Introduction to Biotechnology (CUTM 2367) (3-0-0)

Teacher

Dr. Ranjan Kumar Sahoo

Category

Core Courses

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COs Course outcomes Mapping COs with POs (High-3, Medium-2, Low-1)
CO1 Explain the definition, classification, biological function, structure and interactions of Biomolecules. PO1 ( 3)
CO2 Analyse the functioning of life at cellular level. PO2(2)
CO3 Discuss and differentiate the basic structure and function of cell components in prokaryotes and eukaryotes cells. Po3(2)

Course Objective

  • To introduce student’s basic knowledge about Biotechnology

Course Outcome

To impart a sound knowledge on the principles of Biotechnology involving the different application oriented topics required for all engineering branches

Module I: Biochemistry 

Component of the cell, structure and biochemical functions

 

Module II:  Biomolecules    

Carbohydrates, Lipids, Proteins, Nucleic acids, Structure and classification of enzymes.

 

Module III:  Cell Biology

Eukaryotic and Prokaryotic cells, Cell cycle-Mitosis and Meiosis, Cell fractionation and flow     cytometry

 

Module IV:  Introduction to nucleic acids

Nucleic acids as genetic material, structure and physiochemical properties of elements in DNA and RNA, Biological significance of differences in DNA and RNA

 

Module V:  Immunology

 Cells of immune system, Development, maturation, activation and differentiation of T cells and B cells, Phagocytosis process

 

Module VI: Introduction to cloning

Overview of cloning, Purification and separation of Nucleic acids-cutting and joining DNA and vectors, analysis of gene expression, applications.

 

ModuleVII: Biotechnology    Application

Industrial production, Drug Discovery and development, GMO, Bioremediation.

 

List of Experiments:

  1. pH measurements and preparation of buffers.
  2. Microscopic study of cell and cell organelles
  3. Blood grouping
  4. DNA and RNA isolation
  5. Restriction enzyme digestion of plasmid DNA

 

Reference:

 

Text books:

 

  1. Lehninger A.L., Nelson D.L. and M.M. Principles of Biochemistry.CBS publishers and distributers.

 

  1. Murray R.K., Granner D.K., Mayes P.A and Rodwell V.W.Harpers Biochemistry. Appleton and Lange, Stanford, Conneticut.

 

 Reference Books:

 

  1. Lodish,Harvey et al., “Molecular Cell Biology”, 6th W.H Freeman,2008.

 

  1. Alberts,Bruce, “Molecular Biology of Cell”,5th  Edition, Garland  Science,2008.

 

  1. Satyanarayana,U. “Biotechnology” Books & Allied(P) Ltd.,2019.

 

  1. Friefelder, David. “Molecular Biology”.Narosa Publications, 2016.

Session 1

Component of the cell

http://https://www.youtube.com/watch?v=wE37VV5vI7I

Session 3

Biochemical functions of the cell

http://https://www.youtube.com/watch?v=tBvAMGeHsj8

Session 7

Structure and classification of enzymes

http://https://www.youtube.com/watch?v=NOimzeoRlCg

Session 10

Differences between Eukaryotic and Prokaryotic cells

http://https://www.youtube.com/watch?v=Pxujitlv8wc

Session 14

Cell fraction and Flow Cytometry

http://https://www.youtube.com/watch?v=Ut9f_d19wPc

Session 15

Nucleic acids as genetic material

http://https://www.youtube.com/watch?v=MA-ouz1LtpM

Session 16

Structure and physiochemical properties of elements in DNA and RNA

http://https://www.youtube.com/watch?v=SZ0vT27sStc

Session 17

Biological significance of differences in DNA and RNA

http://https://www.youtube.com/watch?v=2AdwRmRqP_g

Session 18

Cells of immune system

http://https://www.youtube.com/watch?v=bserR4ZDDAQ

Session 19

Development, maturation, activation and differentiation of T cells and B cells

http://https://www.youtube.com/watch?v=iIJulGXWm6Q

Session 20

Phagocytosis process

http://https://www.youtube.com/watch?v=Q84-mP0cGWQ

Session 24

http://https://www.youtube.com/watch?v=Hv5flUOsE0s

Analysis of gene expression, applications

Session 25

Industrial production

http://https://www.youtube.com/watch?v=gVjYM7ZnN6s

Session 30

Practice-2

Session 29

Practice-1

Our Main Teachers

Dr. Ranjan Kumar Sahoo

Associate Professor, Department of Biotechnology
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