Animal Biotechnology – CUTM 1452

Teacher

Dr Bikash Ranjan Sahu

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Subject Code - CUTM 1452

Credit - 3-1-0 (Theory-Practice-Project)

Course Objectives

  1. To understand the tools and techniques required for the animal cell culture.
  2. To integrate assisted reproductive biotechnology techniques in livestock development.
  3. To utilize animal production technologies for sustainable agriculture and food security.

Course Outcome

At the end of the course, the student should be able

COs Course Outcomes Mapping COs with POs

(High-3, Medium-2, Low-1)

CO1 To define basic laboratory equipment used for animal cell culture along with different types of cell cultures. PO1-3, PO6-1, PO9-2
CO2 To understand techniques, involve in creating transgenic animals and biopharming. PO1-3, PO5-2, PO9-3
CO3 To differentiate among various gene transfer methods such as retrovirus mediated gene delivery, Embryonic stem cell mediated gene transfer and microinjection. PO1-3, PO9-2
CO4 To analyze animal production technologies for sustainable agriculture and food security. PO1-3, PO9-2
CO5 To apply animal biotechnology in human health such as recombinant therapeutics and production of pharmaceuticals. PO1-3, PO9-2

Course Outline

Module-I (Animal Cell and tissue culture technology-I): 8 H

Theory: Overview of animal cell and tissue culture technology; Cell culture laboratory design and basic laboratory equipment; Media preparation, Role of important components of culture media; Common laboratory hazards and safety issues to consider in cell culture laboratory.

Practice 1: Sterilization techniques used in animal cell culture.

Practice 2: Preparation of media for animal cell culture.

Module-II (Animal Cell and tissue culture technology-II): 8 H.

Theory: Types of animal cell culture; Characterization and preservation of animal cells; Advances in cell culture technology; Opportunities and challenges in animal cell and tissue culture technology.

Practice 3: Study of primary cell culture technique using chick embryo Practice 4: Animal cell batch culture technique.

Module-III (Transgenesis for livestock improvement-I): 4 H.

Theory: Overview of transgenic technology; Biopharming through animal transgenesis; Methods of producing transgenic farm animals; Identification and transfer of gene influencing better production and disease resistance.

Module-IV (Transgenesis for livestock improvement-II): 5 H.

Theory: Gene transfer methods in animals: Microinjection, Retrovirus mediated gene delivery, Embryonic stem cell mediated gene transfer; Knockout model systems & their utility; Animal as bioreactor.

Module-V (Assisted reproduction biotechnology for livestock improvement):4 H.

Theory: Reproduction biotechnologies and their use in livestock; Somatic cell nuclear transfer cloning; In Vitro Fertilization, Embryo production, preservation and transfer; Sperm and embryo sexing; Intracytoplasmic sperm injection (ICSI); Cryopreservation and gamete banking.

Module-VI (Animal Production technology for sustainable agriculture and food security): 7H.

Theory: Polyculture of fish for high yield; Edible oyster production; Pearl oyster production; Vermi-culture and vermicomposting for alternative and sustainable agriculture; Fish culture in flow through system and recirculation technology.

Practice 5 (2h): Vermicompost preparation from plant debris, cattle dung and paper waste.

Module-VII (Animal Biotechnology & human health): 6H.

Theory: Recombinant therapeutics and production of pharmaceuticals; Production of tissues and organs for humans and xenotransplantation; Process of gene therapy, Pros and cons in gene therapy; Retrovirus and adenovirus mediated gene therapy.

Practice 6(2h): Preparation of competent cell (Calcium chloride treatment method).

Text Books

  • Freshney RI (1992) Animal cell culture: a practical approach, Oxford University Press
  • Singh B, Gautam SK (2013) Text Book of Animal Biotechnology, TERI.

Reference Books

  • SinghB, Mal G, Gautam SK, Mukesh M (2019) Advances in Animal Biotechnology, Springer
  • Butler M (2003) Animal Cell Culture and Technology, Taylor & Francis

 

 

Session Plan

Session 1

Overview of animal cell and tissue culture technology.

PPT materials_Session 1

Session 2

Cell culture laboratory design and basic laboratory equipment.

PPT materials_Session 2

Session 3

Common laboratory hazards and Safety issues to consider in cell culture laboratory.

PPT Materials_Session 3

Laboratory Hazards and Safety Precautions

Session 4- Practice

Preparation of media for animal cell culture

PPT Materials_Session 4

Session 5- Practice

Sterilization techniques used in animal cell culture

Laboratory Hazards and Safety Precautions

Session 7

Characterization and preservation of animal cells

Book chapter_ACC characterization of cell line

Session 8

Challenges in animal cell and tissue culture technology

Challenges during animal cell culture

Session 9 - Practice

Primary culture preparation of chick embryo liver

PPT Materials_Chick embryo liver cell culture

Session 10

Animal cell batch culture technique

Batch Culture Technique

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