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This course will cover in depth knowledge of Forensic Biotechnology in Pharmaceutical science, it’s disciplines and importance and working of FSL.
UNIT-I (Introduction to Recombinant DNA technology)
DNA modifying enzymes. Cloning strategies: Genomic libraries, cDNA libraries, single gene cloning. RAPD, RFLP and AFLP. Vectors: Types of vectors and choice of vectors- Plasmids, cosmids,lamda phage vectors, shuttle vectors, BACs and YACs. Transformation and Transfection. Expression systems in Eukaryotic cells, Yeast, Bacteria, Insect cell lines, Gene screening. Biosafety guidelines and containment strategies
UNIT-II (Bioprocess and Bio-chemical engineering)
Bioreactor design: Body construction, aeration and agitation, operation and applications. Microbial Growth: measurement, batch and continuous culture and its kinetics. Downstream processing: recovery and purification of products. Strains improvement. Fermentation economics.
UNIT-III (Animal and Plant biotechnology)
Cell lines: Definition, development, maintenance and management, established cell lines andtheir characteristic features. Transgenic animals- Creating transgenic animals, Example of transgenic animals-Dolly, Insects, Primates, mice. Somaclonal and gameto clonal variation: applications and limitations. Transgenic Plants: Herbicide eesistant, insect Resistant, drought/stress resistant, delayed ripening, Ti Plasmid and T-DNA transfer. Gene therapy: potential approach to gene therapy. Southern blotting, Northern blotting, Western blotting, Iso-electric focusing. Overview of Stem cells and its applications.
UNIT-IV (Biotherapeutics and industrial microbiology)
Introduction to industry important recombinant proteins, recombinant protein stability, Improvised recombinant protein secretion, Introduction to industrially important primary and secondary metabolites from plants and microbes. Microbial production: Antibiotics, acetic acid, lactic acid, wine, beer, SCP.
UNIT-V (Regulatory affairs and IPR)
Basic principles of quality control (QC) and quality assurance (QA). Guidelines for QA and QC: raw materials, products and validation. Introduction to pharmacopoeia. Intellectual Property Rights. Importance of protecting scientific discoveries. IPR policy of Government of India. Patent: Qualification (novel, commercial and non-obvious), jurisdiction of patent laws, Indian and international patent laws, filing procedures.
Reference Books:
DNA modifying enzymes
Cloning strategies: Genomic libraries
cDNA libraries, single gene cloning.
RAPD, RFLP and AFLP
Vectors: Types of vectors
choice of vectors- Plasmids, cosmids
lamda phage vectors, shuttle vectors
BACs and YACs
Transformation and Transfection
Expression systems in Eukaryotic cells
Biosafety guidelines and containment strategies.
Bioreactor design: Body construction
aeration and agitation, operation and applications
Microbial Growth: measurement
batch and continuous culture and its kinetics
Downstream processing
recovery and purification of products
Strains improvement
Fermentation economics
Cell lines: Definition, development, maintenance and management
established cell lines and their characteristic features.
Transgenic animals- Creating transgenic animals
Example of transgenic animals-Dolly, Insects, Primates, mice
Somaclonal and gameto clonal variation: applications and limitations.
Transgenic Plants: Herbicide eesistant, insect Resistant
drought/stress resistant, delayed ripening, Ti Plasmid and T-DNA transfer.
Gene therapy: potential approach to gene therapy
Southern Blotting
Northern Blotting
Western Blotting
Iso-electric Focusing
Overview of Stem cells and its applications
Introduction to industry important recombinant proteins, recombinant protein stability
Improvised recombinant protein secretion
Introduction to industrially important primary and secondary metabolites from plants and microbes
Microbial production: Antibiotics, acetic acid, lactic acid, wine, beer, SCP
Basic principles of quality control (QC) and quality assurance (QA)
Guidelines for QA and QC: raw materials, products and validation
Introduction to pharmacopoeia
Intellectual Property Rights
Importance of protecting scientific discoveries
IPR policy of Government of India
Patent: Qualification (novel, commercial and non-obvious), jurisdiction of patent laws, Indian and international patent laws, filing procedures.
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