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Introduction, brief history to immunology. Types of immunity: Innate and adaptive immunity, cell mediated and humoral immunity, cells and organs of the immune system. Antigens – structure and types. epitopes, haptenes.Antibody – fine structure, classes with structure and functions, antigenic determinants on immunoglobulins.MHC complex – types, structure, and functions.Antigen-antibody interactions- principle, antigenrecognition by B-cells and T cells.Antigen-antibody reaction - Precipittin reactions, agglutination reactions, Microorganisms associated with fishes in health and disease.Defense mechanism in finfish and shellfish- specific and non specific immune system.Pathogenicity and virulence.Sources of infection, transmission of disease producing organisms, portals of infection.Immunity to bacteria, fungi and parasites Role of stress and host defense mechanism in disease development.Vaccines - types of vaccines – whole cell vaccine, purified macromolecules, recombinant –vector, DNA vaccines and multivalent subunit vaccines, modes of vaccine administration.Serological methods in disease diagnosis.Immunostimulants –types, mechanism of action, modes of administration. Immunoassays, immunodiffusion, ELISA, immunofluorescence, neutralization, radioimmunoassay, serotyping
1. Collection, separation and identification of fish leucocytes.
2. Separation of blood plasma and serum.
3. Differential counting - RBC and WBC by Haemocytometer.
4. Study of different types of leukocytes and isolation of macrophages.
5. Precipitin reactions - Agglutination test,immunogel diffusion, double immuno diffusion, radial immuno diffusion assay, ELISA.
6. Methods of vaccine preparation and techniques of fish immunization.
General concepts in immunology
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-I.pdf
YouTube Link https://www.youtube.com/watch?v=wHCJUMBKgyo
Evolution of immune system
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-II.pdf
You Tube Link https://www.youtube.com/watch?v=i3OIsowiXmY
Immunological Memory
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-IX.pdf
YouTube Link https://www.youtube.com/watch?v=VWeoL37Jsbw
Immune response
PDF Link https://www.youtube.com/watch?v=LYZzvrg_qIM
YouTube Link https://www.youtube.com/watch?v=LYZzvrg_qIM
Lymphocytes
PDF Linkhttp://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-VII.pdf
YouTube Link https://www.youtube.com/watch?v=07S5VSeciOk
Ontogeny of Fish Immune System
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-VI.pdf
YouTube Link https://www.youtube.com/watch?v=cYluYpmAf54
Specific defence mechanism in Fish
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-V.pdf
YouTube Link https://www.youtube.com/watch?v=pi7LLW383k8&t=16s
Fish Leucocytes
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-IV.pdf
YouTube Link https://www.youtube.com/watch?v=f6o5sCFpX_0
Nonspecific immunity
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-III.pdf
You Tube Link https://www.youtube.com/watch?v=os1oh8AprYw
mmunologic tolerance
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-X.pdf
YouTube Link https://www.youtube.com/watch?v=CPidGGljMp0
Stress and immune response
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-XI.pdf
YouTube Link https://www.youtube.com/watch?v=2y54zacDe_8
Defence mechanisms in crustaceans
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-XII.pdf
Immuno-stimulants
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-XIII-Immuno-stimulants.pdf
YouTube Link https://www.youtube.com/watch?v=KHwcvOMOdvY
Vaccine
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-XIV.pdf
YouTube Link https://www.youtube.com/watch?v=YlKLpQEWILg&t=57s
Vaccination Methods
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-XV.pdf
YouTube Link https://www.youtube.com/watch?v=ccT8B37nYRI
Adjuvant
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/Chapter-XVI.pdf
YouTube Link https://www.youtube.com/watch?v=f9gMSBsk7qc
Collection, separation and identification of fish leucocytes
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/1.-Isolation-of-fish-leucocytes.pdf
YouTube Link https://www.youtube.com/watch?v=NMVFvY3znBA
Lymphocyte separation from fish blood
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/2.-lEUCOCYTE-iSOLATION.pdf
YouTube Link https://www.youtube.com/watch?v=LkqNUHz39F8
Differential counting - RBC and WBC by Haemocytometer (Neubauer Chamber)
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/3.Cell-count-RBCWBC.pdf
YouTube Link https://www.youtube.com/watch?v=MKS0KM3lr90
Separation of Blood Plasma and Serum
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/4.-separation-of-plasma-and-serum.pdf
YouTube Link https://www.youtube.com/watch?v=XAhBzUosvsU&t=154s
Latex Agglutination Test
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/5.-Latex-Agglutination-test.pdf
YouTube Link https://www.youtube.com/watch?v=3W67OH3v2lU&t=231s
Ouchterlony Double Immunodiffusion Test
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/6.-Double-Immunodiffusion-Test.pdf
YouTube Link https://www.youtube.com/watch?v=4vgyrT0U7S8
Antigen Capture Elisa Test
PDF Link http://courseware.cutm.ac.in/wp-content/uploads/2020/05/7.-Antigen-Capture-ELISA-Test.pdf
YouTube Link https://www.youtube.com/watch?v=YJ0-qQslqqQ&t=1s
Methods of Vaccine Preparation and Techniques of Fish Immunization
YouTube Link https://www.youtube.com/watch?v=W-Zi5TitJzk
Link http://ecoursesonline.iasri.res.in/course/view.php?id=255
http://www1.mans.edu.eg/FacMed/dept/microbiology/pdf/4-Basic-Immunology.pdf
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