Faculty: Kajal Samantara

Course Name: Seed Production Using Manual and Molecular Methods

Code(Credit): ABC

 

Course Objectives

High-throughput technologies produce massive amounts of data, much too large to analyze by hand. The objective of this course is:

• To strengthen the student’s knowledge in the subject of the breeding approach to seed production and impart training for entrepreneurship in commercial seed production
• This course will enhance the knowledge of seed biology, seed quality, seed production, seed storage, and seed certification
• Characterization of released and/or improved varieties/hybrids using morphological, biochemical, and molecular markers for varietal identity and registration
• To impart training for the entrepreneurship programme
• To initiate basic research related to genetic purity, seed health, and seed storage

Learning Outcomes

At the end of this course, learners are expected to learn:

• Core competency in the subject & comparative evidence on development of seed and hybrid seed production
• High analytical ability in understanding the application of scientific principles and students will acquire skills & handling operations of different equipment's in seed science laboratory
• Seed production techniques of various important crops will benefit those who would like to venture into seed production companies

SUBJECT I: BREEDING APPROACH TO SEED PRODUCTION
Module: I
Floral Biology, Seed Development and Maturation (10 hr)
Floral types, structure and biology in relation to pollination mechanisms; Sporogenesis: microsporogenesis and megasporogenesis; Gametogenesis - development of male and female gametes and their structures;  Fertilization – embryo sac structure, process, barriers to fertilization, incompatibility and male sterility, factors affecting fertilization; Embryogenesis - development of typical monocot and dicot embryos; Endosperm development, modification of food storage structures with reference to crop plants; Different types of embryos, endosperm and cotyledons; Seed coat structure and development in representative crop plants; Apomixis – identification, classification, significance and its utilization in different crops for hybrid seed production; Causes of embryo abortion, embryo rescue and synthetic seeds.
Module II
Post harvest handling of seeds (10 hr)
Threshing methods; Drying - methods of seed drying; Seed processing - seed cleaning and grading; Processing equipments -cleaner cum grader; Upgrading equipments working principles - specific gravity separator, colour sorter, indented cylinder separator, spiral separator, magnetic separator, needle separator; Seed quality enhancement techniques; Importance - seed fortification, seed priming, seed coating, seed pelleting.
Module III
Seed legislation (10 hr)
Seeds Act 1966; Seed Rules 1968; Seed Control Order, 1983; and Seed Bill 2004; Seed law enforcement; Duty and powers of seed inspector, offences and penalties;  Seeds Control Order 1983; Salient features of PPV&FRA 2001; National Seed Policy 2002; and Seed Bill 2004.
Module IV
Seed Quality Testing (10 hr)
Seed quality assessment - importance; Varietal identification through grow out test, molecular and biochemical test; Detection of genetically modified crops; Field inspection principles and methods- Inspection at harvesting, threshing and processing; Pre-and post-processing quality testing of seed; Seed testing concepts and objectives, its role in seed quality control; Seed sampling, seed moisture testing, purity analysis, germination testing, tolerance limit and seed testing equipments; Quick viability test and seed standards; Seed vigour-its significance and testing methods; Post harvest inspection - processing, bagging and tagging.
Module V
Seed Storage and marketing (10 hr)
Seed storage-principles; Factors affecting seed longevity during storage; Seed treatments and packaging materials; Measures for pest and disease control during storage and godown sanitation; Seed marketing-structure and organization; Sales promotional activities; Factors affecting seed marketing and demand; International seed movement; Role of international organizations, WTO and OECD in seed trade.
Module: VI
Emerging Trends in Seed Quality Enhancement (10 hr)
Concept and significance of seed quality enhancement; Physical, chemical and pesticidal seed treatments, history, principles and methods of seed treatment, methodology and factors affecting seed enhancement treatments; Seed priming: physiological and biochemical basis, types of priming technology, biochemical and molecular changes associated, pregermiantion, film coating and pelleting, seed tapes, seed mats, seed colouring, biopriming;  Synthetic seeds – Aim and scope for synthetic seeds, historical development, somatic embryogenesis, somaclonal variation and their control, embryo encapsulation systems, hardening of artificial seeds, cryopreservation, storage of artificial seeds, desiccation tolerance, use of botanicals in improving seed quality etc.
SUBJECT II: SEED PRODUCTION TECHNOLOGY
Module: I
Principles of Seed Production (10 hr)
Introduction: Seed as basic input in agriculture; seed development in cultivated plants; seed quality concept and importance of genetic purity in seed production; types of cultivars, their maintenance and factors responsible for deterioration; seed production in self and cross pollinated crops. Mode of pollination and reproduction in crop plants and their modification in relation to hybrid seed production. Principles of hybrid seed production, isolation distance, synchronization of flowering, rouging etc. male sterility and incompatibility system in hybrid seed production, role of pollinators and their management; Seed multiplication ratios, seed replacement rate, demand and supply; suitable areas of seed production and storage, agronomy of seed production – agro climatic requirements and their influence on quality seed production; generation system of seed multiplication; maintenance of Nucleus seed, production of Breeder, Foundation and Certified seed– criteria involved; life span of a variety and causes for its deterioration; certification standards for self and cross pollinated and vegetative propagated crops. Planning of seed production for different classes of seeds for self and cross pollinated crops, Seed quality control system and organization, seed village concept; Seed production agencies, seed industry and custom seed production in India.
Module: II
Seed Production in Field Crops (10 hr)
Basic principles in seed production and importance of quality seed. Floral structure, breeding and pollination mechanism in self-pollinated cereals and millets viz, wheat, barley, paddy, ragi etc. Floral structure, breeding and pollination mechanism in cross-pollinated cereals and millets viz maize, sorghum, bajraetc ; methods and techniques of quality seed production incross-pollinated cereals and millets. Floral structure, breeding and pollination mechanism; methods and techniques of seed production in pulses (pigeon pea, chick pea, green garm, black garm, field beans, peas etc.). Floral structure, breeding and pollination mechanism; methods and techniques of seed production in major oil seeds (groundnut, castor, sunflower, safflower, rape and mustard, linseed, sesame etc.). Floral structure, breeding and pollination mechanism; methods and techniques of seed production in commercial fibers (cotton, jute, mestaetc) and vegetatively propagated crops like sugar cane, potato etc.
Module: III
Seed Production in Vegetables (10 hr)
Introduction; modes of propagation in vegetables. Seed morphology and development in vegetable seeds. Floral biology of these plant species; classification of vegetable crops based on pollination and reproduction behavior; steps in quality seed production; identification of suitable areas/locations for seed production of these crops. Classification based on growth cycle and pollination behavior; methods of seed production; comparison between different methods e.g. seed-to-seed vs. root-to-seed method in radish; seed multiplication ratios in vegetables; pollination mechanisms; sex types, ratios and expression and modification of flowering pattern in cucurbits; nursery raising and transplanting stage. Seed production technology of vegetables viz. solanaceous, cucurbitaceous, leguminous, malvaceous, cole crops, leafy vegetables, root, tuber and bulb crops and spices; harvesting/picking stage and seed extraction in fruit vegetables; clonal propagation and multiplication in tuber crops e.g. Potato, sweet potato, colocasia, tapioca; seed-plot technique in potato tuber seed production; hybrid seed production technology of vegetable crops, TPS (true potato seed) and its production technique; hybrids in vegetables; maintenance of parental lines; use of male sterility and self incompatibility in hybrid seed production, environmental factors related to flowering/bolting in vegetable crops.
Module: IV
Seed Production in Flowers, Medicinal, Fruits and Plantation Crops (10 hr)
Introduction: modes of propagation in fruits, flower and plantation crops. Floral biology of these plant species; classification of medicinal and horticultural crops based on pollination and reproduction behavior; steps in quality seed production; identification of suitable areas/locations for seed production of these crops. Flowers and Medicinal Plants; classification based on growth cycle, reproduction and pollination behavior; nursery requirement, planning and management; technology for quality seed production in important flower species i.e. marigolds, petunias, dahlia, roses, gladiolus, tulips, chrysanthemum etc; development of hybrids and their seed production technology flower plants. Seed production technology of annual medicinal plants viz. isabgol, ashawagandha etc. Fruit and Plantation Crops: role of seed in perennial plant species; classification based on reproduction and pollination behavior; polyembryony and its significance; nursery requirement, planning and management; clonal propagation and multiplication in tropical, sub-tropical and temperate fruits and plantation crops; seed orchards; seed collection, extraction and processing.  IPR issues with special reference to floral and plantation crops.
Module: V
Seed Production in Forage, Pasture and Green Manure Crops (10 hr)
Important pasture and forage legume crops in India; seed requirement and production; classification of forage, pastures and green manure crops; pollination behavior. Factors influencing seed production; maintenance of varietal purity, generation systems of seed multiplication self pollinated crops; seed production in apomictic grasses. Methods and techniques of seed production in important grasses, pastures, legumes and green manure crops; apomictic seed. Selection of seed production areas, influence of season, seed rate and spacing, sowing methods, direct seed sowing, transplanting, pelleting, fertilizer and manure requirement, isolation distance, weed control, pollination and seed setting, seed shattering, seed maturity and stage of harvest, seed collection, economics of seed production of important fodder crops. Seed processing, seed treatment, seed storage, seed viability of these crops.
Module: VI
Hybrid Seed Production (10 hr)
Heterosis: definition, expression and estimation of hybrid vigour; utilization of heterosis in agricultural, horticultural and other crop plants for crop improvement. Pre requisites for hybrid seed production; mechanisms and management of pollination in autogamous and allogamous crops; genetic constitution of varieties, hybrids and basic principles in seed production. Techniques of hybrid seed production - emasculation and crossing: use of self-incompatibility, modification of sex; types of male sterility and exploitation in hybrid development and its use in hybrid seed production; development and maintenance of A, B and R lines. Fertility restoration; use of chemical hybridizing agents, problems of non synchrony in flowering of parental lines and methods to overcome; planting ratios and population density in relation to hybrid seed yield; salient features of hybrid seed production of various crops viz., rice, sorghum, bajra, maize, sunflower, cotton and other major vegetables.
SUBJECT III: MOLECULAR APPROACH
Module: I
DNA Technology – I (10 hr)
Restriction enzymes, Modification enzymes, Ligases and nucleases, Use of restriction enzymes, Isoschizomers, Ligation procedure and applications, Homopolymer tailing, Linkers and DNA adapters, Immunodetection, DNA Methylation
Module: I
DNA Technology – II (10 hr)
DNA hybridization and cot curve, DNA Sequencing, Principles of PCR, PCR methodology, Designing PCR primers, Types of PCR – cDNA synthesis and RT-PCR, Multiplex PCR and identification of PCR products, Applications of PCR technology, Transcriptomics, Principles of pyrosequencing
Module: III
Genetic Engineering – I (10 hr)
Manipulation of purified plasmid, Classification of vectors, Artificial vectors, Different types of cloning vectors, Cosmids, Phagemids – M13, Expression vectors, Vectors for genomic library
Module: IV
Genetic Engineering – II (10 hr)
Cloning strategies for sequencing and detection of clones, Cloning strategies for expression and detection of clones, Genomic library, cDNA library, Gene transformation methods (Direct and Indirect), Introduction to transgenic plants, Application of Transgenics – Case studies
Module: V
Molecular Breeding – I (10 hr)
Types of markers, Molecular markers, Classification of molecular markers based on PCR, Classification of molecular markers based on dominance, Characterisitcs and application of molecular markers, Molecular marker with advantages and limitations – RFLP, Molecular marker with RAPD, Molecular marker with SSR, Molecular marke with AFLP, Molecular marker with AFLP.
Module: VI
Molecular Breeding – II (10 hr)
 Marker assisted selection (MAS), Applications in crop improvement, Practical case studies on MAS, QTL Mapping, Marker assisted back crossing (MABC), 16sRNA typing, Gel electrophoresis, Gel scoring and data analysis for phylogenetic relationship, Use of standard MAS procedures.

