Systemic Virology and Mycology
Teacher
Dr. Monali Priyadarsini Mishra
Category
Core Courses
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Code(Credit) : (3-2-0)
Course Objectives
- To learn opportunities in the basic principles of medical microbiology and infectious disease.
- To study mechanisms of infectious disease transmission, principles of aseptic practice, and the role of the human body’s normal microflora.
- To understand the importance of pathogenic Virus and fungus in human disease with respect to infections of the respiratory tract, gastrointestinal tract, urinary tract, skin and soft tissue.
Learning Outcomes
- The course provides the conceptual basis for understanding pathogenic microorganisms and the mechanisms by which they cause disease in the human body.
- Know the methods used in studying viruses and fungi.
- Recall the relationship of this infection to symptoms, relapse and the accompanying pathology.
- Explain the methods of microorganism’s control, e.g. chemotherapy & vaccines. Solve problems in the context of this understanding.
Course Syllabus
Module-1
Structure, cultivation and properties of Viruses. Classification of Medically Important Viruses. Genetic material, Organ system involved, Transmission.
Replication of Viruses, Virus Host Interaction, Bacteriophage. Epidemiology, Pathogenesis, Treatment, Prevention and Control of Viral Diseases (DNA Viruses) Adenoviridae, Poxviridaes, Herpes viridaes, Epidemiology, Pathogenesis, Treatment, Prevention & Control of Viral Diseases (RNA Viruses) Orthomyxoviridae, Paramyxoviridae, Picornaviridae, Corona viridae, Rhabdoviridae, Retrovirida.
Practice:
- Demonstration of virus isolation techniques.
- Demonstration of cell and tissue culture techniques used for virus isolation
- Serological techniques used in diagnostic virology
Module-2
Mycology, Classification, Scope and medical importance of fungi. General Structure of Fungus and Yeast. Laboratory Methods of Fungal Isolation and Identification. Superficial and Cutaneous Mycoses. Subcutaneous Mycoses. Systemic Mycosis caused by Endemic Dimorphic Fungal Pathogens, Systemic Mycosis caused by Endemic Dimorphic Fungal Pathogens, Opportunistic Mycoses
Practice:
- Organization of laboratory – Mycology
- Preparation of different media, chemical and stain for fungus study
- Microscopic examination of saprophytic molds / Collection of agar plates for exposure
Module-3
Pathogenic Group of Fungi: Opportunistic pathogens, True pathogens: Blastomyces dermatitidis, Cooccidioidesimmitis, Paracocidioidesbrasiliensis, Histoplasma capsulatum. A. Dermatophytes: Mycrosporum (Hair, skin), Tricophytom (Skin, hair, nail), Epidermophyton (Skin, nail), Aspergillus spp. Dermatomycosis (Candida albicans, Cryptococcus neofornans)
Practice:
- Slide culture technique for Superficial infections
- Culture and identification of yeasts
- Processing of specimens in Mycology lab
Suggested References:
- Textbook of Microbiology- Ananthanarayan & Paniker (10th Ed)
- Medical Microbiology-by Fritz H. Kayser et al
- Fundamental medical mycology / Errol Reiss, H. Jean Shadomy, and G. Marshall Lyon III
- Essential Medical Microbiology- by Rajesh Bhatia (4th Ed)
- Clinical Microbiology Procedures Handbook- by Amy L. Leber (4th Ed)
- The short text book of medical microbiology- by Satis Gupte (10th Ed)
Session Plan
Session 1
Structure, cultivation and properties of Viruses
Video:https://www.youtube.com/watch?v=lADC0C_WeH0
https://www.youtube.com/watch?v=WnWr12lxRO4
