Domaindomain-nutraceuticals

Domain Track: Nutraceuticals

Syllabus

Faculty: Preetha Bhadra

Domain Track Title : Nutraceuticals

Track Total Credits ( T-P-P): 3-9-17

Courses Division( list all divisions):

Track courses (3-6-0)

Introduction to Nutraceutical (1+2)
Functional Food (1+2)
Nutrigenetics (1+2)

Project Courses (0-3-6)

Development of Personalized Food and Medicine (0-1-2)
Development of Biopesticides and Biofertilizers (0-1-2)
Development of Immune Boosters (0-1-2)

AELP Linked Project Courses (0-0-11) (For Agriculture Students only)

To develop personalized food and beverages rich in Antioxidants and Polyphenols
To Develop low-cost omega-3 capsules from fish oil that can reduce inflammation and improve hypertriglyceridemia
To develop immune boosters
To develop energy bars
Development and Application of different pesticides and fertilizers in Organic farm
Development and Application of pesticides in fishes
Development and Application of drug designing in lab
Products/Patents/Publications

Domain Track Objectives:

To study the advantages of functional foods over conventional Medicine to avoid potential side-effects
To Study about dietary supplements
To Study about food gene interface
To distinguish between food, functional food, and supplements
To learn about Drug Designing,
To learn about detailed Protein structure, pharmacophore, basics of Docking and Discovery studio and application in Agriculture, Fisheries and others Sciences

Domain Track Learning Outcomes:

To understand Functional foods and their effects on human health
To understand the role of antioxidants, polyphenols, omega-3 fatty acids, to prevent different physiological disorders.
To Understand the importance of personalized food with respect to nutrigenetics.
To be able to design a drug molecule and identify its applications
To be able to analyze nanomaterials and use it as Bio-Pesticides and Biofertilizers,
To be able to design a drug molecule and identify its applications.
To be able to illustrate the structure of Protein, Pharmacophores

Domain Syllabus:

Projects and Products Validation: (3+17) (AELP linked)

Develop personalized food and beverages rich in Antioxidants and Polyphenols
Develop low-cost omega-3 capsules from fish oil that can reduce inflammation and improve hypertriglyceridemia
Develop immune boosters
Develop energy bars
Extraction of Antioxidants and Polyphenols from different sources. (eg: Ramie, Dragon Fruit, Berries)

Subject 1: Introduction to Nutraceutical (1+2)
Theory:
Organizational elements, classification of nutraceuticals, dietary supplements, fortified foods, functional foods, and Phyto-nutraceuticals. The scope involved in the industry, Indian, and global scenario, Food Chemistry, Preservatives, food colors.
Practicals:

Milk Analysis: Determination of fat, acidity, lactose, protein, adulterants
Analysis of water: Collection of the sample, preliminary examination, Total soluble solids, Determination of total hardness, chlorides, dissolved oxygen
Analysis of fats: acid value, saponification value, iodine number, antioxidants
Analysis of foods: Determination of reducing and non reducing sugar, protein, determination of ash/total protein/moisture in dietary fibers
Anti-nutritional Factors: Trypsin inhibitor, Phytic acid, Antifungal activity of cystatin
Vitamins: Ascorbic acid by colorimetric method, estimation of Vit A/E
Pigments: Curcumin, chlorophyll, carotene

Subject 2: Functional Food (1+2)
Theory:

Prebiotics- Definition, chemistry, sources, metabolism and bioavailability, the effect of processing, physiological effects, effects on human health, and potential applications in risk reduction of diseases. perspective for food applications for the – Non-digestible carbohydrates/oligosaccharides, Dietary fiber, Resistant starch, Gums.
Probiotics- Taxonomy and important features of probiotic microorganisms. Health effects of probiotics including mechanism of action. Probiotics in various foods: fermented milk products, non-milk products, etc. Quality Assurance of probiotics and safety.
Drug Discovery - Issues, Target and Lead Identification, Drug And Databases, Drug Properties, Drug Solubility/permeability,
ADME, Molecular Modelling, Quantitative Structure-Activity Relationship, Target-based drug design, Docking.
Pharmacokinetics/ pharmacodynamics, Discussion on Molecular Drug Designing, Targeted drug designing, Application

Practicals:

Extraction Techniques: Soxhlet Extraction, Cold Press Extraction, Super Critical Fluid Extraction,
CO2 Extraction
Isolation and Purification of Phytochemicals: TLC, Paper Chrom, Column Chrom, HPLC, Analysis of Aflatoxin
Validation of Phytochemicals: Using different microorganisms and mammalian cell lines

