Fundamentals of biochemistry

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PANKAJ MEHER

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Core Courses

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An in depth study for better application of mind to further approach towards biology.

This course would make students well equipped with the process of body and its functioning.

Course Name :FUNDAMENTALS OF BIOCHEMISTRY

 

Code(Credit) : CUTM1503(3-2-1)

 

Course Objectives

  • This course will make students to know about the biochemical features in organisms and self.

Learning Outcomes

  • An in depth study for better application of mind to further approach towards biology.

  • This course would make students well equipped with the process of body and its functioning.

Course Syllabus

Module-I

Carbohydrates: Structure and Biological importance of :Monosaccharides,Disaccharides,Polysaccharides and Glycoconjugates

Assignment- prepare a note on carbohydreate.

Practice

  1. Qualitative tests of functional groups in carbohydrates

Module-II

Lipids: Structure and Significance: Physiologically important saturated and    unsaturated fatty acids,Tri-acylglycerols,Phospholipids,Glycolipids,Steroids

Assignment- prepare a note on lipids.

Practice:-

  1. Qualitative tests of functional groups in lipids.

Module-III

Proteins: Amino acids: Structure, Classification and General properties of α-amino acids;Physiological importance of essential and non-essential α-amino acidsProteins:Bonds stabilizing protein structure and Denaturation;Levels of organization in proteins;Introduction to simple and conjugate proteins

Assignment- prepare a note on prortein.

Practice:-

  1. Qualitative tests of functional groups in protein

  2. Separation of amino acid using Paper Chromatography.

Module-IV

Immunoglobulins: Basic Structure, Classes and Function, Antigenic Determinants.

Nucleic Acids: Structure: Purines and pyrimidines, Nucleosides, Nucleotides, Nucleic acids;Cot Curves: Base pairing, Denaturation and Renaturation of DNA;Types of DNA and RNA;Complementarity of DNA;Hpyo-Hyperchromaticity of DNA.

Assignment- prepare a note on nucleic acid.

Practice:-

  1. Quantitative estimation of salmon sperm/calf thymus DNA using colorimeter(Diphenylamine reagent) or spectrophotometer (A260 measurement)

Module-V

Enzymes: Nomenclature and classification;Cofactors;Specificity of enzyme action;Isozymes;Mechanism of enzyme action.

Assignment- prepare a note on Enzyme.

Practice:-

  1. Action of salivary amylase under optimum conditions.

  2. Effect of different pH on salivary amylase activity.

Module-VI

Enzyme kinetics;Factors affecting rate of enzyme-catalyzed reactions;Derivation of Michaelis-Menten equation;Concept of Km and Vmax, and Lineweaver-Burk plot;Multi- substrate reactions.

Assignment- prepare a note on feedback mechanism of enzyme.

Practice:-

  1. Demonstration of proteins separation by SDS-PAGE.

Module-VII

Enzyme inhibition;Allosteric enzymes and their kinetics;Regulation of enzyme action.

Assignment- prepare a note on allosteric enzyme.

practice:-

  1. Effect of different Temperature on salivary amylase activity.

  2. Effect of inhibitor on salivary amylase activity

Text Books:

Cox, M.M and NelsoEXT n, D.L. (2008). Lehninger’s Principles of Biochemistry, V Edition, W.H. Freeman and Co., New York.

Reference Books:

  1. Cox, M.M and NelsoEXT n, D.L. (2008). Lehninger’s Principles of Biochemistry, V Edition,

  2. W.H. Freeman and Co., New York.

  3. Berg, J.M., Tymoczko, J.L. and Stryer, L. (2007). Biochemistry, VI Edition, W.H. Freeman and Co., New York.rs

  4. Sources of Environmental hazards, hazard identification and accounting, fate of toxic and persistent substances in the environment, dose Response Evaluation, exposure Assessment.

