Pharmacology II practical

Teacher

Ashirbad Nanda

Category

Core Courses

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Course Name : Pharmacology II (Practical)

Code(Credit) : BPHL3102(0-2-0)

Course Objectives

This subject is intended to impart the effects of different drugs on animals.

Course outcome

CO Statement Mapping with POs
CO-1 Understand the mechanism of drug action and its relevance in the treatment of different diseases

 

 PO1, PO2, PO6
CO-2 Demonstrate isolation of different organs/tissues from the laboratory animals by simulated experiments

 

PO1, PO6, PO8
CO-3 Demonstrate the various receptor actions using isolated tissue preparation PO1, PO8, PO9
CO-4 Appreciate correlation of pharmacology with related medical sciences PO1, PO3, PO8
CO-5 To get knowledge for the practical skills required to conduct the preclinical toxicity studies. PO1, PO6, PO8

Course Syllabus

1. Introduction to in-vitro pharmacology and physiological salt solutions.
2. Effect of drugs on isolated frog heart.
3. Effect of drugs on blood pressure and heart rate of dog.
4. Study of diuretic activity of drugs using rats/mice.
5. DRC of acetylcholine using frog rectus abdominis muscle.
6. Effect of physostigmine and atropine on DRC of acetylcholine using frog rectus
abdominis muscle and rat ileum respectively.
7. Bioassay of histamine using guinea pig ileum by matching method.
8. Bioassay of oxytocin using rat uterine horn by interpolation method.
9. Bioassay of serotonin using rat fundus strip by three point bioassay.
10. Bioassay of acetylcholine using rat ileum/colon by four point bioassay.
11. Determination of PA 2 value of prazosin using rat anococcygeus muscle (by
Schilds plot method).
12. Determination of PD 2 value using guinea pig ileum.
13. Effect of spasmogens and spasmolytics using rabbit jejunum.
14. Anti-inflammatory activity of drugs using carrageenan induced paw-edema
model.
15. Analgesic activity of drug using central and peripheral methods
Note: All laboratory techniques and animal experiments are demonstrated by simulated
experiments by softwares and videos
Recommended Books
1. Rang H. P., Dale M. M., Ritter J. M., Flower R. J., Rang and Dale’s Pharmacology,
Churchil Livingstone Elsevier
2. Katzung B. G., Masters S. B., Trevor A. J., Basic and clinical pharmacology, Tata Mc
Graw-Hill.
3. Goodman and Gilman’s, The Pharmacological Basis of Therapeutics
4. Marry Anne K. K., Lloyd Yee Y., Brian K. A., Robbin L.C., Joseph G. B., Wayne A.
K., Bradley R.W., Applied Therapeutics, The Clinical use of Drugs, The Point
Lippincott Williams & Wilkins.
5. Mycek M.J, Gelnet S.B and Perper M.M. Lippincott’s Illustrated Reviews-
Pharmacology.
6. K.D.Tripathi. Essentials of Medical Pharmacology, , JAYPEE Brothers Medical
Publishers (P) Ltd, New Delhi.
7. Sharma H. L., Sharma K. K., Principles of Pharmacology, Paras medical publisher
8. Modern Pharmacology with clinical Applications, by Charles R.Craig& Robert.
9. Ghosh MN. Fundamentals of Experimental Pharmacology. Hilton & Company,
Kolkata.
10. Kulkarni SK. Handbook of experimental pharmacology. Vallabh Prakashan.

EXPERIMENT 1

Introduction to in vivo Pharmacology

Introduction to in vivo pharmacology

EXPERIMENT 2

EXPERIMENT 3

Preparation of Physiological Salt Solution

physiological salt solution

EXPERIMENT 4

DRC of acetyl choline using frog rectus abdominus  muscle

DRC of Acetyl choline

EXPERIMENT 5

DRC of Epinephrine using frog rectus abdominus  muscle

DRC of Acetyl choline

EXPERIMENT 6

DRC of Acetyl choline

DRC of Propanolol using frog rectus abdominus  muscle

EXPERIMENT 7

DRC of Acetyl choline

Effect of cilliary motility

EXPERIMENT 8

DRC of Acetyl choline

Myotic effect on rabbit eye

EXPERIMENT 9

DRC of Acetyl choline

Effect of drug on Blood Pressure and Heart rate

EXPERIMENT 10

DRC of Acetyl choline

Bio Assay of Histamine

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