Bioprocess Engineering

Teacher

Dr. Ranjan Kumar Sahoo

Category

Core Courses

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Course Name : Bioprocess Engineering

Code(Credit) : CUTM 1117(3-1-1)

COs Course outcomes Mapping COs with POs (High-3, Medium-2, Low-1)
CO1 Establishment a fundamental understanding of immunological processes at the cellular and molecular levels; describe key immunological ideas and concepts PO1 ( 3)
CO2 Describe, contrast, and show how the fundamental components of innate and adaptive immunity link in order to better understand the genetics underlying immunological diversity and the development of adaptive immune responses. PO1(3)
CO3 Identify the primary causes of inflammation and describe the essential processes and cellular agents that drive vaccination and the mechanisms of protection against infectious illnesses. PO3(3)
CO4  Utilization of The antigen- antibody interaction and different assays to understand interaction in diagnosis. PO4(3)
CO5 Utilize research-based knowledge and research methodologies, including experiment design, data analysis and interpretation, and information synthesis, to draw meaningful findings about the immune system. PO5(3)

Course Objectives

• To study the historical development of bio process technology.
• To evaluate the kinetics and thermodynamics of enzymatic process
• To study the stoichiometry and energetics of cell growth and product formation
• To evaluate the kinetics and mechanism of microbial growth

Learning Outcomes

This course enable the student to understand the practical aspects of bioprocess engineering and the role of bioprocess engineer in industry.

Course Syllabus

Module I: Title (4 hrs) Introduction to bioprocess engineering

Introduction to bioprocess engineering; Concept of Bioprocess; Role of bioprocess engineer in the biotechnology industry;

Practice:Culture media preparation (Solid)

Module II: (5 hrs ) Integrated bioprocess

Outline of an integrated bioprocess and the various (upstream and downstream) unit operations involved in bioprocesses; Generalized process flow sheets; A brief survey of organisms; processes, products and market economics relating to modern industrial biotechnology

Practice: Culture media preparation (Liquid broth)

Module III: Title (4 hrs) Kinetics of microbial growth

Phases of cell growth in batch cultures; Simple unstructured kinetic models for microbial growth; Monod model; Growth of filamentous organisms.

Practice: Bacterial growth curve

Module IV: Title (4 hrs) Microbial growth and product formation

Growth associated (primary) and non-growth associated (secondary) product formation kinetics, Leudking – Piret models, substrate and product inhibition on cell growth and product formation.

Practice: Study of growth kinetics

Module V: Title (4 hrs) Downstream processing

Introduction to processing; Removal of microbial cells and solid matter; Foam separation; precipitation; filtration, centrifugation, cell disruptions, liquid-liquid extraction.

Module VI: Title (4 hrs) Techniques used for downstream processing

Chromatography; Membrane process; Drying and crystallization; Effluent treatment: C.O.D treatment and disposal of effluents.

Module VII: Title (5 hrs) Industrial production of chemicals

Whole cell immobilization and their industrial applications; Industrial production of chemicals: Alcohol (ethanol), Acids (Citric, Acidic and Gluconic) solvents (glycerol, acetone, butanol) Antibiotics (penicillin, streptomycin, tetracycline) Aminoacids (lysine, glutamic acid), Single cell protein

Project: Growth kinetics of different bacteria available in our environment

Text Books:

1. Paulin M. Doran, Bioprocess Engineering Principles, Second Edition

Reference Books:

1. Michael L. Shuler, Fikret Kargi, Mathew Delisa, Bioprocess Engineering, Third Edition, Prentice Hall.
2. Pau Loke Show, Chien wei Ooi, Tau Chuan Ling, Bioprocess Engineering, CRC press, 1st Edition

Course outline Prepared by; Dr. Ranjan Kumar Sahoo Date:08-06-2020
Source of reference: NSQF

Session Plan

Session 1

Introduction to Bioprocess Engineering
Introduction to Bioprocess Engineering PPT

Session 2

Practice: Culture media preparation (solid)

 

Culture media preparation

Session 3

History of Bioprocess Engineering, Its scope and concept

 


Scope of Bioprocess Engineering

Session 4

Practice: Preparation of culture media (Liquid broth)

 

Liquid media preparation

Session 5

Role of bioprocess engineer in the biotechnology industry

 

Role of Bioprocess Engineer

Session 6

Generalized process flow of Fermenter


Session 6

Session 7

Project: Growth kinetics of different bacteria isolated locally

Step 1: Isolation of bacteria from selected places of Centurion University.

Session 8

Upstream and downstream processing

Upstream and downstream processing

Session 9

Generalized process flow sheets of Bioprocess Engineering

Session 10

Organisms involved in Bioprocess Engineering

Organisms involved in Bioprocessing

Session 11

Products and market economics relating to modern industrial biotechnology

Session 12

Useful Industrial products

Bioprocess products

Session 13

Growth of filamentous organisms

Filamentous bacteria

Session 14

Primary and Secondary growth of Microorganisms for product development

Session 15

Practice: Bacterial growth curve (Primary culture)

 

Practice: Growth curve

Session 16

Leudking – Piret models

Session 17

Substrate and product inhibition on cell growth

Feed back inhibition

Session 18

Project: Growth kinetics of different bacteria isolated locally
Step 2: Pure culture of isolated bacteria

Session 19

Removal of microbial cells and solid matter from product

Removal of microbial cells from products

Session 20

Steps in down stream processing, Foam separation; precipitation; filtration

Steps in downstream processing

Session 21

Centrifugation, cell disruptions and liquid-liquid extraction

Centrifugation in Bioprocess

Session 22

Introduction to chromatography, Types and its use

Chromatography

Session 23

Practice: Study of growth kinetics

Growth kinetics

Session 24

Membrane process; Drying and crystallization

Session 25

Effluent used in Chromatography technique, treatment of effluent
Components used in chromatography

Session 26

C.O.D treatment

C.O.D

Session 27

Project: Growth kinetics of different bacteria isolated locally.
Step 3: Phenotypic identification of bacteria

Session 28

Disposal of effluent
Disposal of effluent

Session 29

Introduction to immobilization, Whole cell immobilization

 

Immobilization

Session 30

Use of whole cell immobilization in Industry
Whole cell immobilization

Session 31

Industrial production of chemicals: Alcohol (ethanol)

Session 32

Project : Review of project work and analysis of report

Session 33

Industrial production of chemicals: Acids (Citric, Acidic and Gluconic) solvents (glycerol, acetone, butanol)

Industrial production

Session 34

Practice:Study of growth curve and growth kinetics


Practice: growth curve and kinetics

Session 35

Industrial production of Antibiotics (penicillin, streptomycin, tetracycline) Aminoacids (lysine, glutamic acid), Single cell protein
Industrial production


Industrial production

Session 36

Practice: Culture media preparation

Practice: Culture media preparation

Session 37

Practice: Culture media preparation (liquid)
Practice: Nutrient broth media

Session 38

Bacterial growth phase

Bacterial growth curve

Session 39

Bacterial growth curve

Session 40

Study of growth kinetics

Growth kinetics

Session 41

Project: Growth kinetics of different bacteria available in our environment

Review and submission of report

Case Studies

Case Studies

Our Main Teachers

Dr. Ranjan Kumar Sahoo

Associate Professor, Department of Biotechnology
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