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THEORY
1. Scopes offered by Plant Biotechnology, Importance of Plant Tissue Culture;
2. Different types of cultures carried out under Plant Tissue Culture and their importance;
3. Callus culture, organ culture, embryo culture and their importance;
4. Cell Suspension culture, Anther culture, Pollen culture, Ovule culture: Importance of carrying out such cultures;
5. Synthetic seeds: Production and their significance;
6. Embryo rescue and its significance;
7. Somatic hybridization: Protoplast isolation, Fusion, selection of somatic hybrids, Cybrids and their role;
8. Somaclonal Variation and its role in crop improvement;
9. Cryopreservation: Importance and the role of Cryoprotectants;
10. Recombinant DNA Technology/Gene cloning: Steps involved ;
11. Vector based and vector-less approaches for gene delivery;
12. Transgenic Plants as Potential bioreactors;
13. PCR technique and its applications;
14. Markers (RFLP, RAPD, SSR) and their roles in Agricultural biotechnology;
15. Marker assisted selection for mapping agronomic traits;
16. Guidelines to be followed with respect to genetic engineering practices, role of different organizations like DBT, GEAC in monitoring genetic engineering practices;
LABORATORY
References:
Session 1
Historical developments in Plant Biotechnology along with the scopes offered by it
https://www.slideshare.net/prakashsiju/chapter-3-plant-tissue-culture
The session introduces one to the historical developments in Plant Biotechnology along with the scopes offered by it
protoplast culture, callus culture, organ culture, embryo culture, cell suspension culture, anther culture, pollen culture, ovule culture and their importance
Session 3
Embryo rescue and its significance
https://www.slideshare.net/RIZWANABBAS3/embryo-culture-application-
Session 4
Somatic hybridization and its importance
https://www.slideshare.net/neha_ag/somatic-hybridization-67740744
https://www.slideshare.net/devhingra/somatic-hybridization-60180584
Session 5
Micropropagation in tissue culture
Session 6
Somaclonal variation and its role in crop improvement
https://www.slideshare.net/RakeshKumar440/somaclonal-variation-26537134
Session 7
Synthetic seeds: Production and their significance
https://www.slideshare.net/SonamkzBhutia/artificial-seed-98432308
Session 8
Steps involved in gene cloning
https://www.slideshare.net/gohilsanjay3/gene-cloning-principles-an-technique
Session 9
Vector based approaches for gene delivery
https://www.slideshare.net/NISHANTHSEKAR1/agrobacterium-mediated-gene-transfer-77002058
Session 10
Vectorless approaches for gene delivery
https://www.slideshare.net/manjeshsaakre/vector-less-gene-transfer
Session 11
Polymerase chain reaction and its applications
https://www.slideshare.net/subramaniansethupath/polymerase-chain-reaction-principles-and-practice
Session 12
Molecular markers: Construction and uses
https://www.slideshare.net/VikasSingh639/molecular-markers-75788487
Session 13
Organizations involved in monitoring genetic engineering practises
https://www.slideshare.net/Adrienna/national-and-international-regulatory-framework-for-gm-crops2015
Session 14
Organization of plant tissue culture laboratory
Session 16
Preparation of stock solutions of MS nutrient medium
Session 17
Preparation of MS nutrient medium and its sterilization
Session 19
Isolation of plant genomic DNA and its spectrophotometric quantification
Session 22
Visit to high tech polyhouse for studying the factors contributing to plant growth
I am Dr. Koustava Kumar Panda presently working as an Associate Professor in the department of Plant Biotechnology, M.S.Swaminathan School of Agriculture, Centurion university, Paralakhemundi since July 2017. I have worked on the the nutritional profiling of prominent rice landraces of Southern Odisha on the elemental, mineral, nurtitional quality attributes in addition to studying their […]