INSTRUMENTAL METHODS OF ANALYSIS (Practical)

Teacher

Mr. HARA GOURI MISHRA

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Core Courses

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Course Name : INSTRUMENTAL METHODS OF ANALYSIS (Practical)

Code(Credit) : BPHL4101(0-2-0)

Course Objectives

This subject deals with the application of instrumental methods in qualitative and quantitative analysis of drugs. This subject emphasizes on practical knowledge of modern analytical instruments that are used for drug testing.

Learning Outcomes

  1. Understand the interaction of matter with electromagnetic radiations and its applications in drug analysis.
  2. Understand the chromatographic separation and analysis of drugs.
  3. Perform quantitative & qualitative analysis of drugs using various analytical
    instrument

Course Syllabus

1 Determination of absorption maxima and effect of solvents on absorption
maxima of organic compounds
2 Estimation of dextrose by colorimetry
3 Estimation of sulfanilamide by colorimetry
4 Simultaneous estimation of ibuprofen and paracetamol by UV spectroscopy
5 Assay of paracetamol by UV- Spectrophotometry
6 Estimation of quinine sulfate by fluorimetry
7 Study of quenching of fluorescence
8 Determination of sodium by flame photometry
9 Determination of potassium by flame photometry
10 Determination of chlorides and sulphates by nephelo turbidometry
11 Separation of amino acids by paper chromatography
12 Separation of sugars by thin layer chromatography
13 Separation of plant pigments by column chromatography
14 Demonstration experiment on HPLC
15 Demonstration experiment on Gas Chromatography

Recommended Books:

1.Vogel’s Text book of Quantitative Chemical Analysis by A.I. Vogel

2.Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake

Session Plan

Experiment 1

Determination of absorption maxima and effect of solvents on absorption maxima of organic compounds

Effects of solvents on absorption maxima

Determination of absorption maxima

Experiment 2

Estimation of dextrose by colorimetry

Estimation of dextrose by Colorimetry

Experiment 3

Estimation of sulfanilamide by colorimetry

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Handling of colorimeter to estimate sulphanilamide

Experiment 4

Experiment 6

Estimation of quinine sulfate by fluorimetry

Fluorescence Quinine sulphate

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Experiment 7

Study of quenching of fluorescence

Study of quenching of fluoroscence

FluorescenceQuenching

Experiment 8

Determination of sodium by flame photometry

Estimation of Sodium by flame photometry

Experiment 9

Determination of potassium by flame photometry

Determination of Potassium by flame photometry

Experiment 10

Determination of chlorides and sulphates by nephelo turbidometry

Turbid metric determination of chloride

Determination of Sulphate by nephloturbidimetry

Experiment 11

Separation of amino acids by paper chromatography

SEPARATION OF AMINO ACIDS BY PAPER CHROMATOGRAPHY

Experiment 12

Experiment 13

Separation of plant pigments by column chromatography

Separation of plant pigments by column chromatography

Experiment 14

Demonstration experiment on HPLC

Assay of Azithromycin by HPLC in tablet dosage form

Experiment 15

Demonstration experiment on Gas Chromatography

Gas chromatography

Gas chromatography

Case Studies

Case Studies

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