Systemic Virology and Mycology

Teacher

Dr. Monali Priyadarsini Mishra

Category

Core Courses

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Course Name :CUTM1735-Systemic Virology & Mycology

 

Code(Credit) : (3-2-0)

Course Objectives

  • To learn opportunities in the basic principles of medical microbiology and infectious disease.
  • To study mechanisms of infectious disease transmission, principles of aseptic practice, and the role of the human body’s normal microflora.
  • To understand the importance of pathogenic Virus and fungus in human disease with respect to infections of the respiratory tract, gastrointestinal tract, urinary tract, skin and soft tissue.

Learning Outcomes

  • The course provides the conceptual basis for understanding pathogenic microorganisms and the mechanisms by which they cause disease in the human body.
  • Know the methods used in studying viruses and fungi.
  • Recall the relationship of this infection to symptoms, relapse and the accompanying pathology.
  • Explain the methods of microorganism’s control, e.g. chemotherapy & vaccines. Solve problems in the context of this understanding.

Course Syllabus

Module-1 

Structure, cultivation and properties of Viruses. Classification of Medically Important Viruses. Genetic material, Organ system involved, Transmission.

Replication of Viruses, Virus Host Interaction, Bacteriophage. Epidemiology, Pathogenesis, Treatment, Prevention and Control of Viral Diseases (DNA Viruses) Adenoviridae, Poxviridaes, Herpes viridaes, Epidemiology, Pathogenesis, Treatment, Prevention & Control of Viral Diseases (RNA Viruses) Orthomyxoviridae, Paramyxoviridae, Picornaviridae, Corona viridae, Rhabdoviridae, Retrovirida.

Practice:

  1. Demonstration of virus isolation techniques.
  2. Demonstration of cell and tissue culture techniques used for virus isolation
  3. Serological techniques used in diagnostic virology

Module-2 

Mycology, Classification, Scope and medical importance of fungi. General Structure of Fungus and Yeast. Laboratory Methods of Fungal Isolation and Identification. Superficial and Cutaneous Mycoses. Subcutaneous Mycoses. Systemic Mycosis caused by Endemic Dimorphic Fungal Pathogens, Systemic Mycosis caused by Endemic Dimorphic Fungal Pathogens, Opportunistic Mycoses

Practice:

  1. Organization of laboratory – Mycology
  2. Preparation of different media, chemical and stain for fungus study
  3. Microscopic examination of saprophytic molds / Collection of agar plates for exposure

Module-3 

Pathogenic Group of Fungi: Opportunistic pathogens, True pathogens: Blastomyces dermatitidis, Cooccidioidesimmitis, Paracocidioidesbrasiliensis, Histoplasma capsulatum. A. Dermatophytes: Mycrosporum (Hair, skin), Tricophytom (Skin, hair, nail), Epidermophyton (Skin, nail), Aspergillus spp. Dermatomycosis (Candida albicans, Cryptococcus neofornans)

Practice:

  1. Slide culture technique for Superficial infections
  2. Culture and identification of yeasts
  3. Processing of specimens in Mycology lab

Suggested References:

  1. Textbook of Microbiology- Ananthanarayan & Paniker (10th Ed)
  2. Medical Microbiology-by Fritz H. Kayser et al
  3. Fundamental medical mycology / Errol Reiss, H. Jean Shadomy, and G. Marshall Lyon III
  4. Essential Medical Microbiology- by Rajesh Bhatia (4th Ed)
  5. Clinical Microbiology Procedures Handbook- by Amy L. Leber (4th Ed)
  6. The short text book of medical microbiology- by Satis Gupte (10th Ed)

Session Plan

Session 1

Session 2

Session 3

Session 4

Session 5

Session 6

Session 7

Session 8

Session 9

Epidemiology, Pathogenesis, Treatment, Prevention and Control of Viral Diseases (DNA Viruses):  Adenoviridae

Video:https://www.youtube.com/watch?v=LmAeBU9uVQk

Presentation:https://www.slideshare.net/doctorrao/adenovirus-7940533

Session 10

Session 11

Session 12

Epidemiology, Pathogenesis, Treatment, Prevention & Control of Viral Diseases (RNA Viruses) :Orthomyxoviridae

Video: https://www.youtube.com/watch?v=85R-6O6rrgw

Presentation: https://www.slideshare.net/victorabdu/orthomyxoviridae-2

Session 13

Session 14

Session 15

Session 16

Session 17

Practice:

Demonstration of cell and tissue culture techniques used for virus isolation

https://www.youtube.com/watch?v=zAd2RsWLgeo

https://www.youtube.com/watch?v=-QoDXj-hTwA

Practice:

Serological techniques used in diagnostic virology

https://www.youtube.com/watch?v=wGZU1-HfsR4

https://www.youtube.com/watch?v=nnq8hnUs5oQ

Session 18

Session 19

Session 20

Session 21

Session 22

Session 23

Session 24

Systemic Mycosis caused by Endemic Dimorphic Fungal Pathogens

Video: https://www.youtube.com/watch?v=bLpxM_8i3SA

Presentation: https://www.slideshare.net/thilgar/systemic-mycosis-36158507

Session 25

Practice:

Preparation of different media, chemical and stain for fungus study

https://www.youtube.com/watch?v=_ngtLifA6hc

https://www.youtube.com/watch?v=OMIF1Elr9i4&t=10s

Practice:

Microscopic examination of saprophytic molds / Collection of agar plates for exposure

https://www.youtube.com/watch?v=HmXF1o0mo-k

Session 26

Session 27

True pathogens: Blastomyces dermatitidis

Video: https://www.youtube.com/watch?v=eMRsqejBZWo

Presentation: https://cmr.asm.org/content/23/2/367

Session 28

Session 29

Paracocidioides brasiliensis

https://www.youtube.com/watch?v=u-IR6LKsk-U

Session 30

Session 31

Session 32

Session 33

Session 34

Session 35

Practice:

Slide culture technique for Superficial infections

https://www.youtube.com/watch?v=wcNnfW5eI1o

Practice:

Culture and identification of yeasts

https://www.youtube.com/watch?v=affbOWy0fGQ

Practice:

Processing of specimens in Mycology lab

https://www.youtube.com/watch?v=4J1T2mQMxXM

Case Studies

Case Studies

Our Main Teachers

Dr. Monali Priyadarsini Mishra is currently working as Assistant Professor in CUTM, Bhubaneswar. Her area of research belongs to “Clinical and Applied Microbiology, Biotechnology, Phytochemistry”. She has completed her Ph.D. from Siksha ‘O’ Anusandhan University, Bhubaneswar. She has authored several scientific papers in peer-reviewed international journals. Currently, She is working on some biologically active antimicrobial […]