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On successful completion of the course, the students will be able to
UNIT- I - Basics of Instrumentation:
Electromagnetic radiations and their properties
General properties of electromagnetic radiations: Wave and Quantum mechanical properties Interaction of EMR with matter
Photoelectric Effect
De Broglie-Bohr Theory and derivation of equation
Heisenberg uncertainty principle
Plank’s Quantum theory
Davisson and Germer Experiment
Electronic spectra and molecular structure
UNIT- II - Spectroscopic Techniques:
Ultraviolet and visible spectroscopy: Instrumentation and Applications.
Infrared Spectroscopy: Molecular vibration, Theory of IR absorption, IR Sources and Instrumentation, FT-IR Applications.
Basics of Mass Spectroscopy
UNIT- III - Chromatography and Hyphenated Techniques:
Introduction to Chromatography: Theory of separation techniques
Types of chromatography and their Forensic Applications
Thin layer chromatography
High Performance Liquid Chromatography
Gas Chromatography
GC-MS, GC-HS
LC-MS, LC-MS/MS
UNIT- iV
General Principles of Biological / Biochemical Analysis
pH and Buffers, Physiological solution
Centrifugation Techniques
Basic principle of centrifugation and sedimentation
various types of centrifuges: Density Gradient, Preparative, Ultra centrifuge- Refrigerated Centrifuges. Analysis of sub-cellular fractions.
Microscopy
Basic principles of microscopy, Simple and Compound microscope
Study of different types of microscopes: Comparison microscope, Phase contrast microscope, Stereoscopic microscope, Polarizing microscope, Fluorescence microscopy, IR microscopy, Scanning Electron Microscope (SEM)
Reference Books:
1. D.A.Skoog, F.J.Holler and T.A.Neman, Harcoust Principles of Instrumental Analysis college publishers, Singapore
2. G.D.Christian and J.E.O’Reilly, Instrumental Analysis, Allyn and Bacon, Inc., Boston.
3. F.W.Fifield and D.Kealey, Principles and practice of Analytical Chemistry, International Textbook Company, London.
4. R.P.Bauman, Absorption Spectroscopy, John Wiely, New York.
5. M.Donhrow, Instrumental Methods in Analytical Chemistry; Their Principles and practice Vol.2, optical method, Pitaman, New York.
6. G.G.Guilbanlt, Practical Fluorenscence: Theory, Methods and Practice, Marcel Dekker, New York.
7. S.Udenfriend, Fluorescence Assay in Biology and Medicine, Academic Press, New York.
8. W.J.Price, Spectrochemical Analysis by Atomic Absorption, Hyden, London.
9. R.S.Alger, Electron Paramagnetic Resonance: Techniques and Applications, Interscience, New York.
10. Analytical Chemistry by open Learning, John Wiley & Sons, New York.
11. J.C.Giddings, Dynamics of Chromatography, Marcel Dekker, New York.
12. R.C.Grob, Modern Techniques of Gas Chromatography, Marcel Dekker, New York.
13. J.A.Dean, Chemical Separation Methods, Ban Nostrand Reinhold Co., New York.
14. R.E.Smith, Ion Chromatography Applications, C.R.C. Press, Inc., Boca Raton.
15. R.E.Smith, Supercritical Fluid Technology, C.R.C. Press, Inc., Boca Raton.
16. G.Zweig and J.R.Whitaker, Paper Chromatography and Electrophoresis, Academic Press, New York.
17. Safferstein: Forensic Science Handbook Vol. I, II, III.
18. Lee Honry: An Introduction to Forensic Science
19. Egon Stahl: Thin Layer Chromatography
Associate Professor at the School of Forensic Sciences, Centurion University of Technology and Management, Orrisa, Bhubaneshwar.