https://www.youtube.com/watch?v=WTe5Dw8vTSg
Presentation: https://www.slideshare.net/RIZWANABBAS3/viruses-structure-classification-and-characteristics
Session 2
Classification of Medically Important Viruses
Video: https://www.youtube.com/watch?v=o8Mrun85Q3U
Presentation: https://www.slideshare.net/doctorrao/medical-virology-introduction
Session 3
Genetic material
Video: https://www.youtube.com/watch?v=l-9iUpFGbxE
Presentation: https://www.slideshare.net/MarwanAlhalabi/genetic-material-66443541
Session 4
Organ system involved in virus infection
Video:https://www.youtube.com/watch?v=x4c6hBXU8a4
Presentation: https://www.slideshare.net/doctorrao/viral-infections-in-humans-basics
Session 5
Transmission
Video: https://www.youtube.com/watch?v=xjcsrU-ZmgY
Presentation: https://www.slideshare.net/gurya87/virus-transmission
Session 6
Session 7
Session 8
Bacteriophage
Video: https://www.youtube.com/watch?v=1JthYa5yevM
https://www.youtube.com/watch?v=nK2cirOqQS8
Presentation: https://www.slideshare.net/doctorrao/bacteriophages
Session 9
Epidemiology, Pathogenesis, Treatment, Prevention and Control of Viral Diseases (DNA Viruses): Adenoviridae
Video:https://www.youtube.com/watch?v=LmAeBU9uVQk
Presentation:https://www.slideshare.net/doctorrao/adenovirus-7940533
Session 10
Poxviridae
Video: https://www.youtube.com/watch?v=QGSpYWfb1b8
Presentation:https://www.slideshare.net/delmornavida/poxviruses-31035228
Session 11
Herpes viridae
Video:https://www.youtube.com/watch?v=u3We3ItXjOY
https://www.youtube.com/watch?v=IOUnXeqNyMs&t=1s
Presentation:https://www.slideshare.net/HugoSousa1/herpesviruses
Session 12
Epidemiology, Pathogenesis, Treatment, Prevention & Control of Viral Diseases (RNA Viruses) :Orthomyxoviridae
Video: https://www.youtube.com/watch?v=85R-6O6rrgw
Presentation: https://www.slideshare.net/victorabdu/orthomyxoviridae-2
Session 13
paramyxoviridae
Video: https://www.youtube.com/watch?v=1CRq9I3v5Ts
https://www.youtube.com/watch?v=s3ACcn8IWFc
Presentation: https://www.slideshare.net/doctorrao/paramyxoviridiae
Session 14
Picornaviridae
Video: https://www.youtube.com/watch?v=ycOXWGr5Dag
Presentation: https://www.slideshare.net/praveen4777/picornaviruses-060811
Session 15
Session 16
Rhabdoviridae
Video: https://www.youtube.com/watch?v=8ZSCtJvPUiE
Presentation: https://www.slideshare.net/deshkar/rhabdovirus-lecture
Session 17
Retroviridae
Video: https://www.youtube.com/watch?v=EGfMRVBDxaY
https://www.youtube.com/watch?v=5g1ijpBI6Dk
Presentation: https://www.slideshare.net/ankit_2408/human-retroviruses
Practice:
Demonstration of cell and tissue culture techniques used for virus isolation
Practice:
Serological techniques used in diagnostic virology
Session 18
Mycology, Classification, Scope
Video: https://www.youtube.com/watch?v=3OuQWRWIUEE
Presentation: https://www.slideshare.net/mukhitkazi/medical-mycology
Session 19
medical importance of fungi
Video: https://www.youtube.com/watch?v=f7QxqWEKoGs
Presentatuion: https://www.slideshare.net/mgetso/introduction-to-medical-mycology
Session 20
General Structure of Fungus and Yeast
Video : https://www.youtube.com/watch?v=QcuPKy_Slng
https://www.youtube.com/watch?v=XY6akiB-IYk
Presentation: https://www.slideshare.net/khushbo0/fungi-44385809
Session 21
Laboratory Methods of Fungal Isolation and Identification
Video: https://www.youtube.com/watch?v=GV840R4Pyi4&t=178s
Presentation: https://www.slideshare.net/NaveenParvathareddy/lab-diagnosis-of-fungal-infections
Session 22
Superficial and Cutaneous Mycoses
