PRINCIPLES OF GENETICS
Teacher
Dr. Niranjan Kumar Chaurasia
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COURSE CODE: MGPB 0501 (2-1-0)
COURSE OBJECTIVES:
Understanding the basic concepts of inheritance of genetic traits, helping students to develop their analytical, quantitative, and problem-solving skills from classical to molecular genetics.
COURSE OUTCOMES (COs)/PROGRAMME SPECIFIC OUTCOMES (PSOs) MAPPING:
| COs | PSOs |
| CO-1: Knowledge of principles of inheritance and advances in molecular genetics | PSO-1 |
| CO-2: Solve the problems on Mendelian inheritance and chromosome mapping, extraction of DNA, PCR amplification, electrophoresis, etc. | PSO-3 |
PROGRAMME SPECIFIC OUTCOMES (PSOs):
PSO1: Impart knowledge and understanding of fundamental concepts and techniques of maintaining and enhancing soil fertility, crop production, crop management, crop improvement, biodiversity, and sustainability of agriculture worldwide.
PSO3: Apply knowledge of basic science through Agricultural microbiology, Plant Biochemistry, and Biotechnology.
SYLLABUS (THEORY):
Beginning of genetics, early concepts of inheritance, Mendel’s laws; Discussion on Mendel’s paper, Chromosomal theory of inheritance; Multiple alleles, Gene interactions, Sex determination, differentiation and sex-linkage, Sex-influenced and sex-limited traits; Linkage-detection, estimation; Recombination and genetic mapping in eukaryotes, Somatic cell genetics, Extra chromosomal inheritance; Mendelian population, Random mating population, Frequencies of genes and genotypes, Causes of change and Hardy-Weinberg equilibrium; Nature, structure and replication of the genetic material; Organization of DNA in chromosomes, Genetic code; Protein biosynthesis; Genetic fine structure analysis, Allelic complementation, Split genes, overlapping genes, Pseudogenes, Ontogenesis, Gene families and clusters; Regulation of gene activity in prokaryotes and eukaryotes; Molecular mechanisms of mutation, repair, and suppression; Bacterial plasmids, insertion (IS) and transposable (Tn) elements; Molecular chaperones and gene expression; RNA editing; Gene isolation, synthesis and cloning, genomic and cDNA libraries; PCR based cloning, positional cloning; Nucleic acid hybridization and immunochemical detection; DNA sequencing; DNA restriction and modification, Anti-sense RNA and ribozymes; Micro-RNAs (miRNAs); Genomics and proteomics; metagenomics; Transgenic bacteria and bioethics; Gene silencing; genetics of mitochondria and chloroplasts; Concepts of Eugenics, Epigenetics, Genetic disorders.
SYLLABUS (PRACTICAL):
Laboratory exercises in probability and chi-square; Demonstration of genetic principles using laboratory organisms; Chromosome mapping using a three-point test cross; Tetrad analysis; Induction and detection of mutations through genetic tests; DNA extraction and PCR amplification; Electrophoresis: basic principles and running of amplified DNA; Extraction of proteins and isozymes; Use of Agrobacterium-mediated method and Biolistic gun; Detection of transgenes in the exposed plant material; Visit to transgenic glasshouse and learning the practical considerations.
REFERENCES:
Benjamin A. Pierce (2012) Genetics: A Conceptual Approach (4th edition)
Singh B D. (2009). Genetics. Kalyani Publishers (2nd Revised Edition)
Gardner, E J & Snustad, D P. (1991). Principles of Genetics. John Wiley & Sons. 8th ed. 2006
Klug, W S and Cummings, M R. (2003). Concepts of Genetics, Peterson Edu.
Strickberger, M W. (2005). Genetics (III Ed). Prentice Hall, New Delhi, India
SESSION PLAN (THEORY):
SESSION-I: Beginning of genetics
SESSION-2: Early concepts of inheritance, Mendel’s laws, Discussion on Mendel’s paper
SESSION-3: Chromosomal theory of inheritance
SESSION-5: Sex determination, differentiation and sex-linkage, Sex-influenced and sex-limited traits
SESSION-4: Gene interactions, Multiple alleles
SESSION-6: Linkage-detection, estimation
SESSION-7: Recombination and genetic mapping in eukaryotes, Chiasmata and the time of crossing over
SESSION-8: Somatic cell genetics, Extra chromosomal inheritance
SESSION-9: Mendelian population, Random mating population, Frequency of genes and genotypes, Causes of change: Hardy-Weinberg equilibrium.
SESSION-10: Nature, structure and replication of the genetic material
SESSION-11: Organization of DNA in chromosomes, Genetic code
SESSION-12: Protein biosynthesis
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SESSION-13: Genetic fine structure analysis, Allelic complementation,
SESSION-14: Split genes, overlapping genes, Pseudogenes, Ontogenesis, Gene families and clusters
SESSION-15: Regulation of gene activity in prokaryotes
SESSION-16: Regulation of gene activity in eukaryotes
SESSION-17: Molecular mechanism of mutation, repair and suppression -reverse mutations and suppressor mutations
SESSION-18: Bacterial plasmids, insertion (IS) and transposable (Tn) elements
SESSION-19: Molecular chaperones and gene expression
SESSION-20: RNA editing
SESSION-21: Gene isolation, synthesis and cloning
SESSION-22: Genomic and cDNA libraries, PCR based cloning, positional cloning
SESSION-23: Nucleic acid hybridization and immunochemical detection
SESSION-24: DNA sequencing
SESSION-25: DNA restriction and modification, ribozymes
SESSION-26: Gene silencing, Anti-sense RNA,
SESSION-27: Micro RNAs (miRNAs)
SESSION-28: Genomics and proteomics
SESSION-29: Metagenomics
SESSION-30: Transgenic bacteria and bioethics
SESSION-31: Genetics of mitochondria and chloroplasts
SESSION-32: Concepts of Eugenics, Epigenetics, Genetic disorders.
SESSION PLAN (PRACTICAL):
SESSION-1: Laboratory exercises in probability and chi-square
SESSION-2: Demonstration of genetic principles using laboratory
SESSION-3: Demonstration of genetic principles: Problems on monohybrid cross
SESSION-4: Demonstration of genetic principles: Problems on dihybrid cross
SESSION-5: Two-point test cross
SESSION-6: Chromosome mapping using three-point test cross, Tetrad analysis
SESSION-7: Extraction of total DNA by CTAB Method
SESSION-8: Quantification of DNA
SESSION-9: Electrophoresis - basic principles and running of amplified DNA
SESSION-10: PCR amplification
SESSION-11: Extraction of proteins (SDS-PAGE) and isozymes
SESSION-12: Use of Agrobacterium-mediated method
SESSION-13: Induction and detection of mutations through genetic tests
SESSION-14: Biolistic gun, practical demonstrations
SESSION-15: Detection of transgenes in the exposed plant material
SESSION-16: Visit to transgenic glasshouse and learning the practical considerations
E-STUDY MATERIALS:
Pierce Genetics Conceptual Approach 4th textbook
QUESTION BANK:
ASSIGNMENTS
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