Introduction to biotechnology and Bioinformatics

Teacher

Gulshan Kumar

Category

Core Courses

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Course Name : Introduction to biotechnology and Bioinformatics

Code(Credit) : FSRM 3210(1-1-0)

Course Objectives

  • To learn various biotechnological applications for enhancing production through sustainable eco-friendly culture.

Learning Outcomes

  • Student gets a vivid idea about biotechnological tools used in aquaculture.

Course Syllabus

Theory
Biotechnology: Introduction to Biotechnology –scope and importance in fisheries/aquaculture; Structural organization of prokaryotic and eukaryotic cell. Nucleic acids -structure, function and types, Concepts of gene and genetic code, transcription and translation, mutations and their implications. Post transcriptional modification and RNA processing. Gene regulation and expression in prokaryotes and eukaryotes; DNA sequencing, Operons. Genetic engineering- Restriction enzymes; Gene isolation; Cloning vectors; Probes; Recombinant DNA technology – vaccines. Transgenic fish and Gene transfer technology, Animal Cell Culture, Hybridoma technology. Molecular and immunological techniques – PCR; immunoblotting; ELISA; Principle of hybridization; Northern blotting; Western blotting; Southern blotting; DNA fingerprinting; Restriction fragment length polymorphism., Biosensors. Concept of bioremediation of water, bioprocess engineering and bioprospecting.Genomics
Bioinformatics: Introduction to Bioinformatics; Biological Databases and tools : Introduction; Types of biological databases; Primary and secondary databases; PDB, NCBI, formats and contents; Sequence retrieval, manipulation; Primer design; Restriction mapping; ORF finding; EMBOSS, Molecular visualization Sequence analysis.

Practicals
Study of structure of prokaryot and Eukaoryt Cells. Study on Model of protein Synthesis, Study of models rDNA Technology, Cell Culture, Isolation of Nucleic Acids, Restriction enzymes, Gel Electrophorus, ELISA, DNA sequence analysis and comparison.

Suggested Reading

Fish Genetics & Biotechnology ---- W.S.Lakra

Session Plan

Session 1

Session 1. Theory: Introduction to Biotechnology –scope and importance in fisheries/aquaculture

Session 2

Session 2. Practice: Structural comparision of plant and animal cell

Session 3

Session 3. Theory: Structural organization of prokaryotic and eukaryotic cell. Nucleic acids -structure, function and types.

Session 4

Session 4. Practice: Isolation of genomic DNA from fish tissue

Session 5

Session 5. Theory: Concepts of gene and genetic code, transcription and translation, mutations and their implications.

Session 6

Session 6. Practice: Isolation of total RNA from fish tissue

Session 7

Session 7. Theory: Post transcriptional modification and RNA processing. Gene regulation and expression in prokaryotes and eukaryotes, operons.

Session 8

Session 8. Practice: Isolation of plasmid DNA and gel electrophoresis.

Session 9

Session 9. Theory: Genetic engineering- Recombinant DNA technology, Restriction enzymes; Gene isolation; Cloning vectors; Probes; DNA sequencing and genomics.

Session 10

Session 10. Practical: Recombinant DNA technology; overview of steps

Session 11

Session 11. Theory: Transgenic fish and gene transfer technology.

Session 12

Session 12. Practical: Microinjection steps.

Session 13

Session 13. Theory: Vaccines types and process of vaccination.

Video link

Recombinant vaccine

Session 14

Session 14. Practice: Vaccination in fish

Video link

Vaccination of fish

Session 15

Session 15. Theory: Animal Cell Culture.

Video link

Cell culture basics

Session 16

Session 16. Practice: Cell culture work flow.

Session 17

Session 17. Theory: Hybridoma technology and production of monoclonal antibodies.

Session 18

Session 18. Practice: Hybridoma technology overview and selection techniques.

Session 19

Session 19. Theory: Molecular techniques nucleic acid based, PCR and hybridisation

Video link

PCR basics

Session 20

Session 20. Practice: Steps in conventional PCR.

Video link

PCR demo

Session 21

Session 21. Theory: Immunological techniques. ELISA and western blotting.

Video link

ELISA technique

Session 22

Session 22. Practice: Overview of ELISA.

Video link

ELISA demo

Session 23

Session 23. Theory: Biosensors. Concept of bioremediation of water, bioprocess engineering and bioprospecting.

Video limk

Biosensors

Session 24

Session 24. Practice: Bioprocess overview

Video link

Bioprocess overview

Session 25

Session 25. Theory: Bioinformatics introduction to biological databases.

Session 26

Session 26. Practice: Sequence and structural retrieval from NCBI and PDB databases.

Video link

NCBI demo

Case Studies

Case Studies

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