FUNDAMENTALS OF PLANT BIOCHEMISTRY

Teacher

Dr. Goutam Kumar Dash

Category

Core Courses

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Course Name :FUNDAMENTALS OF PLANT BIOCHEMISTRY

Code(Credit) : ASBC2101:3(2-1-0)

Prepared By : Dr. Goutam Kumar Dash

Teachers: Dr. Koustava Kumar Panda, Dr. Poulami Sil, Dr. Panchashree Das

Program Outcome (PO)

PO1 : Knowledge Enhancement : Demonstrate knowledge and understanding of essential facts, concepts, principles and theories relating to Soil Science, Agronomy, Horticulture, Genetics and Plant Breeding, Plant Pathology, Entomology and other such areas of agriculture.

PO2 : Professional Enhancement : Understanding the value and processes of life-long learning and professional development.

Course Objectives:

Understand the fundamental principles and concepts of plant biochemistry, including the structure and function of biological macromolecules such as proteins, carbohydrates, lipids, and nucleic acids, explore the various metabolic pathways and processes and kinetics of enzymatic reactions and their regulation in plants.

Course Outcome (CO)

  1. CO1: Students will apply the knowledge and principles of plant biochemistry to analyze and understand the biochemical processes and metabolic pathways involved in crop growth, development, metabolism and stress responses.
  2. CO2: Become familiar with qualitative and quantitative tests and data analysis for important biomolecules, as well as the professional operation of various equipment and reagents related to basic plant biochemical profiling.

CO & PO mapping table

COs Course outcomes Mapping COs with POs
CO1 Students will apply the knowledge and principles of plant biochemistry to analyze and understand the biochemical processes and metabolic pathways involved in crop growth, development, metabolism and stress responses. PO1
CO2 Become familiar with qualitative and quantitative tests and data analysis for important biomolecules, as well as the professional operation of various equipment and reagents related to basic plant biochemical profiling.  

PO2

 

 

PO1 : Knowledge Enhancement

PO2 : Professional Enhancement

Course Syllabus:

Theory:
Importance of Biochemistry

Properties of Water, pH and Buffer.

Carbohydrate: Importance and classification. Structures of Monosaccharides, Reducing and oxidizing properties of Monosaccharides, Mutarotation; Structure of Disaccharides and Poly saccharides.

Lipid: Importance and classification; Structures and properties of fatty acids; storage lipids and membrane lipids.

Proteins: Importance of proteins and classification; Structures, titration and zwitterions nature of amino acids; Structural organization of proteins.

Enzymes: General properties; Classification; Mechanism of action; Michaelis & Menten and Line Weaver Burk equation & plots; Introduction to allosteric enzymes.

Nucleic acids: Importance and classification; Structure of Nucleotides, A, B & Z DNA; RNA: Types and Secondary & Tertiary structure.

Metabolism of carbohydrates: Glycolysis, TCA cycle, Glyoxylate cycle, Electron transport chain.

Metabolism of lipids: Beta oxidation, Biosynthesis of fatty acids.

 

Practical:
Preparation of solution, pH & buffers,

Qualitative tests of carbohydrates and amino acids.

Quantitative estimation of glucose/ proteins.

Titration methods for estimation of amino acids,

Effect of pH, temperature and substrate concentration on enzyme action,

Paper chromatography/ TLC demonstration for separation of amino acids/ Monosaccharides.

Sterilization techniques.

References:

1. Satyanarayana U,Textbook of biochemistry,2007, 3rd edition, Books and Allied (P) ltd, Kolkata.
2. Lehninger, Albert L., David L. Nelson, and Michael M. Cox. 2000. Lehninger principles of biochemistry. New York: Worth Publishers.
3. D T Plummer, An Introduction to Practical Biochemistry, 1987, 3rd edition,McGraw-Hill, USA.
4. S. Sadasivam and A. Manickam, “Biochemical Methods,” New Age International (P) Limited, New Delhi, Vol. 2. 1996, pp. 124-126.

Theory Session Plan

Theory Session 1

Theory Session 2

Theory session 4

Buffer: Preparation of acidic and basic buffers and their importance

Buffer-solution

https://youtu.be/LiEXkUkKQQA

https://youtu.be/pN2BxjeX35Y

Theory Session 5

Theory Session 7

Two families of monosaccharides: Aldoses and Ketoses: Glyceraldehyde and dihydroxyacetone

CARBOHYDRATES

https://youtu.be/42llr6Bj0EE

https://www.youtube.com/watch?v=wFYsufJ9XMM

https://www.youtube.com/watch?v=LeOUIXbFyqk

Theory Session 8

Tautomers, epimers, anomers, asymmetric carbon

Tautomers

https://youtu.be/Ea9yDCzTLao

Theory Session 9

Reducing and oxidizing properties of monosaccharides, mutarotation

CARBOHYDRATES

Tautomers

https://youtu.be/pqVYeXAhPtU

https://youtu.be/Omzi1GNnPko

Theory Session 32

Practical Session Plan

Practical Session 1

Practical Session 3

Practical  Session 4

Practical Session 5

Sterilization techniques: Moist heat, dry heat, flame, chemically mediated, UV mediated, filter sterilization

Sterilization

https://youtu.be/KRL7Bt3F38k

https://youtu.be/bXX0kDdeEks

https://youtu.be/iyiaH_vhVgU

https://youtu.be/GjHSJT90Omw

Practical Session 6

Practical Session 7

Practical Session 8

Titration method for the estimation of amino acids

Titration method

https://youtu.be/2vInVzyXluo

Practical Session 9

Qualitative tests for carbohydrates: Molisch's test

Qualitative tests for carbohydrates

https://youtu.be/ojhdTFmkY1c

Practical Session 10

Qualitative tests for carbohydrates: Fehling's test

Qualitative-tests-for-carbohydrates

https://youtu.be/ojhdTFmkY1c

Practical Session 11

Qualitative tests for carbohydrates: Benedict's & Barfoed's Mucic test

 

Qualitative-tests-for-carbohydrates

https://youtu.be/ojhdTFmkY1c

Practical Session 12

Qualitative tests for carbohydrates: Seliwanoff's and Bial's test

Qualitative-tests-for-carbohydrates

https://youtu.be/ojhdTFmkY1c

Practical Session 13

Qualitative tests for carbohydrates: Test for Non reducing sugar (Mucic acid test & Osazone test)

Qualitative-tests-for-carbohydrates

https://youtu.be/ojhdTFmkY1c

Practical Session 14

Enzyme kinetics: study of competitive inhibition

Enzyme kinetics study of competitive inhibition

Practice Session 16

Study of the effect of PH, temperature and substrate concentration on enzyme activity

effect-of-ph-and-temperature-on-enzyme-activity-notes

Practice-7

https://youtu.be/ylhA84Uyb_A

https://www.youtube.com/watch?v=UQww6s-64wo

Assignments

  1. DNA structure and forms of DNA
  2. Structure of 20 amino acids
  3. Secondary and tertiary structure of RNA
  4. Michaelis- Menten equation, Lineweaver- Burk plots
  5. Glycolysis & TCA cycle and their significance
  6. Electron transport chain of mitochondria
  7. Carbohydrate structure and classification
  8. Structure and properties of fatty acids
  9. Properties of amino acids: Zwitterion, isoelectric PH, titration curve for amino acids