Subject 3: Nutrigenetics (1+2)
Theory:
Introduction to Nutrigenetics, Nutrigenomics, Concept of Personalized food with special references to athletes. Introduction to different types of public domain databases, data mining strategies, primer designing.
Practical:

m-RNA extraction from treated mammalian cell line
Preparation of c-DNA
Study of Gene expression in Real Time PCR
Electrophoresis

Domain Books:

Introduction to Nutraceuticals:

Giuseppe Mazza; Functional Foods: Biochemical and Processing Aspects, Volume 1; CRC Press
Robert E.C. Wildman; Handbook of Nutraceuticals and Functional Foods, Second Edition; CRC Press
Massimo Maffei; Dietary Supplements of Plant Origin; CRC Press
Fereidoon Sahidi, Deepthi K. Weerasinghe; Nutraceutical Beverages, Chemistry, Nutrition and Health Effects; American Chemical Society

Functional Foods:

Ronald R. Watson; Vegetables, Fruits, and Herbs in Health Promotion; CRC Press
Fruit and Cereal Bioactives: Sources, Chemistry and Applications; ÖzlemTokusoglu; Clifford Hall III; CRC Press
Susan Sungsoo Cho, Mark L. Dreher; Marcel; Dekker Handbook of Dietary Fibre

Nutrigenetics:

Principles of Nutrigenetics and Nutrigenomics - 1st Edition. Print Book & E-Book. ISBN 9780128045725, 9780128045879.
Nutrigenetics: Applying the Science of Personal Nutrition. Academic Press; Reprint edition (19 August 2016).
Nutrigenetics and Nutrigenomics. World Review of Nutrition and Dietetics, Vol. 93

Development of Personalized Food and Medicine
Textbook of Personalized Medicine. Authors: Jain, K. K. Springer Publication
Development of Biopesticides and Biofertilizers
Biofertilizers and Biopesticides Paperback – 29 June 2019 by Krishnendu Acharya (Author), Surjit Sen (Author), Manjula Rai (Author)
Biofertilizer and Biopesticide Hardcover – 1 March 2011, by Shalini Suri (Author)
Development of Immune Boosters
Immune System: How To Boost The Immune System Naturally by Elizabeth Grace

Session Plan: 1.1.1a.

Organizational elements

Organization of Elements
https://www.youtube.com/watch?v=xyQY8a-ng6g

Session Plan: 1.1.1b.

Classification of nutraceuticals

classification of nutraceuticals
https://www.youtube.com/watch?v=hqP_cGN7_EU

Session Plan: 1.1.1c.

Dietary supplements, fortified foods

dietary supplements
https://www.youtube.com/watch?v=KpXeWl2p5KM

Session Plan: 1.1.1d.

Phyto-nutraceuticals

Phyto-nutraceuticals
https://www.youtube.com/watch?v=HJ1jU4WIhBU

Session Plan: 1.1.1e.

The scope involved in the industry

Nutraceuticals Market_Global Forecast

Session Plan: 1.1.1f.

Indian, and global scenario

Nutraceuticals Market in India

Session Plan: 1.1.1g.

Food Chemistry

Food chemistry
https://www.youtube.com/watch?v=yg-fcTyUGjc

Session Plan: 1.1.1h.

Preservatives

preservatives
https://www.youtube.com/watch?v=uNKq9iIH_oE

Session Plan: 1.1.1i.

Food colors

Food colours
https://www.youtube.com/watch?v=ExXC3ZHWLmA

Session Plan: 1.1.2a.

Milk Analysis: Determination of fat, acidity, lactose, protein, adulterants
https://www.youtube.com/watch?v=b00ZDrfyaIk

Session Plan: 1.1.2b.

Analysis of water: Collection of the sample, preliminary examination, Total soluble solids, Determination of total hardness, chlorides, dissolved oxygen
https://www.youtube.com/watch?v=Ewe7i1D9lSQ
https://www.youtube.com/results?search_query=water+analysis

Session Plan: 1.1.2c.

Analysis of fats: acid value, saponification value, iodine number, antioxidants
https://www.youtube.com/watch?v=1a4Jy6JOjiY

Session Plan: 1.1.2d.

