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Biotechnology: Introduction to Biotechnology –scope and importance in fisheries/aquaculture; Structural organization of prokaryotic and eukaryotic cell. Nucleic acids -structure, function and types, Concepts of gene and genetic code, transcription and translation, mutations and their implications. Post transcriptional modification and RNA processing. Gene regulation and expression in prokaryotes and eukaryotes; DNA sequencing, Operons. Genetic engineering- Restriction enzymes; Gene isolation; Cloning vectors; Probes; Recombinant DNA technology – vaccines. Transgenic fish and Gene transfer technology, Animal Cell Culture, Hybridoma technology. Molecular and immunological techniques – PCR; immunoblotting; ELISA; Principle of hybridization; Northern blotting; Western blotting; Southern blotting; DNA fingerprinting; Restriction fragment length polymorphism., Biosensors. Concept of bioremediation of water, bioprocess engineering and bioprospecting.Genomics
Bioinformatics: Introduction to Bioinformatics; Biological Databases and tools : Introduction; Types of biological databases; Primary and secondary databases; PDB, NCBI, formats and contents; Sequence retrieval, manipulation; Primer design; Restriction mapping; ORF finding; EMBOSS, Molecular visualization Sequence analysis.
Study of structure of prokaryot and Eukaoryt Cells. Study on Model of protein Synthesis, Study of models rDNA Technology, Cell Culture, Isolation of Nucleic Acids, Restriction enzymes, Gel Electrophorus, ELISA, DNA sequence analysis and comparison.
Fish Genetics & Biotechnology ---- W.S.Lakra
Session 23. Theory: Biosensors. Concept of bioremediation of water, bioprocess engineering and bioprospecting.