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Integrate the basic biological and chemical knowledge and its structure to develop a foundation in the concepts and facts in modern cell and molecular biology and biochemistry, and to be familiar with various ways of organizing and accessing scientific knowledge
Student will understand and demonstrate fundamental biochemical principles, such as the structure/function of biomolecules, metabolic pathways, and the regulation of biological/biochemical processes
Theory
1. Cell structure, water and major molecules of life.
2. Carbohydrate chemistry: Structure, classification, functions (mono, di and polysaccharides) isomerism and mutarotation.
3. Metabolism of carbohydrates: glycolysis
4. Metabolism of carbohydrates: gluconeogenesis,
5. Metabolism of carbohydrates: glycogenolysis
6. Metabolism of carbohydrates: glycogenesis
7. TCA cycle, central role of TCA cycle in metabolism.
8. Protein chemistry: classifications and functions.
9. Primary, secondary, tertiary and quaternary structure of proteins. Amphoteric property. Biuret reaction and xanthoproteic reaction.
10. Digestion and absorption of proteins.
11. Classification, structure, function and properties of amino acids. Essential and non essential amino acids.
12. Classification, structure, functions and properties of lipids.
13. Essential fatty acids and phospholipids. Significance of Omega3 and Omega-6 fatty acids.
14. Digestion and absorption of lipids. Lipid autooxidation.
15. Enzymes: nomenclature; classification; specificity; mechanism of enzyme action; kinetics and regulation of enzyme activity.
16. Steroid and peptide hormones- chemistry and function.
17. Structure and functions of fat and water soluble vitamins. Vitamins – classification- functions.
18. Minerals – classification – functions.
19. Nucleic acids: Structure function and importance genetic code.
20. Transcription and translation. Protein synthesis.
Practical
1. Preparation of normal solution of acid and base, buffers and reagents.
2. Qualitative determination of carbohydrates, proteins and lipids.
3. Estimation of total nitrogen and crude protein of fish tissue.
4. Estimation of carbohydrates in foods.
5. Determination of specific gravity of oil. Extraction and estimation of total lipids in fish tissue.
6. Determination of saponification value
7. Determination of iodine value and free fatty acid value.
References
1. Brody, T. 2006.Nutritional Biochemistry 2nd Edition. Elsevier, India Pvt. Ltd. New Delhi.
2. Conn , E. E., Stumpf, P. K., Bruening, G., and Doi, R. H. 2003. Outlines of Biochemistry 5th edition. John Wiley and sons, Inc., New Jersey
3. Chatterjee, M. N., 2004. Text Book of Biochemistry for dental/nursing/pharmacy students, 2nd Edn. Jaypee Brothers medical publishers (P) Ltd. New Delhi. India.
4. Hames D and Hooper N., Bios 2008. Instant notes -Biochemistry, 3rd Edn. Taylore and Francis. USA.
5. Lehninger, A.L (Ed.): 1975. Biochemistry- 2nd edition, Worth publishers, Inc. New York.
6. Lehringer, David L. Nelson and Michael M. Cox,. 2000. Principle of Biochemistry, 3rd edition. Macmillan worth Publisher, New York, USA ...
7. Malhotra, V. K. 2003. Biochemistry for Students. 11th Edn. Jaypee Brothers Medical Publishers (P) Ltd. New Delhi.
8. Rambabu, K. 2008 Text book of Biochemistry, AITBS Publishers, India
9. Robert K. M, Daryl K G, Victor W R. 2006., Harper’s Illustrated Biochemistry, 27th International Edn., The McGraw. Hill companies, Inc.
10. Rostogi, S. C. 2004. Biochemistry 2nd Edn. Tata McGraw- Hill Publishing Company Limited, New Delhi
11. Saini, A S. 1994.Textbook of Biochemistry. CBS publishers and Distributors. New Delhi
12. Satyanarayana, U., and Chakrapani, U. 2006. Biochemistry 3rd edition. Books and Allied (p) Ltd. UK.
13. Satyanarayanan, U. 2009. Biotechnology. Books and Allied (P) Ltd. Kolkata
14. Trehan, K. 2003. Biochemistry – 2nd edition. New Age International (P) Ltd., New Delhi
15. Vasudevan D M and Sreekumari S. 2005.Text book of Biochemistry (for medical students), 4th Edn. Jaypee Brothers Medical publishers (P) Ltd. New Delhi.
16. West, E. S., Todd, W., Mason, H. S., Bruggen, J. T. V. 1996. Text book of Biochemistry, 4th Edn. Oxford and IBH publishing Co. Pvt. Ltd. New Delhi.
17. Fatima, D., Meyyan, R. P., Prasanna Kumar, S., Narayanan, L. M., Nallasingam, K, and Arumugam, N. 2005. Biochemistry. Saras publication, Nagercoil, T.N..
Cell structure, water and major molecules of life
Structure and functions of cell
Practical - Estimation of carbohydrates in foods
Practical - Preparation of normal solution of acid and base, buffers and reagents
Practical - Qualitative determination of carbohydrates, proteins and lipids.
TCA cycle, central role of TCA cycle in metabolism
TCA cycle, central role of TCA cycle in metabolism
Protein chemistry: classifications and functions
Protein chemistry classifications and functions
Practical - Estimation of total nitrogen and crude protein of fish tissue.
Practical - Determination of specific gravity of oil. Extraction and estimation of total lipids in fish tissue
Essential fatty acids and phospholipids. Significance of Omega3 and Omega-6 fatty acids
Essential fatty acids and phospholipids. Significance of Omega3 and Omega-6 fatty acids.
Practical - Determination of saponification value
Practical - Determination of iodine value
Practical - Determination of free fattyacid value
Transcription and translation. Protein synthesis
Transcription and translation. Protein synthesis
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