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Diploma Track Title : Nutraceuticals

Diploma Track Title : Nutraceuticals

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Preetha Bhadra

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Diploma Courses

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Diploma Track Title : Nutraceuticals

Track Total Credits ( T-P-P): 3-6-15 (24 Credits)

 

Type of course: Diploma Course Track and Practice

Eligibility: 10th/12th/ITI

Pre-requisites: Participants would have some idea regarding the basics of biology and interest in the field of nutrition.

Duration of the course:

Class: 120 hours of classes

Practical: 90 hours (Practice to be done on campus and outside campus)

Project: 120 hours of project work

Key Highlights

  • Participants will get a certificate from the university after successful completion.
  • Participants can work on different medicinal plant extracts
  • Participants can work on different drying mechanism of the food
  • Participants can have an industrial experience of the CO2 extraction plant
  • Participants can also have experience working in both plant and animal models.
  • Imparts knowledge for development as an entrepreneur 

Course Objectives

  • To study the advantages of functional foods over conventional Medicine to avoid potential side-effects
  • To Study dietary supplements
  • To Study about food gene interface
  • To distinguish between food, functional food, and supplements
  • To learn about Drug Designing,
  • To learn about detailed Protein structure, pharmacophore, basics of Docking and Discovery studio and application in Agriculture, Fisheries and others Science.

Learning Outcomes

  • To understand Functional foods and their effects on human health
  • To understand the role of antioxidants, polyphenols, omega-3 fatty acids, to prevent different physiological disorders.
  • To Understand the importance of personalized food with respect to nutrigenetics.
  • To be able to design a drug molecule and identify its applications
  • To be able to analyze nanomaterials and use it as Bio-Pesticides and Biofertilizers,
  • To be able to design a drug molecule and identify its applications.
  • To be able to illustrate the structure of Protein, Pharmacophores

Course Division

Track courses (3-6-0)

  1. Introduction to Nutraceutical (1+2)
  2. Functional Food (1+2)
  3. Nutrigenetics (1+2)

Project Courses (0-6-9)

Course Syllabus:

Track Course 1: Introduction to Nutraceutical (1+2)

Theory:
Organizational elements, classification of nutraceuticals, dietary supplements, fortified foods, functional foods, and Phyto-nutraceuticals. The scope involved in the industry, Indian, and global scenario, Food Chemistry, Preservatives, food colors.

Practicals:

  1. Milk Analysis: Determination of fat, acidity, lactose, protein, adulterants
  2. Analysis of water: Collection of the sample, preliminary examination, Total soluble solids, Determination of total hardness, chlorides, dissolved oxygen
  3. Analysis of fats: acid value, saponification value, iodine number, antioxidants
  4. Analysis of foods: Determination of reducing and non reducing sugar, protein, determination of ash/total protein/moisture in dietary fibers
  5. Anti-nutritional Factors: Trypsin inhibitor, Phytic acid, Antifungal activity of cystatin
  6. Vitamins: Ascorbic acid by colorimetric method, estimation of Vit A/E
  7. Pigments: Curcumin, chlorophyll, carotene
Track Course 2: Functional Food (1+2)
Theory:
  • Prebiotics-   Definition, chemistry, sources, metabolism and bioavailability, the effect of processing, physiological effects, effects on human health, and potential applications in risk reduction of diseases. perspective for food applications for the – Non-digestible carbohydrates/oligosaccharides, Dietary fiber, Resistant starch, Gums.
  • Probiotics- Taxonomy and important features of probiotic microorganisms. Health effects of probiotics including mechanism of action. Probiotics in various foods: fermented milk products, non-milk products, etc. Quality Assurance of probiotics and safety.
  • Drug Discovery - Issues, Target and Lead Identification, Drug And Databases, Drug Properties, Drug Solubility/permeability, ADME, Molecular Modelling, Quantitative Structure-Activity Relationship, Target-based drug design, Docking. Pharmacokinetics/ pharmacodynamics, Discussion on Molecular Drug Designing, Targeted drug designing, Application

Practicals:

  1. Extraction Techniques: Soxhlet Extraction, Cold Press Extraction, Super Critical Fluid Extraction, CO2 Extraction
  2. Isolation and Purification of Phytochemicals: TLC, Paper Chrom, Column Chrom, HPLC, Analysis of Aflatoxin
  3. Validation of Phytochemicals: Using different microorganisms and mammalian cell lines

Track Course 3: Nutrigenetics (1+2)

Theory:

Introduction to Nutrigenetics, Nutrigenomics, Concept of Personalized food with special references to athletes. Introduction to different types of public domain databases, data mining strategies, primer designing.

