Cell and Molecular Biology CUBS 2540(3-1-0)

Teacher

Venkata Kalyan Kankanala

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Course Name : Cell and Molecular Biology

Code(Credit) : CUBS 2540(3+1+0)

 

Course Objectives

  • Understanding the central dogma of life
  • To understand the concept of gene regulation and its impact
  • The use of several molecular diagnostic techniques for disease interpretation

Learning Outcomes

  • After completion of the course the student will be gain knowledge of the significance of genes and proteins.
  • They will understand the mechanism of gene expression and protein synthesis,
  • The significance of gene expression regulation will become clear.
  • The students will understand the use of several molecular techniques in disease diagnosis.

Course Syllabus

Module- 1:

What is living?; Biodiversity; Need for classification; Three domain of life; Taxonomy & Systematics; Concept of species and taxonomical hierarchy; Binomial nomenclature; Tools for study of Taxonomy–Museums, Zoos, Herbaria, Botanical gardens.Five kingdom classification; Salient features and classification of Monera; Protista and Fungi into major groups; Lichens; Viruses and Viroids.

Module- 2:

Biomolecules:Water, Amino Acids, Peptides, Proteins, Carbohydrates and their polymers, Nucleic acids, Nucleotides and Nucleosides, Fatty acids and lipids.

Module- 3:

Cell Morphology: Cell membrane, Cell organelles structure and function; Endomembrane system- endoplasmic reticulum, Golgi bodies, lysosomes, vacuoles; mitochondria, ribosomes, plastids, microbodies; Cytoskeleton, cilia, flagella, centrioles (ultra structure and function); Nucleus–nuclear membrane, chromatin, nucleolus., Types of cells.

Module- 4:

Cellular Genetics: DNA structure, Chromatin Structure, DNA Synthesis and repair, Transcription, Translation

Module- 5:

Cell Division: Growth of cells, Mitosis, Cancer.

 

Text Book:

  1. Waite, Gabi Nindle. Applied cell and molecular biology for engineers. McGraw-Hill Education, 2007.

Session Plan

Session 1

Introduction, classification, chemical nature and biological role of
carbohydrate

BIOMOLECULES Carbohydrate

 

https://www.youtube.com/watch?v=NlF1Kn6nVlE&t=641s

Session 2

Introduction, classification, chemical nature and biological role of lipids.

BIOMOLECULES lipid

 

https://www.youtube.com/watch?v=xZdTfhsypjM

Session 3

Introduction, classification, chemical nature and biological role of nucleic acids

BIOMOLECULES nuclic acid

https://www.youtube.com/watch?v=SV2dHT3Aoxw

Session 4

Introduction, classification, chemical nature and biological role of amino acids

BIOMOLECULES protein & amino acid

https://www.youtube.com/watch?v=N_n0iL3lY2A&t=651s

Session 5

Introduction, classification, chemical nature and biological role of proteins

BIOMOLECULES protein

https://www.youtube.com/watch?v=FrrqNLJCSUg&t=477s

Session 6

Topic: Structure of enzyme: Apoenzyme and cofactors, prosthetic group-TPP, coenzyme NAD, metal cofactors

Video on Introduction on Enzymes

https://app.lecturio.com/#/lecture/s/8184/36358

https://www.youtube.com/watch?v=qgVFkRn8f10

https://www.youtube.com/watch?v=yk14dOOvwMk

Slideshare link to Introduction to Enzymes

https://www.slideshare.net/RichardBader/enzymes-14106495

https://www.slideshare.net/Ramzanken/enzymes-biochemistry

Session 7

Topic: Classification of enzymes

Video on Classification of enzymes

https://www.youtube.com/watch?v=0Nr9Pc00qO

Slideshare link to Classification of enzymes

https://www.slideshare.net/rakhiadarsh/classification-of-enzymes

https://www.slideshare.net/ArunViswanathan3/enzyme-199841983

Session 8

Topic: Mechanism of action of enzymes: active site, transition state complex and activation energy

Video on Mechanism of action of enzymes

https://app.lecturio.com/#/course/s/8242

https://www.youtube.com/watch?v=bTvG0UTJt9E

https://www.youtube.com/watch?v=ie_7Y7HJlps

https://www.youtube.com/watch?v=1BoZfsARSjk

Slideshare link on Mechanism of action of enzymes

https://www.slideshare.net/karthi131087/final-44690151

Session 9

Topic: Lock and key hypothesis, and Induced Fit hypothesis

Video on Lock and key hypothesis, and Induced Fit hypothesis

https://www.youtube.com/watch?v=MHN9FLuJtXY

https://www.youtube.com/watch?v=hXeMb-CBTW0

https://www.youtube.com/watch?v=EiMBsgNZh-M

https://www.youtube.com/watch?v=V8TOWTp71tE

https://www.youtube.com/watch?v=pVoytz_3H_s

Slideshare link to Lock and key hypothesis, and Induced Fit hypothesis

https://www.slideshare.net/DeepakKumarGupta2/enzyme-50519282

 

Session 10

Session 11

Session 12

Topic -Defination and History of Cell
Refer Book - Cell Biology (7th Edition ) by Karp G, John Wiley Page no. 02
The Cell a Molecular Approach (4th Edition ) by Cooper & Hausman. Page no.4-15
Video - https://youtu.be/4OpBylwH9DU
https://youtu.be/LFyjJBiltFI

Presentation - https://www.slideshare.net/RaihaBilal/introduction-cell
https://www.slideshare.net/earshadshinichi/cell-biology-the-cell-its-structure-and-history