Video: https://www.youtube.com/watch?v=GV840R4Pyi4&t=353s
Presentation: https://www.slideshare.net/MizanRahman9/superficial-mycoses-63214580
Video: https://www.youtube.com/watch?v=GdgRPj7zmwA
Presentation: https://www.slideshare.net/PrincessAlenAguilar/trichophyton-and-epidermophyton
Session 23
Subcutaneous Mycoses
Video: https://www.youtube.com/watch?v=gvcDExVxWv0
Presentation: https://www.slideshare.net/RakeshPrasadSah/classification-of-mycoses-by-dr-rakesh-prasad-sah-116628023
Session 24
Systemic Mycosis caused by Endemic Dimorphic Fungal Pathogens
Video: https://www.youtube.com/watch?v=bLpxM_8i3SA
Presentation: https://www.slideshare.net/thilgar/systemic-mycosis-36158507
Session 25
Opportunistic Mycoses
Video: https://www.youtube.com/watch?v=-Rt4AgGm8gw
https://www.youtube.com/watch?v=L6sZUk0kVtY
Presentation: https://www.slideshare.net/doctorrao/opportunistic-fungal-infections
Practice:
Preparation of different media, chemical and stain for fungus study
Practice:
Microscopic examination of saprophytic molds / Collection of agar plates for exposure
Session 26
Pathogenic Group of Fungi: Opportunistic pathogens
Video: https://www.youtube.com/watch?v=L6sZUk0kVtY&t=651s
Presentation: https://www.slideshare.net/doctorrao/opportunistic-fungal-infections
Session 27
True pathogens: Blastomyces dermatitidis
Video: https://www.youtube.com/watch?v=eMRsqejBZWo
Presentation: https://cmr.asm.org/content/23/2/367
Session 28
Cooccidioidesimmitis
Session 29
Paracocidioides brasiliensis
Session 30
Histoplasma capsulatum
Video: https://www.youtube.com/watch?v=T8T9Sk8B1_g
Presentation: https://www.slideshare.net/shahmanthan24/histoplasmois-mycology-epidemiology-laboratory-diagnosis
Session 31
A. Dermatophytes: Mycrosporum (Hair, skin)
Video: https://www.youtube.com/watch?v=oAIVjAAhtBw
Presentation: https://www.slideshare.net/rubaiyakabir/dermatophytes-69013431
Session 32
Tricophyton (Skin, hair, nail)
Video:https://www.youtube.com/watch?v=fU0fCyJ4x4c
https://www.youtube.com/watch?v=hrH6pJXC0vE
https://www.youtube.com/watch?v=lwKgAMT5BWw
Presentation:https://www.slideshare.net/AnkurVashishtha4/dermatophytes-147606801
Session 33
Epidermophyton (Skin, nail)
Video: https://www.youtube.com/watch?v=Kh4eS1CX6gA
Presentation: https://www.slideshare.net/PrincessAlenAguilar/trichophyton-and-epidermophyton
Session 34
Aspergillus spp
Video: https://www.youtube.com/watch?v=N1UUazCiL78
Video: https://www.youtube.com/watch?v=NDrbHSIzcLI
Presentation: https://www.slideshare.net/rajhaviknesh/aspergillosis-eassignm
Session 35
Dermatomycosis (Candida albicans, Cryptococcus neofornans)
Video: https://www.youtube.com/watch?v=OSlFOSLPFlM
Video: https://www.youtube.com/watch?v=4G1nFqPPRAA
Prfesentation: https://www.slideshare.net/mafambakhathu/candidiasis-46179900
Video: https://www.youtube.com/watch?v=7SL-0J_zCT8
Video: https://www.youtube.com/watch?v=Y8bd8TPFO0A
Presentation: https://www.slideshare.net/doctorrao/cryptococcus-neoformans-9872069
Practice:
Slide culture technique for Superficial infections
Case Studies
Case Studies
Our Main Teachers
Dr. Monali Priyadarsini Mishra is currently working as Assistant Professor in CUTM, Bhubaneswar. Her area of research belongs to “Clinical and Applied Microbiology, Biotechnology, Phytochemistry”. She has completed her Ph.D. from Siksha ‘O’ Anusandhan University, Bhubaneswar. She has authored several scientific papers in peer-reviewed international journals. Currently, She is working on some biologically active antimicrobial […]