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Theory
Biotechnology: Introduction to Biotechnology –scope and importance in fisheries/aquaculture; Structural organization of prokaryotic and eukaryotic cell. Nucleic acids -structure, function and types, Concepts of gene and genetic code, transcription and translation, mutations and their implications. Post transcriptional modification and RNA processing. Gene regulation and expression in prokaryotes and eukaryotes; DNA sequencing, Operons. Genetic engineering- Restriction enzymes; Gene isolation; Cloning vectors; Probes; Recombinant DNA technology – vaccines. Transgenic fish and Gene transfer technology, Animal Cell Culture, Hybridoma technology. Molecular and immunological techniques – PCR; immunoblotting; ELISA; Principle of hybridization; Northern blotting; Western blotting; Southern blotting; DNA fingerprinting; Restriction fragment length polymorphism., Biosensors. Concept of bioremediation of water, bioprocess engineering and bioprospecting.Genomics
Bioinformatics: Introduction to Bioinformatics; Biological Databases and tools : Introduction; Types of biological databases; Primary and secondary databases; PDB, NCBI, formats and contents; Sequence retrieval, manipulation; Primer design; Restriction mapping; ORF finding; EMBOSS, Molecular visualization Sequence analysis.
Practicals
Study of structure of prokaryot and Eukaoryt Cells. Study on Model of protein Synthesis, Study of models rDNA Technology, Cell Culture, Isolation of Nucleic Acids, Restriction enzymes, Gel Electrophorus, ELISA, DNA sequence analysis and comparison.
Suggested Reading
Fish Genetics & Biotechnology ---- W.S.Lakra
Session 1. Theory: Introduction to Biotechnology –scope and importance in fisheries/aquaculture
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Session 2. Practice: Structural comparision of plant and animal cell
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Session 3. Theory: Structural organization of prokaryotic and eukaryotic cell. Nucleic acids -structure, function and types.
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Session 4. Practice: Isolation of genomic DNA from fish tissue
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Session 5. Theory: Concepts of gene and genetic code, transcription and translation, mutations and their implications.
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Session 6. Practice: Isolation of total RNA from fish tissue
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Session 7. Theory: Post transcriptional modification and RNA processing. Gene regulation and expression in prokaryotes and eukaryotes, operons.
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Session 8. Practice: Isolation of plasmid DNA and gel electrophoresis.
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Session 9. Theory: Genetic engineering- Recombinant DNA technology, Restriction enzymes; Gene isolation; Cloning vectors; Probes; DNA sequencing and genomics.
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Session 10. Practical: Recombinant DNA technology; overview of steps
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Session 11. Theory: Transgenic fish and gene transfer technology.
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Session 13. Theory: Vaccines types and process of vaccination.
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Session 15. Theory: Animal Cell Culture.
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Session 17. Theory: Hybridoma technology and production of monoclonal antibodies.
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Session 18. Practice: Hybridoma technology overview and selection techniques.
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Session 19. Theory: Molecular techniques nucleic acid based, PCR and hybridisation
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Session 21. Theory: Immunological techniques. ELISA and western blotting.
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Session 23. Theory: Biosensors. Concept of bioremediation of water, bioprocess engineering and bioprospecting.
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Session 25. Theory: Bioinformatics introduction to biological databases.
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Session 26. Practice: Sequence and structural retrieval from NCBI and PDB databases.
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