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Module-I (Animal Cell and tissue culture technology-I): 8 Hrs
Overview of animal cell and tissue culture technology; Cell culture laboratory design and basic laboratory equipments; Media preparation, Role of important components of culture media; Common laboratory hazards and safety issues to consider in cell culture laboratory
Practice 1: Sterilization techniques used in animal cell culture
Practice 2: Preparation of media for animal cell culture
Module-II (Animal Cell and tissue culture technology-II): 8 Hrs
Types of animal cell culture; Characterization and preservation of animal cells; Advances in cell culture technology; Opportunities and challenges in animal cell and tissue culture technology
Practice 3: Study of primary cell culture technique using chick embryo
Practice 4: Animal cell batch culture technique
Module-III (Transgenesis for livestock improvement-I): 4 Hrs
Overview of transgenic technology; Biopharming through animal transgenesis; Methods of producing transgenic farm animals; Identification and transfer of gene influencing better production and disease resistance
Module-IV (Transgenesis for livestock improvement-II): 5 Hrs
Gene transfer methods in animals: Microinjection, Retrovirus mediated gene delivery, Embryonic stem cell mediated gene transfer; Knockout model systems & their utility; Animal as bioreactor
Module-V (Assisted reproduction biotechnology for livestock improvement): 4 Hrs
Reproduction biotechnologies and their use in livestock; Somatic cell nuclear transfer cloning; In Vitro Fertilization, Embryo production, preservation and transfer; Sperm and embryo sexing; Intracytoplasmic sperm injection (ICSI); Cryopreservation and gamete banking
Module-VI (Animal Production technology for sustainable agriculture and food security): 7 Hrs
Polyculture of fish for high yield; Edible oyster production; Pearl oyster production; Vermi-culture and vermicomposting for alternative and sustainable agriculture; Fish culture in flow through system and recirculation technology
Practice 5: Vermicompost preparation from plant debris, cattle dung and paper waste
Module-VII (Animal Biotechnology & human health): 6 Hrs
Recombinant therapeutics and production of pharmaceuticals; Production of tissues and organs for humans and xenotransplantation; Process of gene therapy, Pros and cons in gene therapy; Retrovirus and adenovirus mediated gene therapy
Practice 6: Preparation of competent cell (Calcium chloride treatment method)
Text Books:
Reference Books:
Overview of animal cell and tissue culture technology
Video link: https://www.youtube.com/watch?v=RpDke-Sadzo
PPT link: https://www.slideshare.net/nagendragowda143/animal-cell-culture-techniques
Cell culture laboratory design and basic laboratory equipments
Video link: https://www.youtube.com/watch?v=CA5fVLK5zAE
PPT link: https://www.slideshare.net/SubhalaxmiSwain1/equipments-used-in-animal-cell-culture
Media preparation, Role of important components of culture media
Video: https://www.youtube.com/watch?v=cneascR3OEc
PPT: https://www.slideshare.net/RIZWANABBAS3/animal-cell-tissue-culture
Common laboratory hazards and safety issues to consider in cell culture laboratory
Video: https://www.youtube.com/watch?v=9eE9grrj3rI
Sterilization techniques used in animal cell culture
Preparation of media for animal cell culture
Types of animal cell culture
Video: https://www.youtube.com/watch?v=BDKf80J-rg4
PPT: https://www.slideshare.net/nadiamohdkp/types-of-cell-culture
Characterization and preservation of animal cells
Video: https://www.youtube.com/watch?v=5qXpvcaKFz4&t=30s
PPT: https://www.slideshare.net/varijnayan1/characterization-of-cell-line
https://www.slideshare.net/richatiwari54/cryopreservation-88695759
Advance techniques in animal cell culture
Video: https://www.youtube.com/watch?v=G1n3BgmcVMA
PPT: https://www.slideshare.net/rajpalchoudharyjat/advanced-techniques-in-animal-cell-culture
Opportunities and challenges in animal cell and tissue culture technology
Video: https://www.youtube.com/watch?v=w936wbss-5A