Session 14

Topic - Structure and Function of Cell and its Organelles
Refer Book - The Cell a Molecular Approach (4th Edition ) by Cooper & Hausman. Page no.353
Video - https://youtu.be/V6s0xOTNmT4
https://youtu.be/Hmwvj9X4GNY
Presentation - https://www.slideshare.net/pugazhkurianc/cell-structure-and-function-10144056
https://www.slideshare.net/wthompsonctems/keynote-ch-3-cell-structure-function

Session 15

Topic - Biological membranes
Refer Book -The Cell a Molecular Approach (4th Edition ) by Cooper & Hausman. Page no 58
Video - https://youtu.be/uhZ_ALt51TM
Presentation - https://www.slideshare.net/PradeepNarwat/biological-membrane-its-chemical-composition

Session 16

Topic - Nucleus
Refer Book - The Cell a Molecular Approach (4th Edition ) by Cooper & Hausman. Page no 355
Video - https://youtu.be/gGeC0ntvxR8
https://youtu.be/EmlJOvDawXI
Presentation - https://www.slideshare.net/saritananda/nucleus-81376601
https://www.slideshare.net/akshaygmore27/nucleus-54263255

Session 17

Topic - Nuclear envelope
Refer Book - The Cell a Molecular Approach (4th Edition) by Cooper & Hausman. Page no 356
Video - https://youtu.be/tQyeSpLlSGg
https://youtu.be/Vkz-JZVXq7o

Session 18

Topic - Nucleolus
Refer Book - The Cell a Molecular Approach (4th Edition) by Cooper & Hausman. Page no 375
Video - https://youtu.be/HobSglXNtYk
https://youtu.be/kAKPiVb7nYU

Session 19

Topic - Mitochondria
Refer Book - Cell Biology (7th Edition ) by Karp G, John Wiley page no. 179
The Cell a Molecular Approach (4th Edition) by Cooper & Hausman. Page no .434
Video - https://youtu.be/CVs4WLdQDco
https://youtu.be/39HTpUG1MwQ
Presentation - https://www.slideshare.net/devadevi666/mitochondria-ppt

Session 20

Topic - Chloroplasts
Refer Book - Cell Biology (7th Edition ) by Karp G, John Wiley page no- 213
The Cell a Molecular Approach (4th Edition) by Cooper & Hausman. Page no .451
Video - https://youtu.be/uewfzOH-Vu4
Presentation - https://www.slideshare.net/kaushal2683/chloroplast-by-kk-sahu-sir

Session 21

Topic - Lysosomes
Refer Book - Cell Biology (7th Edition ) by Karp G, John Wiley page no- 303
The Cell a Molecular Approach (4th Edition) by Cooper & Hausman. Page no.424
Video - https://youtu.be/TDRtEszzs78
https://youtu.be/02jrzh9m92M
Presentation - https://www.slideshare.net/squid923/lysosome-powerpoint

Session 22

Topic - Gloxysomes and Peroxisomes
Refer Book - Cell Biology (7th Edition ) by Karp G, John Wiley page no- 206
The Cell a Molecular Approach (4th Edition) by Cooper & Hausman. Page no 462
Video - https://www.slideshare.net/Dilippandya/peroxisome-46636423

Session 23

Topic - Endoplasmic reticulum
Refer Book - The Cell a Molecular Approach (4th Edition) by Cooper & Hausman. Page no.386
Video - https://youtu.be/QO4WwyVt3cU
Presentation - https://www.slideshare.net/AshishNain/endoplasmic-reticulum-cell-organelle

Session 24

Topic - Golgi complex (Structural organization function, marker enzymes of the above organelles)

Refer Book - The Cell a Molecular Approach (4th Edition) by Cooper & Hausman. Page no. 408

Video - https://youtu.be/CfnH1BT-NjE
Presentation - https://www.slideshare.net/tukaramgitte/golgi-complex-160381391

Session 25

Topic - Cell types: prokaryotes vs. eukaryotes
Refer Book - The Cell a Molecular Approach (4th Edition) by Cooper & Hausman. Page no.529
Video - https://youtu.be/xTnNv7YplSo
Presentation - https://www.slideshare.net/CandelaContent/lab-2-microbiology
https://www.slideshare.net/gkumarimahesh/prokaryotic-and-eukaryotic-algal-cell-structure

Topic - From single cell to multi-cellular organism
Video - https://youtu.be/-BobUiQRhJ0

Session 26

Topic - Cell cycle and its regulation
Refer Book - The Cell a Molecular Approach (4th Edition) by Cooper & Hausman. Page no.599
Video - https://youtu.be/gTZ_vj-HdzM
Presentation - https://www.slideshare.net/abishadh/cell-cycle-in-relation-to-cancer

Session 27

Topic - Cellular communication and cell mobility
Video - https://youtu.be/QYv_n7tGfro

Session 28

Topic - Cell cycle: G0/G1, S, G2 and M phages
Refer Book - The Cell a Molecular Approach (4th Edition) by Cooper & Hausman. Page no. 649
Video - https://youtu.be/PQhQ80UGwBo
Presentation - https://www.slideshare.net/abishadh/cell-cycle-in-relation-to-cancer

Session 29

Topic - Cell Division: Mitosis, meiosis and cytokinesis
Refer Book - The Cell a Molecular Approach (4th Edition) by Cooper & Hausman. Page no. 649
Video - https://youtu.be/6ojLPvt90aI
https://youtu.be/QbdFcQSF_tY

Session 30

Topic - Regulation of cell cycle
Video - https://youtu.be/hSTeawTvoIU

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Session 2

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Venkata Kalyan Kankanala