Diagnostic Mycology
Teacher
Dr. Monali Priyadarsini Mishra
Category
Core Courses
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Course Name : CUTM1745-Diagnostic Mycology
Code(Credit) : (3-1-0)
Course Objectives
- To confirm the suspicion of fungal disease.
- To identify the etiologic agent by isolating the causative fungal pathogen.
Learning Outcomes
- This course provides learning opportunities in the basic principles of medical microbiology and infectious fungal diseases.
- The course provides the conceptual basis for understanding pathogenic fungi and the mechanisms by which they cause disease in the human body.
- Student can safeguard himself & society and can work diagnostics and hospitals
Course Syllabus
Module-1
Diagnostic Mycology: Medically important fungi, Opportunistic Fungi
Fungal Diseases: Mycoses
- Superficial mycoses
- Subcutaneous mycoses
- Cutaneous mycoses: Trichophytosis, Microsporiosis, Epidermophytosis.
- Systemic mycoses: Histoplamosis, Blastomycosis, Cryptococcosis, Coccidioidosis, Paracoccidioidosis .
Practice:
- Antifungal Sensitivity Test: Antibiotics.
- Preparation of stock solution of drug
Module- 2
Pathogenesis and Laboratory Diagnosis of Mycotic Infections: Aspergillus spp, Candida albicans, Fusarium spp, Cryptococcus neoformans, Histroplasma capsulatum, Sporothourixspp, Philophora spp., Trichophotonmicrosporium, Epidermphyton spp. Blastomyces dermastitidis, Coccidiodisimmitens.
Practice:
- Dilution technique
- Determination of Minimal inhibitory concentration (MIC)
Unit 3
Isolation and Identification of Fungi (Laboratory Diagnosis):A. Selection, collection and transportation of specimens 5 hours Skin, Hair, Nail, Mucous membranes, Ear, eye, Cornial ulcer, Pus, Blood, Biopsy, Sputum, Urine, Vaginal and Cervical swab, Stool samples, Plural and peritonial fluid, Superficial, sub-cutanious and cutanious samples.B. Smear Preparation: 2 hours KOH Preparation, 20% KOH with 20% Glycerol, KOH – DMSO (DimethyalSulphoxide) 100% Lactophenol Cotton Blue, India ink preparation.
Practice:
- Preparation of different media, chemical and stain for fungus study
- Isolation and identification of different fungi of medical important
Suggested References:
- Textbook of Microbiology- Ananthanarayan & Paniker (10th Ed)
- Medical Microbiology-by Fritz H. Kayser et al
- Fundamental medical mycology / Errol Reiss, H. Jean Shadomy, and G. Marshall Lyon III
- Essential Medical Microbiology- by Rajesh Bhatia (4th Ed)
- Clinical Microbiology Procedures Handbook- by Amy L. Leber (4th Ed)
- The short text book of medical microbiology- by Satis Gupte (10th Ed)
Session Plan
Session 1
Introduction to Diagnostic Mycology: Medically important fungi
Video: https://www.youtube.com/watch?v=f7QxqWEKoGs
Presentatuion: https://www.slideshare.net/mgetso/introduction-to-medical-mycology
Session 2
Opportunistic Fungi
Video: https://www.youtube.com/watch?v=L6sZUk0kVtY
Presentation: https://www.slideshare.net/doctorrao/opportunistic-fungal-infections
Session 3
Fungal Diseases: Mycoses
Video: https://www.youtube.com/watch?v=RoDKdgk58SE
Presentation: https://www.slideshare.net/majid4uonly/mycoses
Session 4
Superficial mycoses
Video: https://www.youtube.com/watch?v=GV840R4Pyi4&t=353s
Presentation: https://www.slideshare.net/MizanRahman9/superficial-mycoses-63214580
Session 5
Subcutaneous mycoses
Video: https://www.youtube.com/watch?v=gvcDExVxWv0
Presentation: https://www.slideshare.net/RakeshPrasadSah/classification-of-mycoses-by-dr-rakesh-prasad-sah-116628023
Session 6
Cutaneous mycoses: Trichophytosis
Video: https://www.youtube.com/watch?v=GdgRPj7zmwA
Presentation: https://www.slideshare.net/PrincessAlenAguilar/trichophyton-and-epidermophyton
Session 7
Microsporiosis, Epidermophytosis
Video :https://www.youtube.com/watch?v=u4EYsWM5I44
Video :https://www.youtube.com/watch?v=9Nzt8cc66LU
Presentation: https://www.slideshare.net/rubaiyakabir/dermatophytes-69013431
Session 8
Systemic mycoses: Histoplasmosis
Video: https://www.youtube.com/watch?v=bLpxM_8i3SA
Presentation: https://www.slideshare.net/thilgar/systemic-mycosis-36158507
Session 9
Blastomycosis
Video: https://www.youtube.com/watch?v=bLpxM_8i3SA
https://www.youtube.com/watch?v=YWgfVL172Es
Presentation: https://www.slideshare.net/thilgar/systemic-mycosis-36158507
Session 10
Cryptococcosis
Video: https://www.youtube.com/watch?v=hqefanNgOY8
Presentation: https://www.slideshare.net/MaryMwinga/cryptococcosis-97889849
Session 11
Coccidioidosis, Paracoccidioidosis
Video: https://www.youtube.com/watch?v=bLpxM_8i3SA
https://www.youtube.com/watch?v=a231-qmICZM
Presentation: https://www.slideshare.net/thilgar/systemic-mycosis-36158507
Practice:
Antifungal Sensitivity Test: Antibiotics.