Analysis of foods: Determination of reducing and non-reducing sugar, protein, determination of ash/total protein/moisture in dietary fibers
https://www.youtube.com/watch?v=QacQmS3aaTI

Session Plan: 1.1.2e.

Anti-nutritional Factors: Trypsin inhibitor, Phytic acid, Antifungal activity of cystatin
https://www.youtube.com/watch?v=sLP8dcnWnJg

Session Plan: 1.1.2f.

Vitamins: Ascorbic acid by colorimetric method, estimation of Vit A/E
https://www.youtube.com/watch?v=r9en4aqkgL0

Session Plan: 1.2.1a.

Probiotics &Prebiotics-Definition, chemistry, source

New Scientific Paradigms for Probiotics and Prebiotics https://www.youtube.com/watch?v=fJWFv0REa1A
https://www.youtube.com/watch?v=0z47wLZ4-O4
https://www.youtube.com/watch?v=xjxYauok94Q

Session Plan: 1.2.1b.

Effect of processing, physiological effects

Functional food
https://www.youtube.com/watch?v=BHiJ30Om50E

Session Plan: 1.2.1c.

Perspective for food applications for the – Non-digestible carbohydrates/ oligosaccharides, Dietary fiber, Resistant starch, Gums

Role of different Probiotic and Prebiotics

Session Plan: 1.2.1d.

Probiotics- Taxonomy and important features of probiotic microorganisms

Probiotic Introduction

https://www.youtube.com/watch?v=OzTJlmiGS8I

Session Plan: 1.2.1e.

Health effects of probiotics including mechanism of action

Role of Functional Food
https://www.youtube.com/watch?v=ZnRwbDWz2ek

Session Plan: 1.2.1f.

Mechanism of Action

Mechanism of action of Prebiotics
Mechanism of action_research article

Session Plan: 1.2.1g.

Immunity and Nutraceuticals

ROLE OF OTHER PREBIOTICS ON IMMUNITY
https://www.youtube.com/watch?v=b_wxaHwJ0aM

Session Plan: 1.2.1h.

Different types of Prebiotics

TYPES OF PREBIOTICS
https://www.youtube.com/watch?v=ZmzQnYZgsLg

Session Plan: 1.2.1i.

Role of Functional Foods in diabetes, gut health, obesity, cancer, neurodegenerative diseases, etc

Role of Functional Food
https://www.youtube.com/results?search_query=PERSONALISED+FOOD

Session Plan: 1.2.2a.

Extraction Techniques
https://www.youtube.com/watch?v=NgsuPTLkPgs
Soxhlet Extraction
https://www.youtube.com/watch?v=mLq35x0g46g
Cold Press Extraction
https://www.youtube.com/watch?v=ONSw77LMSyI
Super Critical Fluid Extraction
https://www.youtube.com/watch?v=uDf5VmoOl34

Session Plan: 1.2.2b.

Isolation and Purification of Phytochemicals
https://www.youtube.com/watch?v=SnbXQTTHGs4
TLC
https://www.youtube.com/watch?v=e3lRt9XdV0s
Paper Chromatography
https://www.youtube.com/watch?v=23W5Z_redfs
Column Chromatography
https://www.youtube.com/watch?v=i8IT_fzNOOY
Gas Chromatography
https://www.youtube.com/watch?v=4Xaa9WdXVTM
HPLC
https://www.youtube.com/watch?v=ZN7euA1fS4Y
Analysis of Aflatoxin
https://www.youtube.com/watch?v=Y6hIBbC-ZKE

Session Plan: 1.2.2c.

Validation of Phytochemicals: Using different microorganisms and mammalian cell lines
https://www.youtube.com/watch?v=EjnQ3peWRek

Session Plan: 1.3.1a.

Introduction to Nutrigenetics

Nutragenetics
https://www.youtube.com/watch?v=1IxWBEK9Jb0

Session Plan: 1.3.1b.

Introduction to Nutrigenomics

NUTRITIONAL GENOMICS
https://www.youtube.com/watch?v=90zr5v-A3B0

Session Plan: 1.3.1c.

Different types of “omics” study – the basic idea

Different types of “omics” study – basic idea
https://youtu.be/eu2BgTBATnQ
References: https://www.labroots.com/trending/cancer/11894/five-omics-fields-introduced

Session Plan: 1.3.1d.

Human Genome Project

Human Genome Project
https://www.youtube.com/watch?v=7PifyYJOvkA

Session Plan: 1.3.1e.

