FORENSIC BIOLOGY, SEROLOGY AND DNA FINGERPRINTING – CUTM2316

Teacher

Dr. Naga Jogayya K

Category

Core Courses

Course Attendees

Still no participant

Course Reviews

Still no reviews

Credit Course: (3+2+0)

COURSE OBJECTIVES:

  • To Learn about Different body fluids, their detection and forensic significance.
  • To Learn about the differences between human and animal hair.
  • To Learn about the forensic microbiology and Microbial forensics.
  • The study basics of immunology and overview of genetic inheritance.
  • To Learn about DNA fingerprinting and its application in forensics

Course Outcome:

After successful completion of the course, the students will be able to :

  • Distinguish between hair samples from different sources and also understand the significance of biological evidences.
  • Identify and analyse different body fluids found in a crime scene.
  • Demonstrate the significance of microbes in forensic sciences, and overview of immunology.
  • Gain skills in DNA extraction and understand the significance of DNA Profiling in Forensic Sciences

SYLLABUS

Unit I: Biological Evidence

Nature and importance of biological evidence. Significance of hair evidence. Transfer, persistence and recovery of hair evidence. Structure of human hair. Comparison of hair samples. Morphology and Biochemistry of human hair. Comparison of human and animal hair. Diatoms and their forensic significance. Palynology and limnology.

Unit II: Forensic Microbiology

Introduction of Microbiology, Types of microbes, Microbial organisms of forensic significance. Role Microbial DNA in Crime Scene Investigation, Biological warfare Agents, Microbial Bioterrorism.

Unit III: Introduction to Serology

Overview of basic Immunology, Cellular antigens, Antibodies. Blood and its components, Body Fluids, Types of Body fluids, Composition, Functions and Circulation of Body Fluids. Collection and preservation of body fluids.

Unit IV: Forensic Examination of Body fluids

 Determination of blood groups. Antigens and antibodies. Forensic characterization of bloodstains. Typing of dried stains. Morphology of spermatozoa. Tests for identification of semen, saliva, sweat, urine, vaginal secretions, faecal matter. Individualization on the basis of semen examination. Forensic significance of body fluids.

 Unit V: Blood stain pattern and Genetic marker analysis

 Fundamentals of Bloodstain characteristics. Impact bloodstain patterns. Cast-off bloodstain patterns. Projected bloodstain patterns. Contact bloodstain patterns. Blood trails. Bloodstain drying times. Crime scene reconstruction with the aid of bloodstain pattern analysis.

 Unit VI: Basics of DNA & DNA Fingerprinting

 Structure of DNA and RNA, Overview of DNA replication, transcription and translation. History of DNA fingerprinting. Introduction to mitochondrial DNA and its forensic importance. Procedure for collection and preservation of biological sample for DNA analysis.

 Unit VII: DNA Typing & Forensic Significance

 Introduction to Polymerase Chain Reaction and its applications. Genes and DNA markers in forensic DNA analysis - STR, VNTR, SNP.

Techniques of DNA isolation and its quantitation. Introduction to RFLP.

Overview of modern trends in DNA Fingerprinting. Sexual assault investigations and Parentage Testing.

Session 1:

Nature and importance of biological evidence. Significance of hair evidence

Transfer, persistence and recovery of hair evidence

Hair Evidence

http://what-when-how.com/forensic-sciences/hair-transfer-persistence-and-recovery/

Session 2:

Structure of human hair

hair-info

Session 3:

Comparison of hair samples

Comparison of human and animal hair

https://www.differencebetween.com/difference-between-human-and-vs-animal-hair/

https://pubmed.ncbi.nlm.nih.gov/36643134/

 

Session 4:

Morphology and Biochemistry of human hair

2010AdvBiochemEngBiotechnol1

Session 5:

Diatoms and their forensic significance

https://www.slideshare.net/AnikeshBanik1/diatoms-in-forensic-science

Session 10: 

Introduction of Microbiology

https://byjus.com/biology/microbiology/

Session 12:

Microbial organisms of Forensic Significance

https://youtu.be/VOJ_aJvARsA?t=470

Session 13:

Role Microbial DNA in Crime Scene Investigation

https://youtu.be/WT93IM1iLDM

Session 14:

Biological warfare Agents

Biological warfare

Session 15:

Microbial Bioterrorism

https://www.publish.csiro.au/ma/pdf/MA20031

Session 17:

Cellular antigens

https://www.youtube.com/watch?v=jWuMaXf6KZE

 

Antigen

Session 19:

Blood and its components

https://www.youtube.com/watch?v=XUUtdCIiWhU

Session 21:

Types of Body fluids

https://www.youtube.com/watch?v=tC2gMDfzYA8

Session 23:

Functions and Circulation of Body Fluids

https://www.youtube.com/watch?v=R3OQ7_rSnhc

Session 25:

Determination of blood groups

https://byjus.com/biology/blood-group-test/

Session 26:

Antigens and antibodies

https://www.youtube.com/watch?v=jWuMaXf6KZE

Session 27:

Forensic characterization of bloodstains

https://www.forensicsciencesimplified.org/blood/principles.html

Session 30

Tests for identification of semen, saliva, sweat, urine, vaginal secretions, faecal matter

Session 31

Individualization on the basis of semen examination.

Forensic significance of body fluids.

Session 32

Fundamentals of Bloodstain characteristics

Session 33

Impact bloodstain patterns

Session 34

Cast-off bloodstain patterns

Session 35

Projected bloodstain patterns

Session 36

Contact bloodstain patterns

Session 37

Blood trails

Session 38

Bloodstain drying times

Session 39

Crime scene reconstruction with the aid of bloodstain pattern analysis

Session 40

Structure of DNA and RNA

Session 41

Overview of DNA replication, transcription and translation

Session 42

History of DNA fingerprinting

Session 43

Introduction to mitochondrial DNA and its forensic importance

Session 44

Procedure for collection and preservation of biological sample for DNA analysis

Session 45

Introduction to Polymerase Chain Reaction and its applications

Session 46

Genes and DNA markers in forensic DNA analysis - STR, VNTR, SNP.

Session 47

Techniques of DNA isolation and its quantitation

Session 48

Introduction to RFLP

Session 49

Overview of modern trends in DNA Fingerprinting

Session 50

Sexual assault investigations and Parentage Testing.

Our Main Teachers

Dr. Naga Jogayya K

Assistant Professor & HOD
VIEW PROFILE