Agricultural Microbiology


Dr. Siddhartha Das


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Course Name : Agricultural Microbiology

Code(Credit) : ASAM1201(1-1-0)

Programme Outcomes

PO1 : Knowledge Enhancement : Demonstrate knowledge and understanding of essential facts, concepts, principles and theories relating to Soil Science, Agronomy, Horticulture, Genetics and Plant Breeding, Plant Pathology, Entomology and other such areas of agriculture.

PO2 : Professional Enhancement : Understanding the value and processes of life-long learning and professional development.

PO3 : Entrepreneurship Skills : Understanding the processes of setting up and managing viable business ventures.

PO4 : Communication & Extension Skills : Developing appropriate communication skills for effective transfer of knowledge and technologies through extension programs.

PO5 : Team Work : Play effective roles in multidisciplinary teams.

PO6 : Educational Leadership : Recognize and meet emerging agriculture challenges of global society in the 21st century and developing leadership and strong linkages in the agro-industrial setup.

PO7 : Data Handling : Collect, analyze and interpret scientific data.

PO8 : Ethics : Apply ethical principles and commit to professional ethics and responsibilities and norms of the scientific practice.

PO9 : Computational Skills : Recognize, analyze problems and plan strategies for their solutions with the help of computer skills.

Course Objectives

  • To gain knowledge on several beneficial and harmful micro-organisms
  • To have detailed idea about their cell structure, reproduction and mechanism of action
  • To know the complex interaction between agriculture system and micro-organism.
  • To introduce micro-organism in agricultural system for building a pathway for sustainable agriculture

Course Outcome

  • CO1 The student will comprehend the fundamentals of microbial structure and function and investigate the differences between prokaryotes and eukaryotes.
  • CO2  To understand the diverse physical and chemical conditions needed for bacterial development
  • CO3 Impart information on the creation of helpful bacteria.
  • CO4 Basic skilling of instrumentation, microbial culture handling and maintenance

Course Syllabus

Introduction.Microbial world: Prokaryotic and eukaryotic microbes. Bacteria: cell structure, chemoautotrophy, photo autotrophy, growth. Bacterial genetics: Genetic recombination- transformation, conjugation and transduction, plasmids, transposon.
Role of microbes in soil fertility and crop production: Carbon, Nitrogen, Phosphorus and sulphur cycles. Biological nitrogen fixation- symbiotic, associative and aysmbiotic. Azolla, blue green algae
and mycorrhiza.Rhizosphere and phyllosphere. Microbes in human welfare: silage production, biofertilizers, biopesticides, biofuel production and biodegradation.

Introduction to microbiology laboratory and its equipments; Microscope- parts, principles of microscopy, resolving power and numerical aperture.Methods of sterilization.Nutritional media and their preparations.Enumeration of microbial population in soil- bacteria, fungi, actinomycetes.Methods of isolation and purification of microbial cultures.Isolation of Rhizobium from legume root nodule.Isolation of Azotobacter from soil.Isolation of Azospirillum from roots.Staining and microscopic examination of microbes.

Session Plan

Session 1

Introduction to microbial world

 Introduction to microbial world

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Session 2

Practice: Introduction to microbiology laboratory and its equipment

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Session 3

Session 4

Practice: Microscope- parts, principles

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Session 5

Session 6

Practice: Methods of sterilization

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Session 7

Session 8

Practice: Nutritional media and their preparations.

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Session 9

Session 10

Practice: Enumeration of microbial population in soil

Watch youtube video

Session 11

Role of Microbes in soil fertility and crop production

 Role of microbes in soil fertility and crop production

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Session 12

Session 13

Practice: Methods of isolation and purification of microbial cultures

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Session 14

Session 15

Session 16

Practice: Isolation of Rhizobium, Azatobacter and Azospirillum from legume root nodule

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Session 17

Biofertilizer and Biopesticide

 Biofertilizer and biopesticide

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Session 18

Session 19

Rhizosphere and Phyllosphere

Rhizosphere and Phyllosphere

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Session 20

Session 21

Practice: Bacterial staining

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Question Bank

Reference Books

1. Microbiology. Pelczar, J.r.,M.J.E.C.S.Chan and Krieg, N.R.(5th Ed.) 2015. McGraw Hill Publishers, New York.
2. Microbiology. Prescott, L.M>, Harley, J.P. and Klein, D.A.(9th Ed.) 2014. McGraw Hill Publishing Ltd.,New York.
3. Brock Biology of Microorganisms. Madigan, M.,Martinko, J.M. and Parker, J. (14 Ed.) 2015. Prentice Hall of India Pvt. Ltd., New Delhi
4. Soil Microbiology: Subba Rao, N.S.(4th Ed.) 2014. Oxford & IBH Publishing Co.Pvt. Ltd.,New Delhi.
5. Microbiology A Laboratory Manual: James, C and Natile, S.(10th Ed.) 2014. Pearson India Education Services Pvt. Ltd., South Asia.
6. Experiments in Microbiology, Plant Pathology and Biotechnology, Aneja, K.R.2011. New Age International (P) Ltd., Publishers, New Delhi.

Contents revised

  1. Fungal staining technique
  2. Ooze test
  3. Study of microbial growth under different pH range

Course Outcome (CO) - Program Outcome (PO) Mapping

  PO1 PO2 P03 P04 P05 P06 P07 P08 P09
CO1 2 2 1 2 2 2 2 2 1
CO2 2 2 2 1 2 2 2 2 1
CO3 2 2 2 2 2 2 2 2 2
CO4 2 2 2 2 2 2 2 2 2
Avg of CO-PO affinity levels 2.00 2.00 1.75 1.75 2.00 2.00 2.00 2.00 1.50


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