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• To study the historical development of bio process technology.
• To evaluate the kinetics and thermodynamics of enzymatic process
• To study the stoichiometry and energetics of cell growth and product formation
• To evaluate the kinetics and mechanism of microbial growth
This course enable the student to understand the practical aspects of bioprocess engineering and the role of bioprocess engineer in industry.
Module I: Title (4 hrs) Introduction to bioprocess engineering
Introduction to bioprocess engineering; Concept of Bioprocess; Role of bioprocess engineer in the biotechnology industry;
Practice:Culture media preparation (Solid)
Module II: (5 hrs ) Integrated bioprocess
Outline of an integrated bioprocess and the various (upstream and downstream) unit operations involved in bioprocesses; Generalized process flow sheets; A brief survey of organisms; processes, products and market economics relating to modern industrial biotechnology
Practice: Culture media preparation (Liquid broth)
Module III: Title (4 hrs) Kinetics of microbial growth
Phases of cell growth in batch cultures; Simple unstructured kinetic models for microbial growth; Monod model; Growth of filamentous organisms.
Practice: Bacterial growth curve
Module IV: Title (4 hrs) Microbial growth and product formation
Growth associated (primary) and non-growth associated (secondary) product formation kinetics, Leudking – Piret models, substrate and product inhibition on cell growth and product formation.
Practice: Study of growth kinetics
Module V: Title (4 hrs) Downstream processing
Introduction to processing; Removal of microbial cells and solid matter; Foam separation; precipitation; filtration, centrifugation, cell disruptions, liquid-liquid extraction.
Module VI: Title (4 hrs) Techniques used for downstream processing
Chromatography; Membrane process; Drying and crystallization; Effluent treatment: C.O.D treatment and disposal of effluents.
Module VII: Title (5 hrs) Industrial production of chemicals
Whole cell immobilization and their industrial applications; Industrial production of chemicals: Alcohol (ethanol), Acids (Citric, Acidic and Gluconic) solvents (glycerol, acetone, butanol) Antibiotics (penicillin, streptomycin, tetracycline) Aminoacids (lysine, glutamic acid), Single cell protein
Project: Growth kinetics of different bacteria available in our environment
Text Books:
1. Paulin M. Doran, Bioprocess Engineering Principles, Second Edition
Reference Books:
1. Michael L. Shuler, Fikret Kargi, Mathew Delisa, Bioprocess Engineering, Third Edition, Prentice Hall.
2. Pau Loke Show, Chien wei Ooi, Tau Chuan Ling, Bioprocess Engineering, CRC press, 1st Edition
Course outline Prepared by; Dr. Ranjan Kumar Sahoo Date:08-06-2020
Source of reference: NSQF
Introduction to Bioprocess Engineering
Introduction to Bioprocess Engineering PPT
Project: Growth kinetics of different bacteria isolated locally
Step 1: Isolation of bacteria from selected places of Centurion University.
Generalized process flow sheets of Bioprocess Engineering
Products and market economics relating to modern industrial biotechnology
Primary and Secondary growth of Microorganisms for product development
Leudking – Piret models
Project: Growth kinetics of different bacteria isolated locally
Step 2: Pure culture of isolated bacteria
Removal of microbial cells and solid matter from product
Centrifugation, cell disruptions and liquid-liquid extraction
Membrane process; Drying and crystallization
Effluent used in Chromatography technique, treatment of effluent
Components used in chromatography
Project: Growth kinetics of different bacteria isolated locally.
Step 3: Phenotypic identification of bacteria
Industrial production of chemicals: Alcohol (ethanol)
Project : Review of project work and analysis of report
Industrial production of chemicals: Acids (Citric, Acidic and Gluconic) solvents (glycerol, acetone, butanol)
Bacterial growth curve
Project: Growth kinetics of different bacteria available in our environment
Review and submission of report