SUBJECT I: BREEDING APPROACH TO SEED PRODUCTION

Session 1

Floral types and structureFloral Structure1 Floral structure (Video)

Session 2

Floral types and biology in relation to pollination mechanisms2 pollination Mechanisms (Video)Floral Biology

Session 3

Sporogenesis: microsporogenesis and megasporogenesisSporogenesis3 Microsporogenesis (video)

Session 4

Gametogenesis - development of male and female gametes and their structures4 Megagametogenesis4 Megagametogenesis (Video)

Session 5

Male Sterility and Self-incompatabilityMale Sterility and Self Incompatability5 Male sterility (video)

Session 6

Embryogenesis - development of typical monocot and dicot embryos6 Embryo development6 Embryo Development (video)

Session 7

Endosperm DevelopmentEndosperm Development7 Endosperm development (video)

Session 8

Apomixis – identification, classification, significance and its utilization in different crops for hybrid seed production.Apomixis7 Endosperm development (video)

Session 9

Causes of embryo abortionEmbryo Abortion9 embryo rescue (Video)

Session 10

Embryo rescue and synthetic seeds.Synthetic seeds10 Synthetic seeds (Video)

Session 11

Threshing Methods

Threshing methodsVideo-Threshing methods

Session 12

Seed drying and methods of seed drying

Seed drying and methodsVideo-Seed drying and drying methods

Session 13

Air distribution system and types of seed dryers for heated air- drying

Types of seed dryersVideo-Air distribution system and types of seed dryers for heated air-drying

Session 14

Seed cleaning and methods of seed cleaning

Seed cleaning and methodsVideo-Seed cleaning and methods of seed cleaning

Session 15

Seed processing equipment-Cleaner cum grader

Seed processing equipmentVideo-Seed processing equipment- cleaner cum grader

Session 16

Upgrading equipment working principles- specific gravity separator, colour sorter and indented cylinder separator

Upgrading equipment-IVideo-Upgrading equipment working principles- specific gravity separator, colour sorter and indented cylinder separator

Session 17

Upgrading equipment working principles- spiral separator, magnetic separator and needle separator

Upgrading equipment-IIVideo-Upgrading equipment working principles- spiral separator, magnetic separator and disc separator

Session 18

Seed quality enhancement techniques-Seed treatment

Seed quality enhancement technique-IVideo-Seed quality enhancement technique- seed treatment

Session 19

Seed quality enhancement techniques- special treatments(Hardening, fortification, pelleting and priming

Seed quality enhancement technique-IIVideo-Seed quality enchancement-seed invigouration technique

Session 20

Seed treating equipmentsSeed treating equipmentVideo-Seed treatment equipment

Session 21

History of Seed Legislation

Purpose and History of Seed LegislationVideo - History of Seed Legislation

Session 22

Seeds Act 1966

Seed Act 1966Video - Seeds Act 1966

Session 23

Seed Rules

Seed RulesVideo - Seed Rules

Session 24

Seed Control Order

Seed Control Order 1983Video - Seed Control Order

Session 25

Role of Seed Inspectors

Duties and Powers of Seed Inspectors

Video - Duties of Seed Inspectors

Session 26

Seeds Order 1989

Plants, fruits, and seeds order 1989Video - Seed Order

Session 27

Plant Varieties and Farmer's Rights

The Protection of Plant Varieties and Farmer’s Rights

Video - Farmer's Rights

Session 28

Seed Policy 2002

National Seed Policy 2002

Video - Seed Policy 2002

Session 29

Seed Bill 2004

Seed Bill 2004Video - Seed Policy 2002

Session 30

Seed Bill 2019Seed Bill 2019Video - Seed Amendments 2019

Session 31

Seed Quality Assessment
seed quality assesment Video

Session 32

Molecular and biochemical tests and GMO

(molecular and biochemical tests ,GMO

Video

Session 33

Field Inspection principles-Inspection, Thresing ,Harvesting and Processing

FIELD INSPECTION PRINCIPLES AND METHODS - INSPECTION AT THRESHING ,HARVESTING ,PROCESSING

Video

Session 34

Pre and Post processing Quality Testing

pre and post processing quality testing

Video

Session 35

Seed Testing- Objective & its role in Seed technology

seed testing objective and its role in seed quality pptx

Video

Session 36

Seed Sampling

 seed sampling

Video

Session 37

Seed Testing

 seed testing

Video

Session 38

Viability test

quick viability test

Video

Session 39

Post harvest inspection

post harvest inspection

Video

Session 40

Seed Storage

seed storage

Video

Session 41

Seed Longevity and deterioration

 seed longevity

Video

Session 42

Seed Treatment

 seed treatment

Video

Session 43

Pre and Post processing quality testing

 pre and post processing quality testing pptx (1)

Video

Session 44

Pest and Diseases control during  Storage

pest aand disease control during storage

Video

Session 45

Seed marketing and Organisation

seed marketing and organizational structure (
module 5 )

Video

Session 46

Sales promotion activities in seed marketing

sales promotion activities (
module5 )

