, New Delhi, India

Theory Session Plan-

Question Bank-

http://courseware.cutm.ac.in/wp-content/uploads/2023/07/Principles-of-Biotechnology-Question-Bank.docx

Assignments-

http://courseware.cutm.ac.in/wp-content/uploads/2023/07/Principles-of-Biotechnology-Assignments.docx