Biochemistry deals with complete understanding of the molecular levels of the chemical process associated with living cells. The scope of the subject is providing biochemical facts and the principles to understand metabolism of nutrient molecules in physiological and pathological conditions. It is also emphasizing on genetic organization of mammalian genome and hetero & autocatalytic functions of DNA.
Upon completion of course student shell able to
1. Understand the catalytic role of enzymes, importance of enzyme inhibitors in design of new drugs, therapeutic and diagnostic applications of enzymes.
2. Understand the metabolism of nutrient molecules in physiological and
3. Understand the genetic organization of mammalian genome and functions of DNA in the synthesis of RNAs and proteins.
Qualitative analysis of carbohydrates (Glucose, Fructose, Lactose, Maltose, Sucrose and starch)
2. Identification tests for Proteins (albumin and Casein)
3. Quantitative analysis of reducing sugars (DNSA method) and Proteins (Biuret method)
4. Qualitative analysis of urine for abnormal constituents
5. Determination of blood creatinine
6. Determination of blood sugar
7. Determination of serum total cholesterol
8. Preparation of buffer solution and measurement of pH
9. Study of enzymatic hydrolysis of starch
10. Determination of Salivary amylase activity
11. Study the effect of Temperature on Salivary amylase activity.
12. Study the effect of substrate concentration on salivary amylase activity.