V. Pravallika


Core Courses

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Course Code : CUTM2312 (3-2-0)

Course Objective

To learn about

  • Basic concepts of chromatography and different chromatographic techniques.
  • Principle of different microscopes and their forensic significance.
  • Concepts of different spectroscopic techniques and their forensic application.
  • Basics of electrophoretic techniques and centrifugation methods.

Course Outcome

After successful completion of the course, the students will be able to :

  • Perform TLC, paper chromatography and demonstrate sophisticated chromatographic techniques.
  • Demonstrate the working principle of different microscopes and apply them in identification of various forensic evidences.
  • Gain knowledge about the instrumentation of spectroscopic techniques and their application in forensic science.
  • Gain skill in performing centrifugation and agarose gel electrophoresis.


Unit I: Microscopy I

Fundamental & principles and Applications of Microscopes, Different types of Microscopes-Simple
Microscope, Compound Microscope, Stereo Microscope, Comparison Microscope

Unit II: Microscopy II

Principles of Microscopes – Phase contrast, Polarizing, Fluorescent microscope, IR microscope, SEM
and TEM

Unit III: Spectroscopic Techniques I

Basic Fundamentals, Different types of Spectroscopy-EMR, UV-VIS, IR, Fourier-transform and
Fourier-transform infrared Spectroscopy atomic absorption Spectroscopy, Atomic Emission
Spectroscopy - Principles and Forensic Application

Unit IV: Spectroscopic Techniques II

Basic Fundamentals of Raman spectroscopy, Photo-thermal spectroscopy, XRD, XRF, NMR, Mass
Spectroscopy – Principles & Forensic Application

Unit V: Chromatographic Techniques I

Introduction of Chromatography, Basics of separation of molecules, Types of chromatography,
Working principles of Paper chromatography, Thin Layer Chromatography, Column chromatography.

Unit VI: Chromatographic Techniques II

Principles and Applications of chromatographic techniques - Ion exchange chromatography, Gas
Chromatography, Gas Liquid Chromatography, High Performance Liquid Chromatography, High
Performance Thin Layer Chromatography.

Unit VII: Centrifugation and Electrophoresis

Basic fundamentals of molecular separation methodologies and parts of centrifuge – Bench top
centrifugation, micro centrifugation, Low speed centrifugation, Ultra centrifugation, Gas
Fundamentals of electrophoresis – Agarose gel electrophoresis, Poly acrylamide Gel electrophoresis.

Session 9

High Performance Liquid Chromatography

Session 10

Gas Chromatography and Mass Spectroscopy

Session 11

High Performance Thin Layer Chromatography

Suggested Reading

  1. Barbara Stuart, Forensic Analytical Techniques
  3. Dr. G.R. Chatwal, Instrumental Methods of Chemical Analysis
  4. Books LLC, Wiki Series, Laboratory Techniques: Polymerase Chain Reaction, Gel Electrophoresis, Distillation, Microscopy, Immunoperoxidase
  5. Imran Latif Patel, Biochemical and Biophysical Techniques


  1. To determine the concentration of a coloured compound by calorimetry analysis.
  2. To carry out thin layer chromatography of ink samples.
  3. To carry out separation of organic compounds by paper chromatography.
  4. To identify drug samples using UV-Visible spectroscopy.
  5. To perform Agarose Gel Electrophoresis by using any forensic sample
  6. To Separate the Molecules by using Ultra centrifugation
  7. To identify the Blood cells by using Microscope.
  8. To perform the protein analysis by using Poly Acrylamide Gel Electrophoresis.
  9. To determine the chlorophyll by using UV-Visible spectroscopy.
  10. To determine the caffeine and benzoic acid in soft drinks by using FT-IR.

Our Main Teachers

V. Pravallika

Assistant Professor

Assistant Professor, Department of Forensic Science, School of Paramedical and Allied Health Sciences, Centurion University of Technology and Management, Vizianagaram