Basic Biochemistry CUTM 2604(3-2-0)


Venkata Kalyan Kankanala


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Course Name : Basic Biochemistry

Code(Credit) : CUTM2604(3-2-0)

Course Objectives

  • Biochemistry is the study of biological phenomena at the molecular level.
  • Its aim is to understand the fundamental chemical principles that govern complex biological systems.
  • The program is an interdepartmental major between biology and chemistry that emphasizes the importance of a solid foundation in the natural sciences.
  • The major focuses, however, on disciplines within biology and chemistry, ranging from cell biology and molecular biology to analytical chemistry and physical chemistry.

Learning Outcomes

  • Biochemistry helps in clinical diagnosis, understanding pathology of diseases, treatment of diseases, designing of drugs and understanding their metabolism and manufacture of various biological products like amino acids, proteins, antibiotics, hormones, enzymes, nutrients, etc.
  • It is expected that the students become conversant with the Fundamentals of Biochemistry which can be applied in clinical diagnosis of the metabolic disorders.

Course Syllabus


Enzymes - Introduction, definition, structure of enzyme, classification, coenzymes, isoenzymes, importance of enzyme inhibition


Chemistry of carbohydrates - Introduction, definition, classification, biomedical importance & properties

Brief outline of metabolism and significance of Glycogenesis, glycogenolysis, Gluconeogenesis, Glycolysis, citric acid cycle, HMP shunt



Amino acids - Definition, classification, essential & non-essential amino acids

Chemistry of Proteins - Introduction, definition, classification, biomedical importance


Ammonia formation & transport, Urea cycle, metabolic disorders in urea cycle

Importance substances derived from Phenylalanine, Tyrosine & Tryptophan, glycine



Chemistry of Lipids & their related metabolism - Introduction, definition, classification, biomedical importance, brief description about essential fatty acids

Fatty liver, Ketosis, Cholesterol & it's clinical significance, Lipoproteins and their importance



Blood glucose levels, HbA1C, glucose tolerance test, glycosuria,

Hyperglycemia & Hypoglycaemia & their causes




Diagnostic value and importance of Cardiac markers, LFT, RFT, Pancreatic markers, serum electrolytes, lipid profile, serum markers

Biochemistry practical

Quantitative exercises:

  • Detection of abnormal constituents in urine, sugar, proteins, ketones, blood and bile salts Bens Jones protein.
  • Phlebotomy equipment
  • Identification of Blood Collection Tubes & Centrifugal Separation of Blood Plasma and Serum Techniques:
  • Colorimeter, blood chemistry
  • Estimation of blood cholesterol
  • Estimation of alkaline Phosphate
  • Salivary amylase test (effect of PH and Temperature)
  • Estimation of Serum creatinine
  • Estimation of Serum uric Acid
  • Estimation of total proteins

Text boks

  1. Text book of Medical Laboratory Technology, B. Godkar 2nd Edn. 2003 Bhalani Publication.
  2. Text book of Biochemistry, A. Siddique 8th Edn.1993 Vijay Bhagat Scientific Book Co., Patna.
  3. Medical Biochemistry by AC
  4. Handbook of Christen Medical Association, India Medical Laboratory Technology- Robert Carman.

Session Plan

Session 1

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Session 2

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Venkata Kalyan Kankanala