Course Name: Super Critical Carbon Dioxide Plant Operation

Code (Credits): ( 0-2-2)

Course Description

1. Short description: Supercritical fluid extraction (SFE) is the process of separating one component (the extractant) from another (the matrix) using supercritical fluids as the extracting solvent. It is one of the most popular green extraction widely accepted in the industries. This certificate course aims to provide the trainees a hands-on practical experience of this extraction method along with the most updated knowledge on the SCFE technique.
2. Prerequisite: Basic knowledge of organic chemistry.
3. Duration of the course: One-month online theory class and 50 hours practical in PKD Campus or collaborative work with our Industrial Partners.
4. Key highlights

• Faculty: Dr. Preetha Bhadra, Dr. Rosy Mallik, Dr. Pradipta Banerjee
• The certificate will be issued jointly by Gram Tarang Foods Pvt. Ltd. & Centurion University of Technology and Management.
• Job Opportunity: In Pharmaceutical, Nutraceutical, Cosmetics Industry.
• Contact with Industry Personnel: Mr. Babu Shankar, MD, Gram Tarang Foods Pvt. Ltd.; md@gramtarangfoods.in

Course Syllabus

Module - I

Introduction to supercritical fluids (SCFs): Supercritical fluids and their properties, Examples of SCFs, Chemistry of SCFs

Module - II

Instrumentation: What are the different parts of SCF extraction plant, identification and learning the use of different parts of the SCF extraction unit, general maintenance and cleaning of the unit

Practice 1:

Visit to SCF extraction Unit of PKD Campus and hands on training on the instrumentation from the experts/engineers working there.

Module – III

Industrial application: Uses of SCF extraction in different industries (pharma, cosmetics, nutraceutical), SCF extraction is favoured over other conventional techniques, Why this technique is known as Green extraction technique.

Practice 2:

• Exposure visit to Gram Tarang Foods Pvt. Ltd. and other industrial partners.
• Real time interaction with extraction experts

Module – IV

Methodology: Raw materials for SCF extraction process, sample preparation, calculation of percentage yield of desired product

Practice 3:

• Collection of raw materials
• Sample preparation
• Extraction of ginger oil
• Extraction of turmeric oil
• Extraction of marigold
• Extraction from tulsi leaf
• Calculation of percentage yield

• Hands on training to troubleshoot different problems related to SCF extraction

Module – V

Modifiers: What are modifiers, role of modifiers in SCF extraction, organic modifier, food grade modifier

Practice 4:

Assignment (Any one)

• Modifier Effects on Supercritical CO₂ Extraction Efficiency
• Modifier Effects in the Supercritical Fluid Extraction of Solutes from Plant Materials

Module – VI

Purification of extract: A brief idea of downstream process of the SCF extracts, methods of purification of organic molecules, identification of organic molecules.

Practice 5:

• Freeze Drying
• Column chromatography
• Paper chromatography
• HPLC (optional)

Module – VII

Importance of SCF extraction: advantages of SCF extraction over other conventional techniques, limitations of SCF extraction, troubleshoot

Practice 6:

• Hands on training by GTF Pvt. Ltd. Experts on troubleshooting different probable problems that may occur while operating the SCF extraction unit
• Doubt clearing about the operation of SCF extraction process

Text Book:

Supercritical Carbon Dioxide Extraction Handbook (Publisher: Scholars’ Press) By P. Banerjee, R. Mallik and S. Maji

Reference Books:

1. Natural Extracts Using Supercritical Carbon Dioxide By Mamata Mukhopadhyay
2. Supercritical Fluid Methods and Protocols By Tony Clifford (Publisher: Humana Press)
3. Supercritical Fluid Extraction and Its Use in Chromatographic Sample Preparation (Publisher: Springer, Netherlands)