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Microbial Biotechnology

Microbial Biotechnology

Teacher

Dr. Ranjan Kumar Sahoo

Category

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Course Name : Microbial Biotechnology

Code(Credit) : CUTM 1120(3-1-1)

COs

Course outcomes

Mapping COs with POs (High-3, Medium-2, Low-1)

CO1

 Knowledge of animal cells, basics of animal tissue culture technique, knowledge of media, and their fundamentals, preservations etc Understand the detailed functions of the animal cells and their intricate functions

PO1 (3)

CO2

Individual and team work: Students will be able to know the animal cell culture and its use for developing valuable products

PO9 (3):

CO3

Use of practical approach for animal cell culture and its methodology for experiments, analysis, and interpretation of data, in molecular biology

PO2 (3)

Course Objectives

• To highlight the roles and characteristics of microorganisms in field of
Biotechnology
• To impart knowledge on the basic concept of multiplication in
microorganism
• To study in detail the growth, genetic organization of microorganisms and
impact of environment on their growth
• To evaluate explicitly, the metabolic pathways, role of microbes in
public health; insight into the physical and chemical control of
microorganisms.

Learning Outcomes

To impart knowledge on types of microorganisms used in the field of Biotechnology for product development.

Course Syllabus

Module I: Title (4 hrs): Introduction to microbial World

Microbial World; Microscope and its types, Phase contrast microscope, Electron microscope, SEM, TEM, STEM; Microscopic examination of microorganisms.

Practice:Microscope and its use

Module II: Title (5 hrs): Classification of microbes

Classification and Identification of microorganisms; Bacteria, morphology and fine structure of bacteria, cultivation of bacteria, reproduction & growth, pure cultures and cultural characteristics.

Practice:Isolation of bacteria

Module III: Title (4 hrs): Importance of virus in our environment

General characteristics-Morphology and structure of Virus, Classification- isolation and identification-fatal diseases associated with viruses in animals.

Practice:Isolation and Identification of algae, fungi and Protozoa

Module IV: Title (5 hrs): Genetic organization of other microbes and importance

Algae, Fungi, molds and Protozoa – importance, characteristics, morphology, reproduction, physiology cultivation & their association with other organisms. Genome organization of bacteria, virus, algae and fungi,

Practice: Antibiotic Assay - Antimicrobial Sensitivity Test (Disc Diffusion Method)

Module V: Title (4 hrs): Classification of microbes using genome mapping
DNA and RNA present as genetic material in microbes. Types and division of microbes according to their genetic organization. Classification of microbes according to genotyping.

Practice: Growth Kinetics (Bacterial Growth Curve)

Module VI: Title (4 hrs): Metabolism in microbes

Enzymes and their regulation, Microbial metabolism energy production, utilization of energy & biosynthesis, bacterial genetics.

Module VII: Title (4 hrs): Application of microbes in Biotechnology

Application of microbes in fuel industry; Agriculture, aquatic microbiology; Study of domestic water and waste water.

Project: Genome mapping (Partial) of some important industrial bacteria isolated from local area.

Text Books:
1. R.K. Sahoo: Introduction to Microbiology, Year 2020, Kindle publication, Amazon, 1st Edition
2. B. Ray, A. Bhunia: Fundamental food microbiology, CRC press, 5th Edition.

Reference Books:
1. Michael Pelczar JR, E.C.S. Chan, Noel R. Krieg:Microbiology, Mc Graw Hill, 6th Edition.
2. Foster WM, Food Microbiology, CBS publishers, 2018 Edition

Course outline Prepared by; Dr. Ranjan Kumar Sahoo Date:08-06-2020
Source of reference: NSQF/Udemy/ Coursera

Session Plan

Session 1

Introduction to Microbial World

 

Microbial World

Session 1Microbial World PPT

Session 2

Microscope and its types

Types of microscopes

Microscope and its use PPT

Session 3

Practice: Microscope and its use.


Practice: Microscope and its use

Session 4

Phase contrast microscope

Phase-contrast microscope

Session 5

Electron microscope, SEM, TEM, STEM

Electron microscope

Session 6

Classification and Identification of microorganisms

Session 7

Bacteria, morphology and fine structure of bacteria
Fine structure of bacteria


Fine structure of bacteria PPT

Session 8

 

Cultivation of bacteria


Cultivation of bacteria

Session 9

Practice: Isolation of bacteria

 

Practice:Isolation of bacteria

Session 10

Reproduction & growth of bacteria


Reproduction and growth of bacteria PPT

Session 11

Project: Genome mapping (partial) of some industrially important bacteria isolated from local area.

Step 1: Isolation of bacteria from soil.

Session 12

Pure cultures of bacteria

Pure culture of bacteria.

Session 13

Virus and its importance in our life

Viruses

Session 14

General characteristics of virus
Structure of virus

Session 15

Classification- isolation and identification of viruses

Session 16

Fatal diseases associated with viruses in animals

Session 17

Introduction to Algae, Fungi, molds and Protozoa

Session 18

Practice: Isolation and identification of algae, fungi and protozoa


Practice: Isolation and identification of microorganisms

Session 19

Importance of Algae, Fungi, molds and Protozoa in our society

Session 20

Characteristics, morphology, reproduction of Algae, Fungi, molds and protozoa
Morphology of Protists

Session 21

Project: Genome mapping (partial) of some industrially important bacteria isolated from local area.

Step 2: Identification of bacteria isolated from soil.

Session 22

Cultivation & their association with other organisms.

Session 23

Genome organization of bacteria, virus, algae and fungi,
Genome organization

Session 24

Genetic material in microbes

Session 25

Practice: Antibiotic assay (Anti-microbial sensitivity test: Disc diffusion method)


Practice: Antibiotic test

Session 26

DNA and RNA as genetic material in microbes

 

DNA and RNA as genetic material in microbes PPT

Session 27

Classification of microbes according to the genome type. Differences between DNA and RNA present in their chromosome

Session 28

Genotyping of microbes

Session 29

Project: Genome mapping (partial) of some industrially important bacteria isolated from local area.

Step 3: Isolation of DNA from isolated bacteria and gel electrophoresis

Session 30

Enzymes present in microbes

Session 31

Microbial metabolism energy production

Session 32

Practice: Study of growth kinetics of bacteria


Practice

Session 33

Utilization of energy & biosynthesis

Session 34

Bacterial genetics

Session 35

Application of microbes in fuel industry

Session 36

Application of microbes in Agriculture

Session 37

Project: Genome mapping (partial) of some industrially important bacteria isolated from local area.

Step 4: Isolation of DNA from agarose gel.

Session 38

Introduction to Aquatic microbiology

Session 39

Project: Genome mapping (partial) of some industrially important bacteria isolated from local area.

Step 5: Preservation of isolated DNA for further study.

Session 40

Study of microbes in domestic water and waste water.

Session 41

Project: Genome mapping (partial) of some industrially important bacteria isolated from local area.

Review and submission of report

Case Studies

Case Studies

Our Main Teachers

Dr. Ranjan Kumar Sahoo

Associate Professor, Department of Biotechnology
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