Vide0

Session 47

Factor affecting seed marketing and demand

 factors affecting seed marketing and demand

 Video

Session 48

International seed Movements

 international seed movements

Video

Session 49

WTO in Seed Marketing

 wto

video

Session 50

Seed Processing and its SignificanceSeed processing Video

Session 51

Seed Quality Enhancement

Seed Quality Enhancement
Video:

Session 52

Seed Treatment
Seed Treatment
Video:

Session 53

Seed Priming
Seed Priming
Video:

Session 54

Seed Coating
Seed Coating
Video:

Session 55

Seed Pelleting

Seed Pelleting

Video:

Session 56

Seed Tapes, Seed Mats, Seed Colouring
Seed Tapes, Seed Mats, Seed Colouring
Video:

Session 57

Somatic Embryogenesis
Somatic Embryogenesis
Video:

Session 58

Artificial/Synthetic seed
Artificial/Synthetic Seed
Video:

Session 59

Artificial/Synthetic Seed Hardening, Cryopreseravtion, Storage of Synthetic Seed
Artificial/Synthetic Seed Hardening, Cryopreservation, Storage of Synthetic Seed
Video:

Session 60

Use of Botanicals in Improving Seed Quality
Use of Botanicals in Improving Seed Quality
Video:

Session 60

Use of Botanicals in Improving Seed Quality
Use of Botanicals in Improving Seed Quality
Video:

Session 61

                              

Practice Session-I

                              

 Emasculation methods                                   

Session 62

Practice Session -II

Male sterility

Session 63

Practice session-IThreshing , drying and cleaning

Session 64

Practice session-IIGermination, treatment and packing

Session 65

Practice Session-I

Biochemical test for genetic purity

Session 66

Practice session IIgrow out test

Session 67

Practice session I

MEDIUM OF SEED STORAGE STRUCTURES

Session 68

Practice session II

precautions on seed storage

Session 69

Objectives of seed quality enhancement, Seed enhancement techniques, Seed treatment, Equipments for seed treatment, Main  groups of chemical formulations used as seed treatment, Techniques of seed priming,  Seed pelleting and pelleting materials, and Seed pelleting process
Practice Session-I

Session 70

Seed Tapes, Seed Mats, Seed discs, Seed carpets, Seed colouring and Name of botanicals used in different crops
Practice Session-II

Session 71

Assignment I

Session 72

Quiz I

Session 73

Internal Test

Session 74

Practice Test I

SUBJECT II: SEED PRODUCTION TECHNOLOGY

Session 75

Introduction: Seed as basic input in agriculture; seed development in cultivated plants; seed quality concept and importance of genetic purity in seed production.Introduction-to-seed-productionVideo-Introduction to seed production

Session 76

Types of cultivars, their maintenance and factors responsible for deterioration; seed production in self and cross pollinated crops.Deterioration-of-varietyVideo-Deterioration of variety

Session 77

Mode of pollination and reproduction in crop plants and their modification in relation to hybrid seed production, Principles of hybrid seed production, isolation distance, synchronization of flowering, rouging etc.Hybrid-seed-production-FinalVideo-Hybrid seed production

Session 78

Male sterility and incompatibility system in hybrid seed production, role of pollinators and their management.Male-sterility-and-self-incompatibilityVideo-Male sterility and self incompatibility

Session 79

Seed multiplication ratios, seed replacement rate, demand and supply; suitable areas of seed production and storage.Seed-multiplication-ratios-seed-replacement-rate-demand-and-supply-suitable-areas-of-seed-production-and-storageVideo-Seed multiplication ratios seed replacement rate demand and supply suitable areas of seed production and storage

Session 80

Agronomy of seed production – agro climatic requirements and their influence on quality seed production
Agronomy-of-seed-production-and-agroclimatic-zonesVideo-Agronomy of seed production and agroclimatic zones

Session 81

Generation system of seed multiplication; maintenance of Nucleus seed, production of Breeder, Foundation and Certified seed– criteria involvedGeneration-system-of-seed-multiplicationVideo-Generation system of seed multiplication

Session 82

Life span of a variety and causes for its deterioration; certification standards for self and cross pollinated and vegetative propagated cropsLife-span-of-a-variety-and-causes-for-its-deterioration-certification-standards-for-self-and-cross-pollinated-and-vegetative-propagated-cropsVideo-Life span of a variety and causes for its deterioration_ certification standards for self and cross pollinated and vegetative propagated crops.

Session 83

Planning of seed production for different classes of seeds for self and cross pollinated cropsPlanning-of-seed-production-for-different-classes-of-seeds-for-self-and-cross-pollinated-cropsVideo-Planning of seed production for different classes of seeds for self and cross pollinated crops

Session 84

Seed quality control system and organization, seed village concept; Seed production agencies, seed industry and custom seed production in India.Seed-quality-control-system-and-organization-seed-village-concept-Seed-production-agencies-seed-industry-and-custom-seed-production-in-IndiaVideo-Seed quality control system and organization, seed village concept; Seed production agencies, seed industry and custom seed production in India

session- 85

Basic principles in seed production. Seed quality and its importance in seed productionBasic principles of seed production
Seed Qualityvideo- seed quality

session- 86

Floral structure, breeding and pollination mechanism in self-pollinated cereals
floral biology and seed production RICEvideo- floral biology and seed production RICE

session- 87

Floral structure, breeding and pollination mechanism in self-pollinated cerealsfloral biology of barley
flower biology of wheatvideo- floral biology of wheat

Session-88

Floral structure, breeding and pollination mechanism in cross-pollinated cerealsFLORAL BIOLOGY OF MAIZE
FLORAL STRUCTURE AND BREEDING MECHANISM IN MAIZEvideo- FLORAL STRUCTURE AND BREEDING MECHANISM IN MAIZE

Session-89

Floral structure, breeding and pollination mechanism in cross-pollinated cerealsfloral bio of sorghum
floral biology of pearl milletvideo- floral biology of pearl millet

Session-90

Floral structure, breeding and pollination mechanism; methods and techniques of seed production in pulsesfloral biology of blackgram
FLORAL BIOLOGY COWPEA
video- FLORAL BIOLOGY COWPEA

session-91

Floral structure, breeding and pollination mechanism; methods and techniques of seed production in pulsesfloral biology of chickpea
floral biology of garden peavideo- floral biology of chickpea

Session-92

Floral structure, breeding and pollination mechanism; methods and techniques of seed production in major oil seedsFLORAL BIOLOGY OF CASTOR
floral biology of soybeanvideo- FLORAL BIOLOGY OF CASTOR

session-93

Floral structure, breeding and pollination mechanism; methods and techniques of seed production in major oil seedsfloral biology of mustard
SUNFLOWER FLORAL BIOLOGYvideo- floral biology of mustard

session-94

Floral structure, breeding and pollination mechanism; methods and techniques of seed production in commercial fibersFLORAL BIOLOGY OF COTTON
floral biology of jutevideo- FLORAL BIOLOGY OF COTTON

Session 95

Floral Biology & seed development and morphology and Modes of propagation in vegetables
Floral Biology & seed development and morphology and Modes of propagation in vegetables
Video

Session 96

Classification of vegetable cropsClassification of vegetable crops
Video

Session 97

Steps in quality seed production

Steps in quality seed production

Video

Session 98

Hybrid seed production technology of vegetable crops

Hybrid seed production technology of vegetable crops

Video

session- 99

Clonal propagation and Seed plot techniqueClonal propagation and Seed plot technique
Video

session- 100

Seed production technology in BrinjalSeed production technology in Brinjal
Video

session- 101

Seed Production in Bhendi & OnionSeed Production in Bhendi & Onion
Video

session- 102

Seed production technology of ChilliSeed production technology of Chilli
Video

session- 103

Seed production technology of cucurbitaceousSeed production technology of cucurbitaceous
Video

session- 104

Environmental factors related to flowering/bolting in vegetable cropsEnvironmental factors related to flowering bolting in vegetable crops
Video

session- 105

Introduction: modes of propagation in fruits, flower and plantation cropsintroduction to methods of propagationVedio-Introduction to methods of propagationintroduction to methods of propagation

session-106

classification of medicinal and horticultural crops based on pollination and reproduction behaviourPLANT-PROPAGATION
modes of reproductionVedio- Modes of reproductionreproduction

session-107

Flowers: classification based on growth cycle, reproduction and pollination behaviour; nursery requirement, planning and management. Technology for quality seed production in important flower species.Chrysanthemum
MARIGOLDVedio-  Technology for quality seed production in important flower crop chrysanthemum

