Course Code : CUTM2312 (3-2-0)
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Course Code : CUTM2312 (3-2-0)
To learn about
After successful completion of the course, the students will be able to :
Unit I: Microscopy I
Fundamental & principles and Applications of Microscopes, Different types of Microscopes-Simple
Microscope, Compound Microscope, Stereo Microscope, Comparison Microscope
Unit II: Microscopy II
Principles of Microscopes – Phase contrast, Polarizing, Fluorescent microscope, IR microscope, SEM
and TEM
Unit III: Spectroscopic Techniques I
Basic Fundamentals, Different types of Spectroscopy-EMR, UV-VIS, IR, Fourier-transform and
Fourier-transform infrared Spectroscopy atomic absorption Spectroscopy, Atomic Emission
Spectroscopy - Principles and Forensic Application
Unit IV: Spectroscopic Techniques II
Basic Fundamentals of Raman spectroscopy, Photo-thermal spectroscopy, XRD, XRF, NMR, Mass
Spectroscopy – Principles & Forensic Application
Unit V: Chromatographic Techniques I
Introduction of Chromatography, Basics of separation of molecules, Types of chromatography,
Working principles of Paper chromatography, Thin Layer Chromatography, Column chromatography.
Unit VI: Chromatographic Techniques II
Principles and Applications of chromatographic techniques - Ion exchange chromatography, Gas
Chromatography, Gas Liquid Chromatography, High Performance Liquid Chromatography, High
Performance Thin Layer Chromatography.
Unit VII: Centrifugation and Electrophoresis
Basic fundamentals of molecular separation methodologies and parts of centrifuge – Bench top
centrifugation, micro centrifugation, Low speed centrifugation, Ultra centrifugation, Gas
centrifugation.
Fundamentals of electrophoresis – Agarose gel electrophoresis, Poly acrylamide Gel electrophoresis.
Session 1
Microscopy I
https://cattheni.edu.in/wp-content/uploads/2018/09/2.Microscopy-Principles-and-Types.pdf
Session 2
Microscopy II
https://cattheni.edu.in/wp-content/uploads/2018/09/2.Microscopy-Principles-and-Types.pdf
Session 3
UV-VIS Spectroscopy
https://www.youtube.com/watch?v=5yLxJ5Vn5gw&list=PLQnNyE1lxfVLaV7GIpKYfGYiHsZiTYB5u&index=2
Session 4
IR Spectroscopy
https://www.youtube.com/watch?v=4P8JZP-c-48&list=PLQnNyE1lxfVLaV7GIpKYfGYiHsZiTYB5u&index=9
Session 5
Atomic Absorption Spectroscopy
https://www.youtube.com/watch?v=a8cwwLeezmo&list=PLQnNyE1lxfVLaV7GIpKYfGYiHsZiTYB5u&index=13
Session 6
Introduction to chromatography
https://www.youtube.com/watch?v=VUFhLCt2cCg&list=PLQnNyE1lxfVLaV7GIpKYfGYiHsZiTYB5u&index=15
Session 7
Column Chromatography
https://www.youtube.com/watch?v=w0y1RLrcBE8&list=PLQnNyE1lxfVLaV7GIpKYfGYiHsZiTYB5u&index=16
Session 8
Principles of Different types of Chromatography
https://ncert.nic.in/pdf/publication/sciencelaboratorymanuals/classXII/chemistry/lelm105.pdf
Session 13
Fundamentals of Electrophoresis
http://courseware.cutm.ac.in/wp-content/uploads/2023/04/Lecture-ITechniquesinBiochemistry_2020.pdf
Assistant Professor, Department of Forensic Science, School of Paramedical and Allied Health Sciences, Centurion University of Technology and Management, Vizianagaram