Practical:

  1. m-RNA extraction from treated mammalian cell line
  2. Preparation of c-DNA
  3. Study of Gene expression in Real-Time PCR
  4. Electrophoresis

Text Books

Introduction to Nutraceuticals:

  • Giuseppe Mazza; Functional Foods: Biochemical and Processing Aspects, Volume 1; CRC Press
  • Robert E.C. Wildman; Handbook of Nutraceuticals and Functional Foods, Second Edition; CRC Press
  • Massimo Maffei; Dietary Supplements of Plant Origin; CRC Press
  • Fereidoon Sahidi, Deepthi K. Weerasinghe; Nutraceutical Beverages, Chemistry, Nutrition and Health Effects; American Chemical Society

Functional Foods:

  • Ronald R. Watson; Vegetables, Fruits, and Herbs in Health Promotion; CRC Press
  • Fruit and Cereal Bioactives: Sources, Chemistry and Applications; ÖzlemTokusoglu; Clifford Hall III; CRC Press
  • Susan Sungsoo Cho, Mark L. Dreher; Marcel; Dekker Handbook of Dietary Fibre

Nutrigenetics:

  • Principles of Nutrigenetics and Nutrigenomics - 1st Edition. Print Book & E-Book. ISBN 9780128045725, 9780128045879.
  • Nutrigenetics: Applying the Science of Personal Nutrition. Academic Press; Reprint edition (19 August 2016).
  • Nutrigenetics and Nutrigenomics. World Review of Nutrition and Dietetics, Vol. 93

Development of Personalized Food and Medicine

Textbook of Personalized Medicine. Authors: Jain, K. K. Springer Publication

Development of Biopesticides and Biofertilizers

Biofertilizers and Biopesticides Paperback – 29 June 2019 by Krishnendu Acharya (Author), Surjit Sen (Author), Manjula Rai (Author)

Biofertilizer and Biopesticide Hardcover – 1 March 2011, by Shalini Suri (Author)

Development of Immune Boosters

Immune System: How To Boost The Immune System Naturally by Elizabeth Grace

Session Plan for Track courses

Session Plan:  1.1.1

Classification of nutraceuticals

classification of nutraceuticals

classification of nutraceuticals

Session Plan:  1.1.2

Phyto-nutraceuticals

Phyto-nutraceuticals

Phyto-nutraceuticals

Session Plan:  1.1.3

Food Colors

Food colours

Food colours

Session Plan:  1.2.1

Milk Analysis: Determination of fat, acidity, lactose, protein, adulterants

Session Plan:  1.2.2

Analysis of water: Collection of the sample, preliminary examination, Total soluble solids, Determination of total hardness, chlorides, dissolved oxygen

Session Plan:  1.2.3

Analysis of fats: acid value, saponification value, iodine number, antioxidants

Session Plan:  1.2.4

Analysis of foods: Determination of reducing and non-reducing sugar, protein, determination of ash/total protein/moisture in dietary fibers

Session Plan:  1.2.5

Anti-nutritional Factors: Trypsin inhibitor, Phytic acid, Antifungal activity of cystatin

Session Plan:  2.1.1

Probiotics &Prebiotics-Definition, chemistry, source

Functional food

Functional food

Session Plan:  2.1.2

Perspective for food applications for the – Non-digestible carbohydrates/ oligosaccharides, Dietary fiber, Resistant starch, Gums

Mechanism of action of Prebiotics

Probiotic Introduction

Session Plan:  2.1.3

Probiotics- Taxonomy and important features of probiotic microorganisms

Probiotic Foods

TYPES OF PREBIOTICS

Session Plan:  2.1.4

Different types of Prebiotics

TYPES OF PREBIOTICS

Role of Functional Food

Session Plan:  2.2.1

Extraction Techniques

 

Soxhlet Extraction

 

Cold Press Extraction

Session Plan:  2.2.2

Isolation and Purification of Phytochemicals

 

TLC

 