session-108

Flowers: classification based on growth cycle, reproduction and pollination behaviour; nursery requirement, planning and management. Technology for quality seed production in important flower species.BREEDING IN LILIUM
Carnation
GladiolusVedio- Technology for quality seed production in important flower crop carnation

session-109

Seed production technology of annual medicinal plantsSENNA PPT
isabgol
cinchona
Vedio-Seed production technology of annual medicinal plants Senna

session-110

Fruit Crops: role of seed in perennial plant species; classification based on reproduction and pollination behaviourapple
Mango
papayaVedio-Classification based on reproduction and pollination behaviour in Papaya

session-111

Fruit  Crops: role of seed in perennial plant species; classification based on reproduction and pollination behaviourgrapes
PEACH PPT
pear ppt
plumVedio-Classification based on reproduction and pollination behaviour Peach

session-112

Plantation Crops: role of seed in perennial plant species; classification based on reproduction and pollination behaviourcocoa
coconut ppt
RUBBER PPTVedio-Plantation Crops role of seed in perennial plant species classification based on reproduction and pollination behaviour Rubber

session-113

Polyembryony and its significance; nursery requirement, planning and management.polyembryonyVedio-Polyembryony and its significance

session-114

Clonal propagation and multiplication in tropical, sub-tropical and temperate fruits and plantation cropsclonal propagation
tissue cultureVedio-Clonal propagation and multiplication in tropical, sub-tropical and temperate fruits and plantation crops

Session 115

Important pasture and forage legume crops in India, seed requirement and production
Important pasture and forage legume crops in India, seed requirement and production
video:

Session 116

Classification of forage, pastures and green manure crops; pollination behavior
Classification of forage, pastures and green manure crops; pollination behavior
Video:

Session 117

Factors influencing seed production; maintenance of varietal purity, generation systems of seed multiplication
Factors influencing seed production; maintenance of varietal purity, generation systems of seed multiplication
Video:

Session 118

Seed Production in Apomictic Grasses
Seed Production in Apomictic Grasses
Video:

Session 119

Berseem/Egyptian clover Seed Production
Berseem/Egyptian clover Seed Production
Video:

Session 120

Alfalfa/Lucerne Seed Production
Alfalfa/Lucerne Seed Production
Video:

Session 121

Senji (Indian Clover) Seed Production
Senji (Indian Clover) Seed Production
Video:

Session 122

Seed Processing
Seed Processing
Video:

Session 123

Seed Treatment
Seed Treatment
Video:

Session 124

Seed Storage and Seed Viability
Seed Storage and Seed Viability
Video:

Session 125

Heterosis: definition, expression and estimation of hybrid vigourHeterosisVideo- Heterosis

Session 126

Utilization of heterosis in agricultural, horticultural and other crop plants for crop improvement
Utilization of heterosis in Agriculture and Horticultural cropsVideo- Utilization of heterosis in Agriculture and Horticultural crops

Session 127

Pre requisites for hybrid seed production; mechanisms and management of pollination in autogamous and allogamous crops
Pre requisites for hybrid seed productionVideo- Pre requisites for hybrid seed production

Session 128

Techniques of hybrid seed production - emasculation and crossing, Planting ratios and population density in relation to hybrid seed yield, problems of non-synchrony in flowering of parental linesTechniques in Hybrid Seed Production (Emasculation and Crossing)Video- Techniques in Hybrid Seed Production (Emasculation and Crossing)

Session 129

Self-incompatibility, its type and use in hybrid seed productionSelf Incompatibility and its useVideo- Self Incompatibility and its use

Session 130

Male sterility, its type, and exploitation in hybrid seed development, Fertility restoration; use of chemical hybridizing agentsMale sterility and its use in hybrid seed productionVideo- Male sterility and its use in hybrid seed production

Session 131

Development and maintenance of A, B and R linesDevelopment and maintance of A, B and R lineVideo- Development and maintance of A, B and R line

Session 132

Seed Production in CerealsHybrid seed production of CerealsVideo- Hybrid Seed production of Cereals 

Session 133

Seed Production in Sunflower and CottonSeed production in sunflower and cottonVideo- Seed production in sunflower and cotton

Session 134

Seed Production in Vegetables
Seed Production In VegetablesVideo- Seed Production In Vegetables

Session 135

Seed QualitySeed quality- practice session I

Session 136

Floral Biology of Castorfloral biology of castor- practice session II

Session 137

Floral Biology & seed development and morphology and Modes of propagation in vegetables
Practice Session-I

Session 138

Hybrid seed production technology of vegetable crops
Practice Session-II

Session 139

Practice I classification based on reproduction and pollination behaviour in Papaya
Practice I

Session 140

Practice II Introduction to methods of propagation
Practie II

Session 141

Importance of pasture and forage legume crops, Classification of forage, pastures and green manure crops, Production and Maintenance of Apomictic Hybrid, Flowers and Pollination Dynamics of Egyptian clover, and Seed Standard of Egyptian clover, Lucerne and Senji.
Practice Session-I

Session 142

Seed Processing Plant Layout Planning, Requirements in seed processing, Movement of seed in a processing plant, Seed processing equipments, Types of storage, Classification of packing materials or containers, and Maintenance of viability in storage.
Practice Session-II

Session 143

Types of Heterosis and Prediction of Performance of double ross hybrid
Practice session 1 Types of Heterosis and prediction of Performance of double cross hybrids

Session 144

Different Methods of Emasculation Techniques
Practice session 2 Different Methods of Emasculation Techniques

Session 145

Quiz II

Session 146

Quiz III

Session 147

Assignment III

Session 148

Practice Test II

Session 149

SUBJECT III- MOLECULAR APPROACH

Session 150

DNA technology-I 

Module 1

Restriction enzymes-Classification,nomenclatureRestriction Enzymesvideo: Restriction Enzymes

Session 151

Modification enzymesMODIFICATION ENZYMESvideo: Modification Enzymes

Session 152

Ligases and Nucleases
Ligases and Nucleasesvideo: Ligases and Nucleases

Session 153

Uses of restriction enzymes
Use of Restriction Enzymesvideo: Use of Restriction Enzymes

Session 154

IsochizomersIsochizomersvideo: Isochizomers

Session 155

Ligation procedure and applications
Ligation Procedure and Applicationvideo: Ligation procedure and application

Session 156

Homopolymer tailingHomopolymer Tailingvideo: Homopolymer tailing

Session 157

Linkers and DNA adapters
Linkers and DNA Adaptersvideo: Linkers and DNA adapters

Session 158

ImmunodetectionImmunodetectionvideo: Immunodetection

Session 159

DNA methylationDNA Methylationvideo: DNA Methylation

Module 2

Session 160

DNA hybridization and Cot CurveDNA hybridization and Cot curvevideo: DNA hybridisation and cot curve

Session 161

DNA sequencing (Sanger Method)DNA seq sanger methodvideo: DNA sanger sequencing method

Session 162

Polymerase Chain Reaction (PCR)-Principles of PCRPCR principlevideo: PCR principle

Session 163

PCR MethodologyPCR-Methodologyvideo: PCR methodology

Session 164

Designing PCR PrimersPCR_Primer_Designvideo: primer design

Session 165

Types of PCR, CDNA Synthesis and RT-PCRTypes of PCR, cDNA Synthesis and RT-PCRVideo: Types of PCR, cDNA Synthesis and RT-PCR

Session 166

Multiplex PCR and Identification of PCR Products
Multiplex PCRVideo: Multiplex PCR

Session 167

Applications of PCR technologyApplications of PCRVideo: Application of PCR

Session 168

Transcriptomics: Definition and Applications of Transcriptomics and NGS in briefTranscriptomicsVideo: Transcriptomics

Session 169

Principles of PyrosequencingPyrosequencingVideo: Pyrosequencing

Session 170

Manipulation of purified plasmidManipulation of purified plasmidVideo-Plasmid purification & Manipulation

Session 171

Classification of vectorsClassification of VectorsVideo-Classification of Vectors

Session 172

Artificial vectorsArtificial vectors-Construction of prototype vector (pBR322)Video-Artificial Vectors_ Construction of pBR322

Session 173

Different types of cloning vectors, Plasmid-pUC 19, λ Phage.Different types of cloning vectors, Plasmid-pUC 19, λ Phage.Video-Cloning vectors- Plasmid-pUC 19, λ Phage.