Session 12
Pathogenesis and Laboratory Diagnosis of Mycotic Infections: Aspergillus spp
Video: https://www.youtube.com/watch?v=N1UUazCiL78
Video: https://www.youtube.com/watch?v=NDrbHSIzcLI
Presentation: https://www.slideshare.net/rajhaviknesh/aspergillosis-eassignm
Session 13
Candida albicans
Video: https://www.youtube.com/watch?v=OSlFOSLPFlM
Video: https://www.youtube.com/watch?v=4G1nFqPPRAA
Prfesentation: https://www.slideshare.net/mafambakhathu/candidiasis-46179900
Session 14
Session 15
Cryptococcus neoformans
Video: https://www.youtube.com/watch?v=7SL-0J_zCT8
Video: https://www.youtube.com/watch?v=Y8bd8TPFO0A
Presentation: https://www.slideshare.net/doctorrao/cryptococcus-neoformans-9872069
Session 16
Histroplasma capsulatum
Video: https://www.youtube.com/watch?v=T8T9Sk8B1_g
Presentation: https://www.slideshare.net/shahmanthan24/histoplasmois-mycology-epidemiology-laboratory-diagnosis
Session 17
Histroplasma capsulatum
Video: https://www.youtube.com/watch?v=T8T9Sk8B1_g
Presentation: https://www.slideshare.net/shahmanthan24/histoplasmois-mycology-epidemiology-laboratory-diagnosis
Session 18
Sporothrix spp
Video: https://www.youtube.com/watch?v=RjwpO6yLgVQ
Presentation: https://www.slideshare.net/YuvrajPanth1/sporotrichosis-120705098
Session 19
Session 20
Epidermphyton spp
Video: https://www.youtube.com/watch?v=Kh4eS1CX6gA
Presentation: https://www.slideshare.net/PrincessAlenAguilar/trichophyton-and-epidermophyton
Session 21
Blastomyces dermatitidis
Video: https://www.youtube.com/watch?v=eMRsqejBZWo
Presentation: https://cmr.asm.org/content/23/2/367
Session 22
Coccidiodes immitis
Practice:
Determination of Minimal inhibitory concentration (MIC)
Session 23
Isolation and Identification of Fungi
Video: https://www.youtube.com/watch?v=TCStKcxoJc8
Presentation: https://www.slideshare.net/MrSSenthilPrabhu/identification-of-fungi
Session 24
Selection, collection and transportation of specimens from Nail
Video: https://www.youtube.com/watch?v=WyyjhA5gG7M
Presentation: https://www.slideshare.net/albertgonzalobautista/2-specimen-collection
Session 25
Session 26
Session 27
Corneal ulcer, Pus, Blood
Video: https://www.youtube.com/watch?v=TdTS24YtI1c
https://www.youtube.com/watch?v=SHx_P08m3_Y
Source: https://www.palomarhealth.org/media/file/Lab/SpecimenCollectionHandling%20Manual.pdf
Session 28
Session 29
Urine, Vaginal and Cervical swab
Video: https://www.youtube.com/watch?v=T9eOPhpUeF8
https://www.youtube.com/watch?v=FxudfPMN5wI
Source: https://www.palomarhealth.org/media/file/Lab/SpecimenCollectionHandling%20Manual.pdf
Session 30
Session 31
Plural and peritonial fluid
https://www.palomarhealth.org/media/file/Lab/SpecimenCollectionHandling%20Manual.pdf
Session 32
Superficial, subcutaneous and cutaneous samples
https://www.palomarhealth.org/media/file/Lab/SpecimenCollectionHandling%20Manual.pdf
Session 33
Smear Preparation: 2 hours KOH Preparation, 20% KOH with 20% Glycerol
Video: https://www.youtube.com/watch?v=6KdmTjakl_s
https://www.youtube.com/watch?v=GyZrKXPeoVw
Source: https://www.dalynn.com/dyn/ck_assets/files/tech/RP85-25.pdf
https://catalog.hardydiagnostics.com/cp_prod/Content/hugo/PotassiumHydroxideSolns.htm
Session 34
Lactophenol Cotton Blue Mount
Session 35
India ink preparation
Video: https://www.youtube.com/watch?v=HwC7piON9hY
Source: https://www.medindia.net/bloodtest/microbiology/india_ink_preparation.htm
Practice:
Preparation of different media, chemical and stain for fungus study
https://www.youtube.com/watch?v=FcW5tHrbAos
Practice:
Isolation and identification of different fungi of medical important
Case Studies
Case Studies
Our Main Teachers
Dr. Monali Priyadarsini Mishra is currently working as Assistant Professor in CUTM, Bhubaneswar. Her area of research belongs to “Clinical and Applied Microbiology, Biotechnology, Phytochemistry”. She has completed her Ph.D. from Siksha ‘O’ Anusandhan University, Bhubaneswar. She has authored several scientific papers in peer-reviewed international journals. Currently, She is working on some biologically active antimicrobial […]