CUTM 2729 Instrumental Techniques

Teacher

Dr. Reena C. Jhamtani

Category

Core Courses

Course Attendees

Still no participant

Course Reviews

Still no reviews

Course Name: Instrumental Techniques

Code (Credit) : CUTM 2729 /FSMS SI-2 (4-0-0)

Course Objectives

  1. To help students learn theory and basics of instrumentation
  2. To help students learn Spectroscopic and chromatographic techniques
  3. To study the concepts of microscopy and centrifugation techniques.

Course Outcomes:

On successful completion of the course, the students will be able to

  1. Demonstrate the basic theories and instrumentation in the aspect of EMR interaction and structure of an atom.
  2. Understand the basic principles and instrumentation of UV, IR, and Mass Spectroscopy.
  3. Comprehend the concepts and instrumentation of chromatographic techniques
  4. Acquire knowledge of bio-chemical analysis along with microscopy and centrifugation techniques.
  5. Identify and implement the instrumental techniques learnt for analysis

Course Syllabus

UNIT- I - Basics of Instrumentation:

 Electromagnetic radiations and their properties
 General properties of electromagnetic radiations: Wave and Quantum mechanical properties Interaction of EMR with matter
 Photoelectric Effect
 De Broglie-Bohr Theory and derivation of equation
 Heisenberg uncertainty principle
 Plank’s Quantum theory
 Davisson and Germer Experiment
 Electronic spectra and molecular structure

UNIT- II -  Spectroscopic Techniques:

 Ultraviolet and visible spectroscopy: Instrumentation and Applications.
 Infrared Spectroscopy: Molecular vibration, Theory of IR absorption, IR Sources and Instrumentation, FT-IR Applications.
 Basics of Mass Spectroscopy

UNIT- III - Chromatography and Hyphenated Techniques:

 Introduction to Chromatography: Theory of separation techniques
 Types of chromatography and their Forensic Applications
 Thin layer chromatography
 High Performance Liquid Chromatography
 Gas Chromatography
 GC-MS, GC-HS
 LC-MS, LC-MS/MS

UNIT- iV
General Principles of Biological / Biochemical Analysis
 pH and Buffers, Physiological solution
Centrifugation Techniques
 Basic principle of centrifugation and sedimentation
 various types of centrifuges: Density Gradient, Preparative, Ultra centrifuge- Refrigerated Centrifuges. Analysis of sub-cellular fractions.
Microscopy
 Basic principles of microscopy, Simple and Compound microscope
 Study of different types of microscopes: Comparison microscope, Phase contrast microscope, Stereoscopic microscope, Polarizing microscope, Fluorescence microscopy, IR microscopy, Scanning Electron Microscope (SEM)

Reference Books:
1. D.A.Skoog, F.J.Holler and T.A.Neman, Harcoust Principles of Instrumental Analysis college publishers, Singapore
2. G.D.Christian and J.E.O’Reilly, Instrumental Analysis, Allyn and Bacon, Inc., Boston.
3. F.W.Fifield and D.Kealey, Principles and practice of Analytical Chemistry, International Textbook Company, London.
4. R.P.Bauman, Absorption Spectroscopy, John Wiely, New York.
5. M.Donhrow, Instrumental Methods in Analytical Chemistry; Their Principles and practice Vol.2, optical method, Pitaman, New York.
6. G.G.Guilbanlt, Practical Fluorenscence: Theory, Methods and Practice, Marcel Dekker, New York.
7. S.Udenfriend, Fluorescence Assay in Biology and Medicine, Academic Press, New York.
8. W.J.Price, Spectrochemical Analysis by Atomic Absorption, Hyden, London.
9. R.S.Alger, Electron Paramagnetic Resonance: Techniques and Applications, Interscience, New York.
10. Analytical Chemistry by open Learning, John Wiley & Sons, New York.
11. J.C.Giddings, Dynamics of Chromatography, Marcel Dekker, New York.
12. R.C.Grob, Modern Techniques of Gas Chromatography, Marcel Dekker, New York.
13. J.A.Dean, Chemical Separation Methods, Ban Nostrand Reinhold Co., New York.
14. R.E.Smith, Ion Chromatography Applications, C.R.C. Press, Inc., Boca Raton.
15. R.E.Smith, Supercritical Fluid Technology, C.R.C. Press, Inc., Boca Raton.
16. G.Zweig and J.R.Whitaker, Paper Chromatography and Electrophoresis, Academic Press, New York.
17. Safferstein: Forensic Science Handbook Vol. I, II, III.
18. Lee Honry: An Introduction to Forensic Science
19. Egon Stahl: Thin Layer Chromatography

Session Plan- Theory

Session 1: Electromagnetic radiations and its properties

Session 2: General properties of electromagnetic radiations: Wave and Quantum mechanics.

Session 3: Interaction of EMR with matter

Session 4: Photoelectric Effect

Session 5: De Broglie-Bohr Theory and Derivation of Equation

Session 6: Heisenberg Uncertainty Principle

Session 7: Planks Quantum Theory

Session 8: Davission and Germers Experiment

Session 9: Electronic spectra and molecular structure

Session 10: Absorption, Emission and  Transmission spectroscopy

Session 11: Ultraviolet and visible spectroscopy: Instrumentation and Applications.

Session 12: Infrared Spectroscopy: Molecular vibration, Theory of IR absorption, FTIR

Session 13: Basics of Mass Spectroscopy

Session 14: Introduction to Chromatography

Session 15: Types of Chromatography and its Forensic applications

Session 16: Thin Layer Chromatography

Session 17: High Performance Liquid Chromatography

Session 18: Gas Chromatography

Session 19: GC-MS

Session 21: LC – MS, LC – MS /MS

Session 20: GC –HS

Session 22: pH and Buffers, Physiological solution

Session 23: Basic principle of centrifugation and sedimentation

Session 24: Various types of centrifuges: Density Gradient Centrifugation

Session 25: Analysis of sub-cellular fractions

Session 26: Preparative Centrifugation, Ultra centrifuge- Refrigerated Centrifuges

Session 27: Basic principles of microscopy

Session 28: Simple and Compound microscope

Session 29: Study of different types of microscopes: Comparison microscope

Session 30: Phase contrast microscope, Stereoscopic microscope, Polarizing microscope

Session 31: Fluorescence microscopy, IR microscopy

Session 32: Scanning Electron Microscope (SEM)

Our Main Teachers

Associate Professor at the School of Forensic Sciences, Centurion University of Technology and Management, Orrisa, Bhubaneshwar.