Session 174

CosmidsCosmidsVideo-Cosmids

Session 175

PhagemidsPhagemids-M13Video-Phagemid_and_M13_vector

Session 176

Types of expression vectorsTypes of Expression VectorsVideo-Types_of_Expression_Vectors

Session 177

Vectors for genomic library in genetic engineering-Bacterial Artificial ChromosomesVectors for Genomic library in genetic engineering-Bacterial Artificial ChromosomesVideo-Bacterial_Artificial_Chromosomes

Session 178

Vectors for genomic library in genetic engineering-Yeast Artificial ChromosomesVectors for Genomic library in genetic engineering-Yeast Artificial ChromosomesVideo-Yeast_Artificial_Chromosomes

Session 179

Vectors for genomic library in genetic engineering-P1-derived Artificial ChromosomesVectors for Genomic library in genetic engineering-P1-derived Artificial ChromosomesVideo-P1_derived_artificial_chromosome

SUBJECT III: MOLECULAR APPROACH

Session 180

Cloning strategies for Sequencing and Detection of clonesGene CloningVideo: Basics of protein structure

Session 181

DNA delivery systemsVectorsVideo: Vectors

Session 182

Genomic Library- strategies, construction and applicationGenetic librariesVideo: Genetic libraries

Session 183

cDNA Library-- strategies, construction and applicationcDNA LibraryVideo: cDNA library

Session 184

Gene transformation methodsTransformationBacterial transformantsVideo: Transformation

Session 185

Gene transformation methods (Indirect)Indirect gene transfer methodshttps://courseware.cutm.ac.in/files/2021/02/Gene-transfer-in-plants.mp4

Session 186

Introduction to transgenic plantsTransgenic plantshttps://courseware.cutm.ac.in/files/2021/02/Transgenic-Plants.MP4.mp4

Session 187

Application of Transgenics- case study-ITransgenic plants (Case study-I)https://courseware.cutm.ac.in/files/2021/02/Application-of-transgenic-plants-Case-study-I.mp4

Session 188

Application of Transgenics- case study-IITransgenic plants (Case study-II)https://courseware.cutm.ac.in/files/2021/02/Transgenic-plants-case-study-II.MP4.mp4

Session 189

Application of Transgenics- case study-IIITransgenic plants (Case study-III)https://courseware.cutm.ac.in/files/2021/02/Traqnsgenic-rice-case-study-III.mp4

Session 190

Molecular Markers-Introduction190: Introduction to Molecular Marker190: https://courseware.cutm.ac.in/files/2021/02/190-Introduction-to-MolecularGenetic-Markers.mp4

Session 191

Types of Molecular markers: Hybridization based191 Hybridization based Markers GPL191: https://courseware.cutm.ac.in/files/2021/02/191-Hybridization-and-PCR-based-Markers-GPL.mp4

Session 192

Types of Molecular Markers192 Types of Molecular Markers and it's applications GPL192: https://courseware.cutm.ac.in/files/2021/02/192-Markers-Types-and-Applications-193.mp4

Session 193

Types of Molecular Markers and it's applications193 Types of Molecular Markers and it's applications GPL193: https://courseware.cutm.ac.in/files/2021/02/192-Markers-Types-and-Applications-193.mp4

Session 194

Sequence based molecular markers: SNPs194 Sequence based molecular markers - SNPs GPL194: https://courseware.cutm.ac.in/files/2021/02/194-Sequence-based-molecular-markers-SNPs.mp4

Session 195

Molecular Marker with advantages and limitations - RFLPs195 Molecular Marker with advantages and limitations - RFLPs GPL195: https://courseware.cutm.ac.in/files/2021/02/195-Molecular-Marker-with-advantages-and-limitations-RFLPs.mp4

Session 196

Molecular Marker with advantages and limitations -RAPD196 Molecular Marker with advantages and limitations -RAPD GPL196: https://courseware.cutm.ac.in/files/2021/02/196-Molecular-Marker-with-advantages-and-limitations-RAPD.mp4

Session 197

Molecular Marker with advantages and limitations -SSR197 Sequence based molecular markers SSRs GPL197: https://courseware.cutm.ac.in/files/2021/02/197-Molecular-Marker-with-advantages-and-limitations-SSR.mp4

Session 198

Molecular Marker with advantages and limitations –AFLP198 Molecular Marker with advantages and limitations –AFLP GPL198: https://courseware.cutm.ac.in/files/2021/02/198-Molecular-Marker-with-advantages-and-limitations-–AFLP.mp4

Session 199

Molecular Markers Case Study:199 Molecular Markers Case Study GPL199: https://courseware.cutm.ac.in/files/2021/02/199-Genetic-Markers-Case-Study.mp4

Session 200

Marker assisted selection (MAS) and MABCMarker Assisted SelectionVideo: MAS

Session 201

Marker assisted backcross breeding (MABC): Applications in crop improvement-case studiesMABCVideo: MABC

Session 202

QTL mapping: Definition and case studiesQTL-case study
QTL case study

Session 203

Genome-wide Association Mapping-GWASGWAShttps://courseware.cutm.ac.in/files/2021/02/GWAS-2.pptx

Session 204

Case studies-GWASCase studies-GWAS

Session 205

Use of standard MAS procedures- screening crops for resistance to different biotic stress traits.
MAS-biotic stress resistance in crops
MAS in biotic stress resistance video

Session 206

Use of standard MAS procedures- screening crops for resistance to different abiotic stress traits.
MAS-abiotic resistance in crops
MAS in abiotic stress resistance-Video

Session 207

Mutation Breeding
Mutation breeding
Mutation Breeding vdo

Session 208

Advanced breeding tools for seed productionAdvanced breeding tools for seed production
Video: Advanced breeding tools

Session 209

RAD-SeqRestriction site-associated DNA sequencingVideo: RAD-Seq

Session 210

Isolation of Plant genome DNA from F1 progenies and its visualization on Agarose GEl ElectrophoresisIsolation of plant genomic DNA from its F1 progenies and visualization by gel electrophoresis

Session 211

Isolation of RNA and its quantificationIsolation of RNA and its quantification

Session 212

Restriction DigestionRestriction Digestion

Session 213

Quantative determination of proteins by UV, visible SpectrophotometerDetermination of Protein by UV Spectroscopy

Session 214

Characterization of Proteins using SDS-Polyacrylamide gel electrophoresis (Silver staining of proteins)Characterization of Proteins using SDS-Polyacrylamide gel electrophoresis (Silver staining of proteins)Video-SDS_PAGE

Session 215

Blotting Techniques for Protein - Western blottingSDS PAGEVideo: Western blotting

Session 216

Gene amplification by Polymerase Chain Reaction (PCR) and qRTPCRhttps://www.promega.in/resources/guides/nucleic-acid-analysis/pcr-amplification/PCR quick-protocol;
PCR-protocol;
qRT-PCR-system-protocol

Session 217

Practice 28: Check the hybrid (F1) fidelity using molecular markers217 Check the hybrid (F1) fidelity using molecular markers GPL217: https://courseware.cutm.ac.in/files/2021/02/217-Hybrid-Fidelity-using-Molecular-Markers.mp4

Session 218

Molecular Tests for seed quality assessment.
seed quality acessment through molecular methods
Seed Quality assessment_vdo

