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After completion of the course, the students will be able to
CO1: Describe the salient features of DNA structure, and the key concepts related to transcription, translation, and molecular diagnostics.
CO2: Explain the mechanism of DNA replication, as well as the concepts of RNA splicing, alternative splicing, polyadenylation, and capping.
CO3: Discuss the knowledge on the processes of malignant transformation of cells and the roles of oncogenes.
CO4: Apply their understanding of recombinant DNA technology, PCR, and gene therapy to real-world scenarios in molecular diagnostics.
CO5: Analyse the impact of mutations and alterations in DNA on disease development, evaluate the significance of molecular diagnostics techniques.
DNA structure. Salient features of double helix, Types of DNA. DNA topology - linking number, topoisomerases. Bidirectional and unidirectional replication, semi- conservative, semi- discontinuous replication.
Mechanism of DNA replication. Enzymes and proteins involved in DNA replication.
RNA Structure. Transcription: Promoter, Polymerase and the transcription unit. Transcription in Eukaryotes: RNA polymerases, general Transcription factors. Split genes, concept of introns and exons, RNA splicing, spliceosome machinery, concept of alternative splicing, Polyadenylation and capping, Processing of rRNA, RNA interference: si RNA, miRNA and its significance.
Translational machinery: Charging of tRNA, aminoacyl tRNA synthetases. Mechanisms of initiation, elongation and termination of polypeptides in both prokaryotes and eukaryotes.
Malignant transformation of cells and role of oncogenes, Tumor virus, Proto- oncogenes, Tumor suppressor genes; Apoptosis, cell regeneration.
Molecular diagnostics: Recombinant DNA Technology and its applications, Polymerase chain reaction and its application in diagnosis of pathogens, Site directed mutagenesis, DNA finger printing, DNase Foot Printing, antisense RNA technology, inherited genetic disorders in man and gene therapy.
(coursera link: https://www.coursera.org/learn/dna-decoded )
(Lecturio link: https://app.lecturio.com/#/course/s/8020 )
(Lecturio link: https://app.lecturio.com/#/lecture/s/5990/35832 )
All the practice components can be performed by simulation as well as virtual labs.
Amrita Vlab (https://www.youtube.com/channel/UC1ouqR_dIY_WPDPSZo0e8Wg).
Online Source: Labs for life, Lecturio, Coursera, article library, NCBI, Study.com, You tube, Frontier, Febs, unacademy, academia
Topic: DNA structure
Video on DNA structure
Slideshare link to DNA structure
Topic: Types of DNA
Video on Types of DNA
Slideshare link to Types of DNA
Topic: DNA topology - linking number, topoisomerases
Video on DNA topology - linking number, topoisomerases
Slideshare link to DNA topology
Topic: Semi- conservative, semi- discontinuous replication
Video on Semi- conservative, semi- discontinuous replication
Slideshare link to Semi- conservative replication
Topic: Mechanism of DNA replication
Video on Mechanism of DNA replication
Slideshare link to DNA replication
Topic: RNA Structure
Video on RNA Structure
Slideshare link to RNA Structure
Topic: Transcription in Eukaryotes
Video on Transcription in Eukaryotes
Slideshare link to Transcription in Eukaryotes
Topic: Split genes, concept of introns and exons
Video on Split genes, concept of introns and exons
Topic: RNA splicing, spliceosome machinery, concept of alternative splicing
Video on RNA splicing, spliceosome machinery, concept of alternative splicing
Slideshare link to RNA splicing
Topic: Polyadenylation and capping
Video on Polyadenylation and capping
Topic: Processing of rRNA
Video on Processing of rRNA
Topic: RNA interference: si RNA, miRNA and its significance
Video on RNA interference: si RNA, miRNA and its significance
Slideshare link to RNA interference
Topic: Translation: Charging of tRNA
Video on Translation: Charging of tRNA
Slideshare link to Translation
Topic: Aminoacyl tRNA synthetases
Video: Aminoacyl tRNA synthetases
Topic: Mechanisms of initiation, elongation and termination in Prokaryotes
Video on Mechanisms of initiation, elongation and termination in Prokaryotes
Slideshare link to Translation in Prokaryotes
Topic: Mechanisms of initiation, elongation and termination in Eukaryotes
Video on Mechanisms of initiation, elongation and termination in Eukaryotes
Slideshare link to Translation in Eukaryotes
Topic: Malignant transformation of cells and role of oncogenes
Video on Malignant transformation of cells and role of oncogenes
Slideshare link to Cancer development
Topic: Tumor virus
Video on Tumor virus
Slideshare link to Oncogenic Virus
Video on Proto-oncogenes
Slideshare link to Proto-oncogenes
Video on Apoptosis
Slideshare link to Apoptosis
Topic: Cell regeneration
Video on Cell regeneration
Slideshare link to Cell regeneration
Topic: Recombinant DNA Technology and its applications
Video on Recombinant DNA Technology and its applications
Slideshare link to Recombinant DNA Technology
Topic: Polymerase chain reaction and its application in diagnosis of pathogens
Video on Polymerase chain reaction and its application in diagnosis of pathogens
Slideshare link to Polymerase chain reaction
Topic: Site directed mutagenesis
Video on Site directed mutagenesis
Slideshare link to Site directed mutagenesis
Topic: DNA finger printing
Video on DNA finger printing
Slideshare link to DNA finger printing
Topic: DNase Foot Printing
Video on DNase Foot Printing
Topic: Antisense RNA technology
Video on Antisense RNA technology
Slideshare link to Antisense RNA technology
Topic: Inherited genetic disorders in man and gene therapy
Video on Inherited genetic disorders in man and gene therapy
Slideshare link to Gene Therapy
Dr. Soumya Jal is working at Centurion University of Technology and Management since 2018, prior to which she was associated with Rama Devi Women’s University as a visiting faculty. She has completed her Ph. D. in Medical Biotechnology from VIT University, Vellore (2016). Her research area includes fields such as, Applied and Clinical Microbiology, Molecular […]