Study of primary cell culture technique using chick embryo
Animal cell batch culture technique
Overview of transgenic technology
Video: https://www.youtube.com/watch?v=8HTjul752IY&t=93s
PPT: https://www.slideshare.net/hazz12/transgenic-animal-technology-ppt
Biopharming through animal transgenesis
Video: https://www.youtube.com/watch?v=d6m3SoKIStQ
PPT: https://www.slideshare.net/kskuldeep1995/biopharming-molecular-farming
Methods of producing transgenic farm animals
Video: https://www.youtube.com/watch?v=RzYhcXjksKc
PPT: https://www.slideshare.net/MohsenAli2/production-of-transgenic-farm-animals
Identification and transfer of gene influencing better production and disease resistance
Gene transfer methods in animals: Microinjection
Gene transfer methods in animals: Retrovirus mediated gene transfer
Video: https://www.youtube.com/watch?v=G_NU3yXmmyI
PPT: https://www.slideshare.net/maryamshakeel792/retro-48281175
Gene transfer methods in animals: Embryonic stem cell mediated gene transfer
Video: https://www.youtube.com/watch?v=CSEToZEhpo8
PPT: https://www.slideshare.net/DhavalChaudhary7/transgenesis-in-animals
Knockout model systems & their utility
Animals as bioreactor
Web link: https://pubmed.ncbi.nlm.nih.gov/11131009/
PPT: https://www.scribd.com/presentation/278105263/Animal-as-Bioreactors
Reproduction biotechnologies and their use in livestock: Somatic cell nuclear transfer cloning
In vitro Fertilization, Embryo production, preservation and transfer
Video: https://www.youtube.com/watch?v=uXsCngh89fI&t=150s
PPT: https://www.slideshare.net/onlyuforu3/in-vitro-fertilization-37307905
Sperm and embryo sexing, Intracytoplasmic sperm injection (ICSI)
Video: https://www.youtube.com/watch?v=gJJEvuzeess&t=21s
https://www.youtube.com/watch?v=_krJsK5Dxj4
PPT: https://www.slideshare.net/AbdulJabbarAlkhazraji/embryo-sexing-pppt
Cryopreservation and gamete banking
Video: https://www.youtube.com/watch?v=71VJucLp8y8
PPT: https://www.slideshare.net/vksprasath/cryopreservationsperm-bankingartificial-insemination
Edible oyster production
Video: https://www.youtube.com/watch?v=amUE_R2kFx8&t=120s
PPT: https://www.slideshare.net/nidhiyaraghu/edible-and-pearl-oysters
Pearl oyster production
Video: https://www.youtube.com/watch?v=yj5AaM5IAD4
PPT: https://www.slideshare.net/nidhiyaraghu/edible-and-pearl-oysters
Vermi-culture and vermicomposting for alternative sustainable agriculture
Video: https://www.youtube.com/watch?v=Ujy7CKNsWzk
PPT: https://www.slideshare.net/krishnaSethi1/vermicomposting-118274903
Fish culture in flow through system and recirculation technology
Video: https://www.youtube.com/watch?v=W6lDgj5YwZg&t=43s
PPT: https://www.slideshare.net/sush_p/recirculation-in-fish
Vermicompost preparation from plant debris, vegetable garbage, cattle dung and paper waste
Recombinant therapeutics and production of pharmaceuticals
Video: https://www.youtube.com/watch?v=CJR33bu5zFE
PPT: https://www.slideshare.net/berran/recombinant-therapeutic-proteins
Production of tissues and organs for humans and xenotransplantation
Video: https://www.youtube.com/watch?v=rq5k3da_UWk
PPT: https://www.slideshare.net/khehkesha/xenotransplantation-16315599
Process of gene therapy, Pros and cons in gene therapy
Video: https://www.youtube.com/watch?v=xOQFJJOBGM0
PPT: https://www.slideshare.net/anniemirza14/gene-therapy-58257727
Retrovirus and adenovirus mediated gene therapy
Video: https://www.youtube.com/watch?v=21nx9dRTP18&t=115s
https://www.youtube.com/watch?v=WTrkURar1ow
PPT: https://www.slideshare.net/ankurndri/viral-vectors-for-gene-transfer
Preparation of competent cell (Calcium chloride treatment method)
Sujit Kumar Mishra is working as Assistant Professor in the School of Applied Sciences, Centurion University of Technology and Management, Bolangir Campus, Odisha, India. He has completed his M. Sc. from School of Life Sciences, Sambalpur University, submitted his Ph. D. thesis in Sambalpur University, and awaiting award of the doctoral degree. He has awarded […]
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