Central dogma – basic concepts

The Central Dogma
https://www.youtube.com/watch?v=gG7uCskUOrA

Session Plan: 1.3.1f.

Concept of Personalized food with special references to athletes

Concept of Personalized food
https://www.youtube.com/watch?v=TH0Jjzw2EqA

Session Plan: 1.3.1g.

Introduction to different types of primer designing

Primer Designing
https://www.youtube.com/watch?v=c-f1H07D_70
https://www.youtube.com/watch?v=tUyBwiyMsSU

Session Plan: 1.3.1h.

Gene expression, upregulation, and downregulation of genes

Gene Expression
https://www.youtube.com/watch?v=Vx15HByfsxc

Session Plan: 1.3.1i.

Genes associated with metabolic disorders

Metabolic Disorder
https://www.youtube.com/watch?v=90zr5v-A3B0

Session Plan: 1.3.1j.

Understanding Food – gene interface (based on recent research publications)

Food gene interphase
https://www.youtube.com/watch?v=wuKQg0cGL0M

Session Plan: 1.3.2a.

m-RNA extraction from treated mammalian cell line
https://www.youtube.com/watch?v=L5XlB8KkKt4

Session Plan: 1.3.2b.

Preparation of c-DNA
https://www.youtube.com/watch?v=iVHxYedAwSA

Session Plan: 1.3.2c.

Study of Gene expression in Real Time PCR
https://www.youtube.com/watch?v=DH7o9Df5_50

Session Plan: 1.3.2d.

Electrophoresis
https://www.jove.com/video/3923/agarose-gel-electrophoresis-for-the-separation-of-dna-fragments

List of Projects/ papers/jobs/products to be done in domain:

2. Projects

2.1. To develop personalized food and beverages rich in Antioxidants and Polyphenols
This project includes the extraction of phytochemicals, identification of bioactive compound(s), isolation of this compound, and incorporation in food/beverages. This food/beverages will be specific for people suffering from hyperglycemia, obesity, or may target athletes to provide instant energy.
2.2. To develop low-cost omega-3 capsules from fish oil/ walnuts
PUFAs like omega-3 fatty acids found in fish oil, walnuts can reduce inflammation and improve hypertriglyceridemia. Formulation of this type of capsule involves drug designing (using Discovery Studio, Biovia) and clinical trials.
2.3. To develop immune boosters
Immune boosters are most suitable for children below 10 years and the aged person above 60 years. This will strengthen the internal immunity of the body and will help to combat the common cold viruses, cases of flu, etc.
2.4. To develop protein bars
Protein bars are popular among the young generation as they are healthy and tasty. Low-calorie protein bars, rich with fruits and nuts may be a popular product with high market value. The level of carbohydrate, fats, proteins, vitamins need to be standardized and hence basic food biochemistry analysis will be involved.
2.5. To develop the extraction of antioxidants and polyphenols from different sources
SCFE (using carbon dioxide) of antioxidants and polyphenols from different sources (eg: Ramie, Dragon Fruit, Berries) can be performed in Gram Tarang Foods Pvt. Ltd. Besides, soxhlet, cold press methods can be explored for finding a commercial way of extraction and supplying it to different food/beverage companies. This project could be beneficial for getting the monetary benefits as well as getting publication by studying the biological efficacy of the plant extracts.
2.6. In-silico Validation of the Phytochemicals on Fish cell line and Plant Microbes
The bioactive molecules from phytochemicals can be used against different types of plant pathogens as well as can be used as fish feed to protect from bacterial or fungal action. This will include molecular docking, pharmacophore modelling. In- silico models will be validated using wet lab tests. Quality publications are expected.
2.7. Drug Designing
Known bioactive molecules from natural resources will be used for drug designing against different human physiological disorders, such as, Hypertension, Hyperglycemia, Parkinson’s disease and certain metabolic disorders. Validation of the drugs warrants clinical trial and may lead to patent and publications.
2.8. In-silico Validation of Nanoparticle dosages on Fish Cell Line and Plant Microbes
Studies on nanoparticles and its applications in plant pathology and fishery sciences are gaining immense popularity. In-silico models will can provide proper experimental design to many research works related to fisheries and plant pathology that would save time, money and man power. Validation of in-silico data in wet lab may lead to quality publications.

3. Products/Patents/Publications

All of the above projects ultimately lead to product development and a decent amount of quality publications. Project (1) and (2) have a high potential to get patents if the proper clinical trials can be conducted.

Course Materials

Session Plan & Materials

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