Column Chromatography

Session Plan:  3.1.1

Introduction to Nutrigenetics

Nutragenetics

Nutragenetics

Session Plan:  3.1.2

Introduction to Nutrigenomics

Nutrigenomics

Nutragenetics

Session Plan:  3.1.3

Human Genome Project

Human Genome Project

Human Genome Project

Session Plan:  3.1.6

Genes associated with metabolic disorders

Metabolic Disorder

Metabolic Disorder

Session Plan:  3.2.1

m-RNA extraction from treated mammalian cell line

Session Plan:  3.2.2

Preparation of c-DNA

Session Plan:  3.2.3

Study of Gene expression in Real Time PCR

Session Plan:  3.2.4

Electrophoresis

Session plan for Project Courses: (Any two)

2.1. To develop personalized food and beverages rich in Antioxidants and Polyphenols

This project includes the extraction of phytochemicals, identification of bioactive compound(s), isolation of this compound, and incorporation in food/beverages. This food/beverages will be specific for people suffering from hyperglycemia, obesity, or may target athletes to provide instant energy.

2.2. To develop low-cost omega-3 capsules from fish oil/ walnuts

PUFAs like omega-3 fatty acids found in fish oil, walnuts can reduce inflammation and improve hypertriglyceridemia. Formulation of this type of capsule involves drug designing (using Discovery Studio, Biovia) and clinical trials.

2.3. To develop immune boosters

Immune boosters are most suitable for children below 10 years and the aged person above 60 years. This will strengthen the internal immunity of the body and will help to combat the common cold viruses, cases of flu, etc.

2.4. To develop protein bars

Protein bars are popular among the young generation as they are healthy and tasty. Low-calorie protein bars, rich with fruits and nuts may be a popular product with high market value. The level of carbohydrate, fats, proteins, vitamins need to be standardized and hence basic food biochemistry analysis will be involved.

2.5. To develop the extraction of antioxidants and polyphenols from different sources

SCFE (using carbon dioxide) of antioxidants and polyphenols from different sources (eg: Ramie, Dragon Fruit, Berries) can be performed in Gram Tarang Foods Pvt. Ltd. Besides, soxhlet, cold press methods can be explored for finding a commercial way of extraction and supplying it to different food/beverage companies. This project could be beneficial for getting the monetary benefits as well as getting publication by studying the biological efficacy of the plant extracts.

2.6. In-silico Validation of the Phytochemicals on Fish cell line and Plant Microbes
The bioactive molecules from phytochemicals can be used against different types of plant pathogens as well as can be used as fish feed to protect from bacterial or fungal action. This will include molecular docking, pharmacophore modelling. In- silico models will be validated using wet lab tests. Quality publications are expected.

2.7. Drug Designing
Known bioactive molecules from natural resources will be used for drug designing against different human physiological disorders, such as, Hypertension, Hyperglycemia, Parkinson’s disease and certain metabolic disorders. Validation of the drugs warrants clinical trial and may lead to patent and publications.

2.8. In-silico Validation of Nanoparticle dosages on Fish Cell Line and Plant Microbes
Studies on nanoparticles and its applications in plant pathology and fishery sciences are gaining immense popularity. In-silico models will can provide proper experimental design to many research works related to fisheries and plant pathology that would save time, money and man power. Validation of in-silico data in wet lab may lead to quality publications.

Assessment

15% marks for two assignments

20% marks for one internal test

10% marks for one quiz test

30% marks for two practical tests

20% marks for final exam

5% marks for attendance

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    Preetha Bhadra

    Assistant Professor
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    PhD in Biophysics, currently working as an Assistant Professor in the Department of Biotechnology. My current working field is the uses of different Medicinal plants and spices and their application in Agriculture and Lifesciences, Drug designing. I love to take challenges and work hard to complete that. New domains and areas of my venture are […]

    Present Position Assistant Professor, Dept. of Biochemistry and Plant Physiology, MSSSoA, Centurion University of Technology & Management, Odisha, India Mobile: 9330492088 , E-mail: pradipta.banerjee@cutm.ac.in Education Bachelor of Science (Biotechnology), PES College, Bangalore University, Karnataka, India Master of Science (Biochemistry), Vidya Sagar University, West Bengal, India Ph.D. (Soil Sc. & Agri. Chemistry), Visva Bharati University, Birbhum, […]