Session 1

Carbohydrates: Structure and Biological importance of : Monosaccharides

https://www.youtube.com/watch?v=p0soyMv8-Xo

session 5(Assignment)

prepare a note on lipids.

session 6(Practice)

Qualitative tests of functional groups in carbohydrates,

http://vlab.amrita.edu/?sub=3&brch=63&sim=631&cnt=1

Session 7

Lipids: Structure and Significance:Physiologically important saturated and unsaturated fatty acids

https://www.youtube.com/watch?v=brs2nMubr84

Session 8

Session 12(Assignment)

Prepare a note on prortein.

session 13(Practice)

  1. Qualitative tests of functional groups in lipids.

https://www.youtube.com/watch?v=n4esSHxz_J8

Session 14

Proteins: Amino acids:

Structure, Classification and General properties of α-amino acids

https://www.youtube.com/watch?v=9Qi3225yrmw

Session 15

Physiological importance of essential and non-essential α-amino acids Proteins

https://www.youtube.com/watch?v=CESILJr2zg4

Session 16

Bonds stabilizing protein structure and Denaturation;

https://www.youtube.com/watch?v=Fff2wy4ugeY

Session 17

Levels of organization in proteins;

https://www.youtube.com/watch?v=hok2hyED9go

Session 18

Introduction to simple and conjugate proteins

https://www.youtube.com/watch?v=CKemmavZAKQ

Session 19(Assignment)

Prepare a note on nucleic acid.

session 20

  1. Qualitative tests of functional groups in protein

http://vlab.amrita.edu/?sub=3&brch=63&sim=1094&cnt=1

  1. Separation of amino acid using Paper. Chromatography .

https://www.youtube.com/watch?v=23W5Z_redfs

Session 21

Immunoglobulins: Basic Structure, Classes and Function of antibody, Antigenic Determinants.

https://www.youtube.com/watch?v=FHLyvp_LVK4

https://www.youtube.com/watch?v=XSeaG-UHYIQ

Session 22

Nucleic Acids:

Structure: Purines and pyrimidines, Nucleosides, Nucleotides, Nucleic acids

https://www.youtube.com/watch?v=RA9n0Enu5Gw

https://www.youtube.com/watch?v=0lZRAShqft0

Session 23

Cot Curves: Base pairing, Denaturation and Renaturation of DNA

https://www.youtube.com/watch?v=oARK37UhLiY

https://www.youtube.com/watch?v=Y6JLCYa04mg

Session 25

Session 26

Hpyo-Hyperchromaticity of DNA

https://www.youtube.com/watch?v=dcLeJ_pDCCc

Session 27(Assignment)

prepare a note on Enzyme.

Session 28 (Practice)

  1. Quantitative estimation of salmon sperm/calf thymus DNA using colorimeter(Diphenylamine reagent) or spectrophotometer (A260 measurement)

https://www.youtube.com/watch?v=Q799WUT8VQo

Session 30

Specificity of enzyme action;

https://www.youtube.com/watch?v=Q_25_U870xg

Session 31

Session 32

Mechanism of enzyme action

https://www.youtube.com/watch?v=MHN9FLuJtXY

Session 33(Assignment)

Prepare a note on feedback mechanism of enzyme.

session 34(Practice)

Action of salivary amylase under optimum conditions.

https://www.youtube.com/watch?v=iaqxTaXit9M

Effect of different pH on salivary amylase activity.

https://www.youtube.com/watch?v=JUQExVhugFI

Session 35

Session 36

Factors affecting rate of enzyme-catalyzed reactions;

https://www.youtube.com/watch?v=uHQp-oXq8So

Session 37

Derivation of Michaelis-Menten equation and Concept of Km and Vmax, Lineweaver-Burk plot;

https://www.youtube.com/watch?v=8PWF5OeB7Ec

https://www.youtube.com/watch?v=59chK70akoo

https://www.youtube.com/watch?v=4aVOtcpGaZc

Session 38

Multi- substrate reactions

https://www.youtube.com/watch?v=KueNVfv8fNY

session 39(Assignment)

  1. Demonstration of proteins separation by SDS-PAGE

https://www.youtube.com/watch?v=i_6y6Z5UvwE

Session 40(Practice)

  1. Demonstration of proteins separation by SDS-PAGE

https://www.youtube.com/watch?v=i_6y6Z5UvwE

Session 41

Session 42

Allosteric enzymes and their kinetics and Regulation of enzyme action

https://www.youtube.com/watch?v=DuSinQd9YhA

Session 43(Assignment)

prepare a note on allosteric enzyme

Session 44(Practice)

Effect of different Temperature on salivary amylase activity.

https://www.youtube.com/watch?v=iaqxTaXit9M

Effect of inhibitor on salivary amylase activity

https://www.youtube.com/watch?v=lXF6yjdyIgY

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