Agricultural Microbiology


Ms. Sudeepta Pattanayak


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Course Name : Agricultural Microbiology

Code(Credit) : ASAM1201(1-1-0)

Course Objectives

  • To gain knowledge on several beneficial and harmful micro-organisms
  • To have detailed idea about their cell structure, reproduction and mechanism of action
  • To know the complex interaction between agriculture system and micro-organism.
  • To introduce micro-organism in agricultural system for building a pathway for sustainable agriculture

Learning Outcomes

  • Learners will gain detailed idea on various micro-organisms, its harmful or beneficial effects on Agriculture, how to use them in a safer way for creating a better agriculture system like using as biofertilizer, biopesticide, Silage production, plant disease management etc.

Course Syllabus

Introduction.Microbial world: Prokaryotic and eukaryotic microbes. Bacteria: cell structure, chemoautotrophy, photo autotrophy, growth. Bacterial genetics: Genetic recombination- transformation, conjugation and transduction, plasmids, transposon.
Role of microbes in soil fertility and crop production: Carbon, Nitrogen, Phosphorus and sulphur cycles. Biological nitrogen fixation- symbiotic, associative and aysmbiotic. Azolla, blue green algae
and mycorrhiza.Rhizosphere and phyllosphere. Microbes in human welfare: silage production, biofertilizers, biopesticides, biofuel production and biodegradation.

Introduction to microbiology laboratory and its equipments; Microscope- parts, principles of microscopy, resolving power and numerical aperture.Methods of sterilization.Nutritional media and their preparations.Enumeration of microbial population in soil- bacteria, fungi, actinomycetes.Methods of isolation and purification of microbial cultures.Isolation of Rhizobium from legume root nodule.Isolation of Azotobacter from soil.Isolation of Azospirillum from roots.Staining and microscopic examination of microbes.

Session Plan

Session 1

Introduction to microbial world

 Introduction to microbial world

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Session 2

Practice: Introduction to microbiology laboratory and its equipment

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Session 3

Session 4

Practice: Microscope- parts, principles

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Session 5

Session 6

Practice: Methods of sterilization

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Session 7

Session 8

Practice: Nutritional media and their preparations.

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Session 9

Session 10

Practice: Enumeration of microbial population in soil

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Session 11

Role of Microbes in soil fertility and crop production

 Role of microbes in soil fertility and crop production

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Session 12

Session 13

Practice: Methods of isolation and purification of microbial cultures

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Session 14

Session 15

Session 16

Practice: Isolation of Rhizobium, Azatobacter and Azospirillum from legume root nodule

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Session 17

Biofertilizer and Biopesticide

 Biofertilizer and biopesticide

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Session 18

Session 19

Rhizosphere and Phyllosphere

Rhizosphere and Phyllosphere

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Session 20

Session 21

Practice: Bacterial staining

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Question Bank

Reference Books

1. Microbiology. Pelczar, J.r.,M.J.E.C.S.Chan and Krieg, N.R.(5th Ed.) 2015. McGraw Hill Publishers, New York.
2. Microbiology. Prescott, L.M>, Harley, J.P. and Klein, D.A.(9th Ed.) 2014. McGraw Hill Publishing Ltd.,New York.
3. Brock Biology of Microorganisms. Madigan, M.,Martinko, J.M. and Parker, J. (14 Ed.) 2015. Prentice Hall of India Pvt. Ltd., New Delhi
4. Soil Microbiology: Subba Rao, N.S.(4th Ed.) 2014. Oxford & IBH Publishing Co.Pvt. Ltd.,New Delhi.
5. Microbiology A Laboratory Manual: James, C and Natile, S.(10th Ed.) 2014. Pearson India Education Services Pvt. Ltd., South Asia.
6. Experiments in Microbiology, Plant Pathology and Biotechnology, Aneja, K.R.2011. New Age International (P) Ltd., Publishers, New Delhi.

Case Studies

Case Studies

Our Main Teachers

Ms. Sudeepta Pattanayak

Assistant Professor, Dept of Plant Pathology

Ms. Sudeepta Pattanayak is currently working as Assistant Professor in Centurion University of Technology and Management since 2018. She has 2 years of research and teaching experience. In her research period, she has attended many national and international conferences and seminars. In addition to this, she has written many more research articles and book chapters. Besides […]