Session 219

Practice 30: Biochemical test for seed quality assessmentBiochemical tests

Session 220

Assignment IV

Session 221

Quiz IV

Session 222

Final Exam

Session 223

Practice Test III

Course Materials

• fpls-11-00359
• Gene Cloning
• Vectors
• Transformation
• Bacterial transformants
• Genetic libraries
• cDNA Library
• Basics of protein structure
• session 4.1 -seed quality assesment ( session 1)
• session 4.2 - (molecular and biochemical tests ,GMO (
  MODULE -4 )
• session 4.3 - (FIELD INSPECTION PRINCIPLES AND METHODS - INSPECTION AT THRESHING ,HARVESTING ,PROCESSING
• session 4.4 - pre and post processing quality testing pptx (1)
• session 4.5 -seed testing objective and its role in seed quality pptx (1)
• session 4.6 seed samplig
• session 4.7 seed testing
• session 4.8 quick viability test
• session 4.9 - seed vigour and testing methods
• session 4.9 - seed vigour and testing methods
• session 4.10 post harvest inspection. pptx (1)
• session 5.1 -seed storage (
  module 5 )
• session 5.2 seed longevity
• sesssion 5.3 seed treatment
• session 4.4 - pre and post processing quality testing pptx (1)
• session 5.5 -pest aand disease control during storage
• sesssion 5.6 -seed marketing and organizational structure (
  module 5 )
• session 5.7 -sales promotion activities (
  module5 )
• session 5.8 factors affecting seed marketing and demand pptx
• session 5.9 international seed movements
• session 5.10 wto
• session 4.1 -seed quality assesment ( session 1)
• 1_Introduction to seed production
• 2_Deterioration of variety
• 3_Hybrid seed production Final
• 4_Male sterility and self incompatibility
• 5_Seed multiplication ratios, seed replacement rate, demand and supply; suitable areas of seed production and storage
• 1_Introduction to seed production
• 2_Deterioration of variety
• 3_Hybrid seed production Final
• 4_Male sterility and self incompatibility
• Indirect gene transfer methods
• Transgenic plants
• Transgenic plants (Case study-I)
• Transgenic plants (Case study-II)
• Transgenic plants (Case study-III)
• GWAS
• GWAS-case study
• QTL-case study
• Session 1
• Session 11
• Session 12
• Session 13
• Session 13
• Session 14
• Session 15
• Session 16
• Session 18
• Session 17
• Session 18
• Session 19
• Session 20
• Session 1 - Purpose and History of Seed Legislation
• Session 2 -Seed Act 1966
• Session 3 - Seed Rules
• Session 4 - Seed Control Order 1983
• Session 5 -Duties and Powers of Seed Inspectors
• Session 6-Plants, fruits, and seeds order 1989
• Session 7 - The Protection of Plant Varieties and Farmer’s Rights
• Session 8 - National Seed Policy 2002
• Session 9 - Seed Bill 2004
• MAS 2.0
• Marker Assisted Selection
• Advanced breeding tools for seed production
• Session 1-Heterosis
• Session 2- Utilization of heterosis in Agriculture and Horticultural crops
• Session 3-Pre requisites for hybrid seed production
• Session 4-Techniques in Hybrid Seed Production (Emasculation and Crossing)
• Session 5-Self Incompatibility and its use
• Session 6-Male sterility and its use in hybrid seed production
• Session 7-Development and maintance of A, B and R line
• Session 8-Hybrid seed production of Cereals
• Session 9-Seed production in sunflower and cotton
• Session 10- Seed Production In Vegetables
• MODIFICATION ENZYMES
• Ligases and Nucleases
• Session 8 - National Seed Policy 2002
• Session 11
• Homopolymer Tailing
• Linkers and DNA Adapters
• Immunodection
• 1. Threshing methods
• 2. Seed drying and methods
• Immunodetection
• 4. Seed cleaning and methods
• 3. Types of seed dryers
• DNA Methylation
• 4. Seed cleaning and methods
• 5. Seed processing equipment
• 6. Upgrading equipment-I
• 7. Upgrading equipment-II
• 8. Seed quality enhancement technique-I
• 9. Seed quality enhancement technique-II
• 10. Seed treating equipment
• MABC
• SDS PAGE
• Use of Restriction Enzymes
• Isochizomers
• Ligation Procedure and Application
• Floral Structure
• Session 2
• 
  Module 1 Session 3
• 
  Module 1 Session 4
• 
  Module 1 Session 5
• 
  Module 1 session 6
• 
  Module 1 Session 7
• 
  Module 1 Session 8
• 
  Module 1 Session 9
• 
  Module 1 Session 10
• kjpic
• Session 170_ Manipulation of purified plasmid
• Manipulation of purified plasmid
• Classification of Vectors
• Artificial vectors-Construction of prototype vector (pBR322)
• Different types of cloning vectors, Plasmid-pUC 19, λ Phage.
• Phagemids-M13
• Cosmids
• Phagemids-M13
• 6. Agronomy of seed production and agroclimatic zones-converted
• DNA hybridization and Cot curve
• DNA seq sanger method
• PCR principle
• PCR-Methodology
• PCR_Primer_Design
• Determination of Protein by UV Spectroscopy
• 7_Generation system of seed multiplication-converted
• 7_Generation system of seed multiplication
• 8_Life span of a variety and causes for its deterioration; certification standards for self and cross pollinated and vegetative propagated crops.
• 9_Planning of seed production for different classes of seeds for self and cross pollinated crops
• 10_Seed quality control system and organization, seed village concept; Seed production agencies, seed industry and custom seed production in India
• Basic principles of seed production
• Seed Quality
• Modes of propagation in vegetables, Seed morphology
• Modes of propagation in vegetables, Seed morphology
• Floral Biology & seed development and morphology and Modes of propagation in vegetables
• Classification of vegetable crops
• Steps in quality seed production
• Hybrid seed production technology of vegetable crops
• Clonal propagation and Seed plot technique
• Clonal propagation and Seed plot technique
• Clonal propagation and Seed plot technique
• Clonal propagation and Seed plot technique
• Seed production technology in Brinjal
• Seed Production in Bhendi & Onion
• Seed production technology of Chili
• Seed production technology of cucurbitaceous
• floral biology and seed production RICE
• flower biology of wheat
• floral biology of barley
• Seed production technology of cucurbitaceous
• floral bio of sorghum
• FLORAL BIOLOGY OF MAIZE
• FLORAL STRUCTURE AND BREEDING MECHANISM IN MAIZE
• Environmental factors related to floweringbolting in vegetable crops
• Seed Quality
• flower biology of wheat
• floral bio of sorghum
• floral biology of pearl millet
• floral biology of blackgram
• FLORAL BIOLOGY COWPEA
• floral biology of chickpea
• floral biology of garden pea
• FLORAL BIOLOGY OF CASTOR
• floral biology of soybean
• FLORAL BIOLOGY OF CASTOR
• floral biology of soybean
• Session 179_Vectors for Genomic library in genetic engineering-P1-derived Artificial Chromosomes
• Session 178_Vectors for Genomic library in genetic engineering-Yeast Artificial Chromosomes
• Session 177_Vectors for Genomic library in genetic engineering-Bacterial Artificial Chromosomes
• Session 176_Types of Expression Vectors
• Biochemical Tests
• RAD_Sequencing
• Session 10 - Seed Bill 2019
• floral biology of musturd
• SUNFLOWER FLORAL BIOLOGY
• FLORAL BIOLOGY OF COTTON
• floral biology of jute
• Molecular markers GPL
• Hybridization Based Markers
• Molecular Marker Types
• DNA molecular markers in plant breeding current status and recent advancements in genomic selection and genome editing
• Sequence based molecular markers - SNPs
• DNA molecular markers, types and applications
• Molecular Marker with advantages and limitations - RFLPs
• PCR amplification assays-RAPD
• Molecular Marker - RAPD
• Session 10 - Seed Bill 2019
• introduction to methods of propagation
• PLANT-PROPAGATION
• Chrysanthemum
• MARIGOLD
• SENNA PPT
• apple
• Mango
• papayalecture-180307042621 (2)
• apple
• Mango
• papaya
• cocoa
• coconut ppt
• RUBBER PPT
• BREEDING IN LILIUM
• Carnation
• Gladiolus
• grapes
• PEACH PPT
• pear ppt - Copy
• plum
• polyembryony
• clonal propagation
• tissue culture
• modes of reproduction
• cinchona
• isabgol
• 1. Threshing methods
• 2. Seed drying and methods
• 4. Seed cleaning and methods
• 3. Types of seed dryers
• 4. Seed cleaning and methods
• 5. Seed processing equipment
• 6. Upgrading equipment-I
• 7. Upgrading equipment-II
• 8. Seed quality enhancement technique-I
• 9. Seed quality enhancement technique-II
• 10. Seed treating equipment
• 75_Introduction-to-seed-production
• 76_Deterioration-of-variety
• 77_Hybrid-seed-production-Final
• 78_Male-sterility-and-self-incompatibility
• 79_Seed-multiplication-ratios-seed-replacement-rate-demand-and-supply-suitable-areas-of-seed-production-and-storage
• Threshing methods
• Seed drying and methods
• 80_Agronomy-of-seed-production-and-agroclimatic-zones
