Diagnostic Mycology


Dr. Monali Priyadarsini Mishra


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Course Name : CUTM1745-Diagnostic Mycology

Code(Credit) : (3-1-0)

Course Objectives

  • To confirm the suspicion of fungal disease.
  • To identify the etiologic agent by isolating the causative fungal pathogen.

Learning Outcomes

  • This course provides learning opportunities in the basic principles of medical microbiology and infectious fungal diseases.
  • The course provides the conceptual basis for understanding pathogenic fungi and the mechanisms by which they cause disease in the human body.
  • Student can safeguard himself & society and can work diagnostics and hospitals 

Course Syllabus


Diagnostic Mycology: Medically important fungi, Opportunistic Fungi

Fungal Diseases: Mycoses

  1. Superficial mycoses
  2. Subcutaneous mycoses
  3. Cutaneous mycoses: Trichophytosis, Microsporiosis, Epidermophytosis.
  4. Systemic mycoses: Histoplamosis, Blastomycosis, Cryptococcosis, Coccidioidosis, Paracoccidioidosis .


  1. Antifungal Sensitivity Test: Antibiotics.
  2. Preparation of stock solution of drug

Module- 2 

Pathogenesis and Laboratory Diagnosis of Mycotic Infections: Aspergillus spp, Candida albicans, Fusarium spp, Cryptococcus neoformans, Histroplasma capsulatum, Sporothourixspp, Philophora spp., Trichophotonmicrosporium, Epidermphyton spp. Blastomyces dermastitidis, Coccidiodisimmitens.


  1. Dilution technique
  2. Determination of Minimal inhibitory concentration (MIC)

Unit 3

Isolation and Identification of Fungi (Laboratory Diagnosis):A. Selection, collection and transportation of specimens 5 hours Skin, Hair, Nail, Mucous membranes, Ear, eye, Cornial ulcer, Pus, Blood, Biopsy, Sputum, Urine, Vaginal and Cervical swab, Stool samples, Plural and peritonial fluid, Superficial, sub-cutanious and cutanious samples.B. Smear Preparation: 2 hours KOH Preparation, 20% KOH with 20% Glycerol, KOH – DMSO (DimethyalSulphoxide) 100% Lactophenol Cotton Blue, India ink preparation.


  1. Preparation of different media, chemical and stain for fungus study
  2. Isolation and identification of different fungi of medical important

Suggested References:

  1. Textbook of Microbiology- Ananthanarayan & Paniker (10th Ed)
  2. Medical Microbiology-by Fritz H. Kayser et al
  3. Fundamental medical mycology / Errol Reiss, H. Jean Shadomy, and G. Marshall Lyon III
  4. Essential Medical Microbiology- by Rajesh Bhatia (4th Ed)
  5. Clinical Microbiology Procedures Handbook- by Amy L. Leber (4th Ed)
  6. The short text book of medical microbiology- by Satis Gupte (10th Ed)

Session Plan

Session 1

Introduction to Diagnostic Mycology: Medically important fungi

Video: https://www.youtube.com/watch?v=f7QxqWEKoGs

Presentatuion: https://www.slideshare.net/mgetso/introduction-to-medical-mycology

Session 2

Session 3

Session 4

Session 5

Session 6

Session 7

Session 8

Session 9

Session 10

Session 11


Antifungal Sensitivity Test: Antibiotics.

Video: https://www.youtube.com/watch?v=coTMjdvuTY8

Video: https://www.youtube.com/watch?v=3Rw11wPbw4I


Preparation of stock solution of drug

Video: https://www.youtube.com/watch?v=5RQbMDfc2BM

Session 12

Pathogenesis and Laboratory Diagnosis of Mycotic Infections: Aspergillus spp

Video: https://www.youtube.com/watch?v=N1UUazCiL78

Video: https://www.youtube.com/watch?v=NDrbHSIzcLI

Presentation: https://www.slideshare.net/rajhaviknesh/aspergillosis-eassignm

Session 13

Session 14

Session 15

Session 16

Session 17

Session 18

Session 19

Session 20

Session 21

Session 22


Dilution technique



Determination of Minimal inhibitory concentration (MIC)



Session 23

Session 24

Selection, collection and transportation of specimens from  Nail

Video: https://www.youtube.com/watch?v=WyyjhA5gG7M

Presentation: https://www.slideshare.net/albertgonzalobautista/2-specimen-collection

Session 25

Session 26

Session 27

Session 28

Session 29

Session 30

Session 31

Session 32

Session 33

Session 34

Session 35


Preparation of different media, chemical and stain for fungus study




Isolation and identification of different fungi of medical important


Case Studies

Case Studies

Our Main Teachers

Dr. Monali Priyadarsini Mishra is currently working as Assistant Professor in CUTM, Bhubaneswar. Her area of research belongs to “Clinical and Applied Microbiology, Biotechnology, Phytochemistry”. She has completed her Ph.D. from Siksha ‘O’ Anusandhan University, Bhubaneswar. She has authored several scientific papers in peer-reviewed international journals. Currently, She is working on some biologically active antimicrobial […]