SKILL: Medical Diagnostic Techniques


Susmita Chakrabarty


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Course Name : SKILL: Medical Diagnostic Techniques

Code(Credit) : CUTM3124(0-3-1)

Course Objectives

  • Demonstrates proper handling of patients/specimens and evaluates situations that may cause adverse issues in the diagnostic division.
  • Demonstrate skill with the microscope, centrifuge, and other laboratory equipment.
  • Basic understanding and analysis of the excretory products and other body fluids.
  • Basic understanding of hematology, immunology, clinical biochemistry, and microbiology with emphasis placed on point of care testing in all areas of the laboratory.

Learning Outcomes

  • Able to collect the pathological specimen.
  • They can Preserve and process the pathological sample.
  • Able to handle all laboratory instruments.
  • Able to detect the abnormal conditions.

Course Syllabus

Practice I: Fundamentals of Medical Diagnostic Techniques

  • Operation of Medical laboratory equipment and its installation, calibration, quality control.
  • General consideration on specimen collection in clinical laboratory. Pre-analytic (pre-examination), analytic (examination), and post-analytic (post-examination).

Practice II: Routine Hematological Investigations

  • Complete Blood Count (DLC, TLC, TPC, Hb, PCV) and its clinical significance.
  • Bleeding time and Clotting time.
  • Blood grouping and Rh factor.
  • Peripheral smear study.
  • Blood Parasites.

Practice III: Biochemical investigation with KIT methods

  • Diabetes and Blood glucose analysis (GOD/POD).
  • LFT (SGOT, SGPT, ALP, Albumin, Bilirubin total and direct, Total Protein).
  • KFT/RFT (Urea, Uric acid, Creatinine, K+, Na+).
  • Lipid Profile (Triglycerides, Cholesterol, HDL, LDL, VLDL).

Practice IV: Routine investigation in clinical pathology


  • Urine RE/ME
  • Stool microscopic examination
  • Semen analysis
  • Sputum for AFB and basic of DOT, MycoDot, Mantoux.



Practice V: Routine investigation in Microbiology


  • Sterilization: Hot Air Oven, Autoclave, Incubator
  • Staining Technique: Gram stain, Zn Stain
  • Immunochromatographic Test Technique (kit method): MPICT, HIV, HBsAg, HCV, Widal test (Typhoid), TOXO (Toxoplasma), Dengue, Chikungunya.
  • Media Preparation: (Blood agar, Chocolate Agar, CLED (Cysteine- Lactose Electrolyte Deficient) agar, MLA (MacConkey Lactose Agar), MHA (Muller Hinton Agar), MSA (Mannitol Salt Agar), XLD (Xylose Lysine Deoxy cholate), BEA (Bile Esculin Agar), PPA (Phenylalanine Agar), TSI (Triple Sugar Iron), Nutrient Broth, Peptone water, BHI Broth.
  • Culture & Isolation process of bacteria



  1. Study on common cause of Anemia, laboratory investigation and treatment.
  2. Sickle cell anemia & its types, common cause, laboratory investigation and treatment.
  3. Study on common Sexually Transmitted Disease (STD) its laboratory diagnosis and treatment.
  4. Study on hormonal diseases and it’s laboratory diagnosis.
  5. Objective & importance of different Department of clinical laboratory.
  6. Advantage and disadvantages of Manual and automation technique in Medical Laboratory.
  7. Enumerate regulation of medical lab Laboratory and the criteria of accreditation.



Suggested Readings:


  1. Kuby's Immunology (7th Ed) - by J. Owen, J. Punt, S. Strandford. Macmillan Higher Education, England.

(e-book link: )

  1. Prescott, Harley, and Klein’s Microbiology (Seventh Edition)- by Joanne M. Willey, Linda M. Sherwood, Christopher J. Woolverton. McGrawHill.
  2. Text book of Microbiology (7th Edition)- by Ananthanereyan & Paniker, Publisher Universities press.

(e-book link: )

  1. Fundamentals of Molecular Virology (2nd Edition)- by Nicholas H. Acheson, Wiley Publishers.

(e-Book-link: )

  1. Text book of Medical Laboratory Technology by P. B. Godker, PublisherBhalani.
  2. Bruce Alberts, Alexander Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter (2015) Molecular Biology of the cell, 6th edition, Taylor and Francis Group.



Online Tutorial links:


  1. Fundamentals of Immunology: Innate Immunity and B-Cell Function

(Coursera link: )

  1. Fundamentals of Immunology: T Cells and Signaling

(Coursera link: )

  1. Fundamentals of Immunology: Death by Friendly Fire

(Coursera link: )

  1. Viruses & How to Beat Them: Cells, Immunity, Vaccines

(edX link:

  1. The Immune System: New Developments in Research


  1. DNA Decoded (coursera link:
  2. DNA Replication (Lecturio link: )
  3. Transcription (Lecturio link:


Session Plan

Session 1

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Session 2

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Case Studies

Case Studies

Our Main Teachers

Susmita Chakrabarty is currently working as an Assistant Professor in the School of Paramedics and Allied Health Sciences at Centurion University of Technology and Management. Her specialization area includes the field of Medical Laboratory Sciences. At CUTM, she is engaged in teaching subjects like Hematology, Histology, Instrumentation and Technique, Analytical Biochemistry & Clinical Biochemistry, etc. […]