• Types of seed dryers
• 81_Generation-system-of-seed-multiplication
• Seed cleaning and methods
• Seed processing equipment
• Upgrading equipment-I
• Upgrading equipment-II
• Seed quality enhancement technique-I
• Seed quality enhancement technique-II
• Seed treating equipment
• 82_Life-span-of-a-variety-and-causes-for-its-deterioration-certification-standards-for-self-and-cross-pollinated-and-vegetative-propagated-crops
• 83_Planning-of-seed-production-for-different-classes-of-seeds-for-self-and-cross-pollinated-crops
• 84_Seed-quality-control-system-and-organization-seed-village-concept-Seed-production-agencies-seed-industry-and-custom-seed-production-in-India
• Session 10 - Seed Bill 2019
• MAS-biotic stress resistance in crops
• Mutation breeding
• seed quality acessment through molecular methods
• MAS-abiotic resistance in crops
• Introduction to microsatellites
• Molecular Marker with advantages and limitations Notes – AFLP
• Floral Biology
• Sporogenesis
• Gametogenesis
• Male Sterility and Self Incompatability
• Embryogenesis
• Endosperm Development
• Apomixis
• Embryo Abortion
• Synthetic seeds
• CASEStudy Molecular Markers
• Molecular Markers for Genetic Fidelity
• Biochemical tests
• Session 176_Types of Expression Vectors
• Session 177_Vectors for Genomic library in genetic engineering-Bacterial Artificial Chromosomes
• Session 178_Vectors for Genomic library in genetic engineering-Yeast Artificial Chromosomes
• Session 179_Vectors for Genomic library in genetic engineering-P1-derived Artificial Chromosomes
• Restriction Digestion
• Session 214_ Characterization of Proteins using SDS-Polyacrylamide gel electrophoresis (Silver staining of proteins)
• Restriction site-associated DNA sequencing-converted
• Restriction site-associated DNA sequencing
• Threshing methods
• Seed drying and methods
• Types of seed dryers
• Seed cleaning and methods
• Seed processing equipment
• Upgrading equipment-I
• Upgrading equipment-II
• Seed quality enhancement technique-I
• Seed quality enhancement technique-II
• Seed treating equipment
• Isolation of RNA and its application
• Isolation of RNA and its quantification
• Threshing methods
• Seed drying and methods
• Types of seed dryers
• Seed cleaning and methods
• Seed processing equipment
• Upgrading equipment-I
• Upgrading equipment-II
• Seed quality enhancement technique-I
• Seed quality enhancement technique-II
• Seed quality enhancement technique-II
• Seed treating equipment
• Seed processing
• Types of PCR, cDNA Synthesis and RT-PCR
• Types of PCR, cDNA Synthesis and RT-PCR
• Multiplex PCR
• Applications of PCR
• Transcriptomics
• Pyrosequencing
• PCR quick-protocol
• PCR-protocol
• qRT-PCR-system-protocol
• Practice on PCR and qRT-PCR
• Seed quality enhancement 51
• Seed Quality Enhancement 51
• health education and community optometry
• Seed-quality-enhancement-51-2.pdf
• Use of Botanical for Seed Quality
• Seed Quality Enhancement
• Seed Priming
• Seed Coating
• Seed Pelleting
• Seed Tapes, Seed Mats, Seed Colouring
• Somatic Embryogenesis
• Artificial/Synthetic Seed
• Artificial/Synthetic Seed Hardening, Cryopreservation, Storage of Synthetic Seed
• Use of Botanicals in Improving Seed Quality
• Important pasture and forage legume crops in India, seed requirement and production
• Classification of forage, pastures and green manure crops; pollination behavior
• Factors influencing seed production; maintenance of varietal purity, generation systems of seed multiplication
• Seed Production in Apomictic Grasses
• Berseem/Egyptian clover Seed Production
• Alfalfa/Lucerne Seed Production
• Senji (Indian Clover) Seed Production
• Seed Processing
• Seed Treatment
• Seed Storage and Seed Viability
• Session 1
• Floral Biology & seed development and morphology and Modes of propagation in vegetables
• Clonal propagation and Seed plot technique
• Seed production technology in Brinjal
• Seed Production in Bhendi & Onion
• Seed production technology of Chili
• Seed production technology of cucurbitaceous
• Environmental factors related to floweringbolting in vegetable crops
• Session 1
• Isolation of plant genomic DNA from its F1 progenies and visualization by gel electrophoresis
• Vectors
• 1
• 2
• 3
• 4
• 5
• 6
• cDNA Library
• 7
• 8
• 9
• 10
• Genetic libraries
• cDNA Library
• Heterosis
• Utilization of heterosis in Agriculture and Horticultural crops
• Techniques in Hybrid Seed Production (Emasculation and Crossing)
• Development and maintance of A, B and R line
• HYBRID SEED PRODUCTION OF RICE, SORGHUM, BAJRA
• Seed production in sunflower and cotton
• Session 1
• Session 2
• Restriction_Enzymes
• 1
• 2
• 2
• 2
• Important Pasture and Forage crop in India
• 3
• 4
• 5
• 6
• 7
• 8
• 9
• 10
• Seed quality enhancement-51
• Seed treatment-52
• Use of botanical in seed quality-60
• Hardening of Synthetic seed-59
• Synthetic seed-58
• Somatic SE-57
• Seed tape, seed mat and seed colouring-6
• Seed pelleting-55
• Seedcoating-54
• Genetic libraries
• cDNA Library
• Transformation
• Session 1 - Purpose and History of Seed Legislation - Narration
• Session 1 - Purpose and History of Seed Legislation - Narration
• MEDIUM OF SEED STORAGE STRUCTURES
• Biochemical test for genetic purity
• grow out test
• precautions on seed storage
• MEDIUM OF SEED STORAGE STRUCTURES
• MEDIUM OF SEED STORAGE STRUCTURES
• precautions on seed storage
• introduction to methods of propagation
• modes of reproduction
• chrysanthemum
• carnation
• senna
• papaya
• peach
• rubber
• polyemryoni
• Project 10
• Session 1
• session 150--Restriction_Enzymes
• session 151-- Modification_Enzymes
• session 152-- Ligases_and_Nucleases
• session 153-- Use_of_Restriction_Enzymes
• session 154--Isochizomers
• Plasmid purification & Manipulation
• Classification of Vectors
• Cosmids
• session-1-Seed quality assesment-Importance_Varietalidentification through grow out test
• Session 2 -Seed Act 1966
• Session 2 -Seed Act 1966
• Session 4 - Seed Control Order 1983
• Session 5 -Duties and Powers of Seed Inspectors
• Session 2 -Seed Act 1966
• Session 4 - Seed Control Order 1983
• Session 6-Plants, fruits, and seeds order 1989
• Session 7 - The Protection of Plant Varieties and Farmer’s Rights
• Session 8 - National Seed Policy 2002
• Session 9 - Seed Bill 2004
• Session 10 - Amendments
• 180_Basics of protein structure
• cDNA Library
• Genetic libraries
• Transformation
• Vectors
• Session 201, MABC
• Session 208, Advanced breeding tools
• Session 215, Western blotting
• Session 209, RAD-Seq
• session 159 -- DNA Methylation
• Heterosis
• Cloning vectors- Plasmid-pUC 19, λ Phage
• Phagemid_and_M13_vector
• Bacterial_Artificial_Chromosomes
• Yeast_Artificial_Chromosomes
• P1_derived_artificial_chromosome
• SDS_PAGE
• Artificial Vectors_ Construction of pBR322
• Cloning vectors- Plasmid-pUC 19, λ Phage.
• Types_of_Expression_Vectors
• Utilization of heterosis in Agriculture and Horticultural crops
• session-2-Molecular and biochemical test_ Detection of genetically modified crops
• Threshing methods
• Session_ 3 Field inspection principles and methods- Inspection at harvesting, threshing and processing
• Techniques in Hybrid Seed Production (Emasculation and Crossing)
• Session_ 4 Pre-and post-processing quality testing of seed
• Session_ 5 Seed testing concepts and objectives, its role in seed quality control
• Video: Types of PCR, cDNA Synthesis and RT-PCR
• Seed drying and drying methods
• Session_ 7 Seed testing equipment’s
• Air distribution system and types of seed dryers for heated air-drying
• Video: Multiflex PCR
• Session_ 8 Quick viability test and seed standards
• Self Incompatibility and its use
• Self Incompatibility and its use
• Seed cleaning and methods of seed cleaning
• Session_ 9 Seed vigour-its significance and testing methods
• Session_ 10 Post-harvest inspection - processing, bagging and tagging
• Air distribution system and types of seed dryers for heated air-drying
• Heterosis
• Air distribution system and types of seed dryers for heated air-drying
• Utilization of heterosis in Agriculture and Horticultural crops
• Techniques in Hybrid Seed Production (Emasculation and Crossing)
• Session_ 1 Seed storage-principles of seed storage 1
• Male sterility and its use in hybrid seed production
• Session_ 2 Factors affecting seed longevity during storage
• Development and maintance of A, B and R line
• HYBRID SEED PRODUCTION OF RICE, SORGHUM, BAJRA
• Seed production in sunflower and cotton
• Session_ 3 Seed treatments
• Seed Production In Vegetables
• Session_ 4 Packaging materials during storage
• Session_ 5_Measures for pest and disease control during storage and godown sanitation
• Seed processing equipment- cleaner cum grader
• Video: Transcriptomics
• Seed processing equipment- cleaner cum grader
• Video: Pyrosequencing
• Video: Application of PCR
• Heterosis
• Utilization of heterosis in Agriculture and Horticultural crops
• Techniques in Hybrid Seed Production (Emasculation and Crossing)
• Self Incompatibility and its use
• Male sterility and its use in hybrid seed production
• HYBRID SEED PRODUCTION OF RICE, SORGHUM, BAJRA
• Development and maintance of A, B and R line
• HYBRID SEED PRODUCTION OF RICE, SORGHUM, BAJRA
• Seed production in sunflower and cotton
• Seed Production In Vegetables
• session 85 seed quality
• 75
• 76
• 77
• Upgrading equipment working principles- specific gravity separator, colour sorter and indented cylinder separator
• 78
• 79
• 80
• 81
• 82
• Upgrading equipment working principles- spiral separator, magnetic separator and disc separator
• session 85 seed quality
• 83
• Seed quality enhancement technique- seed treatment
• 84
• 82
• 81
• 80
• 79
• 78
• Seed quality enchancement-seed invigouration technique
• 77
• 76
• 75
• Seed treatment equipment
• 75
• 76
• Heterosis
• 1 Floral structure
• 2 pollination Mechanisms
• 3 Microsporogenesis
• 4 Megagametogenesis
• 4 Megagametogenesis
• 4 Megagametogenesis
• 5 Male sterility
• 5 Male sterility
• 5 Male sterility
• 6 Embryo Development
• 6 Embryo development
• 7 Endosperm development
• 8 Apomixix
• 9 embryo rescue
• 10 Synthetic seeds
• 10 Synthetic seeds
• 9 embryo rescue
• 9 embryo rescue
• 9 embryo rescue
• session 86 floral biology and seed production RICE
• session 87 floral biology of wheat
• session 88 FLORAL STRUCTURE AND BREEDING MECHANISM IN MAIZE
• session 89 floral biology of pearl millet
• session 90 FLORAL BIOLOGY COWPEA
• session 91 floral biology of chickpea
• session 92 FLORAL BIOLOGY OF CASTOR
• session 93 floral biology of mustard
• session 94 FLORAL BIOLOGY OF COTTON
• Session_ 10 WTO and OECD in seed trade_
• Session_ 9 International seed movement_ Role of international organizations
• Session_ 8 Factors affecting seed marketing and demand
• Session_ 8 Factors affecting seed marketing and demand
• Session_ 6 Seed marketing-structure and organization
• Session_ 5_Measures for pest and disease control during storage and godown sanitation
• Session_ 3 Seed treatments
• Session_ 2 Factors affecting seed longevity during storage
• session-1-Seed quality assesment-Importance_Varietalidentification through grow out test
• session-2-Molecular and biochemical test_ Detection of genetically modified crops
• Session_ 3 Field inspection principles and methods- Inspection at harvesting, threshing and processing
• session 156--Homopolymer Tailing_2
• session 158-- Immunodetection_2
• session 155--Ligation Procedure and Application_3
• session 157-- Linkers and DNA Adapters_4
• Gene transfer in plants
• Transgenic Plants.MP4
• Application of transgenic plants Case study I
• Transgenic plants case study II.MP4
• Traqnsgenic rice case study III
• Session_ 4 Pre-and post-processing quality testing of seed
• Session_ 5 Seed testing concepts and objectives, its role in seed quality control
• Session_ 7 Seed testing equipment’s
• Session_ 8 Quick viability test and seed standards
• Session_ 9 Seed vigour-its significance and testing methods
• Session_ 10 Post-harvest inspection - processing, bagging and tagging
• Session 3 - Seed Rules
• 190 Intro Molecular Marker GPL
• 190 Introduction to Molecular:Genetic Markers
• 191 Hybridization based Markers GPL
• 191 Hybridization and PCR based Markers GPL
• 192 193 Types of Molecular Markers and it's applications GPL
• 192 Markers Types and Applications 193
• 194 Sequence based molecular markers - SNPs GPL
• 194 Sequence based molecular markers SNPs
• 195 Molecular Marker with advantages and limitations - RFLPs GPL
• 195 Molecular Marker with advantages and limitations - RFLPs
• 196 Molecular Marker with advantages and limitations -RAPD GPL
• 196 Molecular Marker with advantages and limitations -RAPD
• 197 Sequence based molecular markers SSRs GPL
• 197 Molecular Marker with advantages and limitations -SSR
• 198 Molecular Marker with advantages and limitations –AFLP GPL
• 198 Molecular Marker with advantages and limitations –AFLP
• 199 Genetic Markers Case Study
• 199 Molecular Markers Case Study GPL
• 217 Check the hybrid (F1) fidelity using molecular markers GPL
• 217 Hybrid Fidelity using Molecular Markers
• session 85 seed quality
• 180_Basics of protein structure
• cDNA Library
• Genetic libraries
• Transformation
• Vectors
• Genetic libraries
• cDNA Library
• Transformation
• Session 200, MAS
• Session 201, MABC
• Session 208, Advanced breeding tools
• Session 209, RAD-Seq
• Session 215, Western blotting
• GWAS-case study
• GWAS (2)
• QTL-case study
• Self Incompatibility and its use
• Male sterility and its use in hybrid seed production
• Development and maintance of A, B and R line
• HYBRID SEED PRODUCTION OF RICE, SORGHUM, BAJRA
• Seed production in sunflower and cotton
• Seed Production In Vegetables
• Self Incompatibility and its use
• Pre requisites for hybrid seed production
• Introduction to seed production
• Deterioration of variety
• Hybrid seed production
• Male sterility and self incompatibility
• Seed multiplication ratios seed replacement rate demand and supply suitable areas of seed production and storage
• Agronomy of seed production and agroclimatic zones
• Generation system of seed multiplication
• Life span of a variety and causes for its deterioration_ certification standards for self and cross pollinated and vegetative propagated crops.
• Life span of a variety and causes for its deterioration_ certification standards for self and cross pollinated and vegetative propagated crops.
• Planning of seed production for different classes of seeds for self and cross pollinated crops
• Planning of seed production for different classes of seeds for self and cross pollinated crops
• Seed quality control system and organization, seed village concept; Seed production agencies, seed industry and custom seed production in India
• Session 105 Introduction to methods of propagation
• Session 105 Introduction to methods of propagation
• Session 106 Modes of reproduction
• Session 107 Technology for quality seed production in important flower crop chrysanthemum
• Session 108 Technology for quality seed production in important flower crop carnation
• Session 109 Seed production technology of annual medicinal plants Senna
• Session 110 classification based on reproduction and pollination behaviour in Papaya
• Session 111 Classification based on reproduction and pollination behaviour Peach
• Session 112 Plantation Crops role of seed in perennial plant species classification based on reproduction and pollination behaviourRubber
• Session 113 Polyembryony and its significance
• Session 114 Clonal propagation and multiplication in tropical, sub-tropical and temperate fruits and plantation crops
• Session 133, PCR principle - video 1
• Session 163, PCR-Methodology - Video 2
• Seed Quality assessment_vdo
• Mutation Breeding
• MAS in biotic stress resistance video
• MAS in abiotic stress resistance-Video
• Session 160--DNA hybridization and Cot curve Mp4
• Session- 161-- DNA seq sanger method Mp4
• Session 164--PCR_Primer_Design.Mp4
• Practice secation I
• Practice Section-II
• Practice Section-I
• Practice Section-II
• Practice 1 Emasculation methods
• Practice 2 Male sterility
• Practice 2 Male sterility
• Threshing , drying and cleaning
• Germination, treatment and packing
• Practice secation I
• Threshing , drying and cleaning
• Germination, treatment and packing
• Practice session 1 Types of Heterosis and prediction of Performance of double cross hybrids
• Practice session 2 Different Methods of Emasculation Techniques
• Practice I classification based on reproduction and pollination behaviour in Papaya
• Practice I classification based on reproduction and pollination behaviour in Papaya
• Practice II Introduction to methods of propagation
• Seed quality- practice session
• floral biology of castor- practice session
• 1
• http://courseware.cutm.ac.in/wp-content/uploads/2021/02/1-4.mp4
• 1
• 1
• 1
• 1
• 1
• 1
• Floral Biology & seed development and morphology and Modes of propagation in vegetables
• Practice Session-I
• 3
• 4
• 5
• 6
• 7
• 8
• 9
• 10
• Practice Session-I
• Practice Session-II
• QTL-case study
• QTL-case study